Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	142	96.2	3256224	98.9%	3220405	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,361,497	T→C	G126G (GGT→GGC)	puuD →	gamma‑glutamyl‑gamma‑aminobutyrate hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,361,497	0	T	C	97.9%	335.0 / ‑6.3	96	G126G (GGT→GGC)	puuD	gamma‑glutamyl‑gamma‑aminobutyrate hydrolase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (49/45); minor base G (1/1); total (50/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAACGGCGATGAGCCTGACGCCGATCCCGGGCGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGTTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAGGTTGAAGAGGGGGGATTACTGTCTGCGTTGT  >  NC_000913/1361361‑1361630

aaaaCGGCGATGAGCCTGACGCCGATCCCGGGCGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCg                                                                                                                                    >  1:562813/1‑140 (MQ=255)

aaaaCGGCGATGAGCCTGACGCCGATCCCGGGCGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCg                                                                                                                                    >  2:477395/1‑140 (MQ=255)

  aaCGGCGATGAGCCTGACGCCGATCCCGGGCGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGtt                                                                                                                                  >  1:1012004/1‑140 (MQ=255)

   aCGGCGATGAGCCTGACGCCGATCCCGGGCGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTg                                                                                                                                 >  1:1092639/1‑140 (MQ=255)

       cGATGAGCCTGACGCCGATCCCGGGCGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGGTCGTTGCATc                                                                                                                             >  1:1206872/1‑140 (MQ=255)

         aTGAGCCTGACGCCGATCCCGGGCGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGc                                                                                                                           <  1:477395/140‑1 (MQ=255)

         aTGAGCCTGACGCCGATCCCGGGCGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGc                                                                                                                           <  1:189180/140‑1 (MQ=255)

           gAGCCTGACGCCGATCCCGGGCGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCaa                                                                                                                         <  2:262698/140‑1 (MQ=255)

           gAGCCTGACGCCGATCCCGGGCGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCaa                                                                                                                         <  1:280091/140‑1 (MQ=255)

            aGCCTGACGCCGATCCCGGGCGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAg                                                                                                                        <  1:13867/140‑1 (MQ=255)

             gCCTGACGCCGATCCCGGGCGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGc                                                                                                                       <  1:581077/140‑1 (MQ=255)

               cTGACGCCGATCCCGGGCGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCtg                                                                                                                     >  2:157476/1‑140 (MQ=255)

                   cGCCGATCCCGGGCGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCg                                                                                                                 <  2:430155/140‑1 (MQ=255)

                     ccGATCCCGGGCGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGaa                                                                                                               <  1:965968/140‑1 (MQ=255)

                      cGATCCCGGGCGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGGTCGTTGCATCGCAAGCTGTGCGAAc                                                                                                              <  2:1206872/140‑1 (MQ=255)

                      cGATCCCGGGCGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAAc                                                                                                              <  1:372286/140‑1 (MQ=255)

                              ggCGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGaa                                                                                                      >  2:632764/1‑140 (MQ=255)

                                  tGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGc                                                                                                  >  2:614812/1‑140 (MQ=255)

                                  tGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGc                                                                                                  >  1:858831/1‑140 (MQ=255)

                                  tGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGc                                                                                                  >  1:1126632/1‑140 (MQ=255)

                                    aTCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTg                                                                                                >  2:1524381/1‑140 (MQ=255)

                                    aTCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTg                                                                                                >  1:240352/1‑140 (MQ=255)

                                      cttctGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGa                                                                                              <  2:858831/140‑1 (MQ=255)

                                      cttctGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGa                                                                                              <  2:240352/140‑1 (MQ=255)

                                      cttctGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGa                                                                                              <  1:429953/140‑1 (MQ=255)

                                          tGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACAt                                                                                          >  2:517770/1‑140 (MQ=255)

                                               aTGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGa                                                                                     <  2:959071/140‑1 (MQ=255)

                                                 ggCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAg                                                                                   >  2:1367286/1‑140 (MQ=255)

                                                 ggCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAg                                                                                   >  2:633112/1‑140 (MQ=255)

                                                 ggCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAg                                                                                   >  1:662046/1‑140 (MQ=255)

                                                 ggCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAg                                                                                   >  1:1041999/1‑140 (MQ=255)

                                                 ggCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAg                                                                                   >  1:906045/1‑140 (MQ=255)

                                                   cGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGTCTGAATTGCTGGAACATCGGGAAGAt                                                                                 >  1:34961/1‑140 (MQ=255)

                                                   cGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGAt                                                                                 >  2:372426/1‑140 (MQ=255)

                                                      taattaatGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATccc                                                                              >  2:249972/1‑140 (MQ=255)

                                                           aatGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACt                                                                         <  2:1041999/140‑1 (MQ=255)

                                                           aatGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACt                                                                         <  2:906045/140‑1 (MQ=255)

                                                              gCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGcc                                                                      <  1:408568/140‑1 (MQ=255)

                                                                  cACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTg                                                                  >  1:1168538/1‑140 (MQ=255)

                                                                  cACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTg                                                                  <  2:644081/140‑1 (MQ=255)

                                                                   aCTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTgg                                                                 <  2:562813/140‑1 (MQ=255)

                                                                   aCTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTgg                                                                 <  1:633112/140‑1 (MQ=255)

                                                                   aCTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTgg                                                                 >  2:1086258/1‑140 (MQ=255)

                                                                     tCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGaa                                                               <  1:249972/140‑1 (MQ=255)

                                                                       gAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACa                                                             <  1:4427/140‑1 (MQ=255)

                                                                        aaaGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAg                                                            >  2:508871/1‑140 (MQ=255)

                                                                          aGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCa                                                          <  2:118345/140‑1 (MQ=255)

