Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	132	43.0	1464274	99.1%	1451095	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,220,971	G→A	pseudogene (1371/2648 nt)	ycgH →	putative transporter component, N‑terminal fragment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,220,971	0	G	A	100.0%	194.2 / NA	54	pseudogene (1371/2648 nt)	ycgH	putative transporter component, N‑terminal fragment
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (32/22); total (32/22)

AGACAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCGGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGAATCTCCAGCA  >  NC_000913/1220839‑1221106

aGACAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACgg                                                                                                                                  <  1:628687/140‑1 (MQ=255)

aGACAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACgg                                                                                                                                  <  1:623319/140‑1 (MQ=255)

  aCAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGAc                                                                                                                                >  1:47644/1‑140 (MQ=255)

      tattatCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGAc                                                                                                                            >  1:183247/1‑140 (MQ=255)

      tattatCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGAc                                                                                                                            >  2:613331/1‑140 (MQ=255)

      tattatCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGAc                                                                                                                            >  1:511771/1‑140 (MQ=255)

                cTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGa                                                                                                                  >  2:400873/1‑140 (MQ=255)

                cTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGa                                                                                                                  <  2:520118/140‑1 (MQ=255)

                cTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGa                                                                                                                  >  1:520118/1‑140 (MQ=255)

                    cGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGt                                                                                                              <  2:368577/140‑1 (MQ=255)

                         gatgatgGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATAc                                                                                                         >  1:85979/1‑140 (MQ=255)

                           tgatgGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATGATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACtt                                                                                                       <  1:395442/140‑1 (MQ=255)

                           tgatgGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACtt                                                                                                       <  1:400873/140‑1 (MQ=255)

                               ggTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCggg                                                                                                   <  1:613331/140‑1 (MQ=255)

                                gTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGt                                                                                                  >  2:675828/1‑140 (MQ=255)

                                 tCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGtt                                                                                                 <  2:85979/140‑1 (MQ=255)

                                  cAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTc                                                                                                <  1:675828/140‑1 (MQ=255)

                                       aTTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTa                                                                                           <  1:382375/140‑1 (MQ=255)

                                          tACCCAA‑GAAAAACATTAACCGTAAAAGGGAATTATGGCGGGAATAAATGTCAACTTAATATTCGCAACGGATTAAGGGATGATAAATCAACTAAGGACCAACTTATTGGTTAGGGGAATACTTCGGGGTCAACTACCgg                                                                                       >  2:384190/1‑139 (MQ=255)

                                           aCCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGt                                                                                       >  1:389636/1‑140 (MQ=255)

                                           aCCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGt                                                                                       >  1:272964/1‑140 (MQ=255)

                                                 ggAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTAtgt                                                                                 >  2:135736/1‑140 (MQ=255)

                                                                        tAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAgcggcg                                                          <  2:511771/140‑1 (MQ=255)

                                                                         aaTTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAgcggcgc                                                         >  2:609619/1‑140 (MQ=255)

                                                                           ttATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAgcggcgcgg                                                       <  1:276476/140‑1 (MQ=255)

                                                                            tATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAgcggcgcggc                                                      <  2:47644/140‑1 (MQ=255)

                                                                            tATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAgcggcgcggc                                                      <  1:609619/140‑1 (MQ=255)

                                                                              tGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCa                                                    >  2:470621/1‑140 (MQ=255)

                                                                                 cGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGc                                                 >  1:458870/1‑140 (MQ=255)

                                                                                          tGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGa                                        >  2:614887/1‑140 (MQ=255)

                                                                                              cAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGa                                    <  2:389636/140‑1 (MQ=255)

                                                                                               aGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATc                                      <  2:81213/137‑1 (MQ=255)

                                                                                               aGCTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATc                                      >  1:81213/1‑137 (MQ=255)

                                                                                                 cTTAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACt                                 >  1:474172/1‑140 (MQ=255)

                                                                                                   tAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCGCGCTAAACGGGATCGAACTCa                               <  2:458870/140‑1 (MQ=255)

                                                                                                   tAATATCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCa                               <  2:474172/140‑1 (MQ=255)

                                                                                                     atatCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATa                             <  1:470621/140‑1 (MQ=255)

                                                                                                     atatCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATa                             <  1:614887/140‑1 (MQ=255)

                                                                                                      tatCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATaa                            >  2:14539/1‑140 (MQ=255)

                                                                                                      tatCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATaa                            >  1:123261/1‑140 (MQ=255)

                                                                                                      tatCCGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATaa                            <  1:63239/140‑1 (MQ=255)

                                                                                                         ccGCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACtg                         >  1:603257/1‑140 (MQ=255)

                                                                                                           gCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACtgtg                       >  2:29846/1‑140 (MQ=255)

                                                                                                           gCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACtgtg                       >  2:505766/1‑140 (MQ=255)

                                                                                                           gCACCGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACtgtg                       >  2:571413/1‑140 (MQ=255)

                                                                                                                         gatgatAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGAAtc         >  2:350745/1‑140 (MQ=255)

                                                                                                                           tgatAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGAAtctc       >  1:612745/1‑140 (MQ=255)

                                                                                                                           tgatAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGAAtctc       >  1:482255/1‑140 (MQ=255)

                                                                                                                           tgatAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGAAtctc       >  1:690537/1‑140 (MQ=255)

                                                                                                                           tgatAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGAAtctc       >  2:188217/1‑140 (MQ=255)

                                                                                                                           tgatAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGAAtctc       >  2:83209/1‑140 (MQ=255)

                                                                                                                            gatAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGAATCTcc      >  2:676565/1‑140 (MQ=255)

                                                                                                                            gatAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGAATCTcc      >  1:136245/1‑140 (MQ=255)

                                                                                                                             atAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGa            <  2:576462/133‑1 (MQ=255)

                                                                                                                             atAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGa            >  1:576462/1‑133 (MQ=255)

                                                                                                                                aaTCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGAATCTCcagca  >  1:635620/1‑140 (MQ=255)

AGACAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTATTAGGTGATGATAAATCGGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGAATCTCCAGCA  >  NC_000913/1220839‑1221106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A