Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F13 I1 R1 112 360.8 17302090 99.7% 17250183 98.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,477,888 C→T S14S (AGC→AGT)  dsdX → D‑serine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,477,8880CT100.0% 1535.5 / NA 355S14S (AGC→AGT) dsdXD‑serine transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (195/160);  total (195/160)

Only 100 of 355 total aligned reads displayed.

AGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGG  >  NC_000913/2477790‑2477985
                                                                                                  |                                                                                                 
aGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTAt                                                                                                 >  2:4608852/1‑101 (MQ=255)
aGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTAt                                                                                                 <  2:4608190/101‑1 (MQ=255)
aGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTAt                                                                                                 >  1:4608851/1‑101 (MQ=255)
 gCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATc                                                                                                <  1:4608045/101‑1 (MQ=255)
 gCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATc                                                                                                <  1:4608483/101‑1 (MQ=255)
 gCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATc                                                                                                <  2:4608333/101‑1 (MQ=255)
  ccTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCg                                                                                               <  2:4608684/101‑1 (MQ=255)
   cTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCgt                                                                                              <  2:4608509/101‑1 (MQ=255)
   cTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCgt                                                                                              <  1:4608336/101‑1 (MQ=255)
    tGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCgtg                                                                                             >  2:4608859/1‑101 (MQ=255)
    tGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCgtg                                                                                             <  1:4608238/101‑1 (MQ=255)
     gCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCgtgt                                                                                            >  1:4608861/1‑101 (MQ=255)
       gttgttTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTa                                                                                          >  2:4608866/1‑101 (MQ=255)
       gttgttTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTa                                                                                          <  1:4608306/101‑1 (MQ=255)
        ttgttTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTaa                                                                                         >  1:4608867/1‑101 (MQ=255)
          gttTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAAtt                                                                                       <  1:4608271/101‑1 (MQ=255)
            ttttttGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGt                                                                                     <  2:4608311/101‑1 (MQ=255)
            ttttttGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGt                                                                                     >  2:4608874/1‑101 (MQ=255)
             tttttGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTa                                                                                    >  1:4608876/1‑101 (MQ=255)
              ttttGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTAc                                                                                   >  1:4608879/1‑101 (MQ=255)
              ttttGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTAc                                                                                   >  2:4608881/1‑101 (MQ=255)
                ttGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTg                                                                                 <  2:4608251/101‑1 (MQ=255)
                ttGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTg                                                                                 <  2:4608482/101‑1 (MQ=255)
                  gTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGAc                                                                               >  1:4608888/1‑101 (MQ=255)
                   tCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGAcc                                                                              <  2:4608433/101‑1 (MQ=255)
                    ccAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCa                                                                             <  2:4608275/101‑1 (MQ=255)
                    ccAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCa                                                                             >  2:4608890/1‑101 (MQ=255)
                     cAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCAt                                                                            <  2:4608125/101‑1 (MQ=255)
                     cAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCAt                                                                            >  1:4608894/1‑101 (MQ=255)
                      aaTATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATc                                                                           >  2:4608897/1‑101 (MQ=255)
                        tatCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGt                                                                         >  2:4608904/1‑101 (MQ=255)
                        tatCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGt                                                                         <  1:4608544/101‑1 (MQ=255)
                         atcatcAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTg                                                                        <  2:4607996/101‑1 (MQ=255)
                         atcatcAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTg                                                                        >  1:4608905/1‑101 (MQ=255)
                          tcatcaGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGa                                                                       >  2:4608910/1‑101 (MQ=255)
                              caGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGtt                                                                   <  2:4608296/101‑1 (MQ=255)
                              caGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGtt                                                                   >  2:4608914/1‑101 (MQ=255)
                               aGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTc                                                                  >  1:4608917/1‑101 (MQ=255)
                               aGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTc                                                                  >  2:4608920/1‑101 (MQ=255)
                                ggTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCa                                                                 >  2:4608923/1‑101 (MQ=255)
                                 gTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCaa                                                                <  2:4608442/101‑1 (MQ=255)
                                  ttAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCaaa                                                               <  2:4608240/101‑1 (MQ=255)
                                    aaTCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAAtt                                                             <  2:4608365/101‑1 (MQ=255)
                                    aaTCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAAtt                                                             <  2:4608465/101‑1 (MQ=255)
                                    aaTCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAAtt                                                             <  1:4608387/101‑1 (MQ=255)
                                    aaTCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAAtt                                                             <  1:4608155/101‑1 (MQ=255)
                                      tCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTcc                                                           >  1:4608929/1‑101 (MQ=255)
                                       cacaGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCa                                                          >  2:4608930/1‑101 (MQ=255)
                                        acaGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCAc                                                         >  1:4608934/1‑101 (MQ=255)
