Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	148	57.0	1973990	96.6%	1906874	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,041,690	A→G	E358E (GAA→GAG)	appA →	periplasmic phosphoanhydride phosphatase/multiple inositol‑polyphosphate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,041,690	0	A	G	100.0%	177.5 / NA	51	E358E (GAA→GAG)	appA	periplasmic phosphoanhydride phosphatase/multiple inositol‑polyphosphate phosphatase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (25/26); total (25/26)

ACATTACCCACTTCAGTGCTGTTTATCGCCGGACACGATACTAATCTGGCAAATCTCGGCGGCGCACTGGAGCTCAACTGGACGCTTCCCGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAACGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTCGCTGGTCTTCCAGACTTTACAGCAGATGCGTGATAAAACGCCGCTGTCATTAAATACGCCGCCCGGAGAGGTGAAACTGACCCTGGC  >  NC_000913/1041556‑1041821

aCATTACCCACTTCAGTGCTGTTTATCGCCGGACACGATACTAATCTGGCAAATCTCGGCGGCGCACTGGAGCTCAACTGGACGCTTCCCGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCt                                                                                                                                 <  2:719972/139‑1 (MQ=255)

aCATTACCCACTTCAGTGCTGTTTATCGCCGGACACGATACTAATCTGGCAAATCTCGGCGGCGCACTGGAGCTCAACTGGACGCTTCCCGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCt                                                                                                                                 >  1:719972/1‑139 (MQ=255)

aCATTACCCACTTCAGTGCTGTTTATCGCCGGACACGATACTAATCTGGCAAATCTCGGCGGCGCACTGGAGCTCAACTGGACGCTTCCCGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTg                                                                                                                                >  1:26114/1‑140 (MQ=255)

      cccACTTCAGTGCTGTTTATCGCCGGACACGATACT‑ATCTGGCAAATCTCGGCGGCGCACTGGAGCTCAACTGGACGCTTCCCGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTGGCGTCgg                                                                                                                         >  1:137067/1‑140 (MQ=255)

       ccACTTCAGTGCTGTTTATCGCCGGACACGATACTAATCTGGCAAATCTCGGCGGCGCACTGGAGCTCAACTGGACGCTTCCCGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTGGCGTCgg                                                                                                                         <  2:291911/140‑1 (MQ=255)

          cTTCAGTGCTGTTTATCGCCGGACACGATACTAATCTGGCAAATCTCGGCGGCGCACTGGAGCTCAACTGGACGCTTCCCGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTGGCGTCTGCTa                                                                                                                      >  2:57990/1‑140 (MQ=255)

                tGCTGTTTATCGCCGGACACGATACTAATCTGGCAAATCTCGGCGGCGCACTGGAGCTCAACTGGACGCTTCCCGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTGGCg                                                                                                                             >  1:444164/1‑127 (MQ=255)

                tGCTGTTTATCGCCGGACACGATACTAATCTGGCAAATCTCGGCGGCGCACTGGAGCTCAACTGGACGCTTCCCGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTGGCg                                                                                                                             <  2:444164/127‑1 (MQ=255)

                   tGTTTATCGCCGGACACGATACTAATCTGGCAAATCTCGGCGGCGCACTGGAGCTCAACTGGACGCTTCCCGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAAc                                                                                                             >  1:64475/1‑140 (MQ=255)

                     tttATCGCCGGACACGATACTAATCTGGCAAATCTCGGCGGCGCACTGGAGCTCAACTGGACGCTTCCCGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAg                                                                                                           >  2:541767/1‑140 (MQ=255)

                     tttATCGCCGGACACGATACTAATCTGGCAAATCTCGGCGGCGCACTGGAGCTCAACTGGACGCTTCCCGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAg                                                                                                           >  2:712548/1‑140 (MQ=255)

                             cGGACACGATACTAATCTGGCAAATCTCGGCGGCGCACTGGAGCTCAACTGGACGCTTCCCGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGa                                                                                                   >  2:266117/1‑140 (MQ=255)

                               gACACGATACTAATCTGGCAAATCTCGGCGGCGCACTGGAGCTCAACTGGACGCTTCCCGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAg                                                                                                       <  1:202415/134‑1 (MQ=255)

                               gACACGATACTAATCTGGCAAATCTCGGCGGCGCACTGGAGCTCAACTGGACGCTTCCCGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAg                                                                                                       >  2:202415/1‑134 (MQ=255)

