Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F52 I0 R1 112 248.4 7913808 99.1% 7842583 149.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,815,809 Δ1 bp 27.3% intergenic (‑41/+25) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,815,8090G.27.3% 455.5 / 271.3 224intergenic (‑41/+25)pyrE/rphorotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
Reads supporting (aligned to +/- strand):  ref base G (80/61);  new base . (55/6);  total (141/83)
Fisher's exact test for biased strand distribution p-value = 1.51e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

Only 100 of 231 total aligned reads displayed.

GTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAA  >  NC_000913/3815661‑3815957
                                                                                                                                                     |                                                                                                                                                    
   gaagaaATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑‑AAAAAAAGGCGAc                                                                                                                                                 >  1:2611268/1‑150 (MQ=255)
       aaaTAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACtca                                                                                                                                              >  1:1521804/1‑150 (MQ=255)
          tAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACtcatca                                                                                                                                           >  1:3393244/1‑150 (MQ=255)
             gggCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAAGGCGACTCATCAGt                                                                                                                                         >  1:2087218/1‑150 (MQ=255)
             gggCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAA‑GCGACTCATCAGTCg                                                                                                                                       >  2:2747227/1‑150 (MQ=255)
              ggCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAA‑GCGACTCATCAGTCGc                                                                                                                                      >  2:3182146/1‑150 (MQ=255)
                         cgggATTTCAGCTTAAACGCGACAAAATTTAACCCCTGCTTGCTAAGCGAAAATACAATAAACTGGCGCTTTTTTGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCAACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATc                                                                                                                            <  1:1808090/148‑1 (MQ=255)
                         ccGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTCCAGAC‑AAAAAAAA‑GCGACTCATCAGTCGCCTTGAAAATCa                                                                                                                           >  1:3211971/1‑150 (MQ=255)
                         ccGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCa                                                                                                                           >  2:3433737/1‑150 (MQ=255)
                          cGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAg                                                                                                                          >  1:3471531/1‑150 (MQ=255)
                             ttttCAGCTTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGAAATTTCAATAAACTGGCGGTTATTTGTTTTCTTGCCTTCGCTCCTCATCTTACTTTTCTCCAGA‑‑AAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGttt                                                                                                                       <  2:606143/149‑1 (MQ=255)
                             ttttAAGCTTAAACTCGCAAAAATTTAACACCTGCTTGCTAAGCGAAAATTCAATAACGTGGCGCTGATATGGTTTATTGCCTTGGCTCCTAATCTTACTTTTCTACAGAC‑AAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGttt                                                                                                                       <  2:1289014/149‑1 (MQ=255)
                             aTTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGtt                                                                                                                        <  2:2064707/150‑1 (MQ=255)
                               ttCAGCGTAACCTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑‑AAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGc                                                                                                                     <  1:1418650/150‑1 (MQ=255)
                               ttCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑‑AAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGc                                                                                                                     >  2:3516046/1‑150 (MQ=255)
                                tCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGc                                                                                                                     <  2:2673189/150‑1 (MQ=255)
                                tCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGAAAATTCAATAAACTGGCGCTGTTATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGc                                                                                                                     <  1:3670226/150‑1 (MQ=255)
                                 cAGCTTAAACTCCCCAAAAATTAACACCTGCTTGCTAAGCGCAAATACAATAAACTGGCGCTCATATGGTTTCATGCCTTCGCTCCACAAATTACTTTACTACACAC‑AAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGcc                                                                                                                    <  1:1960738/150‑1 (MQ=255)
                                   gCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAgc                                                                                                                 >  2:3786679/1‑150 (MQ=255)
                                    cGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAgcg                                                                                                                >  2:1503931/1‑150 (MQ=255)
                                       aaaCTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATTCCTTCGATACTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCC                                                                                                              <  2:2906158‑M1/150‑1 (MQ=255)
                                       aaaCTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCC                                                                                                              >  1:1059495‑M1/1‑150 (MQ=255)
                                           tCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCT                                                                                                          >  2:1186289‑M1/1‑150 (MQ=255)
                                           tCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCT                                                                                                          >  2:3942348‑M1/1‑150 (MQ=255)
                                            cGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTGCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTT                                                                                                         <  1:1118788‑M1/150‑1 (MQ=255)
                                             gCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCT                                                                                                       >  1:939442‑M1/1‑150 (MQ=255)
                                             gCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑‑AAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCT                                                                                                       >  1:3741385‑M1/1‑150 (MQ=255)
                                              ccAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTG                                                                                                      >  1:1691067‑M1/1‑150 (MQ=255)
                                               cAAACTTTAACATCTGCTTTCTAAGCTAATATTCAATAATCGGGCTGTGATTTGTTTTTATGATTTCGCTACTCATCTTAATTTTCTACAGAC‑TAAATAAAGGCGACTCATCAGTCGCGTTAAAAATCATTTTGCCAGCGCCGCCTTCTg                                                                                                      <  1:3569304/150‑1 (MQ=255)
                                               cAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTG                                                                                                      >  1:2830678‑M1/1‑150 (MQ=255)
