Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F27 I0 R1 118 640.9 20101374 98.9% 19880258 149.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,482,325 G→A 100% R346H (CGC→CAC)  yheS → ABC‑F family protein predicted regulatory ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,482,3250GA100.0% 2563.2 / NA 674R346H (CGC→CAC) yheSABC‑F family protein predicted regulatory ATPase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (314/360);  total (314/360)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 679 total aligned reads displayed.

CGTTCCGCTTTAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCGCAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAATCACCTA  >  NC_000913/3482178‑3482471
                                                                                                                                                   |                                                                                                                                                  
  ttCCGCTTTAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCTTTACTGAAGAAGGAAAAAGTCAGCGCGGGCTCTGTCGATCTCATTAATCACGACTCGAATAAACTGAACCTGGTGCCCGGCTCGCGAATTGGTCAGTTAGGCcacact                                                                                                                                                >  2:5796858/1‑148 (MQ=255)
  ttCCGCTTTAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAAt                                                                                                                                                >  1:7975523/1‑150 (MQ=255)
      gCTTTAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGgcg                                                                                                                                            >  1:1278978/1‑150 (MQ=255)
       cTTTAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGgcgc                                                                                                                                           >  2:3139581/1‑150 (MQ=255)
        tttAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGcgcg                                                                                                                                          <  2:1163186/150‑1 (MQ=255)
         ttAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCgg                                                                                                                                         <  2:8050445/150‑1 (MQ=255)
           aGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGt                                                                                                                                       >  2:7723903/1‑150 (MQ=255)
             cTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTaa                                                                                                                                     <  2:6344810/150‑1 (MQ=255)
             cTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTaa                                                                                                                                     >  2:9026828/1‑150 (MQ=255)
              ttCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTaaa                                                                                                                                    <  1:5744917/150‑1 (MQ=255)
               tCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAAt                                                                                                                                   <  1:893581/150‑1 (MQ=255)
                   cgcgcCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGAc                                                                                                                               <  2:2293941/150‑1 (MQ=255)
                   cgcgcCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGAc                                                                                                                               <  2:8521874/150‑1 (MQ=255)
                    gcgcCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACa                                                                                                                              <  1:4824679/150‑1 (MQ=255)
                       ccGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTa                                                                                                                           <  1:3879687/150‑1 (MQ=255)
                        cGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTaa                                                                                                                          >  1:542419/1‑150 (MQ=255)
                        cGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTaa                                                                                                                          >  1:7820174/1‑150 (MQ=255)
                         ggAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAAt                                                                                                                         <  2:6577684/150‑1 (MQ=255)
                         ggAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAAt                                                                                                                         <  1:2564593/150‑1 (MQ=255)
                         ggAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAAt                                                                                                                         <  2:50517/150‑1 (MQ=255)
                               ccTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACt                                                                                                                   >  1:3526751/1‑150 (MQ=255)
                                 tGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGt                                                                                                                 >  1:9627388/1‑150 (MQ=255)
                                 tGCCAAATCCGGTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGt                                                                                                                 <  2:8972067/150‑1 (MQ=255)
                                     aaaTCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGc                                                                                                             <  2:9684387/150‑1 (MQ=255)
                                         ccGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGt                                                                                                         <  2:6217327/150‑1 (MQ=255)
                                           gTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGa                                                                                                       >  1:8486231/1‑150 (MQ=255)
                                           gTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGa                                                                                                       >  1:10017621/1‑150 (MQ=255)
                                                 gAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTgc                                                                                                 <  1:9925758/150‑1 (MQ=255)
                                                  aaGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTgcg                                                                                                >  1:7298719/1‑150 (MQ=255)
                                                    gATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGcc                                                                                              >  2:1760791/1‑150 (MQ=255)
                                                     aTGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCa                                                                                             <  2:4179088/150‑1 (MQ=255)
