Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F13 I1 R1	116	362.0	17214306	99.7%	17162663	98.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,498,917	A→G	I83V (ATT→GTT)	ypdA →	sensory histidine kinase YpdA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,498,917	0	A	G	88.7%	988.0 / 93.4	309	I83V (ATT→GTT)	ypdA	sensory histidine kinase YpdA
Reads supporting (aligned to +/- strand): ref base A (13/22); new base G (111/163); total (124/185)
Fisher's exact test for biased strand distribution p-value = 8.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 315 total aligned reads displayed.

CCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATCGGCGGCGTGACGGCGATCCCCTGCTTTAT  >  NC_000913/2498819‑2499017

ccATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGtt                                                                                                    <  2:4503611/101‑1 (MQ=255)

 cATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTc                                                                                                   <  2:4503324/101‑1 (MQ=255)

 cATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTc                                                                                                   >  2:4504102/1‑101 (MQ=255)

  aTTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCt                                                                                                  <  1:4503650/101‑1 (MQ=255)

  aTTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCt                                                                                                  >  1:4504105/1‑101 (MQ=255)

     ttCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGtt                                                                                               <  2:4503466/101‑1 (MQ=255)

      tctcGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGttt                                                                                              >  1:4504112/1‑101 (MQ=255)

       ctcGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTg                                                                                             >  2:4504113/1‑101 (MQ=255)

       ctcGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTg                                                                                             <  1:4503364/101‑1 (MQ=255)

        tcGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTgg                                                                                            <  2:4503640/101‑1 (MQ=255)

          gCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGcc                                                                                          >  2:4504118/1‑101 (MQ=255)

          gCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGcc                                                                                          <  2:4503778/101‑1 (MQ=255)

           cTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGccc                                                                                         <  2:4503655/101‑1 (MQ=255)

           cTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGccc                                                                                         >  1:4504120/1‑101 (MQ=255)

            tGTTCGCCCTGTTCAGCACCTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCg                                                                                        <  1:4503537/101‑1 (MQ=255)

               tCGCCCTGTTCAGCACCTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTgg                                                                                     >  1:4504126/1‑101 (MQ=255)

               tCGCCCTGTTCAGCACCTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTgg                                                                                     <  1:4503703/101‑1 (MQ=255)

                 gCCCTGTTCAGCACCTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGt                                                                                   >  1:4504129/1‑101 (MQ=255)

                  cccTGTTCAGCACCTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTa                                                                                  <  2:4503440/101‑1 (MQ=255)

                  cccTGTTCAGCACCTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTa                                                                                  >  1:4504132/1‑101 (MQ=255)

                   ccTGTTCAGCACCTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAg                                                                                 >  2:4504134/1‑101 (MQ=255)

                   ccTGTTCAGCACCTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAg                                                                                 >  1:4504138/1‑101 (MQ=255)

                     tGTTCAGCACCTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGc                                                                               <  1:4503662/101‑1 (MQ=255)

                       ttCAGCACCTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGcat                                                                             <  2:4503715/101‑1 (MQ=255)

                        tCAGCACCTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGcatc                                                                            >  2:4504142/1‑101 (MQ=255)

                        tCAGCACCTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGcatc                                                                            >  1:4504141/1‑101 (MQ=255)

                         cAGCACCTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGcatca                                                                           <  1:4503411/101‑1 (MQ=255)

                           gCACCTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCAtt                                                                         <  2:4503496/101‑1 (MQ=255)

                            cACCTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTa                                                                        <  1:4503560/101‑1 (MQ=255)

                            cACCTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGAGGGTAGGCATCATTa                                                                        <  2:4504147/101‑1 (MQ=255)

                             aCCTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGc                                                                                           <  1:4503578/81‑1 (MQ=255)

                              ccTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTAcc                                                                      <  2:4503572/101‑1 (MQ=255)

                              ccTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTAcc                                                                      >  1:4504153/1‑101 (MQ=255)

                              ccTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTAcc                                                                      >  2:4504152/1‑101 (MQ=255)

                              ccTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTAcc                                                                      <  1:4503742/101‑1 (MQ=255)

                                tGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCgg                                                                    <  1:4503479/101‑1 (MQ=255)

                                 ggTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGc                                                                   <  1:4503209/101‑1 (MQ=255)

                                     cGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTAcc                                                                      >  1:4504161/1‑94 (MQ=255)

                                     cGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGa                                                               >  2:4504159/1‑101 (MQ=255)

                                     cGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGa                                                               <  2:4503554/101‑1 (MQ=255)

                                     cGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGa                                                               <  2:4503678/101‑1 (MQ=255)

                                       gCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTg                                                                                             <  1:4503503/69‑1 (MQ=255)

                                       gCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGAtt                                                             >  1:4504165/1‑101 (MQ=255)

                                       gCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGAtt                                                             <  1:4503522/101‑1 (MQ=255)

                                        cGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTg                                                            <  1:4503888/101‑1 (MQ=255)

                                         gTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTg                                                            >  2:4504167/1‑100 (MQ=255)

                                         gTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGc                                                           <  2:4503223/101‑1 (MQ=255)

                                         gTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGc                                                           <  1:4503623/101‑1 (MQ=255)

                                           tCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCgg                                                         >  1:4504171/1‑101 (MQ=255)

