Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F13 I1 R1	114	305.6	14428468	99.7%	14385182	98.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,961,288	T→C	S381G (AGC→GGC)	recC ←	exodeoxyribonuclease V subunit RecC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,961,288	0	T	C	99.7%	1196.0 / NA	285	S381G (AGC→GGC)	recC	exodeoxyribonuclease V subunit RecC
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (130/154); total (131/154)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 285 total aligned reads displayed.

GCCACCATCACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCTATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCTACGGGAAAACTCTTCGATGTTCACACCAGCAACGGCG  >  NC_000913/2961191‑2961385

  caccaTCACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGc                                                                                              <  2:4496809/101‑1 (MQ=255)

   accaTCACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCa                                                                                             >  1:4497000/1‑101 (MQ=255)

   accaTCACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCa                                                                                             <  1:4496746/101‑1 (MQ=255)

    ccaTCACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCaa                                                                                            >  2:4497003/1‑101 (MQ=255)

        cACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGt                                                                                        >  2:4497006/1‑101 (MQ=255)

         aCGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTg                                                                                       >  1:3609223/1‑101 (MQ=255)

          cGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTgg                                                                                      <  1:4496574/101‑1 (MQ=255)

           gatgatGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGa                                                                                     >  1:4497008/1‑101 (MQ=255)

           gatgatGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGa                                                                                     <  2:4496505/101‑1 (MQ=255)

            atgatgTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGaa                                                                                    >  2:4497012/1‑101 (MQ=255)

            atgatgTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGaa                                                                                    <  1:4496857/101‑1 (MQ=255)

              gatgTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAgg                                                                                  >  1:4497013/1‑101 (MQ=255)

               atgTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGt                                                                                 <  2:4496636/101‑1 (MQ=255)

               atgTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGt                                                                                 <  2:4496543/101‑1 (MQ=255)

                 gTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAgg                                                                                  >  2:4497017/1‑98 (MQ=255)

                 gTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGa                                                                               <  1:4496682/101‑1 (MQ=255)

                   cgcgcgGAGTAAGTGTCGGTTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGaa                                                                                    <  2:4496651/94‑1 (MQ=255)

                   cgcgcgGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGt                                                                                 <  2:4496748/97‑1 (MQ=255)

                     cgcgGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACt                                                                           <  1:4496514/101‑1 (MQ=255)

                      gcgGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATa                                                                             >  2:4497021/1‑98 (MQ=255)

                      gcgGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTg                                                                          <  1:4496649/101‑1 (MQ=255)

                      gcgGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTg                                                                          <  1:4496624/101‑1 (MQ=255)

                         gAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTa                                                                       >  2:4497024/1‑101 (MQ=255)

                         gAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTa                                                                       >  1:4497025/1‑101 (MQ=255)

                            tAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATcc                                                                    <  1:4496552/101‑1 (MQ=255)

                            tAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATcc                                                                    <  2:4496832/101‑1 (MQ=255)

                             aaGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCa                                                                   <  2:4496471/101‑1 (MQ=255)

                              aGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAg                                                                  >  1:4497029/1‑101 (MQ=255)

                                tgtCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGt                                                                 >  2:4497030/1‑100 (MQ=255)

                                tgtCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTg                                                                <  1:4496352/101‑1 (MQ=255)

                                 gtCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTgg                                                               <  2:4496522/101‑1 (MQ=255)

                                   cGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGt                                                                                 <  2:4496579/81‑1 (MQ=255)

                                    gggTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATc                                                            >  2:4497033/1‑101 (MQ=255)

                                     ggTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCa                                                           <  2:4496618/101‑1 (MQ=255)

                                       tCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGGGa                                                                               >  2:4497038/1‑79 (MQ=255)

                                        cTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGc                                                        <  1:4496712/101‑1 (MQ=255)

                                           cctccAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATc                                                            <  2:4496412/94‑1 (MQ=255)

                                           cctccAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCa                                                           <  2:4496591/95‑1 (MQ=255)

                                           cctccAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCggg                                                     >  2:4497041/1‑101 (MQ=255)

                                            ctccAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGc                                                    <  1:4496619/101‑1 (MQ=255)

                                            ctccAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGc                                                    <  1:4496549/101‑1 (MQ=255)

                                             tccAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCg                                                   >  1:4497044/1‑101 (MQ=255)

                                              ccAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGt                                                  <  2:4496671/101‑1 (MQ=255)

                                              ccAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGt                                                  >  2:4497046/1‑101 (MQ=255)

                                               cAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGtt                                                 <  1:4496589/101‑1 (MQ=255)

                                                aGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGttt                                                <  1:4496966/101‑1 (MQ=255)

                                                aGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGttt                                                <  2:4496604/101‑1 (MQ=255)

                                                 gCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTg                                               <  2:4496266/101‑1 (MQ=255)

                                                 gCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTg                                               <  1:4496445/101‑1 (MQ=255)

                                                   aTCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGtt                                             <  1:4496698/101‑1 (MQ=255)

                                                   aTCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGtt                                             <  1:4496599/101‑1 (MQ=255)

                                                   aTCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGtt                                             <  1:4496654/101‑1 (MQ=255)

                                                   aTCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGtt                                             <  2:4496502/101‑1 (MQ=255)

                                                   aTCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGtt                                             >  2:4497050/1‑101 (MQ=255)

                                                   aTCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGtt                                             >  1:4497051/1‑101 (MQ=255)

                                                   aTCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGtt                                             <  1:4496735/101‑1 (MQ=255)

                                                   aTCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGtt                                             >  2:4497053/1‑101 (MQ=255)

