Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A39 F60 I1 R1	17	70.9	2401242	98.9%	2374828	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,625,778	T→C	V295A (GTG→GCG)	serB →	phosphoserine phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,625,778	0	T	C	100.0%	208.6 / NA	54	V295A (GTG→GCG)	serB	phosphoserine phosphatase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (26/28); total (26/28)

TCTGACTCGCCTCGCGCAGGAGTATGAAATCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGT  >  NC_000913/4625650‑4625913

tctGACTCGCCTCGCGCAGGAGTATGAAATCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGCGAATGAAAAgg                                                                                                                              <  2:593375/140‑1 (MQ=255)

          ctcgcGCAGGAGTATGAAATCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCAc                                                                                                                    >  2:191217/1‑140 (MQ=255)

           tcgcGCAGGAGTATGAAATCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATACCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCAcc                                                                                                                   <  2:215563/140‑1 (MQ=255)

               gcAGGAGTATGAAATCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATcc                                                                                                               <  2:553642/140‑1 (MQ=255)

                cAGGAGTATGAAATCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCg                                                                                                              <  1:1052250/140‑1 (MQ=255)

                      tATGAAATCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGc                                                                                                        >  1:108324/1‑140 (MQ=255)

                        tGAAATCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTg                                                                                                      >  1:107032/1‑140 (MQ=255)

                        tGAAATCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTg                                                                                                      >  2:392685/1‑140 (MQ=255)

                          aaaTCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGAc                                                                                                    <  1:893906/140‑1 (MQ=255)

                             tCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTg                                                                                                 >  1:202576/1‑140 (MQ=255)

                             tCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTg                                                                                                 >  2:1039394/1‑140 (MQ=255)

                              cccGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGa                                                                                                <  2:984223/140‑1 (MQ=255)

                              cccGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGa                                                                                                <  1:392685/140‑1 (MQ=255)

                               ccGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGAt                                                                                               >  1:1075659/1‑140 (MQ=255)

                                  cTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGa                                                                                                <  1:470859/136‑1 (MQ=255)

                                  cTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGa                                                                                                >  2:470859/1‑136 (MQ=255)

                                   tGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATgggg                                                                                           <  2:252785/140‑1 (MQ=255)

                                           aCCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTg                                                                                   >  1:263389/1‑140 (MQ=255)

                                             cGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCa                                                                                 <  1:406253/140‑1 (MQ=255)

                                             cGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCa                                                                                 <  1:585236/140‑1 (MQ=255)

                                               tggcgattggcgatGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATc                                                                               >  1:1187844/1‑140 (MQ=255)

                                                ggcgattggcgatGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATcc                                                                              >  1:178209/1‑140 (MQ=255)

                                                 gcgattggcgatGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCct                                                                             >  2:301335/1‑140 (MQ=255)

                                                       ggcgatGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTTGGGATTGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAgg                                                                       <  2:605108/140‑1 (MQ=255)

                                                       ggcgatGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAgg                                                                       >  2:1050863/1‑140 (MQ=255)

                                                       ggcgatGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAgg                                                                       <  2:202576/140‑1 (MQ=255)

                                                       ggcgatGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAgg                                                                       <  2:263389/140‑1 (MQ=255)

                                                            tGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGcc                                                                  <  2:349023/140‑1 (MQ=255)

                                                            tGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGcc                                                                  <  1:1039394/140‑1 (MQ=255)

                                                               aGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGa                                                               <  2:164743/140‑1 (MQ=255)

                                                               aGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGGCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGa                                                               >  2:368477/1‑140 (MQ=255)

                                                                  cAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATc                                                            <  1:611308/140‑1 (MQ=255)

                                                                            ccGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAg                                                          <  2:62249/132‑1 (MQ=255)

                                                                            ccGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAg                                                          >  1:62249/1‑132 (MQ=255)

                                                                            ccGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAAtt                                                  >  2:953681/1‑140 (MQ=255)

                                                                                          ggCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATc                                    <  2:1187844/140‑1 (MQ=255)

                                                                                                      gATTGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGca                        <  1:691307/140‑1 (MQ=255)

                                                                                                         tGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGca                        <  1:20435/137‑1 (MQ=255)

                                                                                                         tGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGca                        >  2:20435/1‑137 (MQ=255)

                                                                                                         tGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAg                     >  1:152713/1‑140 (MQ=255)

                                                                                                         tGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAg                     >  1:883724/1‑140 (MQ=255)

                                                                                                         tGCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAg                     >  1:696104/1‑140 (MQ=255)

                                                                                                          gCCTACCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGc                    >  1:829652/1‑140 (MQ=255)

                                                                                                              aCCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGca                        <  1:1055451/132‑1 (MQ=255)

                                                                                                              aCCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGca                        >  2:589084/1‑132 (MQ=255)

                                                                                                              aCCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGca                        <  1:589084/132‑1 (MQ=255)

                                                                                                              aCCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGca                        >  2:1055451/1‑132 (MQ=255)

                                                                                                              aCCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTa                <  2:107032/140‑1 (MQ=255)

                                                                                                              aCCATGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTa                <  2:178209/140‑1 (MQ=255)

                                                                                                                 aTGCCAAGTCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACg             <  1:368477/140‑1 (MQ=255)

                                                                                                                 aTGCCAAGCCAAAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACg             <  1:301335/140‑1 (MQ=255)

                                                                                                                   gccaagccaaAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAg           >  2:742399/1‑140 (MQ=255)

                                                                                                                     caagccaaAAGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGt         >  1:964301/1‑140 (MQ=255)

                                                                                                                            aaaGCGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGt  >  1:923271/1‑140 (MQ=255)

TCTGACTCGCCTCGCGCAGGAGTATGAAATCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGT  >  NC_000913/4625650‑4625913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A