Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A37 F60 I1 R1	21	79.1	2697330	98.7%	2662264	138.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,156,086	G→T	A109S (GCG→TCG)	holB →	DNA polymerase III subunit delta'

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,156,086	0	G	T	100.0%	181.9 / NA	52	A109S (GCG→TCG)	holB	DNA polymerase III subunit delta'
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (23/29); total (23/29)

GGATGTCAGTTGATGCAGGCTGGCACGCATCCCGATTACTACACCCTGGCTCCCGAAAAAGGAAAAAATACGCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTGCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCGGCTAACGCATTGCTGAAAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCA  >  NC_000913/1155951‑1156220

ggATGTCAGTTGATGCAGGCTGGCACGCATCCCGATTACTACACCCTGGCTCCCGAAAAAGGAAAAAATACGCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGaa                                                                                                                                    <  1:716621/140‑1 (MQ=255)

    gTCAGTTGATGCAGGCTGGCACGCATCCCGATTACTACACCCTGGCTCCCGAAAAAGTAAAAAATACGCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTc                                                                                                                                >  1:761216/1‑140 (MQ=255)

      cAGTTGATGCAGGCTGGCACGCATCCCGATTACTACACCCTGGCTCCCGAAAAAGGAAAAAATACGCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGt                                                                                                                              >  2:847922/1‑140 (MQ=255)

      cAGTTGATGCAGGCTGGCACGCATCCCGATTACTACACCCTGGCTCCCGAAAAAGGAAAAAATACGCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGt                                                                                                                              >  1:635999/1‑140 (MQ=255)

                    tGGCACGCATCCCGATTACTACACCCTGGCTCCCGAAAAAGGAAAAAATACGCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGAtg                                                                                                                <  1:973774/140‑1 (MQ=255)

                    tGGCACGCATCCCGATTACTACACCCTGGCTCCCGAAAAAGGAAAAAATACGCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGAtg                                                                                                                <  1:770647/140‑1 (MQ=255)

                      gCACGCATCCCGATTACTACACCCTGGCTCCCGAAAAAGGAAAAAATACGCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGAtgct                                                                                                              <  1:457284/140‑1 (MQ=255)

                      gCACGCATCCCGATTACTACACCCTGGCTCCCGAAAAAGGAAAAAATACGCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGAtgct                                                                                                              <  1:489958/140‑1 (MQ=255)

                            aTCCCGATTACTACACCCTGGCTCCCGAAAAAGGAAAAAATACGCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTa                                                                                                        >  1:246745/1‑140 (MQ=255)

                            aTCCCGATTACTACACCCTGGCTCCCGAAAAAGGAAAAAATACGCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTa                                                                                                        <  2:635999/140‑1 (MQ=255)

                            aTCCCGATTACTACACCCTGGCTCCCGAAAAAGGAAAAAATACGCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTa                                                                                                        >  2:844291/1‑140 (MQ=255)

                            aTCCCGATTACTACACCCTGGCTCCCGAAAAAGGAAAAAATACGCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTa                                                                                                        <  1:813544/140‑1 (MQ=255)

                             tCCCGATTACTACACCCTGGCTCCCGAAAAAGGAAAAAATACGCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTaa                                                                                                                      <  2:798911/125‑1 (MQ=255)

                             tCCCGATTACTACACCCTGGCTCCCGAAAAAGGAAAAAATACGCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTaa                                                                                                                      >  1:798911/1‑125 (MQ=255)

                                        acacCCTGGCTCCCGAAAAAGGAAAAAATACGCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGAcgcc                                                                                            >  1:470918/1‑140 (MQ=255)

                                        acacCCTGGCTCCCGAAAAAGGAAAAAATACGCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGAcgcc                                                                                            <  2:470918/140‑1 (MQ=255)

                                            ccTGGCTCCCGAAAAAGGAAAAAATACGCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCgg                                                                                        >  2:1134666/1‑140 (MQ=255)

                                             cTGGCTCCCGAAAAAGGAAAAAATACGCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCGGc                                                                                       <  2:878625/140‑1 (MQ=255)

                                             cTGGCTCCCGAAAAAGGAAAAAATACGCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCGGc                                                                                       <  1:847922/140‑1 (MQ=255)

                                              tGGCTCCCGAAAAAGGAAAAAATACGCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCGGCt                                                                                      >  1:1287928/1‑140 (MQ=255)

                                              tGGCTCCCGAAAAAGGAAAAAATACGCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCGGCt                                                                                      <  1:844291/140‑1 (MQ=255)

                                              tGGCTCCCGAAAAAGGAAAAAATACGCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCGGCt                                                                                      >  1:770529/1‑140 (MQ=255)

                                               ggCTCCCGAAAAAGGAAAAAATACGCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCGGc                                                                                       >  1:1297037/1‑138 (MQ=255)

                                               ggCTCCCGAAAAAGGAAAAAATACGCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCGGc                                                                                       <  2:1297037/138‑1 (MQ=255)

