Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F60 I1 R1	21	40.1	1360994	98.8%	1344662	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	950,590	A→C	V164G (GTG→GGG)	pflA ←	pyruvate formate‑lyase activating enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	950,590	0	A	C	100.0%	179.9 / NA	49	V164G (GTG→GGG)	pflA	pyruvate formate‑lyase activating enzyme
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (20/29); total (20/29)

ATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAAGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACACCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTTGGTGGATCTCGTCGTTCATCTGTTTGAGATCGAGCATTACCAGGTCGGTTACTTCCAG  >  NC_000913/950453‑950720

aTTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAAGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATC‑TCAGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCt                                                                                                                                 <  1:46612/140‑1 (MQ=255)

 tttGTGTTTGCCCAGCTCGTGGTAGGGGAGAAGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCt                                                                                                                                 <  1:157389/140‑1 (MQ=255)

 tttGTGTTTGCCCAGCTCGTGGTAGGGGAGAAGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCt                                                                                                                                 <  1:631918/140‑1 (MQ=255)

 tttGTGTTTGCCCAGCTCGTGGTAGGGGAGAAGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCt                                                                                                                                 <  1:612551/140‑1 (MQ=255)

 tttGTGTTTGCCCAGCTCGTGGTAGGGGAGAAGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCt                                                                                                                                 <  1:536929/140‑1 (MQ=255)

       tttGCCCAGCTCGTGGTAGGGGAGAAGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACAt                                                                                                                           >  2:590050/1‑140 (MQ=255)

        ttGCCCAGCTCGTGGTAGGGGAGAAGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACAtt                                                                                                                          >  2:52101/1‑140 (MQ=255)

         tGCCCAGCTCGTGGTA‑GGGAGAAGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACAtttt                                                                                                                        >  1:533286/1‑140 (MQ=255)

            ccAGCTCGTGGTAGGGGAGAAGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACATTTTTg                                                                                                                      <  1:542105/140‑1 (MQ=255)

            ccAGCTCGTGGTAGGGGAGAAGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACATTTTTg                                                                                                                      <  2:587725/140‑1 (MQ=255)

             cAGCTCGTGGTAGGGGAGAAGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACATTTTTGt                                                                                                                     <  2:182774/140‑1 (MQ=255)

                 tCGTGGTAGGGGAGAAGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACATTTTTGTTCGc                                                                                                                 <  2:376992/140‑1 (MQ=255)

                          gggAGAAGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACATTTTTGTTCGCCAGATAttt                                                                                                        >  2:571345/1‑140 (MQ=255)

                              gaAGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACATTTTTGTTCGCCAGATATTTAGCg                                                                                                    <  1:463522/140‑1 (MQ=255)

                               aaGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACATTTTTGTTCGCCAGATATTTAGCGa                                                                                                   >  2:308032/1‑140 (MQ=255)

                               aaGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACATTTTTGTTCGCCAGATATTTAGCGa                                                                                                   >  1:386110/1‑140 (MQ=255)

                               aaGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACATTTTTGTTCGCCAGATATTTAGCGa                                                                                                   >  1:528104/1‑140 (MQ=255)

                                     gATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCa                                                                                             <  1:231328/140‑1 (MQ=255)

                                     gATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCa                                                                                             <  1:586455/140‑1 (MQ=255)

                                     gATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCa                                                                                             <  1:188887/140‑1 (MQ=255)

                                         ttCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTAATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGt                                                                                         <  2:354756/140‑1 (MQ=255)

                                                    gCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTgg                                                                              >  2:421460/1‑140 (MQ=255)

                                                     cccATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGa                                                                             >  2:651834/1‑140 (MQ=255)

                                                      ccATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGaa                                                                            <  1:171120/140‑1 (MQ=255)

                                                             aCGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCaa                                                                     <  2:533286/140‑1 (MQ=255)

                                                               gggTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACATTTTTGTTCGCCAGATATTTAGTGAACTCCAGCGTGCGGTGGTTGGAAACTCCAAcc                                                                   >  2:498835/1‑140 (MQ=255)

