Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	139	53.6	1844736	98.1%	1809686	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,566,171	C→T	G324S (GGT→AGT)	dosC ←	diguanylate cyclase DosC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,566,171	0	C	T	100.0%	219.0 / NA	61	G324S (GGT→AGT)	dosC	diguanylate cyclase DosC
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (34/27); total (34/27)

TCACTACTGCGGACGTTGTCATAAAAGGCCTGAGAGACTTTACGCAGAATTTCATCACCAGTGTTATGGCCCCACGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACCGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGTAATTTCGTCAGTACATCCATACCGACTTCGTGGCGCGATACTTCTTCAAACAATTCACGCAGCAAGGTAATAATT  >  NC_000913/1566038‑1566307

tCACTACTGCGGACGTTGTCATAAAAGGCCTGAGAGACTTTACGCAGAATTTCATCACCAGTGTTATGGCCCCACGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTActggtccg                                                                                                                                    >  1:744053/1‑140 (MQ=255)

    tactGCGGACGTTGTCATAAAAGGCCTGAGAGACTTTACGCAGAATTTCATCACCAGTGTTATGGCCCCACGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTActggtccgg                                                                                                                                   >  1:370981/1‑137 (MQ=255)

    tactGCGGACGTTGTCATAAAAGGCCTGAGAGACTTTACGCAGAATTTCATCACCAGTGTTATGGCCCCACGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTActggtccgg                                                                                                                                   <  2:370981/137‑1 (MQ=255)

      ctGCGGACGTTGTCATAAAAGGCCTGAGAGACTTTACGCAGAATTTCATCACCAGTGTTATGGCCCCACGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGc                                                                                                                              >  2:486041/1‑140 (MQ=255)

      ctGCGGACGTTGTCATAAAAGGCCTGAGAGACTTTACGCAGAATTTCATCACCAGTGTTATGGCCCCACGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGc                                                                                                                              >  2:842235/1‑140 (MQ=255)

         cGGACGTTGTCATAAAAGGCCTGAGAGACTTTACGCAGAATTTCATCACCAGTGTTATGGCCCCACGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTActggtccgg                                                                                                                                   <  2:225756/132‑1 (MQ=255)

         cGGACGTTGTCATAAAAGGCCTGAGAGACTTTACGCAGAATTTCATCACCAGTGTTATGGCCCCACGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTActggtccgg                                                                                                                                   >  1:225756/1‑132 (MQ=255)

         cGGACGTTGTCATAAAAGGCCTGAGAGACTTTACGCAGAATTTCATCACCAGTGTTATGGCCCCACGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATg                                                                                                                           >  2:550221/1‑140 (MQ=255)

           gACGTTGTCATAAAAGGCCTGAGAGACTTTACGCAGAATTTCATCACCAGTGTTATGGCCCCACGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATggg                                                                                                                         >  1:407694/1‑140 (MQ=255)

              gTTGTCATAAAAGGCCTGAGAGACTTTACGCAGAATTTCATCACCAGTGTTATGGCCCCACGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTg                                                                                                                                >  2:728955/1‑130 (MQ=255)

              gTTGTCATAAAAGGCCTGAGAGACTTTACGCAGAATTTCATCACCAGTGTTATGGCCCCACGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTg                                                                                                                                <  1:728955/130‑1 (MQ=255)

              gTTGTCATAAAAGGCCTGAGAGACTTTACGCAGAATTTCATCACCAGTGTTATGGCCCCACGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCaa                                                                                                                      >  1:404010/1‑140 (MQ=255)

              gTTGTCATAAAAGGCCTGAGAGACTTTACGCAGAATTTCATCACCAGTGTTATGGCCCCACGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCaa                                                                                                                      >  1:443198/1‑140 (MQ=255)

              gTTGTCATAAAAGGCCTGAGAGACTTTACGCAGAATTTCATCACCAGTGTTATGGCCCCACGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCaa                                                                                                                      >  2:287539/1‑140 (MQ=255)

                      aaaaGGCCTGAGAGACTTTACGCAGAATTTCATCACCAGTGTTATGGCCCCACGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGt                                                                                                              <  1:550221/140‑1 (MQ=255)

                        aaGGCCTGAGAGACTTTACGCAGAATTTCATCACCAGTGTTATGGCCCCACGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGttt                                                                                                            >  1:132173/1‑140 (MQ=255)

                             cTGAGAGACTTTACGCAGAATTTCATCACCAGTGTTATGGCCCCACGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGa                                                                                                       <  2:404010/140‑1 (MQ=255)

