Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F28 I1 R1 140 77.1 2730754 94.9% 2591485 138.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,464,788 T→C G106G (GGT→GGC)  paaY → 2‑hydroxycyclohepta‑1,4,6‑triene‑1‑carboxyl‑CoA thioesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,464,7880TC98.5% 223.4 / ‑4.4 65G106G (GGT→GGC) paaY2‑hydroxycyclohepta‑1,4,6‑triene‑1‑carboxyl‑CoA thioesterase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (42/22);  total (42/23)
Fisher's exact test for biased strand distribution p-value = 3.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

ATTGCGTTATGCACGGTTTTCCCGAGCAGGATACTGTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAGTGAGCAGGAGTTGGCATG  >  NC_000913/1464652‑1464922
                                                                                                                                        |                                                                                                                                      
aTTGCGTTATGCACGGTTTTCCCGAGCAGGATACTGTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGAcggcgcg                                                                                                                                     >  1:1182956/1‑140 (MQ=255)
 ttGCGTTATGCACGGTTTTCCCGAGCAGGATACTGTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGAcggcgcgg                                                                                                                                    >  2:207818/1‑140 (MQ=255)
      ttATGCACGGTTTTCCCGAGCAGGATACTGTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGAtt                                                                                                                               <  1:1269766/140‑1 (MQ=255)
       tATGCACGGTTTTCCCGAGCAGGATACTGTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTg                                                                                                                              <  1:207818/140‑1 (MQ=255)
         tGCACGGTTTTCCCGAGCAGGATACTGTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGc                                                                                                                            >  2:520315/1‑140 (MQ=255)
         tGCACGGTTTTCCCGAGCAGGATACTGTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGc                                                                                                                            >  1:645875/1‑140 (MQ=255)
           cACGGTTTTCCCGAGCAGGATACTGTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGa                                                                                                                                 >  2:1286636/1‑133 (MQ=255)
           cACGGTTTTCCCGAGCAGGATACTGTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGa                                                                                                                                 <  1:1286636/133‑1 (MQ=255)
           cACGGTTTTCCCGAGCAGGATACTGTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCga                                                                                                                          >  1:51801/1‑140 (MQ=255)
              ggTTTTCCCGAGCAGGATACTGTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGaa                                                                                                                       >  1:1062345/1‑140 (MQ=255)
              ggTTTTCCCGAGCAGGATACTGTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGaa                                                                                                                       <  2:1182956/140‑1 (MQ=255)
                 tttCCCGAGCAGGATACTGTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAg                                                                                                                    <  1:950301/140‑1 (MQ=255)
                  ttCCCGAGCAGGATACTGTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGc                                                                                                                   <  2:1225167/140‑1 (MQ=255)
                   tCCCGAGCAGGATACTGTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCa                                                                                                                  <  2:562447/140‑1 (MQ=255)
                   tCCCGAGCAGGATACTATTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCa                                                                                                                  >  1:799343/1‑140 (MQ=255)
                          cAGGATACTGTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTgg                                                                                                           >  1:481738/1‑140 (MQ=255)
                           aGGATACTGTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGt                                                                                                          <  1:725756/140‑1 (MQ=255)
                                     tgtAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCAt                                                                                                <  1:1045511/140‑1 (MQ=255)
                                       tAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCAttt                                                                                              >  2:1324757/1‑140 (MQ=255)
                                       tAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCAttt                                                                                              >  1:298802/1‑140 (MQ=255)
                                       tAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCAttt                                                                                              >  1:749745/1‑140 (MQ=255)
                                       tAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCAttt                                                                                              >  1:139801/1‑140 (MQ=255)
                                              agaTGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAg                                                                                       >  1:1283716/1‑140 (MQ=255)
                                                aTGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGcc                                                                                     >  1:1084632/1‑140 (MQ=255)
                                                     cATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGc                                                                                >  1:594907/1‑140 (MQ=255)
                                                         ttGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGaa                                                                            >  1:1116106/1‑140 (MQ=255)
                                                          tGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGaaa                                                                           >  1:865651/1‑140 (MQ=255)
                                                             tCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGc                                                                        <  1:1324757/140‑1 (MQ=255)
                                                              cATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGcc                                                                       <  1:520315/140‑1 (MQ=255)
                                                              cATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGcc                                                                       <  2:285015/140‑1 (MQ=255)
                                                              cATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTCAAAGCCAAAGCAGAAATGcc                                                                       <  2:1062345/140‑1 (MQ=255)
                                                               aTAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCa                                                                      >  2:155608/1‑140 (MQ=255)
