Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	123	51.0	1731148	99.3%	1719029	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,220,521	C→T	A343V (GCG→GTG)	patA →	putrescine aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,220,521	0	C	T	97.7%	140.6 / NA	44	A343V (GCG→GTG)	patA	putrescine aminotransferase
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (17/26); total (18/26)
Fisher's exact test for biased strand distribution p-value = 4.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAA  >  NC_000913/3220387‑3220654

tGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTgg                                                                                                                                  >  1:330144/1‑140 (MQ=255)

 gCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTGGc                                                                                                                                 <  2:382276/140‑1 (MQ=255)

 gCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTGGc                                                                                                                                 >  1:314829/1‑140 (MQ=255)

 gCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTGGc                                                                                                                                 <  2:458376/140‑1 (MQ=255)

       gcgcTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTGGCGACCAt                                                                                                                           <  1:308272/140‑1 (MQ=255)

          cTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTGGCGACCATCaa                                                                                                                        >  2:116554/1‑140 (MQ=255)

            cggcggcggCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTGGCGACCATCAAtg                                                                                                                      >  2:566635/1‑140 (MQ=255)

                  cggcgTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTGGCGACCATCAATGTGTTGc                                                                                                                <  1:566635/140‑1 (MQ=255)

                         aTGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTGGCGACCATCAATGTGTTGCTGGAGCa                                                                                                         <  2:519552/140‑1 (MQ=255)

                                ttGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTa                                                                                                  <  2:330144/140‑1 (MQ=255)

                                ttGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTa                                                                                                  <  2:364197/140‑1 (MQ=255)

                                ttGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTa                                                                                                  <  1:595879/140‑1 (MQ=255)

                                   gcgcGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCg                                                                                               >  2:121326/1‑140 (MQ=255)

                                    cgcgACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCgg                                                                                              >  2:496580/1‑140 (MQ=255)

                                    cgcgACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCgg                                                                                              >  2:214312/1‑140 (MQ=255)

                                                     aaGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTCACCGGCTCAGGCTGGGCaaaaa                                                                             >  2:417305/1‑140 (MQ=255)

                                                      agagGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAg                                                                            >  2:289940/1‑140 (MQ=255)

                                                         ggTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCg                                                                         <  2:828893/140‑1 (MQ=255)

                                                          gtgtTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTGGCGAACATCAATTTGTTGCTGGAGCAGAACTTATCGGCTCAGGCTGGGCAAAAAGGCGa                                                                        >  2:611042/1‑140 (MQ=255)

                                                                      cTGTTCGACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGa                                                            <  1:496580/140‑1 (MQ=255)

                                                                      cTGTTCGACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGa                                                            <  2:646888/140‑1 (MQ=255)

                                                                      cTGTTCGACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGa                                                            <  1:214312/140‑1 (MQ=255)

                                                                         ttCGACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACgg                                                         >  1:540254/1‑140 (MQ=255)

                                                                         ttCGACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACgg                                                         >  1:733754/1‑140 (MQ=255)

                                                                          tCGACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGt                                                        <  2:524713/140‑1 (MQ=255)

                                                                                   cATTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTGGCGACCATCAACGTGTTGCTGGAGCAGAACTTACCGGGTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCaa                                               >  1:574555/1‑140 (MQ=255)

                                                                                      tCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTg                                            <  1:116554/140‑1 (MQ=255)

                                                                                      tCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTg                                            >  1:253573/1‑140 (MQ=255)

                                                                                       ccTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTGGCGACCATCAACGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTgg                                           <  2:574555/140‑1 (MQ=255)

                                                                                            aTACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCgg                                      >  2:468825/1‑140 (MQ=255)

                                                                                             tACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCggg                                     <  2:37915/140‑1 (MQ=255)

                                                                                                caccaccTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAat                                  >  1:101487/1‑140 (MQ=255)

                                                                                                caccaccTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAat                                  >  1:410348/1‑140 (MQ=255)

                                                                                                  ccaccTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAatat                                >  1:372215/1‑140 (MQ=255)

                                                                                                  ccaccTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCCGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAatat                                >  1:569869/1‑140 (MQ=255)

                                                                                                    accTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATcc                              <  2:372215/140‑1 (MQ=255)

                                                                                                    accTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATcc                              <  2:253573/140‑1 (MQ=255)

                                                                                                      cTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCg                            <  2:733754/140‑1 (MQ=255)

                                                                                                        ttGGCGGCAACCCGCTGGCCTGTGCGGCGGTGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGAt                          <  2:540254/140‑1 (MQ=255)

                                                                                                          ggcggcAACCCGCTGGCCTGTGCGGCGGTGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCt                        <  1:611042/140‑1 (MQ=255)

                                                                                                          ggcggcAACCCGCTGGCCTGTGCGGCGGTGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCt                        <  1:34237/140‑1 (MQ=255)

                                                                                                          ggcggcAACCCGCTGGCCTGTGCGGCGGTGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCt                        <  2:314829/140‑1 (MQ=255)

                                                                                                            cggcAACCCGCTGGCCTGTGCGGCGGTGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTgg                      <  2:410348/140‑1 (MQ=255)

                                                                                                                           cTGTGCGGCGGTGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTg       <  2:750685/140‑1 (MQ=255)

                                                                                                                             gtgCGGCGGTGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGt     <  2:101487/140‑1 (MQ=255)

                                                                                                                                cggcggtgCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTaaa  <  1:468825/140‑1 (MQ=255)

TGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAA  >  NC_000913/3220387‑3220654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A