Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	139	53.6	1844736	98.1%	1809686	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,538,930	A→G	F1221L (TTC→CTC)	narZ ←	nitrate reductase Z subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,538,930	0	A	G	100.0%	132.6 / NA	38	F1221L (TTC→CTC)	narZ	nitrate reductase Z subunit alpha
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (24/14); total (24/14)

GATACATTTGTCGAGGTTAAGCACCATGCCGACTTGTGAACGGATTTTCATTTTTTCGCCTCCTGTACCTGATCGCGACCTTCATCATCCAGCCAGTTAACGTTCTTCATCTTGCGGATCATGATGAACTCATCGCGGTTCGATCCGACGGTGCCGTAGTAGTTAAAGCCCCAGGCCAGCTGCGCGTAACCGCCAATCATATGCGTTGGTTTCGGGCAAACGCGGGTAACCGAGTTATGAATGCCGCCGCGCATGCC  >  NC_000913/1538803‑1539059

gATACATTTGTCGAGGTTAAGCACCATGCCGACTTGTGAACGGATTTTCATTTTTTCGCCTCCTGTACCTGATCGCGACCTTCATCATCCAGCCAGTTAACGTTCTTCATCTTGCGGATCATGATGAGCTCATCGCGGtt                                                                                                                       <  2:291838/140‑1 (MQ=255)

gATACATTTGTCGAGGTTAAGCACCATGCCGACTTGTGAACGGATTTTCATTTTTTCGCCTCCTGTACCTGATCGCGACCTTCATCATCCAGCCAGTTAACGTTCTTCATCTTGCGGATCATGATGAGCTCATCGCGGtt                                                                                                                       <  2:727784/140‑1 (MQ=255)

gATACATTTGTCGAGGTTAAGCACCATGCCGACTTGTGAACGGATTTTCATTTTTTCGCCTCCTGTACCTGATCGCGACCTTCATCATCCAGCCAGTTAACGTTCTTCATCTTGCGGATCATGATGAGCTCATCGCGGtt                                                                                                                       <  2:453393/140‑1 (MQ=255)

              ggTTAAGCACCATGCCGACTTGTGAACGGATTTTCATTTTTTCGCCTCCTGTACCTGATCGCGACCTTCATCATCCAGCCAGTTAACGTTCTTCATCTTGCGGATCATGATGAGCTCATCGCGGTTCGATCCGACGGTGc                                                                                                         <  2:913163/140‑1 (MQ=255)

               gTTAAGCACCATGCCGACTTGTGAACGGATTTTCATTTTTTCGCCTCCTGTACCTGATCGCGACCTTCATCATCCAGCCAGTTAACGTTCTTCATCTTGCGGATCATGATGAGCTCATCGCGGTTCGATCCGACGGTGcc                                                                                                        >  2:31948/1‑140 (MQ=255)

                    gCACCATGCCGACTTGTGAACGGATTTTCATTTTTTCGCCTCCTGTACCTGATCGCGACCTTCATCATCCAGCCAGTTAACGTTCTTCATCTTGCGGATCATGATGAGCTCATCGCGGTTCGATCCGACGGTGCCgtagt                                                                                                   >  2:286503/1‑140 (MQ=255)

                    gCACCATGCCGACTTGTGAACGGATTTTCA‑TTTTTCGCCTCCTGTACCTGATCGCGACCTTCATCATCCAGCCAGTTAACGTTCTTCATCTTGCGGATCATGATGAGCTCATCGCGGTTCGATCCGACGGTGCCgtagta                                                                                                  >  1:555539/1‑140 (MQ=255)

                        cATGCCGACTTGTGAACGGATTTTCATTTTTTCGCCTCCTGTACCTGATCGCGACCTTCATCATCCAGCCAGTTAACGTTCTTCATATTGCGGATCATGATGAGCTCATCGCGGTTCGATCCGACGGTGCCGTAGTAGtt                                                                                               >  2:526229/1‑140 (MQ=255)

                          tGCCGACTTGTGAACGGATTTTCATTTTTTCGCCTCCTGTACCTGATCGCGACCTTCATCATCCAGCCAGTTAACGTTCTTCATCTTGCGGATCATGATGAGCTCATCGCGGTTCGATCCGACGGTGCCgtag                                                                                                    <  1:396284/133‑1 (MQ=255)