                                                     cGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTAt                                           <  2:4496627/101‑1 (MQ=255)

                                                     cGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTAt                                           <  1:4496836/101‑1 (MQ=255)

                                                      gCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATc                                          <  1:4496630/101‑1 (MQ=255)

                                                      gCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATc                                          >  1:4497057/1‑101 (MQ=255)

                                                       ccAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCg                                         <  1:4496787/101‑1 (MQ=255)

                                                         agcagGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCt                                       <  2:4496534/101‑1 (MQ=255)

                                                         agcagGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCt                                       >  2:4497060/1‑101 (MQ=255)

                                                          gcagGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCTa                                      >  2:4497063/1‑101 (MQ=255)

                                                            agGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCTACg                                    >  2:4497066/1‑101 (MQ=255)

                                                            agGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCTACg                                    <  1:4496501/101‑1 (MQ=255)

                                                              gCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCTACggg                                  <  1:4496611/101‑1 (MQ=255)

                                                               cGATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCTACGGGa                                 >  1:4497068/1‑101 (MQ=255)

                                                                gATCGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCGGTGGATCAAGCGGGCGTTTGTTATCGCTACGGGaa                                <  2:4496566/101‑1 (MQ=255)

                                                                   cGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCTACGGGAAAAc                             >  1:4497073/1‑101 (MQ=255)

                                                                   cGTGTAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCTACGGGAAAAc                             >  2:4497074/1‑101 (MQ=255)

                                                                    gtgtAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCTACGGGAAAAct                            >  2:4497077/1‑101 (MQ=255)

                                                                     tgtAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCTACGGGAAAActc                           <  1:4496525/101‑1 (MQ=255)

                                                                     tgtAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCTACGGGAAAActc                           <  1:4496772/101‑1 (MQ=255)

                                                                      gtAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCTACGGGAAAActct                          <  1:4496724/101‑1 (MQ=255)

                                                                       tAAAACTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCTACGGGAAAACTCtt                         >  1:4497080/1‑101 (MQ=255)

                                                                        aaaaCTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCTACGGGAAAACTCTTc                        >  1:4497083/1‑101 (MQ=255)

                                                                         aaaCTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCTACGGGAAAACTCTTCg                       >  2:4497086/1‑101 (MQ=255)

                                                                         aaaCTTCAACTTCACGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCTACGGGAAAACTCTTCg                       >  1:4497088/1‑101 (MQ=255)

                                                                           acttcaacttcaCGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCTACGGGAAAACTCTTCGAt                     <  2:4496867/101‑1 (MQ=255)

                                                                            cttcaacttcaCGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCTACGGGAAAACTCTTCGATg                    >  1:4497091/1‑101 (MQ=255)

                                                                              tcaacttcaCGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCTACGGGAAAACTCTTCGATGtt                  >  1:4497094/1‑101 (MQ=255)

                                                                               caacttcaCGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCTACGGGAAAACTCTTCGATGTTc                 >  2:4497098/1‑101 (MQ=255)

                                                                                aacttcaCGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCTACGGGAAAACTCTTCGATGTTca                >  1:4497100/1‑101 (MQ=255)

                                                                                     caCGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGt                                                  >  1:4497105/1‑62 (MQ=255)

                                                                                     caCGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCTACGGGAAAACTCTTCGATGTTCACAcc            >  2:4497104/1‑100 (MQ=255)

                                                                                     caCGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCTACGGGAAAACTCTTCGATGTTCACACCa           <  1:4496707/101‑1 (MQ=255)

                                                                                     caCGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCTACGGGAAAACTCTTCGATGTTCACACCa           <  2:4496688/101‑1 (MQ=255)

                                                                                       cGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCTACGGGAAAACTCTTCGATGTTCACACCAGc         <  2:4496757/101‑1 (MQ=255)

                                                                                       cGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCTACGGGAAAACTCTTCGATGTTCACACCAGc         >  1:4497109/1‑101 (MQ=255)

                                                                                       cGCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCTACGGGAAAACTCTTCGATGTTCACACCAGc         <  1:4496676/101‑1 (MQ=255)

                                                                                        gCTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCTACGGGAAAACTCtt                         <  2:4496383/84‑1 (MQ=255)

                                                                                        gCTGCGGGCCATGGCAAACGCGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCTACGGGAAAAct                            >  2:4497110/1‑81 (MQ=255)

                                                                                         cTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCTACGGGAAAACTCTTCGATGTTCACACCAGCaa       <  2:4496558/101‑1 (MQ=255)

                                                                                         cTGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCTACGGGAAAACTCTTCGATGTTCACACCAGCaa       >  1:4497115/1‑101 (MQ=255)

                                                                                          tGCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCTACGGGAAAACTCTTCGATGTTCACACCAGCAAc      >  2:4497116/1‑101 (MQ=255)

                                                                                           gCGGGCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCTACGGGAAAACTCTTCGATGTTCACACCAGCAACg     >  2:4497120/1‑101 (MQ=255)

                                                                                             gggCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCTACGGGAAAACTCTTCGATGTTCacac             <  2:4496736/91‑1 (MQ=255)

                                                                                             gggCCATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCTACGGGAAAACTCTTCGATGTTCACACCAGCAACGgc   >  2:4497124/1‑101 (MQ=255)

GCCACCATCACGATGATGTCGCGCGGAGTAAGTGTCGGGTCTTCCTCCAGCATCGCCAGCAGGCGATCGTGTAAAACTTCAACTTCACGCTGCGGGCTATGGCAAACGTGGAAGGTGATACTGCTATCCAGTGGATCAAGCGGGCGTTTGTTATCGCTACGGGAAAACTCTTCGATGTTCACACCAGCAACGGCG  >  NC_000913/2961191‑2961385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A