Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A34 F60 I1 R1	14	117.0	3967004	98.6%	3911465	138.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,239,037	Δ1 bp	coding (159/1071 nt)	dadX →	alanine racemase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,239,036	0	C	.	100.0%	471.6 / NA	111	coding (158/1071 nt)	dadX	alanine racemase 2
Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (52/59); total (52/59)

Only 100 of 122 total aligned reads displayed.

GCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCACCGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCACCGCCTGACCA  >  NC_000913/1238897‑1239170

gCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCAc                                                                                                                                        >  1:1341843/1‑140 (MQ=255)

gCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCAc                                                                                                                                        >  2:187286/1‑140 (MQ=255)

gCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCAc                                                                                                                                        >  2:172346/1‑140 (MQ=255)

gCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCAc                                                                                                                                        <  2:1592556/140‑1 (MQ=255)

    gCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCAcgatg                                                                                                                                    >  2:260405/1‑136 (MQ=255)

      cTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGc                                                                                                                                 <  2:579302/140‑1 (MQ=255)

       tCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCt                                                                                                                                <  1:538375/140‑1 (MQ=255)

        cGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCtt                                                                                                                               >  1:1639034/1‑140 (MQ=255)

          aTCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTg                                                                                                                             >  2:1696896/1‑140 (MQ=255)

          aTCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTg                                                                                                                             >  1:15604/1‑140 (MQ=255)

           tCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAACGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGc                                                                                                                            <  2:1639034/140‑1 (MQ=255)

           tCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGc                                                                                                                            <  2:1843066/140‑1 (MQ=255)

           tCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGc                                                                                                                            <  1:669550/140‑1 (MQ=255)

               cAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTg                                                                                                                        >  1:1633845/1‑140 (MQ=255)

               cAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTg                                                                                                                        >  2:1440846/1‑140 (MQ=255)

               cAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTg                                                                                                                        >  1:883274/1‑140 (MQ=255)

                aGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGc                                                                                                                       <  2:786345/140‑1 (MQ=255)

                 ggCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCt                                                                                                                      <  1:937078/140‑1 (MQ=255)

                 ggCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCGTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑AGATGGCTTTGCATTGCt                                                                                                                      <  2:484089/140‑1 (MQ=255)

                  gCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCt                                                                                                                      <  1:1167094/139‑1 (MQ=255)

                  gCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCt                                                                                                                      >  2:1167094/1‑139 (MQ=255)

                    aTTAAAACAGAATCTGTCCATTGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCt                                                                                                                      >  1:1722254/1‑137 (MQ=255)

                       aaaaCAGAATCTGTCCATTGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCt                                                                                                                >  1:788383/1‑140 (MQ=255)

                            aGAATCTGTCCATTGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGCCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAag                                                                                                           >  2:328204/1‑140 (MQ=255)

                             gAATCTGTCCATTGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAaga                                                                                                          <  1:260405/140‑1 (MQ=255)

                              aaTCTGTCCATTGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAagag                                                                                                         >  1:22404/1‑140 (MQ=255)

                              aaTCTGTCCATTGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAagag                                                                                                         >  1:1910480/1‑140 (MQ=255)

                                tCTGTCCATTGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGc                                                                                                       >  2:446796/1‑140 (MQ=255)

                                 cTGTCCATTGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCa                                                                                                      <  2:15604/140‑1 (MQ=255)

                                   gTCCATTGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAAt                                                                                                    <  1:1124558/140‑1 (MQ=255)

                                   gTCCATTGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAAt                                                                                                    >  2:676242/1‑140 (MQ=255)

                                   gTCCATTGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAAt                                                                                                    <  1:1937055/140‑1 (MQ=255)

                                    tCCATTGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCa                                                                                                      >  2:1663852/1‑137 (MQ=255)

                                    tCCATTGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCa                                                                                                      <  1:1663852/137‑1 (MQ=255)

                                     ccATTGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATaa                                                                                                  <  1:170966/140‑1 (MQ=255)

                                         tGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGtt                                                                                              >  2:1652035/1‑140 (MQ=255)

                                         tGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGtt                                                                                              >  1:140870/1‑140 (MQ=255)

                                           tCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTAc                                                                                            >  2:1541530/1‑140 (MQ=255)

                                              gCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTg                                                                                         >  1:1695731/1‑140 (MQ=255)

                                              gCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTg                                                                                         >  2:4417/1‑140 (MQ=255)

                                                cAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAg                                                                                       <  1:328204/140‑1 (MQ=255)

                                                cAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAg                                                                                       >  2:904428/1‑140 (MQ=255)

                                                     cgcgACGCACGCGCGGGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCgg                                                                                  <  2:1885290/140‑1 (MQ=255)

                                                     cgcgACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCgg                                                                                  <  2:249070/140‑1 (MQ=255)

                                                     cgcgACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCgg                                                                                  <  2:883274/140‑1 (MQ=255)

                                                     cgcgACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCgg                                                                                  >  2:108825/1‑140 (MQ=255)

                                                      gcgACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGc                                                                                 <  1:1440846/140‑1 (MQ=255)

                                                       ggACGCACGCGCGCGGCTGGTCGGGGGTAAAAGCGAACGCCTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCt                                                                                <  2:1279871/139‑1 (MQ=255)

