Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F13 I1 R1 18 2625.4 86379875 99.4% 85861595 141.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,370,378 T→C Y220C (TAC→TGC)  nanE ← putative N‑acetylmannosamine‑6‑phosphate epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,370,3780TC99.4% 10714.9 / 24.5 2551Y220C (TAC→TGC) nanEputative N‑acetylmannosamine‑6‑phosphate epimerase
Reads supporting (aligned to +/- strand):  ref base T (9/7);  new base C (1343/1189);  total (1355/1196)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2623 total aligned reads displayed.

GGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCACCCGACATCCGGCGTCGCTCAACGTTTT  >  NC_000913/3370231‑3370523
                                                                                                                                                   |                                                                                                                                                 
ggCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCAc                                                                                                                                                 <  2:30341409/150‑1 (MQ=255)
ggCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCAc                                                                                                                                                 <  1:30341986/150‑1 (MQ=255)
  cTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCa                                                                                                                                               >  1:30344400/1‑150 (MQ=255)
        gtgtCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGAc                                                                                                                                          >  1:30344460/1‑149 (MQ=255)
         tgtCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACa                                                                                                                                         >  2:30344469/1‑149 (MQ=255)
           tCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAAt                                                                                                                                      <  2:30341272/150‑1 (MQ=255)
            ctggctggctggCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAAt                                                                                                                                      <  2:30342194/149‑1 (MQ=255)
               gctggctggcGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAAtgtg                                                                                                                                   <  1:30341339/149‑1 (MQ=255)
               gctggctggcGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAAtgtg                                                                                                                                   <  1:30341502/149‑1 (MQ=255)
               gctggctggcGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAAtgt                                                                                                                                    <  1:30342279/148‑1 (MQ=255)
               gctggctggcGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAAtg                                                                                                                                     <  2:30341569/147‑1 (MQ=255)
               gctggctggcGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGc                                                                                                                                  >  1:30344506/1‑150 (MQ=255)
                 tggctggcGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCa                                                                                                                                               >  2:30344531/1‑135 (MQ=255)
                   gctggcGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTc                                                                                                                                <  1:30343198/148‑1 (MQ=255)
                   gctggcGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCaa                                                                                                                              <  1:30342454/150‑1 (MQ=255)
                     tggcGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGa                                                                                                                            >  1:30344563/1‑150 (MQ=255)
                       gcGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGa                                                                                                                            <  1:30342725/148‑1 (MQ=255)
                        cGTAGGCAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACaa                                                                                                                                        <  2:30342966/135‑1 (MQ=255)
                         gTAGGAAGTTCACGCCGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGAcgcg                                                                                                                        <  2:30343238/150‑1 (MQ=255)
                         gTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCAc                                                                                                                                                 >  2:30344599/1‑125 (MQ=255)
                           aGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGt                                                                                                                       >  1:30344614/1‑149 (MQ=255)
                            ggAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTg                                                                                                                      <  1:30340765/149‑1 (MQ=255)
                             gAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGa                                                                                                                     <  2:30341896/149‑1 (MQ=255)
                                   cACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAg                                                                                                               >  2:30344660/1‑149 (MQ=255)
                                    acgacgATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGGTTGCAGa                                                                                                              >  1:30344669/1‑149 (MQ=255)
                                    acgacgATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGa                                                                                                              <  2:30342502/149‑1 (MQ=255)
                                    acgacgATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGa                                                                                                              <  2:30342402/149‑1 (MQ=255)
                                      gacgaTCGCGGATCTGCTCGTCAGCGCCCATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACTACTGACAAATGTGCTCAAGACGCGTGATTGCa                                                                                                                <  2:30341806/145‑1 (MQ=255)
                                       acgaTCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAaccg                                                                                                          <  1:30341845/150‑1 (MQ=255)
                                         gaTCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAaccgac                                                                                                        <  1:30342841/150‑1 (MQ=255)
                                         gaTCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAaccga                                                                                                         >  2:30344714/1‑149 (MQ=255)
                                          aTCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAaccgacc                                                                                                       >  1:30344725/1‑150 (MQ=255)