                                                     aTGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTAGTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCa                                                                                             <  2:3325906/150‑1 (MQ=255)
                                                        gAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTc                                                                                          >  2:8914815/1‑150 (MQ=255)
                                                         aaaaaGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTa                                                                                                               >  1:2048539/1‑128 (MQ=255)
                                                          aaaaGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAg                                                                                        >  2:6930297/1‑150 (MQ=255)
                                                           aaaGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGc                                                                                       <  2:2507997/150‑1 (MQ=255)
                                                              gTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGt                                                                                    <  1:8743250/150‑1 (MQ=255)
                                                               tCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTg                                                                                   >  1:4355876/1‑150 (MQ=255)
                                                               tCAGCGCGGGCTATGGCGATCGCAGTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTg                                                                                   >  1:1816588/1‑150 (MQ=255)
                                                                   cgcgGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAAt                                                                               >  2:7905412/1‑150 (MQ=255)
                                                                    gcgGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAAtt                                                                              <  2:1313730/150‑1 (MQ=255)
                                                                    gcgGGCTATGGCGATCGCATTAGTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAAtt                                                                              <  1:1485581/150‑1 (MQ=255)
                                                                     cgGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTg                                                                             >  2:531067/1‑150 (MQ=255)
                                                                     cgGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTg                                                                             >  1:671308/1‑150 (MQ=255)
                                                                     cgGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTg                                                                             <  1:6377930/150‑1 (MQ=255)
                                                                        gCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTc                                                                          <  2:3882741/150‑1 (MQ=255)
                                                                         cTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCt                                                                         >  2:5242813/1‑150 (MQ=255)
                                                                         cTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCt                                                                         <  1:4539942/150‑1 (MQ=255)
                                                                         cTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTATGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCTAACTGTTAGCCGGTGAAATTGCGCCAGTCAGCGGTGAAATTGGTCt                                                                         <  1:2189775/150‑1 (MQ=255)
                                                                          tATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTg                                                                        >  1:6275274/1‑150 (MQ=255)
                                                                           aTGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTgg                                                                       <  2:9492989/150‑1 (MQ=255)
                                                                            tGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGc                                                                      >  1:4082593/1‑150 (MQ=255)
                                                                            tGGCGAACGCATAATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAAAAAAACTGTTAGCCGGTGAACTTCCGCCAGTCAGCGGTGAAATAGGTCTGGc                                                                      >  2:7531092/1‑150 (MQ=255)
                                                                                gATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGaaa                                                                  <  1:9203757/150‑1 (MQ=255)
                                                                                gATCGAATTATTCTCGACTCTATTTAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCTGTGAACTTGCGCCAGTCAGCGGTGTAATTGGTCTGGCGaaa                                                                  <  1:4914377/150‑1 (MQ=255)
                                                                                 aTCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAg                                                                 <  2:4336696/150‑1 (MQ=255)
                                                                                 aTCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAg                                                                 <  1:1161880/150‑1 (MQ=255)
                                                                                     cATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGAt                                                             >  2:1626118/1‑150 (MQ=255)
                                                                                     cATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGAt                                                             <  1:8228004/150‑1 (MQ=255)
                                                                                     cATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGAt                                                             >  1:8853160/1‑150 (MQ=255)
                                                                                      attattCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACACTGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATc                                                            <  2:8288190/150‑1 (MQ=255)
                                                                                       ttattCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCa                                                           <  2:4717648/150‑1 (MQ=255)
                                                                                         attCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCaaa                                                         <  2:192330/150‑1 (MQ=255)
                                                                                           tctcGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACt                                                       <  2:2675624/150‑1 (MQ=255)
                                                                                           tctcGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACt                                                       <  2:2814935/150‑1 (MQ=255)
                                                                                             tcgactcgaTTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGTTCAAACTCg                                                     >  1:2016450/1‑150 (MQ=255)
