Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F49 I0 R1	106	531.4	17433176	98.7%	17206544	148.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,504,873	T→A	18.3%	intergenic (+90/+62)	frlR → / ← yhfS	putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,504,873	0	T	A	18.3%	826.3 / 53.4	349	intergenic (+90/+62)	frlR/yhfS	putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand): ref base T (66/199); new base A (63/3); total (146/203)
Fisher's exact test for biased strand distribution p-value = 1.78e-27
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 384 total aligned reads displayed.

CTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCT  >  NC_000913/3504723‑3505022

 tcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCGAACACTAAGCct                                                                                                                                                       >  1:746346/1‑150 (MQ=39)

    gAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCtctc                                                                                                                                                    <  1:1388216/150‑1 (MQ=255)

    gAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCtctc                                                                                                                                                    <  1:2028704/150‑1 (MQ=255)

    gAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCtctc                                                                                                                                                    <  1:2370154/150‑1 (MQ=255)

      aCTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTccc                                                                                                                                                  <  2:6051793/150‑1 (MQ=255)

        tcttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGTATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCGAACCCTCTCCCCa                                                                                                                                                >  2:7102900/1‑150 (MQ=255)

        tcttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAACCGCTccccc                                                                                                                                                >  2:6986393/1‑149 (MQ=255)

         cttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCaa                                                                                                                                               <  2:5340746/150‑1 (MQ=255)

           tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAg                                                                                                                                             <  1:2757085/150‑1 (MQ=255)

           tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGAGAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGGTGACTTGTACATCCCGTACGTTCCCCGCACCCTAACCCGCTCCCCCAAg                                                                                                                                             >  2:4672121/1‑150 (MQ=39)

             tGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCTCACCCAAAACCTCACCCCAAAggg                                                                                                                                           >  2:2064723/1‑150 (MQ=255)

             tGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATGTTACATCCCGTACGGTCCCCGCACCCGAACCCACACCCCCAAggg                                                                                                                                           >  1:7753210/1‑150 (MQ=38)

              gCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAgggg                                                                                                                                          <  1:8284675/150‑1 (MQ=255)

              gCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCCCACCCAAAACCACAACCCAAAgggg                                                                                                                                          >  1:425866/1‑150 (MQ=39)

               ccGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCACACCCAAACCCACACCCCCAACGGGc                                                                                                                                         >  1:4825009/1‑150 (MQ=38)

               ccGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTACCCGCACCCAAAACCTCACCCCAAAGGGGc                                                                                                                                         >  2:8331264/1‑150 (MQ=39)

                 gcgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACCCCCAAAACCACACCCCAAATGGGCgg                                                                                                                                       >  1:226585/1‑149 (MQ=38)

                  cgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAg                                                                                                                                      <  2:1054324/150‑1 (MQ=255)

                  cgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAg                                                                                                                                      <  2:2309974/150‑1 (MQ=255)

                       aTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATTCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCTCTACCCAAATGGGCGCGGGGAc                                                                                                                                 >  2:1185048/1‑150 (MQ=255)

                        tCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGAcc                                                                                                                                >  2:8590670/1‑150 (MQ=255)

                        tCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGAGGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGAcc                                                                                                                                <  1:5803148/150‑1 (MQ=255)

                         cGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCg                                                                                                                               <  2:7614021/150‑1 (MQ=255)

                         cGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGAGAgggttagg                                                                                                                               <  2:5463213/150‑6 (MQ=38)

                           cATCACCTTAACTATTGATAATAAAAGACATTACCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGtt                                                                                                                             <  1:2524711/150‑1 (MQ=255)

                           cATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGtt                                                                                                                             <  1:3202007/150‑1 (MQ=255)

                           cATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCCCACCCGAAACCACACCCCCCAAGGGCGCGGGGGcccgg                                                                                                                             >  2:3968088/1‑147 (MQ=37)

                           cATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCAAACCCACACCCCCAACGGGCGGGGGGGCCggt                                                                                                                             >  1:6607737/1‑148 (MQ=38)

                                  ttAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCGCCCCCAAAACCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACg                                                                                                                      >  1:1041577/1‑150 (MQ=39)

                                  ttAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCGCACCCAAACCCACAACCCAAAGGGGCGAGGGGGCCGGTAAGTACg                                                                                                                      >  1:8369209/1‑150 (MQ=38)

                                         ttGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTa                                                                                                               <  2:7945871/150‑1 (MQ=255)

                                            ataataAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCAAACCCACTACCCAAAGGGGCGAGGGGACCCTTCAGTAAGGAAATTATCt                                                                                                            >  2:4471395/1‑150 (MQ=38)