                          tGCCGACTTGTGAACGGATTTTCATTTTTTCGCCTCCTGTACCTGATCGCGACCTTCATCATCCAGCCAGTTAACGTTCTTCATCTTGCGGATCATGATGAGCTCATCGCGGTTCGATCCGACGGTGCCgtag                                                                                                    >  2:396284/1‑133 (MQ=255)

                          tGCCGACTTGTGAACGGATTTTCATTTTTTCGCCTCCTGTACCTGATCGCGACCTTCATCATCCAGCCAGTTAACGTTCTTCATCTTGCGGATCATGATGAGCTCATCGCGGTTCGATCCGACGGTGCCGTAGTAGTTaa                                                                                             >  1:283074/1‑140 (MQ=255)

                          tGCCGACTTGTGAACGGATTTTCATTTTTTCGCCTCCTGTACCTGATCGCGACCTTCATCATCCAGCCAGTTAACGTTCTTCATCTTGCGGATCATGATGAGCTCATCGCGGTTCGATCCGACGGTGCCGTAGTAGTTaa                                                                                             <  1:490405/140‑1 (MQ=255)

                             cGACTTGTGAACGGATTTTCATTTTTTCGCCTCCTGTACCTGATCGCGACCTTCATCATCCAGCCAGTTAACGTTCTTCATCTTGCGGATCATGATGAGCTCATCGCGGTTCGATCCGACGGTGCCGTAGTAGTTAAAGc                                                                                          >  1:54306/1‑140 (MQ=255)

                                      aaCGGATTTTCATTTTTTCGCCTCCTGTACCTGATCGCGACCTTCATCATCCAGCCAGTTAACGTTCTTCATCTTGCGGATCATGATGAGCTCATCGCGGTTCGATCCGACGGTGCCGTAGTAGTTAAAGccc                                                                                        <  2:653202/133‑1 (MQ=255)

                                      aaCGGATTTTCATTTTTTCGCCTCCTGTACCTGATCGCGACCTTCATCATCCAGCCAGTTAACGTTCTTCATCTTGCGGATCATGATGAGCTCATCGCGGTTCGATCCGACGGTGCCGTAGTAGTTAAAGccc                                                                                        >  1:653202/1‑133 (MQ=255)

                                                     tttCGCCTCCTGTACCTGATCGCGCCCTTCATCATCCAGCCAGTTAACGTTCTTCATCTTGCGGATCATGATGAGCTCATCGCGGTTCGATCCGACGGTGCCGTAGTAGTTAAAGCCCCAGGCCAGCTGCGCGTAACCGc                                                                  <  2:147521/140‑1 (MQ=255)

                                                     tttCGCCTCCTGTACCTGATCGCGACCTTCATCATCCAGCCAGTTAACGTTCTTCATCTTGCGGATCATGATGAGCTCATCGCGGTTCGATCCGACGGTGCCGTAGTAGTTAAAGCCCCAGACCAGCTGCGCGTAACCGc                                                                  >  1:355429/1‑140 (MQ=255)

                                                     tttCGCCTCCTGTACCTGATCGCGACCTTCATCATCCAGCCAGTTAACGTTCTTCATCTTGCGGATCATGATGAGCTCATCGCGGTTCGATCCGACGGTGCCGTAGTAGTTAAAGCCCCAGACCAGCTGCGCGTAACCGc                                                                  >  1:644377/1‑140 (MQ=255)

                                                      ttCGCCTCCTGTACCTGATCGCGACCTTCATCACCCAGCCAGTTAACGTTCTTCATCTTGCGGATCATGATGAGCTCATCGCGGTTCGATCCGACGGTGCCGTAGTAGTTAAAGCCCCAGGCCAGCTGCGCGTAACCGcc                                                                 <  1:772660/140‑1 (MQ=255)

                                                                  cccTGATCGCGACCTTCATCATCCAGCCAGTTAACGTTCTTCATCTTGCGGATCATGATGAGCTCATCGCGGTTCGATCCGACGGTGCCGTAGTAGTTAAAGCCCCAGGCCAGCTGCGCGTAACCGCCAATCATATGCGt                                                     <  2:102981/139‑1 (MQ=255)

                                                                       aTCGCGACCTTCATCATCCAGCCAGTTAACGTTCTTCATCTTGCGGATCATGATGAGCTCATCGCGGTTCGATCCGACGGTGCCGTAGTAGTTAAAGCCCCAGGCCAGCTGCGCGTAACCGCCAATCATATGCGTTGGtt                                                >  1:874510/1‑140 (MQ=255)