                                                           gcacgcGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGaa                                                                            <  1:676242/140‑1 (MQ=255)

                                                            cacgcGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGaaa                                                                           >  1:1931950/1‑140 (MQ=255)

                                                             acgcGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAg                                                                          <  2:1954234/140‑1 (MQ=255)

                                                              cgcgcgcgTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAgg                                                                         <  1:1652035/140‑1 (MQ=255)

                                                                 gcgcgTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAgcgc                                                                                    <  2:416324/126‑1 (MQ=255)

                                                                 gcgcgTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAgcgc                                                                                    >  1:416324/1‑126 (MQ=255)

                                                                               tggtAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTgg                                                        <  1:4417/140‑1 (MQ=255)

                                                                                ggtAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGa                                                       <  1:1371307/140‑1 (MQ=255)

                                                                                 gtAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGaa                                                      >  2:1158096/1‑140 (MQ=255)

                                                                                      aGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGAtt                                                 <  1:1541530/140‑1 (MQ=255)

                                                                                         gAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTgg                                                        <  2:1407907/130‑1 (MQ=255)

                                                                                           aCGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTcc                                            >  2:950244/1‑140 (MQ=255)

                                                                                            cGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCa                                           >  1:547551/1‑140 (MQ=255)

                                                                                                 aCGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTc                                      <  1:172346/140‑1 (MQ=255)

                                                                                                 aCGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTc                                      >  1:459894/1‑140 (MQ=255)

                                                                                                  cGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCa                                     <  1:950244/140‑1 (MQ=255)

                                                                                                  cGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCa                                     <  2:1161213/140‑1 (MQ=255)

                                                                                                  cGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCa                                     <  2:1054290/140‑1 (MQ=255)

                                                                                                       aTGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATc                                >  2:760072/1‑140 (MQ=255)

                                                                                                         ggTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCa                                           <  1:564967/127‑1 (MQ=255)

                                                                                                         ggTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCa                                           >  2:564967/1‑127 (MQ=255)

                                                                                                         ggTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCa                                           >  2:866012/1‑127 (MQ=255)

                                                                                                         ggTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCa                                           <  1:866012/127‑1 (MQ=255)

                                                                                                         ggTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCa                                     >  1:701094/1‑133 (MQ=255)

                                                                                                         ggTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCa                                     <  2:701094/133‑1 (MQ=255)

                                                                                                         ggTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTg                              >  2:1555397/1‑140 (MQ=255)

                                                                                                         ggTATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTg                              <  2:1341843/140‑1 (MQ=255)

                                                                                                           tATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGga                            <  1:368419/140‑1 (MQ=255)

                                                                                                           tATTGAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGga                            <  2:140870/140‑1 (MQ=255)

                                                                                                               gAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGga                            <  1:1961946/136‑1 (MQ=255)

                                                                                                               gAGCGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGGAGAtt                        >  1:1870288/1‑140 (MQ=255)

                                                                                                                  cGTATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTAt                     >  2:472617/1‑140 (MQ=255)

                                                                                                                    tATCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGa                   >  2:1860097/1‑140 (MQ=255)

                                                                                                                     aTCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGga                            <  1:986191/130‑1 (MQ=255)

                                                                                                                     aTCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGga                            >  2:1360661/1‑130 (MQ=255)

                                                                                                                     aTCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGga                            <  1:1360661/130‑1 (MQ=255)

                                                                                                                     aTCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGAc                  >  1:54085/1‑140 (MQ=255)

                                                                                                                     aTCTGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGAc                  >  2:345546/1‑140 (MQ=255)

                                                                                                                        tGGAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAg               <  1:53215/140‑1 (MQ=255)

                                                                                                                         ggAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGc              <  1:345546/140‑1 (MQ=255)

                                                                                                                         ggAGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGc              <  2:1871294/140‑1 (MQ=255)

                                                                                                                           aGCGCGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCAc            <  1:792273/140‑1 (MQ=255)

                                                                                                                            gcgcGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCAcc           <  2:547551/140‑1 (MQ=255)

                                                                                                                            gcgcGATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCAcc           <  1:1466363/140‑1 (MQ=255)

                                                                                                                               cgATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGc              <  2:662924/134‑1 (MQ=255)

                                                                                                                               cgATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGc              >  1:662924/1‑134 (MQ=255)

                                                                                                                               cgATCGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCACCGcc        >  2:1791256/1‑140 (MQ=255)

                                                                                                                                   cGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCACCGCCTGac    <  2:54085/140‑1 (MQ=255)

                                                                                                                                   cGGGGCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCACCGCCTGac    <  1:446796/140‑1 (MQ=255)

                                                                                                                                     gggCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCACCGCCTGacca  <  2:1695731/140‑1 (MQ=255)

                                                                                                                                     gggCCA‑CGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCACCGCCTGacca  <  1:904428/140‑1 (MQ=255)

                                                                                                                                      ggCCACCGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGGTACGTGAGCGCGGCTGGAAAGGATCGATCCTGATGCTGGAAGGATTTTGCCATGCTCAGGATCTGGAGATTTATGACTAGTACCGCCTAaccg  <  2:1873416/140‑2 (MQ=255)

GCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCGCGACGCACGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGATCGGGGCCACCGATGGCTTTGCATTGCTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAAGGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCACCGCCTGACCA  >  NC_000913/1238897‑1239170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A