Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F13 I1 R1 116 362.0 17214306 99.7% 17162663 98.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,701,448 T→C V94V (GTA→GTG)  pdxJ ← pyridoxine 5'‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,701,4480TC99.6% 1052.3 / ‑4.6 257V94V (GTA→GTG) pdxJpyridoxine 5'‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (166/90);  total (166/91)
Fisher's exact test for biased strand distribution p-value = 3.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 257 total aligned reads displayed.

CCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGAC  >  NC_000913/2701350‑2701545
                                                                                                  |                                                                                                 
ccGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGTCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCAc                                                                                                 >  2:4866854/1‑101 (MQ=255)
 cGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCAcc                                                                                                >  2:4866857/1‑101 (MQ=255)
   gCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAg                                                                                              <  2:4866627/101‑1 (MQ=255)
    cATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAgg                                                                                             <  2:4866425/101‑1 (MQ=255)
    cATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAgg                                                                                             >  1:4866862/1‑101 (MQ=255)
    cATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAgg                                                                                             <  1:4866458/101‑1 (MQ=255)
     aTCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGgc                                                                                            >  1:4866864/1‑101 (MQ=255)
     aTCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGgc                                                                                            >  2:4866865/1‑101 (MQ=255)
       cTGCCAGACGTTTGCAGGCATCGGGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGgcag                                                                                          >  2:4866873/1‑101 (MQ=255)
       cTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGgcag                                                                                          >  1:4866876/1‑101 (MQ=255)
       cTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGgcag                                                                                          >  1:4866872/1‑101 (MQ=255)
       cTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGgcag                                                                                          <  1:4866590/101‑1 (MQ=255)
          ccAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCaa                                                                                       >  1:4866883/1‑101 (MQ=255)
          ccAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCaa                                                                                       >  1:4866881/1‑101 (MQ=255)
           cAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCaaa                                                                                      <  1:4866539/101‑1 (MQ=255)
           cAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCaaa                                                                                      <  1:4866297/101‑1 (MQ=255)
             gACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCaaaaa                                                                                    >  1:4866886/1‑101 (MQ=255)
                 tttGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAAtgtg                                                                                <  1:4866653/101‑1 (MQ=255)
                  ttGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTgg                                                                               >  2:4866888/1‑101 (MQ=255)
                  ttGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTgg                                                                               <  1:4866448/101‑1 (MQ=255)
                   tGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGc                                                                              >  2:4866890/1‑101 (MQ=255)
                     cAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCtt                                                                            <  1:4866375/101‑1 (MQ=255)
                      aGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTc                                                                           >  1:4866894/1‑101 (MQ=255)
                       ggCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCg                                                                          <  1:4866690/101‑1 (MQ=255)
                       ggCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCg                                                                          >  2:4866895/1‑101 (MQ=255)
                       ggCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCg                                                                          <  2:4866605/101‑1 (MQ=255)
                        gCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGt                                                                         <  1:4866547/101‑1 (MQ=255)
                        gCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGt                                                                         <  2:4866697/101‑1 (MQ=255)
                         cATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCt                                                                             >  2:4866900/1‑96 (MQ=255)
                         cATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGtc                                                                        >  1:4866899/1‑101 (MQ=255)
                          aTCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGtct                                                                       <  2:4866312/101‑1 (MQ=255)
                           tCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTGCGtctc                                                                      <  1:4866519/101‑1 (MQ=255)
                            cgcgCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCa                                                                     >  2:4866903/1‑101 (MQ=255)
                             gcgcATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCaa                                                                    >  2:4866907/1‑101 (MQ=255)
                              cgcATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCtt                                                                            >  1:4866908/1‑92 (MQ=255)
                               gcATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGtct                                                                       <  1:4866552/96‑1 (MQ=255)
                               gcATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACg                                                                  <  1:4866570/101‑1 (MQ=255)
                               gcATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACg                                                                  <  1:4866602/101‑1 (MQ=255)
                               gcATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACg                                                                  >  1:4866913/1‑101 (MQ=255)
                               gcATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACg                                                                  >  1:4866910/1‑101 (MQ=255)
                                cATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACgg                                                                 >  1:4866917/1‑101 (MQ=255)
                                cATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACgg                                                                 >  2:4866916/1‑101 (MQ=255)
                                 aTTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACgg                                                                 >  2:4866923/1‑100 (MQ=255)
                                 aTTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGc                                                                <  1:4866530/101‑1 (MQ=255)
                                 aTTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGc                                                                >  1:4866922/1‑101 (MQ=255)
                                 aTTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGc                                                                <  1:4866514/101‑1 (MQ=255)
                                  ttttGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCg                                                               <  1:4866418/101‑1 (MQ=255)
                                  ttttGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCg                                                               >  2:4866925/1‑101 (MQ=255)