                                                                            gcgcATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAat                                                        >  1:1600191/1‑140 (MQ=255)

                                                                               cATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAg                                                            >  1:532550/1‑133 (MQ=255)

                                                                               cATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAg                                                            <  2:532550/133‑1 (MQ=255)

                                                                               cATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATg                                                     >  1:823870/1‑140 (MQ=255)

                                                                               cATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATg                                                     >  1:314428/1‑140 (MQ=255)

                                                                               cATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATg                                                     >  2:518364/1‑140 (MQ=255)

                                                                               cATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATg                                                     >  2:1290353/1‑140 (MQ=255)

                                                                                 tCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTgg                                                                 <  2:413836/126‑1 (MQ=255)

                                                                                 tCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTgg                                                                 >  1:413836/1‑126 (MQ=255)

                                                                                 tCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCa                                                   <  2:823870/140‑1 (MQ=255)

                                                                                 tCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCa                                                   >  1:206149/1‑140 (MQ=255)

                                                                                   cccATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCAcc                                                 <  1:518364/140‑1 (MQ=255)

                                                                                        tttCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGc                                            >  1:687732/1‑140 (MQ=255)

                                                                                         ttCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCa                                           <  1:1086258/140‑1 (MQ=255)

                                                                                         ttCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCa                                           <  2:1126632/140‑1 (MQ=255)

                                                                                         ttCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCa                                           <  2:1180481/140‑1 (MQ=255)

                                                                                          tCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCAt                                          <  1:508871/140‑1 (MQ=255)

                                                                                            gCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGa                                        >  1:1014437/1‑140 (MQ=255)

                                                                                            gCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGa                                        >  1:1040499/1‑140 (MQ=255)

                                                                                                tCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGtt                                    >  2:1611435/1‑140 (MQ=255)

                                                                                                tCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGtt                                    >  1:1931/1‑140 (MQ=255)

                                                                                                 cTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTc                                   <  2:1040499/140‑1 (MQ=255)

                                                                                                    ccGGGGTTTACAAGAACTGGTGGTGGCAAGCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAgg                                <  2:869473/140‑1 (MQ=255)

                                                                                                    ccGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAgg                                >  1:1171623/1‑140 (MQ=255)

                                                                                                    ccGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAgg                                <  2:1931/140‑1 (MQ=255)

                                                                                                    ccGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAgg                                <  1:1524381/140‑1 (MQ=255)

                                                                                                           ttACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAGGTTGAaga                         <  1:1290353/140‑1 (MQ=255)

                                                                                                            tACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAGGTTGAagag                        >  1:19553/1‑140 (MQ=255)

                                                                                                            tACAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAGGTTGAagag                        >  1:1271760/1‑140 (MQ=255)

                                                                                                             aCAAGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAGGTTGAAGAgg                       <  1:632764/140‑1 (MQ=255)

                                                                                                               aaGAACTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAGGTTGAAGAgggg                     <  1:157476/140‑1 (MQ=255)

                                                                                                                  aaCTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAGGTTGAAGAGGGGGGa                  >  1:423468/1‑140 (MQ=255)

                                                                                                                   aCTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATTGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAGGTTGAAGAGGGGGGAt                 >  1:887098/1‑140 (MQ=255)

                                                                                                                   aCTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAGGTTGAAGAGGGGGGAt                 >  2:1463555/1‑140 (MQ=255)

                                                                                                                   aCTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAGGTTGAAGAGGGGGGAt                 >  2:58456/1‑140 (MQ=255)

                                                                                                                   aCTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAGGTTGAAGAGGGGGGAt                 >  1:825277/1‑140 (MQ=255)

                                                                                                                    cTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCa                                  >  1:713115/1‑122 (MQ=255)

                                                                                                                    cTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCa                                  <  2:713115/122‑1 (MQ=255)

                                                                                                                    cTGGTGGTGGCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAGGTTGAAGAGGGGGGAtt                <  2:1014437/140‑1 (MQ=255)

                                                                                                                      ggtggtggCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAGGTTGAAGAGGGGGGATTAc              >  1:731314/1‑140 (MQ=255)

                                                                                                                      ggtggtggCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAGGTTGAAGAGGGGGGATTAc              <  2:894484/140‑1 (MQ=255)

                                                                                                                      ggtggtggCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAGGTTGAAGAGGGGGGATTAc              >  1:1038070/1‑140 (MQ=255)

                                                                                                                      ggtggtggCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAGGTTGAAGAGGGGGGATTAc              <  1:408736/140‑1 (MQ=255)

                                                                                                                       gtggtggCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAGGTTGAAGAGGGGGGATTACt             >  1:742642/1‑140 (MQ=255)

                                                                                                                         ggtggCAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAGGTTGAAGAGGGGGGATTACTGt           <  1:1611435/140‑1 (MQ=255)

                                                                                                                              cAACCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAGGTTGAAGAGGGGGGATTACTGTCTGCg      <  1:517770/140‑1 (MQ=255)

                                                                                                                               aaCCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAGGTTGAAGAGGGGGGATTACTGTCTGCgt     >  1:1026715/1‑140 (MQ=255)

                                                                                                                               aaCCGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAGGTTGAAGAGGGGGGATTACTGTCTGCgt     <  2:1092639/140‑1 (MQ=255)

                                                                                                                                  cGGTGGCTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAGGTTGAAGAGGGGGGATTACTGTCTGCgttgt  <  1:1367286/140‑1 (MQ=255)

AAAACGGCGATGAGCCTGACGCCGATCCCGGGCGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGTTCGTTGCATCGCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAGGTTGAAGAGGGGGGATTACTGTCTGCGTTGT  >  NC_000913/1361361‑1361630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A