Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F13 I1 R1 112 360.8 17302090 99.7% 17250183 98.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,737,496 T→C intergenic (+1/‑103) raiA → / → pheL stationary phase translation inhibitor and ribosome stability factor/phe operon leader peptide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,737,4960TC99.7% 1311.2 / NA 311intergenic (+1/‑103)raiA/pheLstationary phase translation inhibitor and ribosome stability factor/phe operon leader peptide
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (138/172);  total (139/172)
Fisher's exact test for biased strand distribution p-value = 4.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 328 total aligned reads displayed.

AACGGCAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGTCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGGTACTGTACTAAAGTCACTTAAGGAA  >  NC_000913/2737398‑2737592
                                                                                                  |                                                                                                
aaCGGCAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGccc                                                                                                <  1:5073951/101‑1 (MQ=255)
aaCGGCAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGccc                                                                                                <  1:5073739/101‑1 (MQ=255)
 aCGGCAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCt                                                                                               >  1:5074201/1‑101 (MQ=255)
  cGGCAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCtt                                                                                              <  1:5073280/101‑1 (MQ=255)
     cAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTtat                                                                                           >  2:5074205/1‑101 (MQ=255)
     cAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTtat                                                                                           <  1:5073563/101‑1 (MQ=255)
      aGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTtata                                                                                          <  2:5073268/101‑1 (MQ=255)
      aGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTtata                                                                                          <  2:5073406/101‑1 (MQ=255)
         tCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTg                                                                                       >  1:5074213/1‑101 (MQ=255)
          cAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGa                                                                                      <  2:5073628/101‑1 (MQ=255)
           aaTAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAg                                                                                     >  1:5074216/1‑101 (MQ=255)
            aTAAATTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGccc                                                                                                >  1:5074219/1‑89 (MQ=255)
            aTAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAgt                                                                                    <  1:5073789/101‑1 (MQ=255)
             tAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAgtg                                                                                   >  2:5074221/1‑101 (MQ=255)
               aaCTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTa                                                                                 <  2:5073709/101‑1 (MQ=255)
                aCTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTAt                                                                                >  2:5074224/1‑101 (MQ=255)
                   gcagcaCAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGc                                                                             >  1:5074228/1‑101 (MQ=255)
                     agcaCAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCa                                                                           >  1:5074234/1‑101 (MQ=255)
                       cacaAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAAc                                                                         <  1:5073708/101‑1 (MQ=255)
                       cacaAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAAc                                                                         >  2:5074236/1‑101 (MQ=255)
                         caAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAAcgc                                                                       <  1:5073455/101‑1 (MQ=255)
                          aaaGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCaa                                                                          >  1:5074241/1‑97 (MQ=255)
                          aaaGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAAcgcg                                                                      <  2:5073583/101‑1 (MQ=255)
                           aaGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACgcgc                                                                     >  2:5074242/1‑101 (MQ=255)
                           aaGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACgcgc                                                                     <  2:5073648/101‑1 (MQ=255)
                            aGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGcc                                                                    <  2:5073518/101‑1 (MQ=255)
                               cGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTc                                                                 <  2:5073891/101‑1 (MQ=255)
                               cGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTc                                                                 <  1:5073652/101‑1 (MQ=255)
                                gAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCg                                                                >  1:5074246/1‑101 (MQ=255)
                                 aaGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAAcg                                                                        <  1:5073676/92‑1 (MQ=255)
                                 aaGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCgg                                                               <  2:5073665/101‑1 (MQ=255)
                                 aaGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCgg                                                               >  2:5074250/1‑101 (MQ=255)
                                    cACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGgcg                                                            <  1:5073472/101‑1 (MQ=255)
                                     aCGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTc                                                                 >  1:5074257/1‑95 (MQ=255)
                                     aCGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGgcgc                                                           >  2:5074254/1‑101 (MQ=255)
                                      cgtcgtGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTatat                                                                                         <  1:5074075/70‑1 (MQ=255)
                                      cgtcgtGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCg                                                                >  2:5074260/1‑95 (MQ=255)
                                       gtcgtGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGt                                                         >  1:5074264/1‑101 (MQ=255)
                                       gtcgtGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGt                                                         <  2:5073465/101‑1 (MQ=255)
                                         cgtGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCaa                                                                          >  1:5074268/1‑82 (MQ=255)
                                         cgtGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGttt                                                       <  2:5073535/101‑1 (MQ=255)
                                         cgtGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGttt                                                       >  2:5074266/1‑101 (MQ=255)
                                          gtGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGtttt                                                      <  2:5073794/101‑1 (MQ=255)
                                           tGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGttttt                                                     >  2:5074274/1‑101 (MQ=255)
                                           tGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCCGGCGCGgtttt                                                     >  1:5074275/1‑101 (MQ=255)
                                           tGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGATGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCgg                                                               <  1:5074008/91‑1 (MQ=255)
                                            gCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGtttttt                                                    <  1:5073759/101‑1 (MQ=255)
                                            gCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGtttttt                                                    <  1:5073516/101‑1 (MQ=255)
                                             ccGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGgttttt                                                    >  1:5074279/1‑100 (MQ=255)
