Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F28 I1 R1
|
95 |
36.3 |
1231394 |
98.7% |
1215385 |
138.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,010,898 |
C→T |
A312V (GCC→GTC) |
uup → |
ATP‑binding protein with possible role in replication |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,010,898 | 0 | C | T | 100.0%
| 42.6
/ NA
| 14 | A312V (GCC→GTC) | uup | ATP‑binding protein with possible role in replication |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (7/7); total (7/7) |
TGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAA > NC_000913/1010768‑1011004
|
tgGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCATGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTc < 2:441638/140‑1 (MQ=255)
ggATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTcc > 2:514248/1‑140 (MQ=255)
gATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGttt > 1:607916/1‑140 (MQ=255)
tCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTc > 2:307547/1‑140 (MQ=255)
aGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGaaa < 2:214602/140‑1 (MQ=255)
cccGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGtt < 2:587350/140‑1 (MQ=255)
ccGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGttt < 1:341120/140‑1 (MQ=255)
aaTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTa < 2:350211/140‑1 (MQ=255)
aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTAc > 1:141808/1‑140 (MQ=255)
ggCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGt < 1:514248/140‑1 (MQ=255)
gCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGtt > 2:344927/1‑140 (MQ=255)
gTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACgg > 1:332104/1‑140 (MQ=255)
gcgcCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAg < 2:141808/140‑1 (MQ=255)
tGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTGCCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACaaa > 1:312600/1‑140 (MQ=255)
|
TGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAA > NC_000913/1010768‑1011004
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