                                               cAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGC                                                                                                     >  1:773441‑M1/1‑150 (MQ=255)
                                                aaaCTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTG                                                                                                      >  2:1217020‑M1/1‑150 (MQ=255)
                                                aaaCTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCg                                                                                                    >  1:757423/1‑150 (MQ=255)
                                                aaaCTTTAACACCTGCTTGCTAAGCGCAAAATCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAAGGCGACTCATCAGGCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTG                                                                                                      >  2:2086280‑M1/1‑150 (MQ=255)
                                                 aaCTTTAACACCTGCTTGCTAAGCGAAAATGCATCAAACGGGGGATTATTTGGTTCCTGGCCTTCGCTCCACATATTACTTTTACCCACA‑‑AAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGt                                                                                                   <  2:1066557/150‑1 (MQ=255)
                                                    tttAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAA‑GCGACTCATCAGTCGCCGTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGc                                                                                                >  2:2487867/1‑150 (MQ=255)
                                                    tttAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑‑AAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGc                                                                                                >  2:2288296/1‑150 (MQ=255)
                                                    tttAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑‑AAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGc                                                                                                <  2:904984/150‑1 (MQ=255)
                                                    tttAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATACGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑‑AAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcc                                                                                                <  1:1639471‑M1/150‑6 (MQ=255)
                                                        acacCTGCTTGATAAGCGCAAATACAATAAAATGGCGCTGATATGGTTTCATGAATTCGCTCCTCATCTAACTTTTCTACAGAC‑AAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATAAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACa                                                                                            >  2:1363447/1‑150 (MQ=255)
                                                         cacCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctg                                                                                            >  1:3845822‑M1/1‑141 (MQ=255)
                                                         cacCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACaa                                                                                           >  1:843828/1‑150 (MQ=255)
                                                          acCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAAt                                                                                          >  1:3916730/1‑150 (MQ=255)
                                                                  gctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaat                                                                                   >  1:1462115‑M1/1‑132 (MQ=255)
                                                                  gctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtccccggcacttcaat                                                                                   >  2:3878940‑M1/1‑132 (MQ=255)
                                                                  gctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcga                                                                                  >  1:379460/1‑150 (MQ=255)
                                                                    tAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatga                                                                                 >  2:2257821‑M1/1‑130 (MQ=255)
                                                                    tAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatga                                                                                 <  2:3357234‑M1/150‑21 (MQ=255)
                                                                     aaGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgagtcgattc                                                                               >  2:3283751/1‑150 (MQ=255)
                                                                     aaGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑‑AAAAAAAGGCGACTCATCAGTCGCCTTAAAACTCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcgattc                                                                               <  2:2962035/150‑1 (MQ=255)
                                                                       gcgcAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTccc                                                                             >  2:83874/1‑150 (MQ=255)
                                                                           aaaTTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgccc                                                                          <  1:1396002‑M1/150‑28 (MQ=255)
                                                                           aaaTTAAATAAACTGGCTCTTATTTGGTTTCTTGCCTTCGCTCCTCATCTTACTTTTCTACACAC‑AAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCg                                                                         <  2:860733/150‑1 (MQ=255)
                                                                            aaTTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCgg                                                                        >  2:2037586/1‑150 (MQ=255)
                                                                                 aaTAAACTGGCGCTGATATGTTTTCATGCATTAGCTCCAAATCTTACTTTTCTACAGAC‑‑AAAAAAAGGCGACTCAGCAGTCGCCTTAAAAAACAGTTTGCCAGCGCCGCCTGCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCag                                                                   >  2:3860346/1‑150 (MQ=255)
                                                                                 aaTAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttc                                                                    >  2:2836380‑M1/1‑117 (MQ=255)
                                                                                  aTAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcg                                                                   >  1:3355409‑M1/1‑116 (MQ=255)
                                                                                          gcgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCcaacaa                                                          >  2:3509077/1‑150 (MQ=255)
                                                                                              ttttttGGTTTCTTGCCTTCGCTCCAAATATTAATTCTC‑AAAAAA‑AAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATg                                                      <  1:2800843/145‑1 (MQ=255)
                                                                                              tGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATg                                                      >  2:2196710/1‑150 (MQ=255)
                                                                                                atatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑‑AAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAg                                                    <  1:116374/150‑1 (MQ=255)
                                                                                                 tatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGt                                                   <  2:3935488/150‑1 (MQ=255)
                                                                                                   tGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcat                                                  <  2:1630748‑M1/150‑52 (MQ=255)
                                                                                                        tCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCtt                                            >  1:823525/1‑150 (MQ=255)