                                             ccGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGt                                                       <  2:4503784/101‑1 (MQ=255)

                                              cGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAgg                                                                                <  1:4503671/75‑1 (MQ=255)

                                               gTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTa                                                      <  1:4503912/100‑1 (MQ=255)

                                                tAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAgg                                                                                >  2:4504176/1‑73 (MQ=255)

                                                tAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTAtt                                                    >  1:4504175/1‑101 (MQ=255)

                                                tAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTAtt                                                    <  1:4504096/101‑1 (MQ=255)

                                                tAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTAtt                                                    <  2:4503840/101‑1 (MQ=255)

                                                 aGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTc                                                   <  1:4503344/101‑1 (MQ=255)

                                                 aGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTc                                                   >  2:4504179/1‑101 (MQ=255)

                                                 aGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTc                                                   <  1:4503634/101‑1 (MQ=255)

                                                   aaGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCt                                                                                                  <  2:4503404/52‑1 (MQ=255)

                                                    aGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCAcc                                                >  2:4504183/1‑101 (MQ=255)

                                                       cTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCgg                                              >  2:4504186/1‑100 (MQ=255)

                                                       cTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGt                                             <  2:4503603/101‑1 (MQ=255)

                                                        tCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTa                                            <  2:4503621/101‑1 (MQ=255)

                                                         cGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTAt                                           <  1:4503721/101‑1 (MQ=255)

                                                          gCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTAtt                                          >  1:4504189/1‑101 (MQ=255)

                                                           cTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTAttt                                         <  2:4503725/101‑1 (MQ=255)

                                                            tGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTa                                        >  2:4504192/1‑101 (MQ=255)

                                                              gTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAAt                                      >  2:4504197/1‑101 (MQ=255)

                                                              gTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAAt                                      <  1:4503383/101‑1 (MQ=255)

                                                              gTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAAt                                      <  1:4503614/101‑1 (MQ=255)

                                                               tGAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAAtt                                     <  1:4503809/101‑1 (MQ=255)

                                                                gAACGTACGTATTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTg                                    <  1:4503692/101‑1 (MQ=255)

                                                                   cgtacgtaTTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGata                                 <  2:4503648/101‑1 (MQ=255)

                                                                   cgtacgtaTTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCggg                                                        >  1:4504200/1‑78 (MQ=255)

                                                                     tacgtaTTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATc                               >  2:4504203/1‑101 (MQ=255)

                                                                     tacgtaTTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATc                               >  1:4504206/1‑101 (MQ=255)

                                                                      acgtaTTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATcg                              >  2:4504209/1‑101 (MQ=255)

                                                                      acgtaTTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATcg                              >  1:4504211/1‑101 (MQ=255)

                                                                        gtaTTATCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATcggc                            <  1:4503592/101‑1 (MQ=255)

                                                                         tattatCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATcggcg                           <  2:4503525/101‑1 (MQ=255)

                                                                         tattatCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATcggcg                           >  1:4504214/1‑101 (MQ=255)

                                                                           ttatCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATCggcggc                         <  2:4503685/101‑1 (MQ=255)

                                                                           ttatCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATCggcggc                         <  2:4503760/101‑1 (MQ=255)

                                                                           ttatCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATCggcggc                         <  1:4503787/101‑1 (MQ=255)

                                                                            tatCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATCGgcggcg                        <  2:4503851/101‑1 (MQ=255)

                                                                              tCGCGGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATCGGCGGCGTg                      <  1:4503772/101‑1 (MQ=255)

                                                                               cgcgGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATCGGCGGCGTGa                     <  2:4503818/101‑1 (MQ=255)

                                                                                gcgGTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATCGGCGGCGTGAc                    <  2:4503747/101‑1 (MQ=255)

                                                                                  ggTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATCGGCGGCGTGACgg                  >  2:4504220/1‑101 (MQ=255)

                                                                                  ggTGATGTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATCGGCGGCGTGACgg                  >  1:4504219/1‑101 (MQ=255)

                                                                                   gTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATCGGCGGCGTGACGGc                 <  1:4503441/101‑1 (MQ=255)

                                                                                        gTCCGGCGGGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATCGGCGGCGTGACgg                  >  2:4504224/1‑95 (MQ=255)

                                                                                           cggcggGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATCGGCGGCGTGACGGCGATCCCCt         <  1:4503756/101‑1 (MQ=255)

                                                                                           cggcggGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATCGGCGGCGTGACGGCGATCCCCt         <  1:4503428/101‑1 (MQ=255)

                                                                                           cggcggGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATCGGCGGCGTGACGGCGATCCACt         <  2:4503951/101‑1 (MQ=255)

                                                                                            ggcggGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATCGGCGGCGTg                      >  2:4504227/1‑87 (MQ=255)

                                                                                            ggcggGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATCGGCGGCGTGACGGCGATCCCCTg        <  2:4503734/101‑1 (MQ=255)

                                                                                            ggcggGGTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATCGGCGGCGTGACGGCGATCCCCTg        <  1:4503833/101‑1 (MQ=255)

                                                                                               ggggTTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATCGGCGGCGTGACGGCGATCCCCTGCtt     >  1:4504232/1‑101 (MQ=255)

CCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATCGGCGGCGTGACGGCGATCCCCTGCTTTAT  >  NC_000913/2498819‑2499017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A