                                                                tAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAg                                                                              >  1:750807/1‑131 (MQ=255)
                                                                tAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAg                                                                              <  2:750807/131‑1 (MQ=255)
                                                                 aGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGc                                                                    >  2:1173407/1‑140 (MQ=255)
                                                                  gcgcTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCt                                                                   <  1:155608/140‑1 (MQ=255)
                                                                   cgcTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAg                                                                     >  1:166041/1‑137 (MQ=255)
                                                                   cgcTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAg                                                                     <  2:166041/137‑1 (MQ=255)
                                                                      tATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAAtt                                                               >  2:442567/1‑140 (MQ=255)
                                                                         ccTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTAcc                                                            <  2:139801/140‑1 (MQ=255)
                                                                                  cTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCg                                                   >  1:501845/1‑140 (MQ=255)
                                                                                  cTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCg                                                   >  1:481324/1‑140 (MQ=255)
                                                                                    gCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGc                                                 >  1:574089/1‑140 (MQ=255)
                                                                                     cATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCa                                                >  2:482140/1‑140 (MQ=255)
                                                                                               cgcAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGaa                                      >  1:482185/1‑140 (MQ=255)
                                                                                                    tGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGa                                 >  1:458344/1‑140 (MQ=255)
                                                                                                    tGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGa                                 >  2:493836/1‑140 (MQ=255)
                                                                                                        ttAGTGGGAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCg                             <  2:1084632/140‑1 (MQ=255)
                                                                                                              ggAATGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACt                       <  2:501845/140‑1 (MQ=255)
                                                                                                                aaTGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCa                     >  1:1182557/1‑140 (MQ=255)
                                                                                                                aaTGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCa                     <  2:645875/140‑1 (MQ=255)
                                                                                                                aaTGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCa                     >  1:1014148/1‑140 (MQ=255)
                                                                                                                 aTGAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAg                    >  2:1113543/1‑140 (MQ=255)
                                                                                                                   gAACGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAGTg                  >  1:945953/1‑140 (MQ=255)
                                                                                                                     aCGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGa                                 >  2:391600/1‑123 (MQ=255)
                                                                                                                     aCGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGa                                 <  1:391600/123‑1 (MQ=255)
                                                                                                                     aCGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAGTGAg                <  2:749745/140‑1 (MQ=255)
                                                                                                                     aCGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAGTGAg                >  1:753655/1‑140 (MQ=255)
                                                                                                                     aCGCGGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAGTGAg                >  1:1365135/1‑140 (MQ=255)
                                                                                                                      cgcgGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAGTGAGc               >  2:86970/1‑140 (MQ=255)
                                                                                                                      cgcgGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAGTGAGc               >  2:884804/1‑140 (MQ=255)
                                                                                                                      cgcgGTAGTGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAGTGAGc               >  2:635794/1‑140 (MQ=255)
                                                                                                                              tGATGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAGTGAGCAGGAGTTg       >  2:227763/1‑140 (MQ=255)
                                                                                                                                aTGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAGTGAGCAGGAGTTGGc     >  2:968313/1‑140 (MQ=255)
                                                                                                                                aTGGACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAGTGAGCAGGAGTTGGc     <  1:635794/140‑1 (MQ=255)
                                                                                                                                   gACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAGTGAGCAGGAGTTGGCATg  >  1:156074/1‑140 (MQ=255)
                                                                                                                                   gACGGCGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAGTGAGCAGGAGTTGGCATg  >  1:940747/1‑140 (MQ=255)
                                                                                                                                     cggcgcggtgATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAGTGAGc               <  2:157650/125‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
ATTGCGTTATGCACGGTTTTCCCGAGCAGGATACTGTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAGTGAGCAGGAGTTGGCATG  >  NC_000913/1464652‑1464922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A