                                                                                             tcgactcgaTTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCg                                                     >  1:7097344/1‑150 (MQ=255)
                                                                                             tcgactcgaTTAAACTGAACCTGGTGCCCGGCTCGCGTATAGGTCAGTTAGGCCACAATGGCGCGGGTAAAACGACATTAATCAAACTGTTAGCCGGTGAACTTCCGCCAGTCAGCACTGAAATAGGTCTGGCGAAAGGGATCAAACTCg                                                     >  2:7175593/1‑150 (MQ=255)
                                                                                             tcgactcgaTTAAACTGAACCTGGTGCCCGGCTAGCGTATTGGTCTGTAAGGCCACAATGGCGCTGGTAAATCGACATTAATCAAACTGATAGCCGGTGAACTTGAGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCg                                                     >  1:5242252/1‑150 (MQ=255)
                                                                                              cgactcgaTTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCgg                                                    <  2:2029063/150‑1 (MQ=255)
                                                                                              cgactcgaTTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCgg                                                    <  1:3329172/150‑1 (MQ=255)
                                                                                               gactcgaTTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGc                                                   <  1:7536987/150‑1 (MQ=255)
                                                                                                  tcgaTTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTAc                                                >  1:2734714/1‑150 (MQ=255)
                                                                                                        aaaCTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGcc                                          <  1:9447011/150‑1 (MQ=255)
                                                                                                         aaCTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGccc                                         >  2:5864000/1‑150 (MQ=255)
                                                                                                          aCTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCa                                        <  2:2122188/150‑1 (MQ=255)
                                                                                                          aCTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCa                                        >  1:9766589/1‑150 (MQ=255)
                                                                                                               aCCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTACATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCCTc                                   >  2:3474766/1‑150 (MQ=255)
                                                                                                               aCCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATc                                   <  1:8673709/150‑1 (MQ=255)
                                                                                                               aCCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATc                                   >  2:6873470/1‑150 (MQ=255)
                                                                                                                    gTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACtt                              <  1:7425484/150‑1 (MQ=255)
                                                                                                                       cccGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGaa                           <  2:9871863/150‑1 (MQ=255)
                                                                                                                          ggCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATAc                        <  1:2385320/150‑1 (MQ=255)
                                                                                                                           gCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATAcc                       >  2:4910308/1‑150 (MQ=255)
                                                                                                                            cTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCt                      <  2:3542920/150‑1 (MQ=255)
                                                                                                                             tCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTg                     >  2:8690722/1‑150 (MQ=255)
                                                                                                                               gcgTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTgcg                   >  2:5465506/1‑150 (MQ=255)
                                                                                                                                 gTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTgcgcg                 >  2:4462256/1‑150 (MQ=255)
                                                                                                                                  tATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTgcgcgc                <  1:6883113/150‑1 (MQ=255)
                                                                                                                                       gTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCcgacg           >  1:6589521/1‑150 (MQ=255)
                                                                                                                                        tCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCcgacga          >  1:4661102/1‑150 (MQ=255)
                                                                                                                                         cTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGaa         >  1:6123082/1‑150 (MQ=255)
                                                                                                                                          tGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCcgacgact        >  1:7749335/1‑148 (MQ=255)
                                                                                                                                          tGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAAt        >  1:8085638/1‑150 (MQ=255)
                                                                                                                                          tGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGGGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAAt        >  1:5945949/1‑150 (MQ=255)
                                                                                                                                           gTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAATc       >  2:5755260/1‑150 (MQ=255)
                                                                                                                                            ttAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGTGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAATCa      <  2:4555310/150‑1 (MQ=255)
                                                                                                                                            ttAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAATCa      >  2:7297764/1‑150 (MQ=255)
                                                                                                                                              aGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAATCAcc    <  2:8379305/150‑1 (MQ=255)
                                                                                                                                                gCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAATCACCTa  >  1:3145928/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGTTCCGCTTTAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCGCAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAATCACCTA  >  NC_000913/3482178‑3482471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A