Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F28 I1 R1
|
148 |
57.0 |
1973990 |
96.6% |
1906874 |
138.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,192,484 |
A→G |
S50P (TCG→CCG) |
hflD ← |
lysogenization regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,192,484 | 0 | A | G | 100.0%
| 88.8
/ NA
| 26 | S50P (TCG→CCG) | hflD | lysogenization regulator |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (10/16); total (10/16) |
AGCACCATCAAGCTGAGTGTGTAGCGGGTTAATTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGG > NC_000913/1192348‑1192606
|
aGCACCATCAAGCTGAGTGTGTAGCGGGTTAATTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGGGCt > 1:650975/1‑140 (MQ=255)
ccATCAAGCTGAGTGTGTAGCGGGTTAATTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGGGCTgggg < 1:14283/140‑1 (MQ=255)
aaGCTGAGTGTGTAGCGGGTTAATTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGGGCTGGGGTTCat > 2:230182/1‑140 (MQ=255)
gCTGAGTGTGTAGCGGGTTAATTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGGGCTGGGGTTCatat < 1:443246/140‑1 (MQ=255)
tgtgtAGCGGGTTAATTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGGGCTGGGGTTCATATCaataa > 1:298021/1‑140 (MQ=255)
tAGCGGGTTAATTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGGGCTGGGGTTCATATCAATAATACt < 1:230182/140‑1 (MQ=255)
gTTAATTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGGGCTGGGGTTCATATCAATAATACTGTtgag > 1:159804/1‑140 (MQ=255)
gTTAATTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGGGCTGGGGTTCATATCAATAATACTGTtgag > 2:889116/1‑140 (MQ=255)
gTTAATTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGGGCTGGGGTTCATATCAATAATACTGTtgag > 1:287096/1‑140 (MQ=255)
ttAATTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCCCGCGGAGCAGGGTTCCCAGCCCGACGCGCAGTTTGGTTTCGCTACCGCCAAAAACCGCCAGCGTCGGGCTGGGGTTCATATCAATAATACTGTtgagt < 2:476401/140‑1 (MQ=255)
ttAATTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGGGCTGGGGTTCATATCAATAATACTGTtgagt > 1:107489/1‑140 (MQ=255)
aTTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGGGCTGGGGTTCATATCAATAATACTGTtgagtgag < 1:889116/140‑1 (MQ=255)
cGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGGGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACgtgt < 2:298021/140‑1 (MQ=255)
ttaaggCCCTGGCGCCGGCTGCCATTGGCCCCGGCGTGTAGGGTTTCCAGCCGGGCGCGCGGGTTGGGTTCGCTCCCCCCAAAAACCGCCAGCGTCGGGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAg < 2:892599/135‑1 (MQ=255)
aaGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGGGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAgcgc < 2:107489/140‑1 (MQ=255)
ggCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGGGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCa > 1:23606/1‑140 (MQ=255)
ctgctgGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGGGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATcaca > 1:103415/1‑140 (MQ=255)
cGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGGGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCg < 2:287096/140‑1 (MQ=255)
ccGGGCAGGGTTTCCAGCCAGTCGCGCGGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGGGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAg < 2:650975/140‑1 (MQ=255)
cGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGGGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAgt > 2:177928/1‑140 (MQ=255)
aGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGGGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAgttg < 2:39138/140‑1 (MQ=255)
aCGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGGGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGa < 2:775604/140‑1 (MQ=255)
cAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGGGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGAc < 1:49811/140‑1 (MQ=255)
tCGCTACCGCCAAAAACCGCCAGCGTCGGGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGc < 1:577788/140‑1 (MQ=255)
tCGCTACCGCCAAAAACCGCCAGCGTCGGGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGc < 2:325717/140‑1 (MQ=255)
aaaaaCCGCCAGCGTCGGGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAggg < 2:23606/140‑1 (MQ=255)
|
AGCACCATCAAGCTGAGTGTGTAGCGGGTTAATTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGG > NC_000913/1192348‑1192606
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
