Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F13 I1 R1 108 324.7 15372274 99.7% 15326157 98.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,316,298 T→C L41P (CTG→CCG)  rcsB → DNA‑binding transcriptional activator RcsB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,316,2980TC100.0% 1262.8 / NA 304L41P (CTG→CCG) rcsBDNA‑binding transcriptional activator RcsB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (157/147);  total (157/147)

Only 100 of 304 total aligned reads displayed.

GCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTAC  >  NC_000913/2316201‑2316395
                                                                                                 |                                                                                                 
gCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGAt                                                                                                <  1:3774371/101‑1 (MQ=255)
gCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGAt                                                                                                >  2:3774943/1‑101 (MQ=255)
gCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGAt                                                                                                <  2:3774576/101‑1 (MQ=255)
 ccGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATc                                                                                               <  2:3774795/101‑1 (MQ=255)
  cGATGACCATCCGATAGTCTTGTGCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGAt                                                                                                <  2:3774306/99‑1 (MQ=255)
   gATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATcaa                                                                                             >  1:3774947/1‑101 (MQ=255)
    aTGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATcaac                                                                                            >  2:3774950/1‑101 (MQ=255)
    aTGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATcaac                                                                                            <  1:3774496/101‑1 (MQ=255)
    aTGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATcaac                                                                                            <  2:3774270/101‑1 (MQ=255)
     tGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATcaaca                                                                                           <  1:3774343/101‑1 (MQ=255)
      gACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATcaacaa                                                                                          >  2:3774955/1‑101 (MQ=255)
      gACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATcaacaa                                                                                          <  2:3774671/101‑1 (MQ=255)
       aCCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCaacaac                                                                                         <  1:3774459/101‑1 (MQ=255)
         cATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCt                                                                                       <  1:3774202/101‑1 (MQ=255)
          aTCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTg                                                                                      >  1:3774961/1‑101 (MQ=255)
           tCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGc                                                                                     >  1:3774964/1‑101 (MQ=255)
            ccGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGcc                                                                                    >  2:3774968/1‑101 (MQ=255)
             cGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCg                                                                                   <  1:3774522/101‑1 (MQ=255)
               aTAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGaa                                                                                 >  1:3774971/1‑101 (MQ=255)
               aTAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGaa                                                                                 >  2:3774972/1‑101 (MQ=255)
                tAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGaaa                                                                                >  1:3774975/1‑101 (MQ=255)
                tAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGaaa                                                                                <  1:3774443/101‑1 (MQ=255)
                tAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGaaa                                                                                <  1:3774836/101‑1 (MQ=255)
                  gTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACt                                                                              <  2:3774428/101‑1 (MQ=255)
                   tCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCt                                                                                       >  1:3774980/1‑91 (MQ=255)
                   tCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTg                                                                             <  2:3774453/101‑1 (MQ=255)
                   tCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTg                                                                             >  2:3774979/1‑101 (MQ=255)
                      tGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGAt                                                                          >  2:3774983/1‑101 (MQ=255)
                         tCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATgcg                                                                       >  1:3774988/1‑101 (MQ=255)
                         tCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATgcg                                                                       >  2:3774989/1‑101 (MQ=255)
                            gTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCAt                                                                    <  1:3774630/101‑1 (MQ=255)
                             tATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCAtg                                                                   >  1:3774994/1‑101 (MQ=255)
                             tATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCAtg                                                                   >  2:3774996/1‑101 (MQ=255)
                               ttCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCAtgtg                                                                 <  1:3774076/101‑1 (MQ=255)
                                 cGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGtt                                                               <  1:3774382/101‑1 (MQ=255)
                                 cGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGtt                                                               >  1:3775000/1‑101 (MQ=255)
                                 cGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGtt                                                               >  2:3775001/1‑101 (MQ=255)
                                   cAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGa                                                             >  1:3775009/1‑101 (MQ=255)
                                   cAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGa                                                             >  1:3775006/1‑101 (MQ=255)
                                    aaaTCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGAt                                                            >  2:3775010/1‑101 (MQ=255)
                                     aaTCACTTGAGCAAATTGAGTGGGTGAATTTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGAtt                                                           <  2:3774595/101‑1 (MQ=255)
                                     aaTCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGAtt                                                           >  2:3775014/1‑101 (MQ=255)
                                      aTCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTa                                                          >  1:3775018/1‑101 (MQ=255)
                                      aTCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTa                                                          <  1:3774594/101‑1 (MQ=255)
                                       tCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTAc                                                         >  2:3775020/1‑101 (MQ=255)
                                         aCTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCg                                                       >  2:3775025/1‑101 (MQ=255)
                                          cTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGa                                                      >  1:3775026/1‑101 (MQ=255)
                                          cTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGa                                                      <  1:3774754/101‑1 (MQ=255)
                                           ttGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGAt                                                     <  2:3774493/101‑1 (MQ=255)
                                            tGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGAtc                                                    >  2:3775029/1‑101 (MQ=255)