                                                               gggTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAAcc                                                                   >  1:153636/1‑140 (MQ=255)

                                                               gggTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAAcc                                                                   >  2:199430/1‑140 (MQ=255)

                                                                       tCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTtg                                                           >  1:484077/1‑140 (MQ=255)

                                                                         aCCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTtggt                                                         <  1:221089/140‑1 (MQ=255)

                                                                           cGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTtggtgg                                                       >  2:34469/1‑140 (MQ=255)

                                                                              ggCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACATTCTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTTGGTGGAtc                                                    >  1:338269/1‑140 (MQ=255)

                                                                               gCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACATTTTTGTTCGCCAGATATTTAGTGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTTGGTGGAtct                                                   <  1:498835/140‑1 (MQ=255)

                                                                               gCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTTGGTGGAtct                                                   <  1:52101/140‑1 (MQ=255)

                                                                                         gAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTTGGTGGATCTCGTCGTTCAt                                         <  2:606643/140‑1 (MQ=255)

                                                                                            tcgtcatcgtcaGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTTGGTGGATCTCGTCGTTCATCTg                                      <  2:528104/140‑1 (MQ=255)

                                                                                             cgtcatcgtcaGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTTGGTGGATCTCGTCGTTCATCTGt                                     <  1:652650/140‑1 (MQ=255)

                                                                                                catcgtcaGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTCTGGTGGATCTCGTCGTTCATCTGTTTg                                  <  1:308032/140‑1 (MQ=255)

                                                                                                    gtcaGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTTGGTGGATCTCGTCGTTCATCTGTTTGAGAt                              >  2:498735/1‑140 (MQ=255)

                                                                                                    gtcaGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTTGGTGGATCTCGTCGTTCATCTGTTTGAGAt                              >  2:317339/1‑140 (MQ=255)

                                                                                                    gtcaGACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTTGGTGGATCTCGTCGTTCATCTGTTTGAGAt                              >  1:617835/1‑140 (MQ=255)

                                                                                                        gACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTTGGTGGATCTCGTCGTTCATCTGTTTGAGATCGAg                          <  2:386110/140‑1 (MQ=255)

                                                                                                        gACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTTGGTGGATCTCGTCGTTCATCTGTTTGAGATCGAg                          <  1:571345/140‑1 (MQ=255)

                                                                                                        gACCAGCCTGGGACAACAACGTAGCGGATCCACCCCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTTGGTGGATCTCGTCGTTCATCTGTTTGAGATCGAg                          <  1:184590/140‑1 (MQ=255)

                                                                                                                   gACAACAACGTAGCGGATCCACCCCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTTGGTGGATCTCGTCGTTCATCTGTTTGAGATCGAGCATTACCAGGt               <  1:554551/140‑1 (MQ=255)

                                                                                                                        caacGTAGCGGATCCACCCCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTTGGTGGATCTCGTCGTTCATCTGTTTGAGATCGAGCATTACCAGGTCGGtt          >  1:50699/1‑140 (MQ=255)

                                                                                                                        caacGTAGCGGATCCACCCCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTTGGTGGATCTCGTCGTTCATCTGTTTGAGATCGAGCATTACCAGGTCGGtt          >  2:97123/1‑140 (MQ=255)

                                                                                                                                cGGATCCACCCCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGTGTGCGGTGGTTGGAAACTCCAACCAGATTTTGGTGGATCTCGTCGTTCATCTGTTTGAGATCGAGCATTACCAGGTCGGTTACTTCcag  <  2:307795/140‑1 (MQ=255)

                                                                                                                                cGGATCCACCCCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTTGGTGGATCTCGTCGTTCATCTGTTTGAGATCGAGCATTACCAGGTCGGTTACTTCcag  <  1:34469/140‑1 (MQ=255)

ATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAAGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACACCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTTGGTGGATCTCGTCGTTCATCTGTTTGAGATCGAGCATTACCAGGTCGGTTACTTCCAG  >  NC_000913/950453‑950720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A