                               gagagaCTTTACGCAGAATTTCATCACCAGTGTTATGGCCCCACGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATa                                                                                                     >  2:51220/1‑140 (MQ=255)

                                      tttACGCAGAATTTCATCACCAGTGTTATGGCCCCACATATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTa                                                                                              >  1:188475/1‑140 (MQ=255)

                                             aGAATTTCATCACCAGTGTTATGGCCCCACGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAAcg                                                                                       <  1:740910/140‑1 (MQ=255)

                                              gAATTTCATCACCAGTGTTATGGCCCCACGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAAcgg                                                                                      <  2:132173/140‑1 (MQ=255)

                                               aaTTTCATCACCAGTGTTATGGCCCCACGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAAcggc                                                                                     >  1:643859/1‑140 (MQ=255)

                                               aaTTTCATCACCAGTGTTATGGCCCCACGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAAcggc                                                                                     >  2:843898/1‑140 (MQ=255)

                                               aaTTTCATCACCAGTGTTATGGCCCCACGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAAcggc                                                                                     >  1:186965/1‑140 (MQ=255)

                                                aTTTCATCACCAGTGTTATGGCCCCACGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAAcggcg                                                                                    <  1:461736/140‑1 (MQ=255)

                                                         ccAGTGTTATGGCCCCACGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGTaa                                                                           >  1:582783/1‑140 (MQ=255)

                                                            gtgtTATGGCCCCACGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGTAAttt                                                                        >  1:88971/1‑140 (MQ=255)

                                                               ttATGGCCCCACGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGTAATTTCGt                                                                     <  1:51220/140‑1 (MQ=255)

                                                               ttATGGCCCCACGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGTAATTTCGt                                                                     <  2:407694/140‑1 (MQ=255)

                                                                  tGGCCCCACGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGTAATTTCGt                                                                     >  2:738733/1‑137 (MQ=255)

                                                                  tGGCCCCACGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGTAATTTCGt                                                                     <  1:738733/137‑1 (MQ=255)

                                                                  tGGCCCCACGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGTAATTTCGTCAg                                                                  <  2:643859/140‑1 (MQ=255)

                                                                    gCCCCACGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGTAATTTCGTCAGTa                                                                <  2:88971/140‑1 (MQ=255)

                                                                       ccACGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGTAATTTCGTCAGTACAt                                                             >  1:281277/1‑140 (MQ=255)

                                                                       ccACGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGTAAATTTTTCAGGACAt                                                             >  1:378686/1‑140 (MQ=255)

                                                                         aCGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTTAGAAATTTTGTCAGTACATcc                                                           >  2:255803/1‑140 (MQ=255)

                                                                         aCGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGTAATTTCGTCAGTACATcc                                                           >  1:508426/1‑140 (MQ=255)

                                                                          cGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTTAGAAAATTTGTCAGTACATccc                                                          >  1:686553/1‑139 (MQ=255)

                                                                          cGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGTAATTTCGTCAGTACATCCa                                                          >  2:610492/1‑140 (MQ=255)

                                                                                  tGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATCTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGTAAATTTGTCAGTACATCCCTACCCAcc                                                  >  2:389844/1‑139 (MQ=255)

                                                                                         tttGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGTAATTTCGTCAGTACATCCATACCGAc                                                   <  1:667607/132‑1 (MQ=255)

                                                                                         tttGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGTAATTTCGTCAGTACATCCATACCGAc                                                   >  2:667607/1‑132 (MQ=255)

                                                                                         tttGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGTAATTTCGTCAGTACATCCATACCGACTTCGTGgc                                           >  1:841017/1‑140 (MQ=255)

                                                                                           tGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGTAATTTCGTCAGTACATCCATACCGACTTCGTGgcgc                                         <  2:744053/140‑1 (MQ=255)

                                                                                                ttATCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGTAATTTCGTCAGTACATCCATACCGACTTCGTGGCGCGATAc                                    <  1:888490/140‑1 (MQ=255)

                                                                                                   tCAACGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGGAAATTCGTCAGTACCTTCATAACGACATTTTGGGGCGATAattt                                 >  1:125148/1‑139 (MQ=255)

                                                                                                       cGTCAATAATCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGTAAATTTGTCCGTACATCCATACCCACTTTGTGGGGCGATActtcttta                             >  2:719203/1‑138 (MQ=255)

                                                                                                           aataatCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGTAATTTCGTCAGTACATCCATACCGACTTCGTGGCGCGATACTTCTTCAAACa                         <  2:508426/140‑1 (MQ=255)

