Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A19 F60 I1 R1	24	40.0	1367016	98.7%	1349244	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,619,534	C→A	L139I (CTT→ATT)	marR →	DNA‑binding transcriptional repressor MarR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,619,534	0	C	A	100.0%	109.2 / NA	33	L139I (CTT→ATT)	marR	DNA‑binding transcriptional repressor MarR
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (20/13); total (20/13)

CGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGACAACCTGGAATCGCCACTGTCACTGGAGAAAGTGTCA  >  NC_000913/1619401‑1619669

cggcgTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGATTAAGa                                                                                                                                   <  1:471089/140‑1 (MQ=255)

             aaaCTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGATTAAGAAAGTCCTGCCGTa                                                                                                                      >  2:207340/1‑140 (MQ=255)

                    ccaccGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGATTAAGAAAGTCCTGCCGTAAACaaaa                                                                                                               >  1:627796/1‑140 (MQ=255)

                      accGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGATTAAGAAAGTCCTGCCGTAAACaaaaaa                                                                                                             <  1:200039/140‑1 (MQ=255)

                         ggcggcGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGATTAAGAAAGTCCTGCCGTAAACAAAAAagag                                                                                                          >  1:233066/1‑140 (MQ=255)

                                 ggcAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGATTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACg                                                                                                  <  1:122459/140‑1 (MQ=255)

                                        tgtgAACAATGCAATAAATTAGTTGGCCAGGACCTGCACCAAGAATTACAAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGATTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCa                                                                                           <  2:233066/140‑1 (MQ=255)

                                             aCAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGATTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGc                                                                                      >  2:145746/1‑140 (MQ=255)

                                             aCAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGATTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGc                                                                                      >  1:200544/1‑140 (MQ=255)

                                                aTGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGATTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAAt                                                                                   >  2:315595/1‑140 (MQ=255)

                                                 tGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGATTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATa                                                                                  >  1:84348/1‑140 (MQ=255)

                                                 tGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGATTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATa                                                                                  >  1:516485/1‑140 (MQ=255)

                                                 tGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGATTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATa                                                                                  >  2:355833/1‑140 (MQ=255)

                                                     aTCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGATTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGa                                                                              <  1:355833/140‑1 (MQ=255)

                                                     aTCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGATTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGa                                                                              <  1:315595/140‑1 (MQ=255)

                                                       cAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGATTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACg                                                                            <  2:627796/140‑1 (MQ=255)

                                                                ggCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGATTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCa                                                                   >  1:344430/1‑140 (MQ=255)

                                                                ggCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGATTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCa                                                                   >  1:134743/1‑140 (MQ=255)

                                                                            cACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGATTAAGAAAGTCCTGCCTTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCAttt                                                       >  1:302566/1‑140 (MQ=255)

                                                                                aaGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGATTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGa                                                   >  2:68291/1‑140 (MQ=255)

                                                                                 aGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGATTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGAc                                                  >  1:519908/1‑140 (MQ=255)

                                                                                           aaaaaCCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGATTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAgg                                        <  1:145746/140‑1 (MQ=255)

                                                                                           aaaaaCCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGATTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAgg                                        <  2:84348/140‑1 (MQ=255)

                                                                                             aaaCCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGATTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGCATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGAc                                      >  1:316659/1‑140 (MQ=255)

                                                                                                    aCGGCGGACGAAGTGGCAACACTTGAGTATTTGATTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGACAACCTgg                               >  2:339582/1‑140 (MQ=255)

                                                                                                          gACGAAGTGGCAACACTTGAGTATTTGATTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGACAACCTGGAATCGc                         >  1:506136/1‑140 (MQ=255)

                                                                                                                 tGGCAACACTTGAGTATTTGATTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGACAACCTGGAATCGCCACTGTc                  <  2:134743/140‑1 (MQ=255)

                                                                                                                   gCAACACTTGAGTATTTGATTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGACAACCTGGAATCGCCACTGTCAc                <  1:207340/140‑1 (MQ=255)

                                                                                                                   gCAACACTTGAGTATTTGATTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGACAACCTGGAATCGCCACTGTCAc                <  2:519908/140‑1 (MQ=255)

                                                                                                                    cAACACTTGAGTATTTGATTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGACAACCTGGAATCGCCACTGTCACt               >  2:603832/1‑140 (MQ=255)

                                                                                                                       cacTTGAGTATTTGATTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGACAACCTGGAATCGCCACTGTCACTGga            <  1:603832/140‑1 (MQ=255)

                                                                                                                               tATTTGATTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGACAACCTGGAATCGCCACTGTCACTGGAGAAAgtgt    >  2:312422/1‑140 (MQ=255)

                                                                                                                                 tttGATTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGACAACCTGGAATCGCCACTGTCACTGGAGAAAGTGTCa  <  1:378427/140‑1 (MQ=255)

                                                                                                                                 tttGATTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGACAACCTGGAATCGCCACTGTCACTGGAGAAAGTGTCa  >  1:183625/1‑140 (MQ=255)

                                                                                                                                 tttGATAAAGAAATCCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCAAGGACAACCTGGAATCGCCACTGTCACTGGAGAAAGTGTCa  <  2:200544/140‑1 (MQ=255)

CGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACTGGATCGAGGACAACCTGGAATCGCCACTGTCACTGGAGAAAGTGTCA  >  NC_000913/1619401‑1619669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A