Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	157	47.3	1607120	99.4%	1597477	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,332,839	G→A	D598N (GAC→AAC)	topA →	DNA topoisomerase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,332,839	0	G	A	100.0%	115.9 / NA	34	D598N (GAC→AAC)	topA	DNA topoisomerase 1
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/15); total (19/15)

GGCAAATCACGAAGCAGAGTGGAAAGCTGTACTGGATCACTTCTTCTCGGATTTCACCCAGCAGTTAGATAAAGCTGAAAAAGATCCGGAAGAGGGTGGTATGCGCCCGAACCAGATGGTTCTGACCAGCATTGACTGCCCGACTTGTGGTCGCAAAATGGGGATTCGCACAGCGAGCACCGGGGTATTCCTTGGCTGTTCTGGCTATGCGCTGCCGCCGAAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGC  >  NC_000913/1332706‑1332974

ggCAAATCACGAAGCAGAGTGGAAAGCTGTACTGGATCACTTCTTCTCGGATTTCACCCAGCAGTTAGATAAAGCTGCAAAAGATCCGGAAGAGGGTGGTATGCGCCCGAACCAGATGGTTCTGACCAGCATTAACTGcc                                                                                                                                   <  1:794418/140‑1 (MQ=255)

  cAAATCACGAAGCAGAGTGGAAAGCTGTACTGGATCACTTCTTCTCGGATTTCACCCAGCAGTTAGATAAAGCTGAAAAAGATCCGGAAGAGGGTGGTATGCGCCCGAACCAGATGGTTCTGACCAGCATTAACTGCCCg                                                                                                                                 >  2:98535/1‑140 (MQ=255)

                      aaaGCTGTACTGGATCACTTCTTCTCGGATTTCACCCAGCAGTTAGATAAAGCTGAAAAAGATCCGGAAGAGGGTGGTATGCGCCCGAACCAGATGGTTCTGACCAGCATTAACTGCCCGACTTGTGGTCGCAAAATggg                                                                                                             >  2:783741/1‑140 (MQ=255)

                      aaaGCTGTACTGGATCACTTCTTCTCGGATTTCACCCAGCAGTTAGATAAAGCTGAAAAAGATCCGGAAGAGGGTGGTATGCGCCCGAACCAGATGGTTCTGACCAGCATTAACTGCCCGACTTGTGGTCGCAAAATggg                                                                                                             <  1:555169/140‑1 (MQ=255)

                      aaaGCTGTACTGGATCACTTCTTCTCGGATTTCACCCAGCAGCTAGATAAAGCTGAAAAAGATCCGGAAGAGGGTGGTATGCGCCCGAACCAGATGGTTCTGACCAGCATTAACT‑CCCGACTTGTGGTCGCAAAATgggg                                                                                                            >  1:166495/1‑140 (MQ=255)

                                    tCACTTCTTCTCGGATTTCACCCAGCAGTTAGATAAAGCTGAAAAAGATCCGGAAGAGGGTGGTATGCGCCCGAACCAGATGGTTCTGACCAGCATTAACTGCCCGACTTGTGGTCGCAAAATGGGGATTCGCACAGCGa                                                                                               >  1:599594/1‑140 (MQ=255)

                                       cttcttCTCGGATTTCACCCAGCAGTTAGATAAAGCTGAAAAAGATCCGGAAGAGGGTGGTATGCGCCCGAACCAGATGGTTCTGACCAGCATTAACTGCCCGACTTGTGGTCGCAAAATGGGGATTCGCACAGCGAg                                                                                              <  2:136213/138‑1 (MQ=255)

                                       cttcttCTCGGATTTCACCCAGCAGTTAGATAAAGCTGAAAAAGATCCGGAAGAGGGTGGTATGCGCCCGAACCAGATGGTTCTGACCAGCATTAACTGCCCGACTTGTGGTCGCAAAATGGGGATTCGCACAGCGAg                                                                                              >  1:136213/1‑138 (MQ=255)

                                       cttcttCTCGGATTTCACCCAGCAGTTAGATAAAGCTGAAAAAGATCCGGAAGAGGGTGGTATGCGCCCGAACCAGATGGTTCTGACCAGCATTAACTGCCCGACTTGTGGTCGCAAAATGGGGATTCGCACAGCGAGCa                                                                                            >  2:708599/1‑140 (MQ=255)

                                       cttcttCTCGGATTTCACCCAGCAGTTAGATAAAGCTGAAAAAGATCCGGAAGAGGGTGGTATGCGCCCGAACCAGATGGTTCTGACCAGCATTAACTGCCCGACTTGTGGTCGCAAAATGGGGATTCGCACAGCGAGCa                                                                                            >  2:373706/1‑140 (MQ=255)

