Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F13 I1 R1 116 362.0 17214306 99.7% 17162663 98.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,749,662 C→T R297C (CGC→TGC)  yfjD → putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,749,6620CT99.7% 1625.8 / ‑5.0 382R297C (CGC→TGC) yfjDputative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (193/188);  total (194/188)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 385 total aligned reads displayed.

AAAAAGAGTTCACCAAAGAAACCATGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGCGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGATTT  >  NC_000913/2749564‑2749759
                                                                                                  |                                                                                                 
 aaaaGAGTTCACCAAAGAAACCATGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGca                                                                                                >  2:4953654/1‑101 (MQ=255)
 aaaaGAGTTCACCAAAGAAACCATGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGca                                                                                                >  2:4953657/1‑101 (MQ=255)
  aaaGAGTTCACCAAAGAAACCATGCTGCGCGCCGCGGAGGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGcaa                                                                                               >  1:4953660/1‑101 (MQ=255)
  aaaGAGTTCACCAAAGAAACCATGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGcaa                                                                                               <  1:4952914/101‑1 (MQ=255)
    agagTTCACCAAAGAAACCATGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGcaaca                                                                                             <  2:4953431/101‑1 (MQ=255)
     gagTTCACCAAAGAAACCATGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGcaacaa                                                                                            >  1:4953666/1‑101 (MQ=255)
     gagTTCACCAAAGAAACCATGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGcaacaa                                                                                            <  2:4953257/101‑1 (MQ=255)
     gagTTCACCAAAGAAACCATGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGcaacaa                                                                                            >  2:4953668/1‑101 (MQ=255)
      agTTCACCAAAGAAACCATGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACaaa                                                                                           <  1:4953059/101‑1 (MQ=255)
       gTTCACCAAAGAAACCATGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACaaaa                                                                                          >  1:4953676/1‑101 (MQ=255)
        ttCACCAAAGAAACCATGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACaaaaa                                                                                         <  2:4953225/101‑1 (MQ=255)
         tCACCAAAGAAACCATGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAaaag                                                                                        >  2:4953679/1‑101 (MQ=255)
         tCACCAAAGAAACCATGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAaaag                                                                                        <  2:4953193/101‑1 (MQ=255)
         tCACCAAAGAAACCATGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAaaag                                                                                        <  2:4953171/101‑1 (MQ=255)
           aCCAAAGAAACCATGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAaaagaa                                                                                      <  2:4953108/101‑1 (MQ=255)
            ccAAAGAAACCATGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAaaagaaa                                                                                     <  2:4953151/101‑1 (MQ=255)
            ccAAAGAAACCATGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAaaagaaa                                                                                     <  2:4953089/101‑1 (MQ=255)
            ccAAAGAAACCATGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAACACGCAGTTGGTAAAGTTTCAGTGCAACAAaaagaaa                                                                                     <  2:4953042/101‑1 (MQ=255)
             cAAAGAAACCATGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAaaagaaag                                                                                    >  1:4953685/1‑101 (MQ=255)
              aaaGAAACCATGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGt                                                                                   <  2:4953389/101‑1 (MQ=255)
               aaGAAACCATGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTc                                                                                  <  2:4952898/101‑1 (MQ=255)
               aaGAAACCATGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTc                                                                                  >  1:4953691/1‑101 (MQ=255)
               aaGAAACCATGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTc                                                                                  >  2:4953689/1‑101 (MQ=255)
                aGAAACCATGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCg                                                                                 >  1:4953699/1‑101 (MQ=255)
                aGAAACCATGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCg                                                                                 >  2:4953696/1‑101 (MQ=255)
                 gAAACCATGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCgg                                                                                >  2:4953703/1‑101 (MQ=255)
                   aaCCATGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGcc                                                                              >  1:4953708/1‑101 (MQ=255)
                    aCCATGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCt                                                                             <  1:4953140/101‑1 (MQ=255)
                       aTGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGgt                                                                          >  2:4953713/1‑101 (MQ=255)
                       aTGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGgt                                                                          >  1:4953712/1‑101 (MQ=255)
                        tgctgcGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGgtc                                                                         <  2:4952970/101‑1 (MQ=255)
                        tgctgcGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGgtc                                                                         <  2:4953231/101‑1 (MQ=255)
                         gctgcGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGgtcg                                                                        >  2:4953717/1‑101 (MQ=255)
                          ctgcGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGgtcgt                                                                       >  2:4953721/1‑101 (MQ=255)
                           tgcGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGgtcgtc                                                                      >  2:4953725/1‑101 (MQ=255)
                            gcgcgcCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCa                                                                     <  2:4953313/101‑1 (MQ=255)
                             cgcgcCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCaa                                                                    <  1:4953398/101‑1 (MQ=255)
                             cgcgcCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCaa                                                                    <  1:4952819/101‑1 (MQ=255)
                               cgccgcGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACg                                                                  >  1:4953731/1‑101 (MQ=255)
                                 ccgcGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAg                                                                >  2:4953732/1‑101 (MQ=255)
                                 ccgcGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAg                                                                <  2:4953133/101‑1 (MQ=255)
                                  cgcgGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGt                                                               <  1:4953302/101‑1 (MQ=255)
                                  cgcgGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGt                                                               <  1:4953251/101‑1 (MQ=255)
                                     ggACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGCTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATg                                                            >  2:4953739/1‑101 (MQ=255)
                                      gACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTc                                                                                  <  1:4953241/78‑1 (MQ=255)
                                        cGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGgag                                                         >  1:4953743/1‑101 (MQ=255)
                                        cGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGgag                                                         <  1:4953176/101‑1 (MQ=255)