                                         caGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCAcc                                                        >  2:4608937/1‑101 (MQ=255)
                                          aGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCAccc                                                       >  2:4608943/1‑101 (MQ=255)
                                            gggAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGt                                                     <  2:4608202/101‑1 (MQ=255)
                                            gggAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGt                                                     <  2:4608141/101‑1 (MQ=255)
                                            gggAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGt                                                     >  1:4608944/1‑101 (MQ=255)
                                             ggAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGtt                                                    <  1:4608421/101‑1 (MQ=255)
                                               aaGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCAcc                                                        >  2:4608950/1‑95 (MQ=255)
                                               aaGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTc                                                  <  2:4608378/101‑1 (MQ=255)
                                                  gTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTgg                                               >  1:4608951/1‑101 (MQ=255)
                                                   tGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGgc                                              <  1:4607803/101‑1 (MQ=255)
                                                    gagaTATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGgcg                                             <  2:4608228/101‑1 (MQ=255)
                                                     agaTATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGgcgc                                            >  1:4608956/1‑101 (MQ=255)
                                                      gaTATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCt                                           <  1:4608288/101‑1 (MQ=255)
                                                      gaTATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCt                                           >  2:4608959/1‑101 (MQ=255)
                                                        tatGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGt                                         >  1:4608962/1‑101 (MQ=255)
                                                          tGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCg                                                                          <  2:4608453/66‑1 (MQ=255)
                                                           gCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGc                                      >  2:4608966/1‑101 (MQ=255)
                                                            cACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCt                                     <  2:4608418/101‑1 (MQ=255)
                                                             aCTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTg                                    >  2:4608971/1‑101 (MQ=255)
                                                              ctctCAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTgg                                   >  1:4608972/1‑101 (MQ=255)
                                                                  cAAATCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAg                               >  2:4608978/1‑101 (MQ=255)
                                                                   aaaTCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGc                              >  1:4608982/1‑101 (MQ=255)
                                                                    aaTCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGct                             >  1:4608985/1‑101 (MQ=255)
                                                                     aTCTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGctt                            <  1:4608367/101‑1 (MQ=255)
                                                                       cTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGcttct                          >  2:4608989/1‑101 (MQ=255)
                                                                       cTGGGTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGcttct                          >  1:4608990/1‑101 (MQ=255)
                                                                         gggTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCttcttc                        >  2:4608995/1‑101 (MQ=255)
                                                                           gTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGt                      >  1:4609000/1‑101 (MQ=255)
                                                                           gTTGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGt                      >  2:4608999/1‑101 (MQ=255)
                                                                            ttGTGAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTg                     <  1:4608346/101‑1 (MQ=255)
                                                                              gtgAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTggg                   >  1:4609008/1‑101 (MQ=255)
                                                                              gtgAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTggg                   >  2:4609006/1‑101 (MQ=255)
                                                                               tgAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGa                  <  2:4608349/101‑1 (MQ=255)
                                                                                gAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGaa                 >  2:4609011/1‑101 (MQ=255)
                                                                                gAGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGaa                 <  2:4608589/101‑1 (MQ=255)
                                                                                 aGCACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAAc                <  1:4608499/101‑1 (MQ=255)
                                                                                   cACGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACga              >  1:4609016/1‑101 (MQ=255)
                                                                                    aCGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACgat             >  1:4609019/1‑101 (MQ=255)
                                                                                     cGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACgatg            >  2:4609025/1‑101 (MQ=255)
                                                                                     cGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACgatg            >  1:4609027/1‑101 (MQ=255)
                                                                                     cGCTGCTTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACgatg            <  2:4608406/101‑1 (MQ=255)
                                                                                      gctgctTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACgatga           >  1:4609030/1‑101 (MQ=255)
                                                                                       ctgctTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACgatgat          >  2:4609032/1‑101 (MQ=255)
                                                                                        tgctTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGatgatg         >  2:4609037/1‑101 (MQ=255)
                                                                                          ctTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATggg       <  1:4608439/101‑1 (MQ=255)
                                                                                          ctTATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATggg       <  1:4608773/101‑1 (MQ=255)
                                                                                           ttATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGc      <  1:4608602/101‑1 (MQ=255)
                                                                                            tATCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCa     >  1:4609041/1‑101 (MQ=255)
                                                                                             aTCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCAt    <  2:4608516/101‑1 (MQ=255)
                                                                                              tCAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATg   >  1:4609046/1‑101 (MQ=255)
                                                                                               cAGTATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATgg  <  1:4608324/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
AGCCTGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGG  >  NC_000913/2477790‑2477985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A