Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F60 I1 R1	24	41.1	1397072	98.8%	1380307	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,991,733	C→G	A194G (GCC→GGC)	cyaA →	adenylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,991,733	0	C	G	100.0%	162.2 / NA	42	A194G (GCC→GGC)	cyaA	adenylate cyclase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (15/27); total (15/27)

GTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGAC‑‑TATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGATCTCGGTGGCTTAAGCTCGCT  >  NC_000913/3991597‑3991865

gtgGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTcggcgg                                                                                                                                     <  2:571961/140‑1 (MQ=255)

  ggAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTa                                                                                                                                   <  2:377541/140‑1 (MQ=255)

     aGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGc                                                                                                                                <  2:53644/140‑1 (MQ=255)

     aGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGc                                                                                                                                <  2:626302/140‑1 (MQ=255)

       tCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGCGt                                                                                                                              >  1:67624/1‑140 (MQ=255)

       tCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGCGt                                                                                                                              >  1:38142/1‑140 (MQ=255)

       tCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGCGt                                                                                                                              >  1:392506/1‑140 (MQ=255)

       tCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGCGt                                                                                                                              >  2:59486/1‑140 (MQ=255)

         aGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGCGTAt                                                                                                                            >  2:438287/1‑140 (MQ=255)

               ttcCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGCGTATTCtgtg                                                                                                                      <  2:67624/140‑1 (MQ=255)

               ttcCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGCGTATTCtgtg                                                                                                                      <  1:614371/140‑1 (MQ=255)

               ttcCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGCGTATTCtgtg                                                                                                                      <  1:532934/140‑1 (MQ=255)

                 cgtgattgatGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGCGTATTCTGTGGa                                                                                                                    <  2:127813/138‑1 (MQ=255)

                    gattgatGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGCGTATTCTGTGGAata                                                                                                                 <  2:38142/140‑1 (MQ=255)

                     attgatGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGc                                                                                                                                >  1:664945/1‑124 (MQ=255)

                     attgatGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGc                                                                                                                                <  2:664945/124‑1 (MQ=255)

                              aaCCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGCGTATTCTGTGGAATATGGTGCCGTg                                                                                                       <  2:297954/140‑1 (MQ=255)

                              aaCCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGCGTATTCTGTGGAATATGGTGCCGTg                                                                                                       <  1:644438/140‑1 (MQ=255)

                              aaCCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGCGTATTCTGTGGAATATGGTGCCGTg                                                                                                       <  1:202114/140‑1 (MQ=255)

                                 cGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGcga                                                                                                    <  1:52066/140‑1 (MQ=255)

                                 cGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGcga                                                                                                    <  1:668846/140‑1 (MQ=255)

                                    ttCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGcgacga                                                                                                 >  1:405602/1‑140 (MQ=255)

                                        gTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACgaagaa                                                                                             <  1:256965/140‑1 (MQ=255)

                                            tAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGc                                                                                         <  1:468377/140‑1 (MQ=255)

                                                 aaaGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTac                                                                                    <  1:683215/140‑1 (MQ=255)

                                                   aGCGGCAGCCTGGGGGGCGAGGATTGTGGCTCTACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTacga                                                                                  <  2:405602/140‑1 (MQ=255)

                                                   aGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTacga                                                                                  <  2:657574/140‑1 (MQ=255)

                                                    gCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTacgac                                                                                 <  1:1818/140‑1 (MQ=255)

                                                      ggCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACTAAGAAGAGCATTAcgacga                                                                               <  1:543726/140‑1 (MQ=255)

                                                      ggCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTAcgacga                                                                               <  1:641855/140‑1 (MQ=255)

                                                       gCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACgacgac                                                                              >  1:136831/1‑140 (MQ=255)

                                                                  ggCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGCGTATTCTGGGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGAC‑‑TATGTGATGAc                                                                 >  1:2470/1‑140 (MQ=255)

                                                                     gAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGAC‑‑TATGTGATGACGCt                                                              >  2:96445/1‑140 (MQ=255)

                                                                            gtgGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGAC‑‑TATGTGATGACGCTTTAcgcg                                                       <  1:438287/140‑1 (MQ=255)

                                                                                 tCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGAC‑‑TATGTGATGACGCTTTACGCGCAggg                                                  >  2:626438/1‑140 (MQ=255)

                                                                                     cccAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGAC‑‑TATGTGATGACGCTTTACGCGCAGGGCGTg                                              >  1:619844/1‑140 (MQ=255)

                                                                                       cAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGAC‑‑TATGTGATGACGCTTTACGCGCAGGGCGTGCt                                            <  2:92493/140‑1 (MQ=255)

                                                                                                   ctgctTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGAC‑‑TATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAaatga                                >  1:107518/1‑140 (MQ=255)

                                                                                                    tgctTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGAC‑‑TATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAaatgaa                               <  1:96445/140‑1 (MQ=255)

                                                                                                      ctTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGAC‑‑TATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAaatga                                <  2:577274/137‑1 (MQ=255)

                                                                                                      ctTGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGAC‑‑TATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAaatga                                >  1:577274/1‑137 (MQ=255)

                                                                                                        tGACGAATTTTATCGTACCGCCGTGCGTCTCGGCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAaatgaatg                             >  1:375211/1‑140 (MQ=255)

                                                                                                                                   tctcGGCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGACTATATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGATCTCGGTGGCTTAAGCTCGCt  <  2:375211/140‑1 (MQ=255)

GTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGACGAC‑‑TATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGATCTCGGTGGCTTAAGCTCGCT  >  NC_000913/3991597‑3991865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A