                                                                                                             taatCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGTAATTTTGTCAGTAAATACATACCGACTTCGTGGCGCGGTACTTCTTCAAACaaa                       >  2:406679/1‑139 (MQ=255)

                                                                                                             taatCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGTAATTTCGTCAGTACATCCATACCGACTTCGTGGCGCGATACTTCTTCAAACAAt                       >  1:511224/1‑140 (MQ=255)

                                                                                                               atCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGTAATTTCGTCAGTACATCCATACCGACTTCGTGGCGCGATACTTCTTCAAACAATTc                     <  1:486041/140‑1 (MQ=255)

                                                                                                                tCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGTAATTTCGTCAGTACATCCATACCGACTTCGTGGCGCGATACTTCTTCAAACAATTCa                    <  2:705972/140‑1 (MQ=255)

                                                                                                                tCAGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGTAATTTCGTCAGTACATCCATACCGACTTCGTGGCGCGATACTTCTTCAAACAATTCa                    <  2:841017/140‑1 (MQ=255)

                                                                                                                  aGCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGTAATTTCGTCAGTACATCCATACCGACTTCGTGGCGCGATACTTCTTCAAACAATTCACg                  <  1:828721/140‑1 (MQ=255)

                                                                                                                   gCACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATCTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGTAATTTCGTCAGTACATCCATACCGACTTCGTGGCGCGATACTTCTTCAAACAATTCACgc                 <  1:389844/140‑1 (MQ=255)

                                                                                                                    cACTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGTAATTTTGTCAGTACATCCATACCGACTTCGTGGCGCGGTACTTCTTTAAACAAATCACgca                >  2:828721/1‑140 (MQ=255)

                                                                                                                     aCTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGTAATTTCGTCAGTACATCCATACCGACTTCGTGGCGCGATACTTCTTCAAACAATTCACgcag               <  2:511224/140‑1 (MQ=255)

                                                                                                                     aCTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGTAATTTCGTCAGTACATCCATACCGACTTCGTGGCGCGATACTTCTTCAAACAATTCACgcag               <  2:186965/140‑1 (MQ=255)

                                                                                                                      cTGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGTAATTTCGTCAGTACATCCATACCGACTTCGTGGCGCGATACTTCTTCAAACAATTCACgcagc              <  1:719203/140‑1 (MQ=255)

                                                                                                                      aggACAGTGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGTAATTTCGTCAGTACATCCATACCGACTTCGTGGCGCGATACTTCTTCAAACAATTCACgcagc              <  1:543173/138‑1 (MQ=255)

                                                                                                                       tGACAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGTAATTTCGTCAGTACATCCATACCGACTTCGTGGCGCGATACTTCTTCAAACAATTCACgcagca             <  2:582783/140‑1 (MQ=255)

                                                                                                                         aCAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGTAATTTCGTCAGTACATCCATACCGACTTCGTGGCGCGGTACTTCTTCAAACAAATTACGCAGCAAg           >  2:617333/1‑140 (MQ=255)

                                                                                                                         aCAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGCTTAAGTAATTTCGTCAGTACATCCATACCGACTTCGTGGCGCGATACTTCTTCAAACAATTCACGCAGCAAg           >  1:365915/1‑140 (MQ=255)

                                                                                                                         aCAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGAGGTTAAGTAATTTCGTCAGTACATCCATACCGACTTCGTGGCGCGATACTTCTTCAAACAATTCACgcag               <  1:478258/136‑1 (MQ=255)

                                                                                                                         aCAGCGGTGTACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGAGGTTAAGTAATTTCGTCAGTACATCCATACCGACTTCGTGGCGCGATACTTCTTCAAACAATTCACgcag               >  2:478258/1‑136 (MQ=255)

                                                                                                                                  tACTGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGTAATTTCGTCAGTACATCCATACCGACTTCGTGGCGCGATACTTCTTCAAACAATTCACGCAGCAAGGTAATAAtt  <  1:842235/140‑1 (MQ=255)

TCACTACTGCGGACGTTGTCATAAAAGGCCTGAGAGACTTTACGCAGAATTTCATCACCAGTGTTATGGCCCCACGTATCGTTGATCTCTTTGAATTTATCAACGTCAATAATCAGCACTGACAGCGGTGTACCGGTCCGGTTGGCATGGGCAATTTCGCGTTTGAAGATAGTCGGTAGGAAACGGCGGTTAAGTAATTTCGTCAGTACATCCATACCGACTTCGTGGCGCGATACTTCTTCAAACAATTCACGCAGCAAGGTAATAATT  >  NC_000913/1566038‑1566307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A