                                   tttGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGa                                                              >  1:4866930/1‑101 (MQ=255)
                                   tttGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGa                                                              <  2:4866810/101‑1 (MQ=255)
                                    ttGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGAt                                                             >  2:4866931/1‑101 (MQ=255)
                                     tGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATc                                                            <  1:4866501/101‑1 (MQ=255)
                                        cACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGcc                                                         <  2:4866676/101‑1 (MQ=255)
                                        cACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGcc                                                         >  1:4866933/1‑101 (MQ=255)
                                           gCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGc                                                      >  2:4866937/1‑101 (MQ=255)
                                              gCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGcc                                                         >  2:4866941/1‑95 (MQ=255)
                                               cccTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCAtct                                                  >  1:4866945/1‑101 (MQ=255)
                                               cccTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCAtct                                                  >  1:4866943/1‑101 (MQ=255)
                                                ccTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCa                                                        >  1:4866948/1‑94 (MQ=255)
                                                   gCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTc                                              <  1:4866356/101‑1 (MQ=255)
                                                   gCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTc                                              <  1:4866442/101‑1 (MQ=255)
                                                   gCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTc                                              >  1:4866951/1‑101 (MQ=255)
                                                     gACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCgg                                            >  2:4866952/1‑101 (MQ=255)
                                                      aCATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGt                                           <  1:4866474/101‑1 (MQ=255)
                                                        aTCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCa                                         >  2:4866956/1‑101 (MQ=255)
                                                        aTCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCa                                         <  1:4866639/101‑1 (MQ=255)
                                                         tCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCAc                                        >  1:4866958/1‑101 (MQ=255)
                                                           cAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCg                                      >  1:4866962/1‑101 (MQ=255)
                                                            aGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGc                                     >  1:4866964/1‑101 (MQ=255)
                                                            aGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGc                                     >  2:4866965/1‑101 (MQ=255)
                                                             ggCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGcc                                    >  2:4866969/1‑101 (MQ=255)
                                                              gccgccTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCa                                   >  1:4866970/1‑101 (MQ=255)
                                                               ccgccTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCAt                                  >  1:4866973/1‑101 (MQ=255)
                                                                 gccTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCAtct                                >  1:4866976/1‑101 (MQ=255)
                                                                  ccTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCAtctc                               >  2:4866981/1‑101 (MQ=255)
                                                                  ccTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCAtctc                               >  1:4866979/1‑101 (MQ=255)
                                                                   cTTCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTcc                              <  2:4866487/101‑1 (MQ=255)
                                                                    ttCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCa                             <  2:4866561/101‑1 (MQ=255)
                                                                    ttCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCa                             >  2:4866985/1‑101 (MQ=255)
                                                                     tCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACgg                                                                 >  1:4866989/1‑64 (MQ=255)
                                                                     tCGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAg                            >  2:4866987/1‑101 (MQ=255)
                                                                      cGGTTGTTACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCAtctc                               >  1:4866993/1‑97 (MQ=255)
                                                                        gttgttACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGAtt                         >  2:4866995/1‑101 (MQ=255)
                                                                         ttgttACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTc                        >  1:4866996/1‑101 (MQ=255)
                                                                          tgttACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCa                       <  2:4866511/101‑1 (MQ=255)
                                                                          tgttACTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCa                       >  2:4866999/1‑101 (MQ=255)
                                                                               cTTCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCg                   <  2:4866550/100‑1 (MQ=255)
                                                                                 tCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGt                <  2:4866536/101‑1 (MQ=255)
                                                                                 tCCTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGt                >  2:4867003/1‑101 (MQ=255)
                                                                                  ccTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTa               >  1:4867004/1‑101 (MQ=255)
                                                                                   cTGACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCgg                  >  1:4867006/1‑97 (MQ=255)
                                                                                     gACGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATc             >  1:4867009/1‑100 (MQ=255)
                                                                                      aCGCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCa           <  1:4866625/101‑1 (MQ=255)
                                                                                        gCTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGc         <  1:4866582/101‑1 (MQ=255)
                                                                                         cTTTTCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGc         >  1:4867013/1‑100 (MQ=255)
                                                                                         cTTTTCCGGCACCAGGCAGCAAAAATGCGGCTTCGTCTCAACGGCGATCGCCAGCATCTCCTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCg        >  2:4867012/1‑101 (MQ=255)
                                                                                           tttCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTc      >  2:4867016/1‑101 (MQ=255)
                                                                                            ttCCGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCt     >  1:4867020/1‑101 (MQ=255)
                                                                                               cGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGAc  >  1:4867024/1‑101 (MQ=255)
                                                                                               cGGCACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGAc  <  2:4866480/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGAC  >  NC_000913/2701350‑2701545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A