                                                                        tCGCGACCTTCATCATCCAGCCAGTTAACGTTCTTCATCTTGCGGATCATGATGAGCTCATCGCGGTTCGATCCGACGGTGCCGTAGTAGTTAAAGCCCCAGGCCAGCTGCGCGTAACCGCCAATCATATGCGTTGGttt                                               >  2:205227/1‑140 (MQ=255)

                                                                        tCGCGACCTTCATCATCCAGCCAGTTAACGTTCTTCATCTTGCGGATCATGATGAGCTCATCGCGGTTCGATCCGACGGTGCCGTAGTAGTTAAAGCCCCAGGCCAGCTGCGCGTAACCGCCAATCATATGCGTTGGttt                                               >  1:749238/1‑140 (MQ=255)

                                                                         cgcgACCTTCATCATCCAGCCAGTTAACGTTCTTCATCTTGCGGATCATGATGAGCTCATCGCGGTTCGATCCGACGGTGCCGTAGTAGTTAAAGCCCCAGGCCAGCTGCGCGTAACCGCCAATCATATGCGTTGGTTTc                                              >  1:905838/1‑140 (MQ=255)

                                                                           cgACCTTCATCATCCAGCCAGTTAACGTTCTTCATCTTGCGGATCATGATGAGCTCATCGCGGTTCGATCCGACGGTGCCGTAGTAGTTAAAGCCCCAGGCCAGCTGCGCGTAACCGCCAATCATATGCGTTGGTTTCgg                                            >  1:639180/1‑140 (MQ=255)

                                                                            gACCTTCATCATCCAGCCAGTTAACGTTCTTCATCTTGCGGATCATGATGAGCTCATCGCGGTTCGATCCGACGGTGCCGTAGTAGTTAAAGCCCCAGGCCAGCTGCGCGTAACCGCCAATCATATGCGTTGGTTTCggg                                           >  1:806143/1‑140 (MQ=255)

                                                                              ccTTCATCATCCAGCCAGTTAACGTTCTTCATCTTGCGGATCATGATGAGCTCATCGCGGTTCGATCCGACGGTGCCGTAGTAGTTAAAGCCCCAGGCCAGCTGCGCGTAACCGCCAATCATATGCGTTGGTTTCGGGCa                                         >  1:439968/1‑140 (MQ=255)

                                                                                 tcttgatcCCGCCATTTACCGTTCTTGATCTTGGGGATAATGATGCGCCCATAGGGGTTCGTCCCGAGGGTGCGGTAGTAGTAAAGCCCCCAGCCCAGCTGCGCGTAACCGCCAATCATATGCGTTGGTTTCGGGCAAAc                                      <  2:639180/135‑1 (MQ=255)

                                                                                 tcatcaTCCAGCCAGTTAACGTTCTTCATCTTGCGGATCATGATGAGCTCATCGCGGTTCGATCCGACGGTGCCGTAGTAGTTAAAGCCCCAGGCCAGCTGCGCGTAACCGCCAATCATATGCGTTGGTTTCGGGCAAAc                                      >  2:86196/1‑140 (MQ=255)

                                                                                          agccagTTAACGTTCTTCATCTTGCGGATCATGATGAGCTCATCGCGGTTCGATCCGACGGTGCCGTAGTAGTTAAAGCCCCAGGCCAGCTGCGCGTAACCGCCAATCATATGCGTTGGTTTCGGGCAAACGCGGGTAAc                             <  2:439968/140‑1 (MQ=255)

                                                                                           gccagTTAACGTTCTTCATCTTGCGGATCATGATGAGCTCATCGCGGTTCGATCCGACGGTGCCGTAGTAGTTAAAGCCCCAGGCCAGCTGCGCGTAACCGCCAATCATATGCGTTGGTTTCGGGCAAACGCGGGTAAcc                            <  2:874510/140‑1 (MQ=255)

                                                                                            ccagTTAACGTTCTTCATCTTGCGGATCATGATGAGCTCATCGCGGTTCGATCCGACGGTGCCGTAGTAGTTAAAGCCCCAGGCCAGCTGCGCGTAACCGCCAATCATATGCGTTGGTTTCGGGCAAACGCggg                                 <  2:527325/134‑1 (MQ=255)

                                                                                            ccagTTAACGTTCTTCATCTTGCGGATCATGATGAGCTCATCGCGGTTCGATCCGACGGTGCCGTAGTAGTTAAAGCCCCAGGCCAGCTGCGCGTAACCGCCAATCATATGCGTTGGTTTCGGGCAAACGCggg                                 >  1:527325/1‑134 (MQ=255)

