Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F60 I1 R1	21	41.4	1402642	98.7%	1384407	138.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,077,284	C→G	R90R (CGC→CGG)	dtd →	D‑aminoacyl‑tRNA deacylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,077,284	0	C	G	100.0%	178.2 / NA	47	R90R (CGC→CGG)	dtd	D‑aminoacyl‑tRNA deacylase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (23/24); total (23/24)

GCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTATATGACTATTTCGTCGAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTC  >  NC_000913/4077151‑4077416

gCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGGCCAAGt                                                                                                                                <  1:563453/140‑1 (MQ=255)

   aGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGGCCAAGTTtc                                                                                                                             >  1:542312/1‑140 (MQ=255)

     cGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGGCCAAGTTtctc                                                                                                                           >  2:660335/1‑140 (MQ=255)

     cGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGGCCAAGTTtctc                                                                                                                           >  1:56360/1‑140 (MQ=255)

         tgCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGGCCAAGTTTCTCCaaa                                                                                                                       >  2:144431/1‑140 (MQ=255)

         tgCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGGCCAAGTTTCTCCaaa                                                                                                                       <  2:122970/140‑1 (MQ=255)

             cGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGGCCAAGTTTTTCCAAAGGTg                                                                                                                   <  1:537698/140‑1 (MQ=255)

             cGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGGCCAAGTTTCTCCAAAGGTg                                                                                                                   <  2:87238/140‑1 (MQ=255)

             cGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGGCCAAGTTTCTCCAAAGGTg                                                                                                                   <  2:542312/140‑1 (MQ=255)

             cGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGGCCAAGTTTCTCCAAAGGTg                                                                                                                   <  2:242594/140‑1 (MQ=255)

                   ccGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGGCCAAGTTTCTCCAAAGGTGCATCAc                                                                                                             >  1:355262/1‑140 (MQ=255)

                      cATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGGCCAAGTTTCTCCAAAGGTGCATCACCgg                                                                                                          >  2:493017/1‑140 (MQ=255)

                             aGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGTGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGGCCAAGTTTCTCCAAAGGTg                                                                                                                   >  1:312365/1‑124 (MQ=255)

                             aGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGTGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGGCCAAGTTTCTCCAAAGGTg                                                                                                                   <  2:312365/124‑1 (MQ=255)

                               cGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGGCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCag                                                                                                 <  1:125484/140‑1 (MQ=255)

                                  tGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGGCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAgg                                                                                              <  1:544361/140‑1 (MQ=255)

                                  tGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGGCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAgg                                                                                              >  1:497757/1‑140 (MQ=255)

                                  tGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGGCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAgg                                                                                              <  1:660335/140‑1 (MQ=255)

                                  tGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGGCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAgg                                                                                              <  2:497757/140‑1 (MQ=255)

                                  tGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGGCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAgg                                                                                              <  2:451484/140‑1 (MQ=255)

                                   gCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGGCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGc                                                                                             >  1:678479/1‑140 (MQ=255)

                                     cGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGGCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGt                                                                                           <  2:56360/140‑1 (MQ=255)

                                     cGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGGCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGt                                                                                           <  1:388277/140‑1 (MQ=255)

                                       aaGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGGCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTta                                                                                         <  2:355262/140‑1 (MQ=255)

                                         ggCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGGCCAAGTTTCTCAAAAGGTGCATCACCGGATCGCGCAGAGGCGTtata                                                                                       >  1:145091/1‑140 (MQ=255)

                                            aaGATGAATTTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGGCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCag                                                                                                 >  2:462763/1‑127 (MQ=255)

                                            aaGATGAATTTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGGCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCag                                                                                                 <  1:462763/127‑1 (MQ=255)

                                                    tctcAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGGCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGt                                                                                           >  2:320564/1‑125 (MQ=255)

                                                    tctcAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGGCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGt                                                                                           <  1:320564/125‑1 (MQ=255)

                                                    tctcAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGGCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTATATGACTATTtcg                                                                            >  1:522459/1‑140 (MQ=255)

                                                    tctcAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGGCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTATATGACTATTtcg                                                                            >  2:117506/1‑140 (MQ=255)

                                                        aaCGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGGCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTATATGACTATTTCGTCGa                                                                        <  1:493017/140‑1 (MQ=255)

                                                                           gcAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGGCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTAAATGACTATTTCGTCGAACGCTGCCGTCAGCAagag                                                     <  1:144431/140‑1 (MQ=255)

                                                                                gtgCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGGCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTATATGACTATTTCGTCGAACGCTGCCGTCAGCAAGAGATGaa                                                <  1:117506/140‑1 (MQ=255)

                                                                                                    tACCCTCGCCGCAGATACCGAACGGGGGATGCGGCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTATATGACTATTTCGTCGAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCg                            >  1:634896/1‑140 (MQ=255)

                                                                                                     aCCCTCGCCGCAGATACCGAACGGGGGATGCGGCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTATATGACTATTTCGTCGAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGc                           <  2:678479/140‑1 (MQ=255)

                                                                                                       ccTCGCCGCAGATACCGAACGGGGGATGCGGCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTATATGACTATTTCGTCGAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTg                         >  1:66334/1‑140 (MQ=255)

                                                                                                           gccgcAGATACCGAACGGGGGATGCGGCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTATATGACTATTTCGTCGAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCg                            <  1:257783/133‑1 (MQ=255)

                                                                                                           gccgcAGATACCGAACGGGGGATGCGGCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTATATGACTATTTCGTCGAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCg                            >  2:257783/1‑133 (MQ=255)

                                                                                                           gccgcAGATACCGAACGGGGGATGCGGCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTATATGACTATTTCGTCGAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGa                     >  2:569302/1‑140 (MQ=255)

                                                                                                           gccgcAGATACCGAACGGGGGATGCGGCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTATATGACTATTTCGTCGAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGa                     >  2:207509/1‑140 (MQ=255)

                                                                                                            ccgcAGATACCGAACGGGGGATGCGGCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTATATGACTATTTCGTCGAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGat                    >  2:605135/1‑140 (MQ=255)

                                                                                                                 gATACCGAACGGGGGATGCGGCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTATATGACTATTTCGTCGAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCa               <  2:66334/140‑1 (MQ=255)

                                                                                                                       gAACGGGGGATGCGGCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTATATGACTATTTCGTCGAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATc         >  2:665051/1‑140 (MQ=255)

                                                                                                                            ggggATGCGGCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTATATGACTATTTCGTCGAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTgg    >  2:175004/1‑140 (MQ=255)

                                                                                                                                aTGCGGCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTATATGACTATTTCGTCGAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTc  <  2:386006/138‑1 (MQ=255)

                                                                                                                                aTGCGGCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTATATGACTATTTCGTCGAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTc  >  1:386006/1‑138 (MQ=255)

GCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGTTATATGACTATTTCGTCGAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTC  >  NC_000913/4077151‑4077416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A