Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F60 I1 R1	22	49.8	1704528	98.6%	1680664	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,383,216	A→C	F249C (TTT→TGT)	ycjW ←	putative LacI‑type DNA‑binding transcriptional regulator YcjW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,383,216	0	A	C	100.0%	93.6 / NA	26	F249C (TTT→TGT)	ycjW	putative LacI‑type DNA‑binding transcriptional regulator YcjW
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (15/11); total (15/11)

GCCGCCCGGTGCAGGCGTTAAAATAAATGACAGCGTTTCATTGCTATAACCCACCACCGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAGTTTCATGGGTATAACCGCCATCAACAATCCATTCATCACGCACTGCAATATTATGCGCAGCCAGGCTCTGCTTAT  >  NC_000913/1383080‑1383348

gccgccCGGTGCAGGCGTTAAAATAAATGACAGCGTTTCATTGCTATAACCCACCACCGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGcacaca                                                                                                                                   <  2:49549/140‑1 (MQ=255)

gccgccCGGTGCAGGCGTTAAAATAAATGACAGCGTTTCATTGCTATAACCCACCACCGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGcacaca                                                                                                                                   <  1:619773/140‑1 (MQ=255)

 ccgccCGGTGCAGGCGTTAAAATAAATGACAGCGTTTCATTGCTATAACCCACCACCGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCacacac                                                                                                                                  >  2:83894/1‑140 (MQ=255)

             ggCGTTAAAATAAATGACAGCGTTTCATTGCTATAACCCACCACCGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCACACACCGCCTCAGGCaa                                                                                                                      <  1:614194/140‑1 (MQ=255)

                  tAAAATAAATGACAGCGTTTCATTGCTATAACCCACCACCGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCACACACCGCCTCAGGCAACGGTg                                                                                                                 <  2:365352/140‑1 (MQ=255)

                   aaaaTAAATGACAGCGTTTCATTGCTATAACCCACCGCCGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCACAAACCGCCTCAGGCAACGGTGa                                                                                                                <  2:76154/140‑1 (MQ=255)

                   aaaaTAAATGACAGCGTTTCATTGCTATAACCCACCACCGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCACACACCGcc                                                                                                                              >  1:343531/1‑126 (MQ=255)

                   aaaaTAAATGACAGCGTTTCATTGCTATAACCCACCACCGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCACACACCGcc                                                                                                                              <  2:343531/126‑1 (MQ=255)

                   aaaaTAAATGACAGCGTTTCATTGCTATAACCCACCACCGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCACACACCGCCTCAGGCAACGGTGa                                                                                                                >  1:319632/1‑140 (MQ=255)

                   aaaaTAAATGACAGCGTTTCATTGCTATAACCCACCACCGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCACACACCGCCTCAGGCAACGGTGa                                                                                                                >  1:726164/1‑140 (MQ=255)

                   aaaaTAAATGACAGCGTTTCATTGCTATAACCCACCACCGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCACACACCGCCTCAGGCAACGGTGa                                                                                                                <  1:631538/140‑1 (MQ=255)

                      ataaatGACAGCGTTTCATTGCTATAACCCACCACCGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCACACACCGCCTCAGGCAACGGTGACTg                                                                                                             <  2:10129/140‑1 (MQ=255)

                          atGACAGCGTTTCATTGCTATAACCCACCACCGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCACACACCGCCTCAGGCAACGGTGACTGGCtt                                                                                                         >  2:256319/1‑140 (MQ=255)

                                   tttCATTGCTATAACCCACCACCTCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCACACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGc                                                                                                >  2:620796/1‑140 (MQ=255)

                                         tGCTATAACCCACCACCGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCACACACCGCCTCAGGCAACGGTGACTGGCTtaat                                                                                                      >  1:135575/1‑128 (MQ=255)

                                         tGCTATAACCCACCACCGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCACACACCGCCTCAGGCAACGGTGACTGGCTtaat                                                                                                      <  2:135575/128‑1 (MQ=255)

                                           cTATAACCCACCACCGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCACACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGtgctgc                                                                                        <  1:235495/140‑1 (MQ=255)

                                                    accaccGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCACACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGc                                                                               <  1:83894/140‑1 (MQ=255)

                                                        ccGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCACACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAGtt                                                                           >  2:689222/1‑140 (MQ=255)

                                                                     cGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCACACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAGTTTCATGGGTATAAc                                                              >  1:24342/1‑140 (MQ=255)

                                                                               atatTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCACACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAGTTTCATGGGTATAACCGCCATcaac                                                    >  2:329400/1‑140 (MQ=255)

