Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F13 I1 R1	114	305.6	14428468	99.7%	14385182	98.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,951,417	G→A	R349C (CGC→TGC)	recD ←	exodeoxyribonuclease V subunit RecD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,951,417	0	G	A	97.5%	969.4 / 6.8	240	R349C (CGC→TGC)	recD	exodeoxyribonuclease V subunit RecD
Reads supporting (aligned to +/- strand): ref base G (2/4); new base A (132/102); total (134/106)
Fisher's exact test for biased strand distribution p-value = 4.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 246 total aligned reads displayed.

TGCCGTTTTATCACCACGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCA  >  NC_000913/2951319‑2951514

tGCCGTTTTATCACCACGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGcaca                                                                                                 >  1:4481437/1‑101 (MQ=255)

tGCCGTTTTATCACCACGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGcaca                                                                                                 >  2:4481436/1‑101 (MQ=255)

 gCCGTTTTATCACCACGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACaa                                                                                                >  1:4481439/1‑101 (MQ=255)

 gCCGTTTTATCACCACGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACaa                                                                                                <  1:4481129/101‑1 (MQ=255)

 gCCGTTTTATCACCACGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACaa                                                                                                >  2:4481441/1‑101 (MQ=255)

  ccGTTTTATCACCACGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACaaa                                                                                               <  1:4480998/101‑1 (MQ=255)

  ccGTTTTATCACCACGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACaaa                                                                                               >  2:4481444/1‑101 (MQ=255)

  ccGTTTTACCACCACGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACaaa                                                                                               <  1:4480869/101‑1 (MQ=255)

    gTTTTATCACCACGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGa                                                                                             >  1:4481447/1‑101 (MQ=255)

     ttttATCACCACGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGAt                                                                                            >  2:4481450/1‑101 (MQ=255)

     ttttATCACCACGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGAt                                                                                            <  2:4480937/101‑1 (MQ=255)

      tttATCACCACGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATg                                                                                           <  1:4480969/101‑1 (MQ=255)

        tATCACCACGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGcc                                                                                         >  1:4481452/1‑101 (MQ=255)

          tCACCACGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGc                                                                                       >  1:4481455/1‑101 (MQ=255)

           caccacGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCt                                                                                      <  2:4481067/101‑1 (MQ=255)

            accacGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCtt                                                                                     <  1:4481043/101‑1 (MQ=255)

            accacGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCtt                                                                                     <  1:4480935/101‑1 (MQ=255)

            accacGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCtt                                                                                     <  2:4481020/101‑1 (MQ=255)

              cacGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCGGGCAGAGACTGTCGCAGAAAGATGCCGCTTCt                                                                                   >  2:4481460/1‑101 (MQ=255)

              cacGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCt                                                                                   >  1:4481458/1‑101 (MQ=255)

               acGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCtg                                                                                  >  2:4481463/1‑101 (MQ=255)

               acGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCtg                                                                                  >  1:4481464/1‑101 (MQ=255)

                   ttGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGcc                                                                              >  1:4481467/1‑101 (MQ=255)

                    tGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCg                                                                             >  1:4481469/1‑101 (MQ=255)

                     gATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCtg                                                                                  <  2:4480883/95‑1 (MQ=255)

                        cgccgcAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCtgtg                                                                                >  1:4481474/1‑94 (MQ=255)

                        cgccgcAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTc                                                                         <  2:4480747/101‑1 (MQ=255)

                          ccgcAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCt                                                                       <  1:4481183/101‑1 (MQ=255)

                          ccgcAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCt                                                                       <  2:4481035/101‑1 (MQ=255)

                          ccgcAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCt                                                                       <  1:4481006/101‑1 (MQ=255)

                           cgcAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCtt                                                                                     >  2:4481476/1‑86 (MQ=255)

                            gcAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGc                                                                     >  2:4481478/1‑101 (MQ=255)

                             cAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGcc                                                                    >  1:4481479/1‑101 (MQ=255)

                               gCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCgg                                                                  >  1:4481484/1‑101 (MQ=255)

                                cTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGa                                                                 <  2:4481212/101‑1 (MQ=255)

                                cTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGa                                                                 <  2:4481107/101‑1 (MQ=255)

                                cTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGa                                                                 >  2:4481485/1‑101 (MQ=255)

                                 tAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGaa                                                                >  1:4481487/1‑101 (MQ=255)

                                  aaCTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCgg                                                                  <  1:4481038/98‑1 (MQ=255)

                                  aaCTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAAc                                                               >  1:4481492/1‑101 (MQ=255)

                                  aaCTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAAc                                                               >  2:4481491/1‑101 (MQ=255)

                                     tGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTg                                                            >  1:4481495/1‑101 (MQ=255)

                                        ccAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGt                                                         >  1:4481498/1‑101 (MQ=255)

                                         cAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTc                                                        <  1:4481150/101‑1 (MQ=255)

                                         cAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTc                                                        >  2:4481502/1‑101 (MQ=255)

                                         cAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTc                                                        >  2:4481499/1‑101 (MQ=255)