                                             ccGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTttg                                                   <  2:5073418/101‑1 (MQ=255)
                                               gCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTttgtt                                                 >  2:5074283/1‑101 (MQ=255)
                                                   cATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCaa                                                                          >  1:5074288/1‑72 (MQ=255)
                                                   cATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCa                                                                           >  2:5074289/1‑71 (MQ=255)
                                                   cATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACa                                             <  2:5073690/101‑1 (MQ=255)
                                                   cATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACa                                             <  2:5073595/101‑1 (MQ=255)
                                                      cGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGgc                                                             >  2:5074294/1‑82 (MQ=255)
                                                      cGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCg                                          <  1:5073850/101‑1 (MQ=255)
                                                       ggTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTc                                                                 <  1:5073610/77‑1 (MQ=255)
                                                       ggTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGt                                         <  2:5073679/101‑1 (MQ=255)
                                                        gTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTg                                        >  1:5074296/1‑101 (MQ=255)
                                                        gTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTg                                        <  1:5073616/101‑1 (MQ=255)
                                                            aaGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGaaaa                                    <  2:5073613/101‑1 (MQ=255)
                                                             aGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAAc                                   <  2:5073761/101‑1 (MQ=255)
                                                             aGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAAc                                   <  1:5073662/101‑1 (MQ=255)
                                                              gACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGgcg                                                            >  1:5074301/1‑75 (MQ=255)
                                                                 gCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTa                               >  2:5074304/1‑101 (MQ=255)
                                                                  ccAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTAc                              <  1:5073773/101‑1 (MQ=255)
                                                                  ccAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTAc                              >  1:5074306/1‑101 (MQ=255)
                                                                  ccAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTAc                              <  2:5073723/101‑1 (MQ=255)
                                                                  ccAACTTCGTCGAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTAc                              <  2:5073346/101‑1 (MQ=255)
                                                                        tcgtcgAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGgtac                        <  1:5073594/101‑1 (MQ=255)
                                                                         cgtcgAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGGGTGAAAACAGTAc                              >  1:5074310/1‑94 (MQ=255)
                                                                         cgtcgAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGgtact                       <  2:5073907/101‑1 (MQ=255)
                                                                         cgtcgAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGgtact                       <  2:5074025/101‑1 (MQ=255)
                                                                         cgtcgAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGgtact                       <  2:5074130/101‑1 (MQ=255)
                                                                          gtcgAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACg                             <  1:5073722/94‑1 (MQ=255)
                                                                           tcgAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGAc                                              >  2:5074313/1‑76 (MQ=255)
                                                                           tcgAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGgtactgt                     <  2:5073823/101‑1 (MQ=255)
                                                                            cgAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAAcgc                                                                       >  1:5074319/1‑50 (MQ=255)
                                                                            cgAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGgtactgta                    >  2:5074317/1‑101 (MQ=255)
                                                                            cgAAGAAGTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGgtactgta                    <  1:5073752/101‑1 (MQ=255)
                                                                               agaagTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGGTACTGTACTa                 >  2:5074326/1‑101 (MQ=255)
                                                                               agaagTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGGTACTGTACTa                 <  2:5073499/101‑1 (MQ=255)
                                                                               agaagTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGGTACTGTACTa                 >  1:5074324/1‑101 (MQ=255)
                                                                                gaagTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGGTACTGTACTaa                <  2:5073636/101‑1 (MQ=255)
                                                                                 aagTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGgcgc                                                           >  2:5074330/1‑57 (MQ=255)
                                                                                 aagTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGGTACTGTACTaaa               <  2:5073730/101‑1 (MQ=255)
                                                                                  agTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGGTACTGTACTAAAg              <  1:5073833/101‑1 (MQ=255)
                                                                                  agTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGGTACTGTACTAAAg              <  2:5073803/101‑1 (MQ=255)
                                                                                   gTTGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTa                               >  1:5074332/1‑83 (MQ=255)
                                                                                    ttGAAGAAGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGGTACTGTACTAAAGTc            <  1:5073635/101‑1 (MQ=255)
                                                                                      gaagaaGAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGGTACTGTACTAAAGTCAc          <  1:5073571/101‑1 (MQ=255)
                                                                                       aagaagAGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGGTACTGTACTAAAGTCACt         >  2:5074337/1‑101 (MQ=255)
                                                                                        agaagaGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGGTACTGTACTAAAGTCACtt        >  1:5074339/1‑101 (MQ=255)
                                                                                        agaagaGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGGTACTGTACTAAAGTCACtt        <  1:5073439/101‑1 (MQ=255)
                                                                                         gaagaGTAGCCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGgcg                                                            >  2:5074341/1‑48 (MQ=255)
                                                                                             agtagcCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGGTACTGTACTAAAGTCACTTaa      >  2:5074349/1‑98 (MQ=255)
                                                                                             agtagcCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGGTACTGTACTAAAGTCACTTAAGGa   >  1:5074347/1‑101 (MQ=255)
                                                                                             agtagcCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGGTACTGTACTAAAGTCACTTAAGGa   <  1:5073493/101‑1 (MQ=255)
                                                                                              gtagcCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGGTACTGTACTAAAGTCACTTAAGGaa  <  1:5073862/101‑1 (MQ=255)
                                                                                                  |                                                                                                
AACGGCAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGTCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGGTACTGTACTAAAGTCACTTAAGGAA  >  NC_000913/2737398‑2737592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A