                                                                                tatTTTTCTCTGCCCCCGCACGATAAATCCTCATTAATTTCAGCCTGTCAGTGGCCCACCCCCCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAGTTTCATGGGTATAACCGCCATcaaca                                                   <  2:487954/140‑1 (MQ=255)

                                                                                          tgctgcCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCACACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAGTTTCATGGGTATAACCGCCATCAACAATCCATtcat                                         >  2:478794/1‑140 (MQ=255)

                                                                                           gctgcCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCACACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAGTTTCATGGGTATAACCGCCATCAACAATCCATtcatc                                        <  1:765969/140‑1 (MQ=255)

                                                                                           gctgcCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCACACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAGTTTCATGGGTATAACCGCCATCAACAATCCATtcatc                                        >  1:407426/1‑140 (MQ=255)

                                                                                            ctgcCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCACACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAGTTTCATGGGTATAACCGCCATCAACAATCCATtcatca                                       >  1:656381/1‑140 (MQ=255)

                                                                                              gcCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCACACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAGTTTCATGGGTATAACCGCCATCAACAATCCATTCATCACg                                     <  1:583176/140‑1 (MQ=255)

                                                                                                cGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCACACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAGTTTCATGGGTATAACCGCCATCAACAATCCATTCATCACGCa                                   <  1:689222/140‑1 (MQ=255)

                                                                                                  cACGATAAATGCTCATTATTTTCAGGCTGTCAGTGGCACCCCCCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAGTTTCATGGGTATAACCGCCATCAACAATCCATTCATCACGCACt                                 <  2:319632/140‑1 (MQ=255)

                                                                                                     gATAAATGCTCATTATTTTCAGGCTGTCAGTGGCACCCCCCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAGTTTCATGGGTATAACCGCCATCAACAATCCATTCATCACGCACTGCa                              <  2:656381/140‑1 (MQ=255)

                                                                                                             cTCATTAATTTCAGGCTGTCAGTGGCACACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAGTTTCATGGGTATAACCGCCATCAACAATCCATTCATCACGCACTGCAATATTATg                      <  2:24342/140‑1 (MQ=255)

                                                                                                               cATTAATTTCAGGCTGTCAGTGGCACACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAGTTTCATGGGTATAACCGCCATCAACAATCCATTCATCACGCACTGCAATATTATgcg                    >  2:466423/1‑140 (MQ=255)

                                                                                                                       tCAGGCTGTCAGTGGCACACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAGTTTCATGGGTATAACCGCCATCAACAATCCATTCATCACGCACTGCAATATTATGCGCAGCCAgg            <  2:726164/140‑1 (MQ=255)

                                                                                                                         aGGCTGTCAGTGGCACACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAGTTTCATGGGTATAACCGCCATCAACAATCCATTCATCACGCACTGCAATATTATGCGCAGCCAGGct          <  1:466423/140‑1 (MQ=255)

                                                                                                                         aGGCTGTCAGTGGCACACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAGTTTCATGGGTATAACCGCCATCAACAATCCATTCATCACGCACTGCAATATTATGCGCAGCCAGGct          <  2:407426/140‑1 (MQ=255)

                                                                                                                              gTCAGTGGCACACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAGTTTCATGGGTATAACCGCCATCAACAATCCATTCATCACGCACTGCAATATTATGCGCAGCCAGGCTCTGCt     >  1:498039/1‑140 (MQ=255)

                                                                                                                               tCAGTGGCACACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAGTTTCATGGGTATAACCGCCATCAACAATCCATTCATCACGCACTGCAATATTATGCGCAGCCAGGCTCTGCtt    >  1:540530/1‑140 (MQ=255)

                                                                                                                                 aGTGGCACACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAGTTTCATGGGTATAACCGCCATCAACAATCCATTCATCACGCACTGCAATATTATGCGCAGCCAGGCTCTGCTtat  <  1:9200/140‑1 (MQ=255)

GCCGCCCGGTGCAGGCGTTAAAATAAATGACAGCGTTTCATTGCTATAACCCACCACCGCTAACTGCTGCGGAATAGCAATATTTTTCTCTGCTGCCGCACGATAAATGCTCATTAATTTCAGGCTGTCAGTGGCAAACACCGCCTCAGGCAACGGTGACTGGCTTAATAATTGCCGTGCTGCTTTTAATGCAGTTTCATGGGTATAACCGCCATCAACAATCCATTCATCACGCACTGCAATATTATGCGCAGCCAGGCTCTGCTTAT  >  NC_000913/1383080‑1383348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A