                                       aCTAATCTGGCAAATCTCGGCGGCGCACTGGAGCTCAACTGGACGCTTCCCGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTc                                                                                         <  2:620766/140‑1 (MQ=255)

                                       aCTAATCTGGCAAATCTCGGCGGCGCACTGGAGCTCAACTGGACGCTTCCCGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTc                                                                                         >  1:620766/1‑140 (MQ=255)

                                                gCAAATCTCGGCGGCGCACTGGAGCTCAACTGGACGCTTCCCGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTCGCTGGTCtt                                                                                >  2:361247/1‑140 (MQ=255)

                                                gCAAATCTCGGCGGCGCACTGGAGCTCAACTGGACGCTTCCCGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTCGCTGGTCtt                                                                                >  1:142470/1‑140 (MQ=255)

                                                   aaTCTCGGCGGCGCACTGGAGCTCAACTGGACGCTTCCCGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTCGCTGGTCTTCCa                                                                             <  2:26114/140‑1 (MQ=255)

                                                   aaTCTCGGCGGCGCACTGGAGCTCAACTGGACGCTTCCCGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTCGCTGGTCTTCCa                                                                             <  2:142470/140‑1 (MQ=255)

                                                   aaTCTCGGCGGCGCACTGGAGCTCAACTGGACGCTTCCCGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTCGCTGGTCTTCCa                                                                             <  1:361247/140‑1 (MQ=255)

                                                        cggcggCGCACTGGAGCTCAACTGGACGCTTCCCGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTCGCTGGTCTTCCAGACtt                                                                        <  1:236763/140‑1 (MQ=255)

                                                        cggcggCGCACTGGAGCTCAACTGGACGCTTCCCGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTCGCTGGTCTTCCAGACtt                                                                        <  1:541767/140‑1 (MQ=255)

                                                              cgcACTGGAGCTCAACTGGACGCTTCCCGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTCGCTGGTCTTCCAGACTTTAcagc                                                                  >  2:476142/1‑140 (MQ=255)

                                                                cACTGGAGCTCAACTGGACGCTTCCCGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTCGCTGGTCTTCCAGACTTTAcagcag                                                                >  1:3248/1‑140 (MQ=255)

                                                                   tGGAGCTCAACTGGACGCTTCCCGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTCGCTGGTCTTCCAGACTTTACAGCAGATg                                                             <  2:659629/140‑1 (MQ=255)

                                                                     gAGCTCAACTGGACGCTTCCCGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTCGCTGGTCTTCCAGACTTTACAGCAGATGCg                                                           <  2:654503/140‑1 (MQ=255)

                                                                          cAACTGGACGCTTCCCGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTCGCTGGTCTTCCAGACTTTACAGCAGATGCGt                                                          >  1:56575/1‑136 (MQ=255)

                                                                          cAACTGGACGCTTCCCGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTCGCTGGTCTTCCAGACTTTACAGCAGATGCGt                                                          <  2:56575/136‑1 (MQ=255)

                                                                             cTGGACGCTTCCCGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTCGCTGGTCTTCCAGACTTTACAGCAGATGCGTGATaaaa                                                   <  1:4690/140‑1 (MQ=255)

                                                                               ggACGCTTCCCGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTCGCTGGTCTTCCAGACTTTACAGCAGATGCGTGATAAAAcg                                                 >  2:386108/1‑140 (MQ=255)

                                                                                gACGCTTCCCGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTCGCTGGTCTTCCAGACTTTACAGCAGATGCGTGATAAAAcgc                                                >  2:789254/1‑140 (MQ=255)

                                                                                  cGCTTCCCGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTCGCTGGTCTTCCAGACTTTACAGCAGATGCGTGATAAAAcgccg                                              <  2:513324/140‑1 (MQ=255)

                                                                                  cGCTTCCCGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTCGCTGGTCTTCCAGACTTTACAGCAGATGCGTGATAAAAcgccg                                              <  2:3248/140‑1 (MQ=255)

                                                                                     ttCCCGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTCGCTGGTCTTCCAGACTTTACAGCAGATGCGTGATAAAACGCCGCTg                                           <  1:386108/140‑1 (MQ=255)

                                                                                        ccGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTCGCTGGTCTTCCAGACTTTACAGCAGATGCGTGATAAAACGCCGCTGTCa                                        <  1:420230/140‑1 (MQ=255)

                                                                                        ccGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTCGCTGGTCTTCCAGACTTTACAGCAGATGCGTGATAAAACGCCGCTGTCa                                        <  2:64475/140‑1 (MQ=255)