                                                gCTCCCGAAAAAGGAAAAAATACGCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCGGCTaa                                                                                    >  1:870605/1‑140 (MQ=255)

                                                   cccGAAAAAGGAAAAAATACGCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCGACTAACGc                                                                                 <  1:725861/140‑1 (MQ=255)

                                                    ccGAAAAAGGAAAAAATACGCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCGGCTAACGCa                                                                                <  2:733659/140‑1 (MQ=255)

                                                     cGAAAAAGGAAAAAATACGCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGAcgccgc                                                                                          <  1:529601/129‑1 (MQ=255)

                                                     cGAAAAAGGAAAAAATACGCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGAcgccgc                                                                                          >  2:529601/1‑129 (MQ=255)

                                                            ggAAAAAATACGCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCGGCTAACGCATTGCTGaa                                                                        <  2:46094/140‑1 (MQ=255)

                                                              aaaaaaTACGCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCGGCTAACGCATTGCTGaaaa                                                                      <  1:782245/140‑1 (MQ=255)

                                                                   aTACGCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCGGCTAACGCATTGCTGAAAACGCtt                                                                 <  1:895244/140‑1 (MQ=255)

                                                                   aTACGCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCGGCTAACGCATTGCTGAAAACGCtt                                                                 <  2:159660/140‑1 (MQ=255)

                                                                   aTACGCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCGGCTAACGCATTGCTGAAAACGCtt                                                                 <  2:770529/140‑1 (MQ=255)

                                                                       gCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCGGCTAACGCATTGCt                                                                           >  2:1177943/1‑126 (MQ=255)

                                                                       gCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCGGCTAACGCATTGCt                                                                           <  1:1177943/126‑1 (MQ=255)

                                                                          gggCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCGGCTAACGCATTGCTGAAAACGCTTGAAGAGc                                                          >  2:126969/1‑140 (MQ=255)

                                                                           ggCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCGGCTAACGCATTGCTGAAAACGCTTGAAGAGcc                                                         >  1:45481/1‑140 (MQ=255)

                                                                            gCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCGGCTAACGCATTGCTGaa                                                                        >  1:237820/1‑124 (MQ=255)

                                                                            gCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCGGCTAACGCATTGCTGaa                                                                        <  2:237820/124‑1 (MQ=255)

                                                                            gCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCGGCTAACGCATTGCTGAAAACGCTTGAAGAGcca                                                        >  1:1279940/1‑140 (MQ=255)

                                                                                    gCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCGGCTAACGCATTGCTGAAAACGCTTGAAGAGCCACCAGCAGa                                                <  2:246745/140‑1 (MQ=255)

                                                                                                  cACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCGGCTAACGCATTGCTGAAAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTcc                                  >  2:108103/1‑140 (MQ=255)

                                                                                                  cACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCGGCTAACGCATTGCTGAAAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTcc                                  >  2:331085/1‑140 (MQ=255)

                                                                                                            cTGAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCGGCTAACGCATTGCTGAAAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCcg                        <  1:126969/140‑1 (MQ=255)

                                                                                                              gAATGAGCACGCACGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCGGCTAACGCATTGCTGAAAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCcgcg                      >  1:143049/1‑140 (MQ=255)

                                                                                                                    gcacgcacGCTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCGGCTAACGCATTGCTGAAAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTg                <  2:1279940/140‑1 (MQ=255)

                                                                                                                       cgcacgcTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCGGCTAACGCATTGCTGAAAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAAc             >  1:867483/1‑140 (MQ=255)

                                                                                                                             cTTAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCGGCTAACGCATTGCTGAAAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTAc       >  2:96157/1‑140 (MQ=255)

                                                                                                                              ttAGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCGGCTAACGCATTGCTGAAAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACt      <  2:870605/140‑1 (MQ=255)

                                                                                                                                aGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCGGCTAACGCATTGCTGAAAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTgg    <  2:45481/140‑1 (MQ=255)

                                                                                                                                aGGTGGTTCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCGGCTAACGCATTGCTGAAAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTgg    <  2:867483/140‑1 (MQ=255)

                                                                                                                                  gtggttCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCGGCTAACGCATTGCTGAAAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCa  <  2:761216/140‑1 (MQ=255)

                                                                                                                                  gtggttCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCGGCTAACGCATTGCTGAAAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCa  >  2:153524/1‑140 (MQ=255)

GGATGTCAGTTGATGCAGGCTGGCACGCATCCCGATTACTACACCCTGGCTCCCGAAAAAGGAAAAAATACGCTGGGCGTTGATGCGGTACGTGAGGTCACCGAAAAGCTGAATGAGCACGCACGCTTAGGTGGTGCGAAAGTCGTTTGGGTAACCGATGCTGCCTTACTAACCGACGCCGCGGCTAACGCATTGCTGAAAACGCTTGAAGAGCCACCAGCAGAAACTTGGTTTTTCCTGGCTACCCGCGAGCCTGAACGTTTACTGGCA  >  NC_000913/1155951‑1156220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A