                                       cttcttCTCGGATTTCACCCAGCAGTTAGATAAAGCTGAAAAAGATCCGGAAGAGGGTGGTATGCGCCCGAACCAGATGGTTCTGACCAGCATTAACTGCCCGACTTGTGGTCGCAAAATGGGGATTCGCACAGCGAGCa                                                                                            <  1:98535/140‑1 (MQ=255)

                                            tctcGGATTTCACCCAGCAGTTAGATAAAGCTGAAAAAGATCCGGAAGAGGGTGGTATGCGCCCGAACCAGATGGTTCTGACCAGCATTAACTGCCCGACTTGTGGTCGCAAAATGGGGATTCGCACAGCGAGCACCggg                                                                                       >  1:122099/1‑140 (MQ=255)

                                              tcGGATTTCACCCAGCAGTTAGATAAAGCTGAAAAAGATCCGGAAGAGGGTGGTATGCGCCCGAACCAGATGGTTCTGACCAGCATTAACTGCCCGACTTGTGGTCGCAAAATGGGGATTCGCACAGCGAGCACCGGGGt                                                                                     <  2:599594/140‑1 (MQ=255)

                                                ggATTTCACCCAGCAGTTAGATAAAGCTGAAAAAGATCCGGAAGAGGGTGGTATGCGCCCGAACCAGATGGTTCTGACCAGCATTAACTGCCCGACTTGTGGTCGCAAAATGGGGATTCGCACAGCGAGCACCgggg                                                                                      <  1:468658/137‑1 (MQ=255)

                                                ggATTTCACCCAGCAGTTAGATAAAGCTGAAAAAGATCCGGAAGAGGGTGGTATGCGCCCGAACCAGATGGTTCTGACCAGCATTAACTGCCCGACTTGTGGTCGCAAAATGGGGATTCGCACAGCGAGCACCgggg                                                                                      >  2:468658/1‑137 (MQ=255)

                                                            gcagTTAGATAAAGCTGAAAAAGATCCGGAAGAGGGTGGTATGCGCCCGAACCAGATGGTTCTGACCAGCATTAACTGCCCGACTTGTGGTCGCAAAATGGGGATTCGCACAGCGAGCACCGGGGTATTCCTTGGCTGtt                                                                       <  1:373706/140‑1 (MQ=255)

                                                                        aGCTGAAAAAGATCCGGAAGAGGGTGGTATGCGCCCGAACCAGATGGTTCTGACCAGCATTAACTGCCCGACTTGTGGTCGCAAAATGGGGATTCGCACAGCGAGCACCGGGGTATTCCTTGGCTGTTCTGGCTATgcgc                                                           >  2:5733/1‑140 (MQ=255)

                                                                        aGCTGAAAAAGATCCGGAAGAGGGAGGTATGCGCCCGAACCAGATGGTTCTGACCAGCATTAACTGCCCGACTTGTGGTCGCAAAATGGGGATTCGCACAGCGAGCACCGGGGTATTCCTTGGCTGTTCTGGCTATgcgc                                                           >  1:84908/1‑140 (MQ=255)

                                                                              aaaaGATCCGGAAGAGGGTGGTATGCGCCCGAACCAGATGGTTCTGACCAGCATTAACTGCCCGACTTGTGGTCGCAAAATGGGGATTCGCACAGCGAGCACCGGGGTATTCCTTGGCTGTTCTGGCTATGCGCTgccgc                                                     >  2:75243/1‑140 (MQ=255)

                                                                                     ccGGAAGAGGGTGGTATGCGCCCGAACCAGATGGTTCTGACCAGCATTAACT‑CCCGACTTGTGGTCGCAAAATGGGGATTCGCACAGCGAGCACCGGGGTATTCCTTGGCTGTTCTGGCTATGCGCTGCCGCCGAAagag                                             <  2:166495/140‑1 (MQ=255)

                                                                                       tccggagGGTGGTATGCGCCCGAACCAGATGGTTCTGACCAGCATTAACTGCCCGACTTGTGGTCGCAAAATGGGGATTCGCACAGCGAGCACCGGGGTATTCCTTGGCTGTTCTGGCTATGCGCTGCCGCCGAAAGAGc                                            <  1:260415/136‑1 (MQ=255)

                                                                                             gggTGGTATGCGCCCGAACCAGATGGTTCTGACCAGCATTAACTGCCCGACTTGTGGTCGCAAAATGGGGATTCGCACAGCGAGCACCGGGGTATTCCTTGGCTGTTCTGGCTATGCGCTGCCGCCGAAAGAGCGTTGCa                                      <  2:84908/140‑1 (MQ=255)

