Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F28 I1 R1 95 36.3 1231394 98.7% 1215385 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,455,774 A→G N204S (AAC→AGC)  paaC → phenylacetyl‑CoA 1,2‑epoxidase, structural subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,455,7740AG100.0% 97.6 / NA 27N204S (AAC→AGC) paaCphenylacetyl‑CoA 1,2‑epoxidase, structural subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/14);  total (13/14)

TTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAAT  >  NC_000913/1455647‑1455905
                                                                                                                               |                                                                                                                                   
ttGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAGCGAAGCCACAtt                                                                                                                         <  2:15943/140‑1 (MQ=255)
   tgGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAGCGAAGCCACATTGaa                                                                                                                      >  2:336242/1‑140 (MQ=255)
          ttACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAGCGAAGCCACATTGAACGTACCg                                                                                                               >  1:246489/1‑140 (MQ=255)
               gccgAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAGCGAAGCCACATTGAACGTACCGCAaga                                                                                                          <  2:53488/140‑1 (MQ=255)
                   aaCTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGTCGGGATCAGCGAAGCCACATTGAACGTACCGCAAGAGCAg                                                                                                      <  1:189530/140‑1 (MQ=255)
                   aaCTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAGCGAAGCCACATTGAACGTACCGCAAGAGCAg                                                                                                      <  1:283199/140‑1 (MQ=255)
                                    gagaTTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAGCGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCgg                                                                                     <  1:224725/140‑1 (MQ=255)
                                                      aGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAGCGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATAc                                                                   >  2:320036/1‑140 (MQ=255)
                                                      aGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAGCGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATAc                                                                   >  2:532703/1‑140 (MQ=255)
                                                               ggTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAGCGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCt                                                          >  1:525959/1‑140 (MQ=255)
                                                                 tATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAGCGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTg                                                        >  1:279357/1‑140 (MQ=255)
                                                                     gCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAGCGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGAcc                                                    <  2:279357/140‑1 (MQ=255)
                                                                      cGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAGCGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGAccc                                                   <  1:336242/140‑1 (MQ=255)
                                                                                      tttACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAGCGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGc                                   <  1:320036/140‑1 (MQ=255)
                                                                                        tacacgcAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAGCGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAg                                 >  1:382689/5‑140 (MQ=255)
                                                                                        tACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAGCGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAg                                 >  2:478843/1‑140 (MQ=255)
                                                                                            cgcAGCGTGGGAGGCCGAAGTTTTTGCCGGGATCAGCGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTTGACCCATGCTAGCAGAAATGCacgatt                             >  2:579268/1‑135 (MQ=255)
                                                                                            cgcAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAGCGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTAtc                             >  2:308656/1‑140 (MQ=255)
                                                                                                   tGGGAAGCCGAAGTTTTTGCCGGGATCAGCGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCg                      >  1:49936/1‑140 (MQ=255)
                                                                                                           cGAAGTTTTTGCCGGGATCAGCGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGc              >  2:333864/1‑140 (MQ=255)
                                                                                                            gAAGTTTTTGCCGGGATCAGCGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGcc             <  2:525959/140‑1 (MQ=255)
                                                                                                             aaGTTTTTGCCGGGATCAGCGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGccc            <  2:147338/140‑1 (MQ=255)
                                                                                                              aGTTTTTGCCGGGATCAGCGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCTGCGTGTCTTGCCCg           >  2:588046/1‑140 (MQ=255)
                                                                                                                  tttGCCGGGATCAGCGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCa       <  1:532703/140‑1 (MQ=255)
                                                                                                                    tGCCGGGATCAGCGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGc     >  2:562807/1‑140 (MQ=255)
                                                                                                                     gCCGGGATCAGCGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCa    <  2:382689/140‑1 (MQ=255)
                                                                                                                      ccGGGATCAGCGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCaa   <  1:478843/140‑1 (MQ=255)
                                                                                                                       cGGGATCAGCGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAAt  <  2:11869/140‑1 (MQ=255)
                                                                                                                               |                                                                                                                                   
TTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAAT  >  NC_000913/1455647‑1455905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A