                                         gagaTCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGgaga                                                        <  2:4953052/101‑1 (MQ=255)
                                         gagaTCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGgaga                                                        <  2:4953499/101‑1 (MQ=255)
                                          agaTCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGAc                                                       >  2:4953746/1‑101 (MQ=255)
                                           gaTCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACa                                                      >  1:4953750/1‑101 (MQ=255)
                                            aTCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACAt                                                     >  2:4953753/1‑101 (MQ=255)
                                             tCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACAtt                                                    <  1:4953381/101‑1 (MQ=255)
                                              cTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTc                                                   <  1:4953114/101‑1 (MQ=255)
                                                aTTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAg                                                 >  1:4953760/1‑101 (MQ=255)
                                                aTTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAg                                                 >  2:4953761/1‑101 (MQ=255)
                                                aTTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAg                                                 <  2:4953322/101‑1 (MQ=255)
                                                 ttttGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAgg                                                <  1:4953472/101‑1 (MQ=255)
                                                 ttttGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAgg                                                <  1:4953326/101‑1 (MQ=255)
                                                  tttGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAggg                                               <  1:4953444/101‑1 (MQ=255)
                                                      tgCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTg                                           >  2:4953766/1‑101 (MQ=255)
                                                        ccGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCt                                            >  1:4953770/1‑98 (MQ=255)
                                                          ggAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGa                                       <  1:4953073/101‑1 (MQ=255)
                                                          ggAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGa                                       >  1:4953774/1‑101 (MQ=255)
                                                           gAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGAc                                      >  2:4953778/1‑101 (MQ=255)
                                                           gAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGAc                                      <  1:4953043/101‑1 (MQ=255)
                                                           gAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGAc                                      <  1:4953374/101‑1 (MQ=255)
                                                             aGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACgg                                    >  1:4953783/1‑101 (MQ=255)
                                                              ggTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGt                                   <  2:4953021/101‑1 (MQ=255)
                                                               gTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGtt                                  <  2:4953352/101‑1 (MQ=255)
                                                                tACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTg                                 >  2:4953786/1‑101 (MQ=255)
                                                                 aCGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGa                                <  1:4953276/101‑1 (MQ=255)
                                                                  cgccgcTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGaa                               >  1:4953792/1‑101 (MQ=255)
                                                                  cgccgcTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGaa                               >  2:4953791/1‑101 (MQ=255)
                                                                     cgcTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGat                            >  2:4953799/1‑101 (MQ=255)
                                                                      gcTCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGata                           >  1:4953800/1‑101 (MQ=255)
                                                                        tCAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATAtt                         <  1:4953527/101‑1 (MQ=255)
                                                                         cAGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTc                        >  2:4953804/1‑101 (MQ=255)
                                                                          aGCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCt                       >  1:4953807/1‑101 (MQ=255)
                                                                           gCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTg                      >  1:4953812/1‑101 (MQ=255)
                                                                           gCACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTg                      <  1:4953413/101‑1 (MQ=255)
                                                                            cACGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTgg                     <  1:4953221/101‑1 (MQ=255)
                                                                             aCGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGa                    >  2:4953815/1‑101 (MQ=255)
                                                                              cGCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGaa                   <  1:4953094/101‑1 (MQ=255)
                                                                               gCAGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAag                  <  2:4953273/101‑1 (MQ=255)
                                                                                 aGTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGgtc                                                                         >  1:4953821/1‑44 (MQ=255)
                                                                                  gTTGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAgaga               >  2:4953823/1‑101 (MQ=255)
                                                                                   ttGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGAt              <  1:4953292/101‑1 (MQ=255)
                                                                                   ttGGTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGAt              >  2:4953828/1‑101 (MQ=255)
                                                                                     ggTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTg            <  1:4953336/101‑1 (MQ=255)
                                                                                     ggTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTg            >  1:4953831/1‑101 (MQ=255)
                                                                                      gTAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGAt              <  2:4953371/98‑1 (MQ=255)
                                                                                       tAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTc          >  2:4953837/1‑101 (MQ=255)
                                                                                       tAAAGTTTCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTc          >  1:4953838/1‑101 (MQ=255)
                                                                                            tttCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAag                  >  2:4953846/1‑88 (MQ=255)
                                                                                            tttCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGa     >  1:4953845/1‑101 (MQ=255)
                                                                                             ttCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGAt    <  1:4953582/101‑1 (MQ=255)
                                                                                             ttCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGAt    <  1:4953194/101‑1 (MQ=255)
                                                                                              tCAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGAtt   <  1:4953262/101‑1 (MQ=255)
                                                                                               cAGTGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGAttt  <  1:4953163/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
AAAAAGAGTTCACCAAAGAAACCATGCTGCGCGCCGCGGACGAGATCTATTTTGTGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGCGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGATTT  >  NC_000913/2749564‑2749759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A