                                           aTGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTccc                                                      >  2:4481504/1‑101 (MQ=255)

                                              ccAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGt                                                   <  1:4480993/101‑1 (MQ=255)

                                               cAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTc                                                  >  2:4481508/1‑101 (MQ=255)

                                               cAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTc                                                  <  1:4480959/101‑1 (MQ=255)

                                               cAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTc                                                  <  1:4481057/101‑1 (MQ=255)

                                                aGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCa                                                 >  1:4481509/1‑101 (MQ=255)

                                                 gAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAg                                                <  2:4481138/101‑1 (MQ=255)

                                                  aaTCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAgg                                               <  1:4480699/101‑1 (MQ=255)

                                                  aaTCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAgg                                               >  2:4481515/1‑101 (MQ=255)

                                                  aaTCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAgg                                               >  1:4481512/1‑101 (MQ=255)

                                                    tCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAggcg                                             >  1:4481517/1‑101 (MQ=255)

                                                      gCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAggcggc                                           >  1:4481519/1‑101 (MQ=255)

                                                      gCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAggcggc                                           <  2:4481101/101‑1 (MQ=255)

                                                        tGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCtt                                         >  2:4481522/1‑101 (MQ=255)

                                                         gCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTa                                        <  2:4481088/101‑1 (MQ=255)

                                                          ccGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAg                                       >  2:4481524/1‑101 (MQ=255)

                                                           cGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGc                                      >  1:4481526/1‑101 (MQ=255)

                                                            gAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAg                                                <  1:4481097/90‑1 (MQ=255)

                                                            gAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCt                                     <  2:4480980/101‑1 (MQ=255)

                                                             aaaCGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTg                                    >  1:4481528/1‑101 (MQ=255)

                                                               aCGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGcc                                  >  1:4481533/1‑101 (MQ=255)

                                                                cGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCt                                 <  2:4481194/101‑1 (MQ=255)

                                                                 gATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTg                                <  2:4481311/101‑1 (MQ=255)

                                                                 gATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTg                                >  1:4481535/1‑101 (MQ=255)

                                                                   tAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCt                                 <  1:4481086/98‑1 (MQ=255)

                                                                   tAGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGc                              >  1:4481538/1‑101 (MQ=255)

                                                                    aGCTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCa                             <  2:4481120/101‑1 (MQ=255)

                                                                      cTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACg                           <  1:4481072/101‑1 (MQ=255)

                                                                      cTTTTTTGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACg                           >  1:4481540/1‑101 (MQ=255)

                                                                       ttttttGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGc                          >  1:4481543/1‑101 (MQ=255)

                                                                        tttttGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCGCCGTCAGGCGGATTAGCTGCCTGGCAc                            >  2:4481544/1‑98 (MQ=255)

                                                                        tttttGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACg                           <  2:4481059/99‑1 (MQ=255)

                                                                         ttttGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTc                        <  2:4481026/101‑1 (MQ=255)

                                                                          tttGCAGCAGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTcg                       >  1:4481546/1‑101 (MQ=255)

                                                                             gcagcaGGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGt                                                             >  2:4481548/1‑60 (MQ=255)

                                                                              cagcagGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTcggcgg                   >  1:4481550/1‑101 (MQ=255)

                                                                              cagcagGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTcggcgg                   <  1:4481195/101‑1 (MQ=255)

                                                                               agcagGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGt                  <  1:4481074/101‑1 (MQ=255)

                                                                               agcagGCAGAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGt                  <  1:4481291/101‑1 (MQ=255)

                                                                                 caggcagAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTaa                >  1:4481555/1‑101 (MQ=255)

                                                                                  aggcagAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTaaa               <  2:4481167/101‑1 (MQ=255)

                                                                                  aggcagAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTaaa               >  2:4481556/1‑101 (MQ=255)

                                                                                  aggcagAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTaaa               <  1:4480989/101‑1 (MQ=255)

                                                                                    gcagAGACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGc             >  2:4481558/1‑101 (MQ=255)

                                                                                      agagACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGccc           <  1:4481253/101‑1 (MQ=255)

                                                                                      agagACTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGccc           >  1:4481562/1‑101 (MQ=255)

                                                                                        agaCTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCcgc         >  1:4481566/1‑101 (MQ=255)

                                                                                        agaCTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCcgc         >  2:4481565/1‑101 (MQ=255)

                                                                                         gaCTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTg                                    >  2:4481571/1‑73 (MQ=255)

                                                                                         gaCTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCcgcg        >  1:4481569/1‑101 (MQ=255)

                                                                                          aCTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGt       <  1:4481234/101‑1 (MQ=255)

                                                                                          aCTGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGt       <  2:4481285/101‑1 (MQ=255)

                                                                                            tGTCGCACAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTg     >  1:4481573/1‑101 (MQ=255)

                                                                                             gTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTgg    <  1:4481318/101‑1 (MQ=255)

TGCCGTTTTATCACCACGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCA  >  NC_000913/2951319‑2951514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A