                                                                                          ggTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTCGCTGGTCTTCCAGACTTTACAGCAGATGCGTGATAAAACGCCGCTGTCAtt                                      <  1:599328/140‑1 (MQ=255)

                                                                                                ccGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTCGCTGGTCTTCCAGACTTTACAGCAGATGCGTGATAAAAcgccg                                              >  2:502141/1‑126 (MQ=255)

                                                                                                ccGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTCGCTGGTCTTCCAGACTTTACAGCAGATGCGTGATAAAAcgccg                                              <  1:502141/126‑1 (MQ=255)

                                                                                                          cgccgcCAGGTGGTGAACTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTCGCTGGTCTTCCAGACTTTACAGCAGATGCGTGATAAAACGCCGCTGTCATTAAATACGCCGCCCGga                      >  2:293514/1‑140 (MQ=255)

                                                                                                             cgccAGGTGGTGAACTGGCGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTCGCTGGTCTTCCAGACTTTACAGCAGATGCGTGATAAAACGCCGCTGTCATTAAATACGCCGCCCGGagag                   >  1:708790/1‑140 (MQ=255)

                                                                                                               ccAGGTGGTGAACTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTCGCTGGTCTTCCAGACTTTACAGCAGATGCGTGATAAAACGCCGCTGTCATTAAATACGCCGCCCGGAGAGGt                 <  1:712548/140‑1 (MQ=255)

                                                                                                                      gtgAACTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTCGCTGGTCTTCCAGACTTTACAGCAGATGCGTGATAAAACGCCGCTGTCATTAAATACGCCGCCCGGAGAGGTGAAACTg          >  1:19299/1‑140 (MQ=255)

                                                                                                                         aaCTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTCGCTGGTCTTCCAGACTTTACAGCAGATGCGTGATAAAACGCCGCTGTCATTAAATACGCCGCCCGGAGAGGTGaaa             <  2:190824/134‑1 (MQ=255)

                                                                                                                         aaCTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTCGCTGGTCTTCCAGACTTTACAGCAGATGCGTGATAAAACGCCGCTGTCATTAAATACGCCGCCCGGAGAGGTGaaa             >  1:190824/1‑134 (MQ=255)

                                                                                                                         aaCTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTCGCTGGTCTTCCAGACTTTACAGCAGATGCGTGATAAAACGCCGCTGTCATTAAATACGCCGCCCGGAGAGGTGAAACTGAcc       <  2:137067/140‑1 (MQ=255)

                                                                                                                         aaCTGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTCGCTGGCCTTCCAGACTTTACAGCAGATGCGTGATAAAACGCCGCTGTCATTAAATACGCCGCCCGGAGAGGTGAAACTGAcc       >  2:562872/1‑140 (MQ=255)

                                                                                                                            tGGTGTTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTCGCTGGTCTTCCAGACTTTACAGCAGATGCGTGATAAAACGCCGCTGTCATTAAATACGCCGCCCGGAGAGGTGAAACTGACCCTg    >  1:409006/1‑140 (MQ=255)

                                                                                                                              gtgtTTGAGCGCTGGCGTCTGCTAAGCGATAACAGCCAGTGGATTCAGGTTTCGCTGGTCTTCCAGACTTTACAGCAGATGCGTGATAAAACGCCGCTGTCATTAAATACGCCGCCCGGAGAGGTGAAACTGACCCTGGc  <  1:57990/140‑1 (MQ=255)

                                                                                                                              gtgtTTGAGCGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTCGCTGGTCTTCCAGACTTTACAGCAGATGCGTGATAAAACGCCGCTGTCATTAAATACGCCGCCCGGAGAGGTGAAACTGACCCTGGc  <  2:708790/140‑1 (MQ=255)

ACATTACCCACTTCAGTGCTGTTTATCGCCGGACACGATACTAATCTGGCAAATCTCGGCGGCGCACTGGAGCTCAACTGGACGCTTCCCGGTCAGCCGGATAACACGCCGCCAGGTGGTGAACTGGTGTTTGAACGCTGGCGTCGGCTAAGCGATAACAGCCAGTGGATTCAGGTTTCGCTGGTCTTCCAGACTTTACAGCAGATGCGTGATAAAACGCCGCTGTCATTAAATACGCCGCCCGGAGAGGTGAAACTGACCCTGGC  >  NC_000913/1041556‑1041821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A