                                                taaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCGCACCCTAACCCACAACCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGAc                                                                                                        >  2:6851563/1‑150 (MQ=39)

                                                 aaaaGACATTCACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGATGAATTTTACATCCCGTACGTTCCCCTCCCCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACg                                                                                                       <  1:3115787/150‑1 (MQ=255)

                                                 aaaaGACATTAACCGTAGGCAGGATAAGATGCGCCAGCATCGCAACCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACg                                                                                                       <  1:8055113/150‑1 (MQ=255)

                                                  aaaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCCCCCCCAAAACCAAACCCCCAAAGGGCGAGGGGACCCGTCAGTAAGGAAATTATATTGACgg                                                                                                      >  1:6131451/1‑150 (MQ=25)

                                                  aaaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAAACCTCACCCCCAAGGGGCGAGGGGACCCTTCAGTAAGGAATTTAGCATgtagg                                                                                                      >  2:3531395/1‑146 (MQ=38)

                                                   aaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGACTTTTACATCCCGTACGGTGCCCTCACCCGAACCCACTCCCCAAAGGGGCGGGGGGGCCGGTCAGGACGGAAGTTTGCGTGGCggg                                                                                                     >  1:5252809/1‑149 (MQ=37)

                                                   aaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAAACCTCTACCCCCAGGGGCGAGGGGACCGTTCAGTACCGAATGTAGCTTGACGGt                                                                                                     >  2:4113893/1‑150 (MQ=38)

                                                   aaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTAAATCCCGTACGGTGCCCCCACCCCAAACCACACCCCCAAAGGGCGCGGGGGCCCGTCAGTAACGAATTTAACGTGAAggg                                                                                                     >  1:3983984/1‑149 (MQ=19)

                                                    aGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTAGGGAATTTAGCTTGACGGTg                                                                                                    <  2:1829451/150‑1 (MQ=255)

                                                      aCATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCt                                                                                                  <  2:615167/150‑1 (MQ=255)

                                                        aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCGCACCCAAAACCAAAACCCAAAGGGGCGAGGGGACCGGTCAGTAAGGAAATTAGCTTTTCGGTGCTAt                                                                                                >  1:4635020/1‑150 (MQ=37)

                                                        aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACAACCCCAAGGGGCGAGGGGACCGTTAAGTACCGAAATTATCGTGAAGGTGCTAt                                                                                                >  2:2878748/1‑150 (MQ=37)

                                                        aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACACCAAACCACACCCCCAAAGGGCGCGGGGGCCCGTCAGAAACGAAATTAGCATGAACGTGGTAt                                                                                                >  2:8121358/1‑150 (MQ=19)

                                                         ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                                               <  1:8271532/150‑1 (MQ=255)

                                                              cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCa                                                                                          <  2:8415016/150‑1 (MQ=255)

                                                              cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTAAATCCCGTACGGTCCCCACACCCAAAACCCCGACCCCCAGGGGCGCGGGGGCCCGGCAGGACGGGAGTTAGCGTGACGGGGCGAACCaccc                                                                                          >  1:7206662/1‑147 (MQ=16)

                                                                   gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACCGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTaaa                                                                                     <  1:6891804/150‑1 (MQ=255)

                                                                   gCCGGATAAGATGCGCCAGAATCGCATACGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGGCCTAAGCCTAACACTCTCCCCAAAGGGGCGAGGGGACCGTTCATTACGGAATTTTGCTTTTCGGTGCTATGCAGGATTaaa                                                                                     >  2:1442860/1‑150 (MQ=255)

                                                                        atctgATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTg                                                                                <  1:3460203/146‑1 (MQ=255)

                                                                        aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTg                                                                                <  1:5562622/150‑1 (MQ=255)

                                                                        aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTg                                                                                <  2:6295592/150‑1 (MQ=255)

                                                                        aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTg                                                                                <  2:6898252/150‑1 (MQ=255)

                                                                        aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTg                                                                                <  2:3874740/150‑1 (MQ=255)

                                                                          aaGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCAAAACCACTACCCCAAGGGGCGCGGGGACCGTTCAGTACGGAATTTATATTGACGGTTCTATACAGCAATAAAAACAggg                                                                              >  2:4713165/1‑149 (MQ=255)

                                                                            gATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCaa                                                                            <  1:8258795/150‑1 (MQ=255)

                                                                                cgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATAca                                                                        >  2:5564663/1‑149 (MQ=255)

                                                                                  ccAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATActct                                                                      <  1:3749986/150‑1 (MQ=255)

                                                                                     gcatcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCAc                                                                   <  2:3112632/150‑1 (MQ=255)

                                                                                     gcatcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCAc                                                                   <  1:3387796/150‑1 (MQ=255)