                                          aTCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAaccgacc                                                                                                       <  1:30339025/150‑1 (MQ=255)
                                              cgGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCa                                                                                                   >  1:30344776/1‑150 (MQ=255)
                                              cgGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCa                                                                                                   >  2:30344772/1‑150 (MQ=255)
                                                    tGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGcc                                                                                              >  2:30344825/1‑149 (MQ=255)
                                                     gCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCa                                                                                            >  1:30344842/1‑150 (MQ=255)
                                                       ccGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGccc                                                                                             <  1:30341749/147‑1 (MQ=255)
                                                         gTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGccc                                                                                             >  2:30344883/1‑145 (MQ=255)
                                                          tCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACgcgc                                                                                       >  1:30344888/1‑150 (MQ=255)
                                                            aGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCg                                                                                     <  2:30342097/150‑1 (MQ=255)
                                                              cgcCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCAc                                                                                           <  2:30341036/142‑1 (MQ=255)
                                                                ccAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCACGACGCGt                                                                                                                       >  2:30344937/1‑112 (MQ=255)
                                                                 cAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGgcg                                                                                >  1:30344941/1‑150 (MQ=255)
                                                                       gcgcGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGcgcatcgc                                                                          <  1:30343357/150‑1 (MQ=255)
                                                                       gcgcGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGcgcatcgc                                                                          <  1:30342157/150‑1 (MQ=255)
                                                                        cgcgGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGcgcatcgca                                                                         >  2:30344990/1‑150 (MQ=255)
                                                                        cgcgGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGcgcatcgca                                                                         >  1:30344996/1‑150 (MQ=255)
                                                                            gcggcAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATccg                                                                     <  1:30343065/150‑1 (MQ=255)
                                                                             cggcAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAaccgacc                                                                                                       >  2:30345048/1‑115 (MQ=255)
                                                                             cggcAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATccgc                                                                    >  1:30345044/1‑150 (MQ=255)
                                                                                  aGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCt                                                               <  1:30342545/150‑1 (MQ=255)
                                                                                  aGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCt                                                               >  1:30345093/1‑150 (MQ=255)
                                                                                   gTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCt                                                               >  2:30345111/1‑149 (MQ=255)
                                                                                     ttAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCt                                                               >  1:30345140/1‑147 (MQ=255)
                                                                                       aGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGCCa                                                                                                                                         >  2:30345160/1‑71 (MQ=255)
                                                                                       aGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGc                                                           <  1:30341091/149‑1 (MQ=255)
                                                                                            cgccgATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCgt                                                     >  1:30345207/1‑150 (MQ=255)
                                                                                            cgccgATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAg                                                         >  2:30345211/1‑146 (MQ=255)
                                                                                               cgATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGtgtt                                                  >  1:30345248/1‑150 (MQ=255)
                                                                                                gATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCCTCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAg                                                         >  2:30345268/1‑142 (MQ=255)
                                                                                                gATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGtgttg                                                 <  2:30343089/150‑1 (MQ=255)
                                                                                                gATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGtgttg                                                 <  2:30343695/150‑1 (MQ=255)
                                                                                                    tCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAAc                                             >  1:30345304/1‑150 (MQ=255)
                                                                                                    tCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAAc                                             <  2:30344096/150‑1 (MQ=255)
                                                                                                     cAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATccgc                                                                    >  2:30345320/1‑126 (MQ=255)
                                                                                                        tCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGccc                                         <  1:30342952/150‑1 (MQ=255)