                                                                                            ccagTTAACGTTCTTCATCTTGCGGATCATGATGAGCTCATCGCGGTTCGATCCGACGGTGCCGTAGTAGTTAAAGCCCCAGGCCAGCTGCGCGTAACCGCCAATCATATGCGTTGGTTTCGGGCAAACGCGGGTAACCg                           >  1:622909/1‑140 (MQ=255)

                                                                                            ccagTTAACGTTCTTCATCTTGCGGATCATGATGAGCTCATCGCGGTTCGATCCGACGGTGCCGTAGTAGTTAAAGCCCCAGGCCAGCTGCGCGTAACCGCCAATCATATGCGTTGGTTTCGGGCAAACGCGGGTAACCg                           >  1:52084/1‑140 (MQ=255)

                                                                                                ttAACGTTCTTCATCTTGCGGATCATGATGAGCTCATCGCGGTTCGATCCGACGGTGCCGTAGTAGTTAAAGCCCCAGGCCAGCTGCGCGTAACCGCCAATCATATGCGTTGGTTTCGGGCAAACGCGGGTAACCGAGtt                       <  1:5904/140‑1 (MQ=255)

                                                                                                         ttcATCTTGCGGATCATGATGAGCTCATCGCGGTTCGATCCGACGGTGCCGTAGTAGTTAAAGCCCCAGGCCAGCTGCGCGTAACCGCCAATCATATGCGTTGGTTTCGGGCAAACGCGGGTAACCGAGTTATGAATgcc              <  2:283074/140‑1 (MQ=255)

                                                                                                          tcATCTTGCGGATCATGATGAGCTCATCGCGGTTCGATCCGACGGTGCCGTAGTAGTTAAAGCCCCAGGCCAGCTGCGCGTAACCGCCAATCATATGCGTTGGTTTCGGGCAAACGCGGGTAACCGAGTTATGAATgccg             >  2:450402/1‑140 (MQ=255)

                                                                                                          tcATCTTGCGGATCATGATGAGCTCATCGCGGTTCGATCCGACGGTGCCGTAGTAGTTAAAGCCCCAGGCCAGCTGCGCGTAACCGCCAATCATATGCGTTGGTTTCGGGCAAACGCGGGTAACCGAGTTATGAATgccg             <  2:749238/140‑1 (MQ=255)

                                                                                                                tGCGGATCATGATGAGCTCATCGCGGTTCGATCCGACGGTGCCGTAGTAGTTAAAGCCCCAGGCCAGCTGCGCGTAACCGCCAATCATATGCGTTGGTTTCGGGCAAACGCGGGTAACCGAGTTATGAATGCCGCCgcgc       >  2:473908/1‑140 (MQ=255)

                                                                                                                tGCGGATCATGATGAGCTCATCGCGGTTCGATCCGACGGTGCCGTAGTAGTTAAAGCCCCAGACCAGCTGCGCGTAACCGCCAATCATATGCGTTGGTTTCGGGCAAACGCGGGTAACCGAGTTATGAATGCCGCCgcgc       <  2:355429/140‑1 (MQ=255)

                                                                                                                ggCGGACCATGAGGAGCTGATGGCGGTTGGATCAGCCGGTCCCGTAGTAGTTAAAGCCCAGGACCAGTTGCCGGTAACCGCCAATCATATGCGTTGGTTTCGGGCAAACGCGGGTAACCGAGTTATGAATGCCGCCgcgc       <  2:644377/139‑1 (MQ=255)

                                                                                                                     aTCATGATGAGCTCATCGCGGTTCGATCCGACGGTGCCGTAGTAGTTAAAGCCCCAGGCCAGCTGCGCGTAACCGCCAATCATATGCGTTGGTTTCGGGCAAACGCGGGTAACCGAGTTATGAATGCCGCCGCGCATGcc  >  2:434251/1‑140 (MQ=255)

GATACATTTGTCGAGGTTAAGCACCATGCCGACTTGTGAACGGATTTTCATTTTTTCGCCTCCTGTACCTGATCGCGACCTTCATCATCCAGCCAGTTAACGTTCTTCATCTTGCGGATCATGATGAACTCATCGCGGTTCGATCCGACGGTGCCGTAGTAGTTAAAGCCCCAGGCCAGCTGCGCGTAACCGCCAATCATATGCGTTGGTTTCGGGCAAACGCGGGTAACCGAGTTATGAATGCCGCCGCGCATGCC  >  NC_000913/1538803‑1539059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A