                                                                                            atcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAAt                                                            >  2:5095363/1‑150 (MQ=255)

                                                                                              ccGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAACCCACACCCCAAAGGGGCGAGGGGCCCGGTCAGTACGGAAATTAGCGTGACGGTGCTATCCAGCCTTAAATACAGGCAATACTCTCACGCAAAATcc                                                          >  2:4615519/1‑150 (MQ=255)

                                                                                               cGGCGATGCTGGCGCGTTGAATTTTACATCCCGTAAGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGTCCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCg                                                         <  1:5155409/150‑1 (MQ=255)

                                                                                                 gCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgca                                                       <  1:8159477/150‑1 (MQ=255)

                                                                                                 gCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAAACCTCACCCCAAAGGGGCGAGGGGACCCTTCAGTACGGAAATTAGCTTTACGGTGCGAAACAGCAATAAATACAGGCAAAACACTCACCCAAAAAACgca                                                       >  2:983704/1‑150 (MQ=255)

                                                                                                  cGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgcag                                                      <  2:7504061/150‑1 (MQ=255)

                                                                                                           cgcgTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGtctc                                             <  1:750991/150‑1 (MQ=255)

                                                                                                             cgTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCtt                                           <  2:5620597/150‑1 (MQ=255)

                                                                                                               ttGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGTCCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCa                                         <  2:319985/150‑1 (MQ=255)

                                                                                                               ttGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCa                                         <  2:1629500/150‑1 (MQ=255)

                                                                                                               ttGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCa                                         <  2:3707493/150‑1 (MQ=255)

                                                                                                                tGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCAc                                        <  2:688234/150‑1 (MQ=255)

                                                                                                                    ttttACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCt                                    <  1:403956/150‑1 (MQ=255)

                                                                                                                            cccGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCg                            <  2:2729322/150‑1 (MQ=255)

                                                                                                                            cccGTACGTTACCCACACCCTAAAACACAACCCAAAGGGGCGAGGGGACCGTTAAGTACGGAATTTATCATGACGGTGCTATACAGCATTAAATAATGGCAAAAAACACAACCAAAAAACCCAGCACCCTATATTAAACGCATCGATTCg                            >  2:4225657/1‑150 (MQ=255)

                                                                                                                             ccGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCgg                           <  2:2567768/150‑1 (MQ=255)

                                                                                                                             ccGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCgg                           <  1:2089439/150‑1 (MQ=255)

                                                                                                                               gTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGt                         <  2:224612/150‑1 (MQ=255)

                                                                                                                                   gTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAAt                     >  1:6375978/1‑150 (MQ=255)

                                                                                                                                    ttCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATg                    <  2:583031/150‑1 (MQ=255)

                                                                                                                                    ttCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATg                    >  2:4836853/1‑150 (MQ=255)

                                                                                                                                     tCCCCTCACCCTAACCCTCGCCCCAACGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGc                   <  1:289893/150‑1 (MQ=255)

                                                                                                                                       cccTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGa                 <  1:2212326/150‑1 (MQ=255)

                                                                                                                                       cccTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGa                 <  2:6081759/150‑1 (MQ=255)

                                                                                                                                        ccTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGaa                >  1:3682148/1‑150 (MQ=255)

                                                                                                                                          tcaccctcacccTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATc              <  2:3239358/150‑1 (MQ=255)

                                                                                                                                          tCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATc              >  1:5469378/1‑150 (MQ=255)

                                                                                                                                          tCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATc              <  1:11695/150‑1 (MQ=255)

                                                                                                                                              ccTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac          >  1:6701127/1‑150 (MQ=255)

                                                                                                                                                 aaCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAAt       <  1:8590670/150‑1 (MQ=255)

                                                                                                                                                   cccTCGCCCCTTTGGGGAGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGt     >  1:7643041/1‑150 (MQ=255)

                                                                                                                                                     ctctCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTc   >  1:7021519/1‑150 (MQ=255)

                                                                                                                                                     ctctCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTc   >  1:4897290/1‑150 (MQ=255)

                                                                                                                                                     ctctCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTc   >  1:2734710/1‑150 (MQ=255)

                                                                                                                                                     ctctCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTc   >  2:5989230/1‑150 (MQ=255)

                                                                                                                                                     ctctCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTc   >  2:8684572/1‑150 (MQ=255)

                                                                                                                                                      tctcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCt  >  2:6749037/1‑150 (MQ=255)

                                                                                                                                                      tctcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCt  >  2:5687283/1‑150 (MQ=255)

                                                                                                                                                      tctcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCt  >  2:5216586/1‑150 (MQ=255)

CTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCT  >  NC_000913/3504723‑3505022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A