                                             gAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGAtct                                                   <  1:3774537/101‑1 (MQ=255)
                                               gCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGAtctct                                                 >  2:3775033/1‑101 (MQ=255)
                                                cAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGAtctctc                                                >  1:3775035/1‑101 (MQ=255)
                                                 aaaTTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTcc                                               <  1:3774578/101‑1 (MQ=255)
                                                  aaTTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCa                                              <  1:3774551/101‑1 (MQ=255)
                                                  aaTTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCa                                              >  1:3775040/1‑101 (MQ=255)
                                                    ttGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATg                                            <  2:3774738/101‑1 (MQ=255)
                                                      gAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGcc                                          <  2:3774345/101‑1 (MQ=255)
                                                       aGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCt                                         <  1:3774909/101‑1 (MQ=255)
                                                       aGTGGGTGAATGTTGTAGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCt                                         <  1:3774568/101‑1 (MQ=255)
                                                        gTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTg                                        <  2:3774622/101‑1 (MQ=255)
                                                        gTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTg                                        >  1:3775044/1‑101 (MQ=255)
                                                         tGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTgg                                       >  2:3775045/1‑101 (MQ=255)
                                                           ggTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCg                                     >  2:3775049/1‑101 (MQ=255)
                                                            gTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGa                                    <  2:3774603/101‑1 (MQ=255)
                                                              gAATGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATa                                  >  1:3775052/1‑101 (MQ=255)
                                                               aaTGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATaa                                 <  2:3774698/101‑1 (MQ=255)
                                                               aaTGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATaa                                 <  2:3774661/101‑1 (MQ=255)
                                                                aTGTTGTCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAg                                <  2:3774477/101‑1 (MQ=255)
                                                                 tgttgtCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGt                               >  1:3775056/1‑101 (MQ=255)
                                                                    tgtCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGCTAAGTACg                            >  2:3775057/1‑101 (MQ=255)
                                                                     gtCGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACgg                           <  2:3774510/101‑1 (MQ=255)
                                                                       cGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACg                            >  2:3775060/1‑98 (MQ=255)
                                                                       cGGCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCg                         <  2:3774559/101‑1 (MQ=255)
                                                                        ggCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGaaa                                                                                <  2:3774837/45‑1 (MQ=255)
                                                                        ggCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGa                        >  1:3775064/1‑101 (MQ=255)
                                                                         gCGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGAt                       <  1:3774426/101‑1 (MQ=255)
                                                                          cGAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAAc                                                                               >  1:3775068/1‑44 (MQ=255)
                                                                           gAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATTcc                                          >  1:3775072/1‑80 (MQ=255)
                                                                           gAATTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATgg                     >  2:3775070/1‑101 (MQ=255)
                                                                             aTTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCa                   >  1:3775077/1‑101 (MQ=255)
                                                                             aTTTGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTAGCGATAAGTACGGCGATGGCa                   >  2:3775078/1‑101 (MQ=255)
                                                                               ttGAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCAtt                 <  2:3774636/101‑1 (MQ=255)
                                                                                 gAAGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTAc               >  1:3775082/1‑101 (MQ=255)
                                                                                   aGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCt             >  2:3775086/1‑101 (MQ=255)
                                                                                   aGACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCt             <  1:3774600/101‑1 (MQ=255)
                                                                                    gACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGAt                                                            <  1:3774649/53‑1 (MQ=255)
                                                                                    gACTCTACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCtt            <  1:3774474/101‑1 (MQ=255)
                                                                                      ctctACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTaa          >  2:3775089/1‑101 (MQ=255)
                                                                                      ctctACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTaa          <  2:3774532/101‑1 (MQ=255)
                                                                                      ctctACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTaa          >  1:3775090/1‑101 (MQ=255)
                                                                                        ctACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATc        >  2:3775093/1‑101 (MQ=255)
                                                                                        cgaCAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCa                   <  2:3774407/88‑1 (MQ=255)
                                                                                         tACAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCa       <  1:3774557/101‑1 (MQ=255)
                                                                                          aCAGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCaa      <  1:3774670/101‑1 (MQ=255)
                                                                                           cAGCACCGATCAACAACCGGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATg                                            <  2:3774521/62‑1 (MQ=255)
                                                                                            aGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGAtctc                                                  >  2:3775100/1‑55 (MQ=255)
                                                                                            aGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGt    <  2:3774583/101‑1 (MQ=255)
                                                                                            aGCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGt    >  1:3775096/1‑101 (MQ=255)
                                                                                             gCACCGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGAtct                                                   >  1:3775103/1‑53 (MQ=255)
                                                                                                 |                                                                                                 
GCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTAC  >  NC_000913/2316201‑2316395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A