                                                                                                        tCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGccc                                         >  1:30345345/1‑150 (MQ=255)
                                                                                                          gCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCt                                        <  1:30343843/149‑1 (MQ=255)
                                                                                                          gCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCt                                        <  2:30341684/149‑1 (MQ=255)
                                                                                                           ccAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGGgcatcgcatc                                                                       >  2:30345379/1‑117 (MQ=255)
                                                                                                             aGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCgg                                    <  2:30340780/150‑1 (MQ=255)
                                                                                                             aGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCgg                                    >  1:30345399/1‑150 (MQ=255)
                                                                                                               tgtgGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGcgcatcg                                                                           >  2:30345424/1‑109 (MQ=255)
                                                                                                               tgtgGTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCa                                  <  1:30342380/150‑1 (MQ=255)
                                                                                                                   gTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCa                              <  2:30343460/150‑1 (MQ=255)
                                                                                                                   gTCATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCa                              <  1:30341620/150‑1 (MQ=255)
                                                                                                                     cATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCAcc                            >  1:30345469/1‑150 (MQ=255)
                                                                                                                     cATAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGCGTTGTAACGCCCTTCgg                                    >  2:30345470/1‑142 (MQ=255)
                                                                                                                      aTAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCAccc                           <  2:30342797/150‑1 (MQ=255)
                                                                                                                       tAGCACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGGCCGTCACCGCCCACGCGCCGTGgcgc                                                                               >  1:30345510/1‑97 (MQ=255)
                                                                                                                          cACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAGCCGACCGTCACCTCCCACGCGCCGTAGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCa                                  >  2:30345531/1‑139 (MQ=255)
                                                                                                                          cACCGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCACCCGACa                       <  2:30342695/150‑1 (MQ=255)
                                                                                                                             cGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCaa                                 >  2:30345579/1‑137 (MQ=255)
                                                                                                                             cGCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCACCCGACATcc                    >  1:30345570/1‑150 (MQ=255)
                                                                                                                              gCCTTTTTCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAAc                                             <  2:30342581/124‑1 (MQ=255)
                                                                                                                                 tttttCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCACCCGACATCCGGc                 >  1:30345622/1‑149 (MQ=255)
                                                                                                                                  ttttCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCACCCGACATCCGGCGt               >  2:30345630/1‑150 (MQ=255)
                                                                                                                                   tttCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCACCCGACATCCGGCg                <  1:30343567/148‑1 (MQ=255)
                                                                                                                                   tttCATCGCTCTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCACCCGACATCCGGCg                <  1:30342059/148‑1 (MQ=255)
                                                                                                                                    ttCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGc                                                       >  2:30345678/1‑108 (MQ=255)
                                                                                                                                     tCATCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCACCCGACATCCGGCGTCGc            >  1:30345684/1‑150 (MQ=255)
                                                                                                                                        tCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTc                                                                                                    >  1:30345739/1‑59 (MQ=255)
                                                                                                                                        tCGCTGTGTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCACCCGACATCCGGCGTCGCTc          >  2:30345729/1‑149 (MQ=255)
                                                                                                                                            tgtgTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCACCCGACATCCGGCGTCGCTCAACg      >  1:30345789/1‑149 (MQ=255)
                                                                                                                                            tgtgTTGCACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCACCCGACATCCGGCGTCGCTCAACGt     >  2:30345778/1‑150 (MQ=255)
                                                                                                                                              tgttgcACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCACCCGACATCCGGCGTCGCTCAACGtt    <  2:30344407/149‑1 (MQ=255)
                                                                                                                                              tgttgcACCACTGACAAATGCGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCACCCGACATCCGGCGTCGCTCAACGt     <  1:30342626/148‑1 (MQ=255)
                                                                                                                                               gttgcACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATGCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCACCCGACATCCGGCg                <  2:30342312/136‑1 (MQ=255)
                                                                                                                                                ttgcACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCACCCGACATCCGGCGTCGCTCAACGtttt  >  1:30345836/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCACCCGACATCCGGCGTCGCTCAACGTTTT  >  NC_000913/3370231‑3370523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A