Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A23 F60 I1 R1	17	145.5	4959514	98.5%	4885121	138.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,196,232	C→T	T370T (ACC→ACT)	icd →	isocitrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,196,232	0	C	T	100.0%	182.6 / NA	59	T370T (ACC→ACT)	icd	isocitrate dehydrogenase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (32/27); total (32/27)

GGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACAT‑GGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGAT  >  NC_000913/1196095‑1196312

ggTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGa                                                                                >  1:1011276/1‑140 (MQ=255)

ggTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGa                                                                                >  2:572323/1‑140 (MQ=255)

ggTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGa                                                                                >  1:1525592/1‑140 (MQ=255)

  tGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAg                                                                              <  1:572323/140‑1 (MQ=255)

      aaCATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGc                                                                          <  2:1825161/140‑1 (MQ=255)

      aaCATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGc                                                                          >  2:2020113/1‑140 (MQ=255)

      aaCATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGc                                                                          >  1:1370914/1‑140 (MQ=255)

       aCATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCt                                                                         <  1:1618013/140‑1 (MQ=255)

         aTCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGa                                                                       >  2:223781/1‑140 (MQ=255)

          tCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAc                                                                      <  2:1525592/140‑1 (MQ=255)

          tCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAc                                                                      <  2:1607978/140‑1 (MQ=255)

          tCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAc                                                                      <  1:2020113/140‑1 (MQ=255)

          tCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAc                                                                      <  1:1063905/140‑1 (MQ=255)

           cGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAcc                                                                     >  2:1786139/1‑139 (MQ=255)

           cGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAcc                                                                     >  1:1400644/1‑139 (MQ=255)

           cGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAcc                                                                     >  2:775379/1‑139 (MQ=255)

           cGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAcc                                                                     >  2:1899858/1‑139 (MQ=255)

            ggTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGCAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCCCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCgg                                                                           >  2:881020/1‑133 (MQ=255)

            ggTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCgg                                                                           <  1:881020/133‑1 (MQ=255)

            ggTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAcct                                                                    >  2:1266501/1‑138 (MQ=255)

            ggTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAcct                                                                    >  1:186331/1‑138 (MQ=255)

             gTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAcctg                                                                   <  1:1696246/140‑4 (MQ=255)

             gTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAcctg                                                                   <  1:223781/140‑4 (MQ=255)

              tGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAcctga                                                                  <  2:1370914/140‑5 (MQ=255)

                aCGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGAtt                                                                >  1:2373483/1‑140 (MQ=255)

                   aaTGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAcctg                                                                   <  1:276705/134‑4 (MQ=255)

                   aaTGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAcctg                                                                   >  2:276705/1‑131 (MQ=255)

                    aTGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTa                                                            <  1:775379/140‑1 (MQ=38)

                    aTGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTa                                                            <  1:1102737/140‑1 (MQ=38)

                    aTGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTa                                                            <  1:1786139/140‑1 (MQ=255)

                        gcCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAgg                                                        >  2:2466304/1‑140 (MQ=255)

                         cccTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGt                                                       <  1:1922435/140‑1 (MQ=37)

                             gTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATgg                                                   >  2:2011025/1‑140 (MQ=255)

                             gTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATgg                                                   >  2:1049709/1‑140 (MQ=255)

                                        cccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATc                                        >  1:1036089/1‑140 (MQ=255)

                                        cccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATc                                        >  2:520115/1‑140 (MQ=255)

                                        cccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATc                                        >  2:274033/1‑140 (MQ=255)

                                        cccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATc                                        <  1:79566/140‑1 (MQ=25)

                                          cacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCa                                       <  2:1368856/140‑1 (MQ=25)

                                              gTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgcc                                  <  1:1558048/140‑2 (MQ=21)

                                              gTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgcc                                  <  1:2466304/140‑2 (MQ=21)

                                                 cTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCaag                               >  1:1688271/1‑139 (MQ=21)

                                                   gcgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGgcg                                             <  2:1713433/124‑1 (MQ=25)

                                                   gcgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGgcg                                             >  1:1713433/1‑124 (MQ=255)

                                                   gcgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaatgcc                                  <  2:513605/135‑5 (MQ=21)

                                                   gcgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgcc                                  >  1:513605/1‑134 (MQ=21)

                                                   gcgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGAc                             >  2:149188/1‑140 (MQ=18)

                                                   gcgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGAc                             >  1:140417/1‑140 (MQ=18)

                                                    cgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGAcc                            >  1:2335776/1‑140 (MQ=18)

                                                    cgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGAcc                            >  2:857784/1‑140 (MQ=18)

                                                     gcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCg                           <  2:2335776/140‑1 (MQ=18)

                                                       cGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTa                         >  1:1062565/1‑140 (MQ=18)

                                                         aaaTATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAAc                       >  1:1703515/1‑140 (MQ=18)

                                                               gCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGa                 <  2:1400644/140‑1 (MQ=17)

                                                                   gTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTc             >  2:1730819/1‑140 (MQ=17)

                                                                   gTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTc             >  1:1495979/1‑140 (MQ=17)

                                                                     cAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGa           <  1:1049709/140‑1 (MQ=14)

                                                                     cAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGa           <  2:1489237/140‑1 (MQ=14)

                                                                       ggACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGaac         <  1:857784/140‑3 (MQ=14)

                                                                              gTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTgat  >  2:2155257/1‑140 (MQ=11)

GGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACAT‑GGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGAT  >  NC_000913/1196095‑1196312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A