                                                                                                         cATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTc                                           >  1:3605117/1‑150 (MQ=255)
                                                                                                         cATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTc                                           >  1:3749954/1‑150 (MQ=255)
                                                                                                           tGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑‑AAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAt                                         <  2:3671098/150‑1 (MQ=255)
                                                                                                              cTTCGCTCCTAATATTACTTTTATACACA‑‑AAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATggg                                      <  1:2270246/150‑1 (MQ=255)
                                                                                                               ttCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAATGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATggg                                      >  2:2990844/1‑150 (MQ=255)
                                                                                                               ttCGCTCCTCATCTTACTTTTCTACAGAC‑‑AAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGt                                     <  2:728612/150‑1 (MQ=255)
                                                                                                                  gCTCATAATTTTACTTTACCACACA‑‑AAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGaa                                  <  1:2675238/150‑1 (MQ=255)
                                                                                                                     cctcATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcaga                                <  2:2127711‑M1/150‑70 (MQ=255)
                                                                                                                     cctcATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcaga                                <  2:1059495‑M1/150‑70 (MQ=255)
                                                                                                                        cATCTTACTTTTCTACAGAC‑‑AAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTc                            <  2:2611268/150‑1 (MQ=255)
                                                                                                                          tCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCg                           >  1:797110/1‑150 (MQ=255)
                                                                                                                           cTTACTTTTCTACAGAC‑AAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGcc                         >  2:3004669/1‑150 (MQ=255)
                                                                                                                            ttACTTTTCTACAGAC‑AAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCt                        >  1:465478/1‑150 (MQ=255)
                                                                                                                                ttttCTACAGAC‑AAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTg                    >  1:517302/1‑150 (MQ=255)
                                                                                                                                ttttCTACAGAC‑AAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTg                    >  1:3047003/1‑150 (MQ=255)
                                                                                                                                 tttCTACAGAC‑‑AAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGc                   >  1:2400706/1‑150 (MQ=255)
                                                                                                                                   tCTACAGAC‑‑AAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCg                 <  1:2330234/150‑1 (MQ=255)
                                                                                                                                   tCTACAGAC‑‑AAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCg                 <  1:1890599/150‑1 (MQ=255)
                                                                                                                                     tACAGAC‑AAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTc               >  2:529759/1‑150 (MQ=255)
                                                                                                                                        aGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattccaga             >  1:2829558‑M1/1‑62 (MQ=255)
                                                                                                                                         gAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAACATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattccagat            <  2:1446102‑M1/150‑90 (MQ=255)
                                                                                                                                          agaaaaaaaaaCGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTcccc            <  2:1807063/147‑1 (MQ=255)
                                                                                                                                          aC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCt           >  1:3624428/1‑150 (MQ=255)
                                                                                                                                            acaaaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg          <  1:2197519/148‑1 (MQ=255)
                                                                                                                                            acaaaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg          <  1:3671248/148‑1 (MQ=255)
                                                                                                                                             aaaaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattccagatcgc         >  1:247763‑M1/1‑58 (MQ=255)
                                                                                                                                             aaaaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattccagatcgc         >  1:3301261‑M1/1‑58 (MQ=255)
                                                                                                                                               aaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCAc       >  1:2564088/1‑150 (MQ=255)
                                                                                                                                               aaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCAc       >  1:1284494/1‑150 (MQ=255)
                                                                                                                                               aaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCAc       >  2:805194/1‑150 (MQ=255)
                                                                                                                                               aaaaaaAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCAc       >  1:1916758/1‑150 (MQ=255)
                                                                                                                                               aaaaaaAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCAc       >  1:3870289/1‑150 (MQ=255)
                                                                                                                                                aaaaaaGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACt      >  1:1950517/1‑150 (MQ=255)
                                                                                                                                                aaaaaaGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACt      >  1:1544009/1‑150 (MQ=255)
                                                                                                                                                    aGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattccagatcgcaaaccgc  >  1:1073221‑M1/1‑51 (MQ=255)
                                                                                                                                                    aGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCaa  >  1:2315864/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAA  >  NC_000913/3815661‑3815957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A