                                                                                              ggtggtATGCGCCCGAACCAGATGGTTCTGACCAGCATTAACTGCCCGACTTGTGGTCGCAAAATGGGGATTCGCACAGCGAGCACCGGGGTATTCCTTGGCTGTTCTGGCTATGCGCTGCCGCCGAAAGAGCGTTGCaa                                     >  2:44470/1‑140 (MQ=255)

                                                                                                 ggtATGCGCCCGAACCAGATGGTTCTGACCAGCATTAACTGCCCGACTTGTGGTCGCAAAATGGGGATTCGCACAGCGAGCACCGGGGTATTCCTTGGCTGTTCTGGCTATGCGCTGCCGCCGAAAGAGCGTTGCAAAac                                  <  2:122099/140‑1 (MQ=255)

                                                                                                            gAACCAGATGGTTCTGACCAGCATTAACTGCCCGACTTGTGGTCGCAAAATGGGGATTCGCACAGCGAGCACCGGGGTATTCCTTGGCTGTTCTGGCTATGCGCTGCCGCCGAAAGAGCGTTGCAAAACCACCATTAAcc                       <  1:75243/140‑1 (MQ=255)

                                                                                                            gAACCAGATGGTTCTGACCAGCATTAACTGCCCGACTTGTGGTCGCAAAATGGGGATTCGCACAGCGAGCACCGGGGTATTCCTTGGCTGTTCTGGCTATGCGCTGCCGCCGAAAGAGCGTTGCAAAACCACCATGAAcc                       >  2:739173/1‑140 (MQ=255)

                                                                                                             aaCCAGATGGTTCTGACCAGCATTAACTGCCCGACTTGTGGTCGCAAAATGGGGATTCGCACAGCGAGCACCGGGGTATTCCTTGGCTGTTCTGGCTATGCGCTGCCGCCGAAAGAGCGTTGCAAAACCACCATTAACCt                      >  2:63599/1‑140 (MQ=255)

                                                                                                                        tCTGACCAGCATTAACTGCCCGACTTGTGGTCGCAAAATGGGGATTCGCACAGCGAGCACCGGGGTATTCCTTGGCTGTTCTGGCTATGCGCTGCCGCCGAAAGAGCGTTGCAAAACCACCATTAAcc                       <  1:9642/128‑1 (MQ=255)

                                                                                                                        tCTGACCAGCATTAACTGCCCGACTTGTGGTCGCAAAATGGGGATTCGCACAGCGAGCACCGGGGTATTCCTTGGCTGTTCTGGCTATGCGCTGCCGCCGAAAGAGCGTTGCAAAACCACCATTAAcc                       >  2:9642/1‑128 (MQ=255)

                                                                                                                        tCTGACCAGCATTAACTGCCCGACTTGTGGTCGCAAAATGGGGATTCGCACAGCGAGCACCGGGGTATTCCTTGGCTGTTCTGGCTATGCGCTGCCGCCGAAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGaaa           >  2:593272/1‑140 (MQ=255)

                                                                                                                           gACCAGCATTAACTGCCCGACTTGTGGTCGCAAAATGGGGATTCGCACAGCGAGCACCGGGGTATTCCTTGGCTGTTCTGGCTATGCGCTGCCGCCGAAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACg        <  1:5733/140‑1 (MQ=255)

                                                                                                                            aCCAGCATTAACTGCCCGACTTGTGGTCGCAAAATGGGGATTCGCACAGCGAGCACCGGGGTATTCCTTGGCTGTTCTGGCTATGCGCTGCCGCCGAAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGa       <  1:708599/140‑1 (MQ=255)

                                                                                                                                 cATTAACTGCCCGACTTGTGGTCGCAAAATGGGGATTCGCACAGCGAGCACCGGGGTATTCCTTGGCTGTTCTGGCTATGCGCTGCCGCCGAAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGc  >  2:441684/1‑140 (MQ=255)

                                                                                                                                 cATTAACTGCCCGACTTGTGGTCGCAAAATGGGGATTCGCACAGCGAGCACCGGGGTATTCCTTGGCTGTTCTGGCTATGCGCTGCCGCCGAAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGc  >  1:418944/1‑140 (MQ=255)

GGCAAATCACGAAGCAGAGTGGAAAGCTGTACTGGATCACTTCTTCTCGGATTTCACCCAGCAGTTAGATAAAGCTGAAAAAGATCCGGAAGAGGGTGGTATGCGCCCGAACCAGATGGTTCTGACCAGCATTGACTGCCCGACTTGTGGTCGCAAAATGGGGATTCGCACAGCGAGCACCGGGGTATTCCTTGGCTGTTCTGGCTATGCGCTGCCGCCGAAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGC  >  NC_000913/1332706‑1332974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A