Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A0 F0 I1 R1	29	281.6	8141248	99.5%	8100541	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	2,480,646	2 bp→AT	intergenic (+21/+103)	zwf → / ← gndA	glucose‑6‑phosphate 1‑dehydrogenase (NADP‑dependent)/NADP+‑dependent 6‑P‑gluconate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	2,480,646	0	T	A	98.4%	659.9 / ‑3.7	183	intergenic (+21/+104)	zwf/gndA	glucose‑6‑phosphate 1‑dehydrogenase (NADP‑dependent)/NADP+‑dependent 6‑P‑gluconate dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (0/3); new base A (72/107); total (72/111)
Fisher's exact test for biased strand distribution p-value = 2.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	BS168_reference_genome	2,480,647	0	A	T	99.5%	697.2 / NA	183	intergenic (+22/+103)	zwf/gndA	glucose‑6‑phosphate 1‑dehydrogenase (NADP‑dependent)/NADP+‑dependent 6‑P‑gluconate dehydrogenase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (71/110); minor base G (1/0); total (72/111)
Fisher's exact test for biased strand distribution p-value = 3.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 183 total aligned reads displayed.

CTCTATTTCTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAATAGTTTACTAAGCATTTTCGGTCTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTA  >  BS168_reference_genome/2480502‑2480792

||

 tctATTTCTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATTTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTa                                                                                                                                              <  1:3543885/150‑1 (MQ=255)

 tctATTTCTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTa                                                                                                                                              >  2:1772876/1‑150 (MQ=255)

   tATTTCTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAca                                                                                                                                            <  2:1823159/150‑2 (MQ=255)

    aTTTCTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAcaa                                                                                                                                           <  2:2325481/150‑3 (MQ=255)

    aTTTCTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAcaa                                                                                                                                           <  2:2123740/150‑3 (MQ=255)

    aTTCCTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAcaa                                                                                                                                           <  2:3048198/150‑3 (MQ=255)

        cTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCAt                                                                                                                                      <  1:1156994/150‑1 (MQ=255)

           aaaCATGGGCAGCAAACAAAACGTTATCTCCTAACTAGGAATCAGGCCCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCAtttt                                                                                                                                   <  1:324347/150‑1 (MQ=255)

           aaaCATGGGCAGCAAACAAAACCTTATCTCCTAACTCCGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCAtttt                                                                                                                                   <  2:3783713/150‑1 (MQ=255)

           aaaCATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCAtttt                                                                                                                                   <  1:2300894/150‑1 (MQ=255)

            aaCATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTc                                                                                                                                  <  2:2719109/150‑1 (MQ=255)

                 gggCAGCAAACAAAACCTTATCTCCTAACTACAAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCggctt                                                                                                                             <  1:3095711/150‑4 (MQ=255)

                  ggCAGCAACCACAACCTTACCTCCTAACTACGAATCAGGCTCATTGGGACCGAAAGAATCTGTTGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCggcttt                                                                                                                            <  2:3009355/150‑5 (MQ=255)

                   gcagcaAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGTTGATCGTTTGGTGAAAGCCGGCTTACACTGGTGGAACATATAATAAGAAG‑AAAAATCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑Cttttt                                                                                                                         <  1:3670035/150‑1 (MQ=255)

                   gcagcaAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGAC‑GAAAATGTTTAC‑AAGCATTTTCGG‑Ctttt                                                                                                                          >  1:1077107/1‑150 (MQ=255)

                    cagcaAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑Cttttt                                                                                                                         <  1:1876478/150‑1 (MQ=255)

                    cagcaAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑Cttttt                                                                                                                         <  2:3249264/150‑1 (MQ=255)

                     agcaAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAGAAAAGCC‑GAAAATGTTTAC‑AACCATTTTCggctatttt                                                                                                                         >  2:3696323/1‑143 (MQ=255)

                         aacaaaACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGc                                                                                                                    <  1:3610458/150‑1 (MQ=255)

                          accaaaCCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCt                                                                                                                   <  1:1282536/147‑1 (MQ=255)

                             aaaCCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTTCGCTGaa                                                                                                                >  2:3068904/1‑150 (MQ=255)

                              aaCCTTACCTCCTAACTCCGAATCAGGCTCAATGGGACCGGAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGATTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAAc                                                                                                               <  1:1101941/150‑1 (MQ=255)

                                ccTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaa                                                                                                             >  1:3745070/1‑150 (MQ=255)

                                ccTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATATTCGG‑CTTTATTACGCTGAACaa                                                                                                             >  2:4030186/1‑150 (MQ=255)

                                 cTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaaa                                                                                                            >  2:1063851/1‑150 (MQ=255)

                                     tctcCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGta                                                                                                        >  2:1177324/1‑150 (MQ=255)

                                      ctcCTACCTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGtat                                                                                                       <  2:557039/150‑1 (MQ=255)

                                      ctcCTAACTACGAATCAGGCTCAATGGGACCGAAACAATCTTATGATCTTTTGGTGAAAGATTGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AGACATTTTCGG‑CTTTTTTACGCTGAACAAAGGtat                                                                                                       >  2:2510203/1‑150 (MQ=255)

                                        ccTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATaa                                                                                                     >  2:3981633/1‑150 (MQ=255)

                                        ccTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATaa                                                                                                     <  1:893168/150‑1 (MQ=255)

                                         cTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CCTTTTTACGCTGAACAAAGGTATAAc                                                                                                    >  1:1707365/1‑150 (MQ=255)

                                            aCTACGAATCAGGCACAATGGGACCGAAAGAATTTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAAt                                                                                                 <  2:290021/150‑1 (MQ=255)

                                             caaCGAATCAGGCCCAATGGGACCGAATGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAAtc                                                                                                <  1:2984518/148‑1 (MQ=255)

                                             cTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAAtc                                                                                                <  1:1772876/150‑1 (MQ=255)

                                             cTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAAtc                                                                                                <  2:1034589/150‑1 (MQ=255)

                                                cGACTCAGGCCCGATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTcc                                                                                             <  2:1613357/150‑1 (MQ=255)

                                                cGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTcc                                                                                             <  1:2385488/150‑1 (MQ=255)

                                                 gAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCt                                                                                            >  1:3094519/1‑150 (MQ=255)

                                                   aTCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGGATAACAATCTCCTta                                                                                          >  2:2032542/1‑150 (MQ=255)

                                                   aTCAGGCTCAAGGGGACCGAAAGAATTTGATGATCTTGTGGTGAAAGCCGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTGTTTTACGCTGAACAAAGGTATAACAATCTCCTta                                                                                          <  2:3181406/150‑1 (MQ=255)

                                                       ggCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTtaataa                                                                                      <  1:3172014/150‑1 (MQ=255)

                                                         cTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACa                                                                                    >  2:1227202/1‑150 (MQ=255)

                                                          tCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACaa                                                                                   >  2:2703357/1‑150 (MQ=255)

                                                           cAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGGCGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAGAATGTTTTC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACaaa                                                                                  <  1:523759/150‑1 (MQ=255)

                                                           cAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACaca                                                                                  >  1:2800049/1‑148 (MQ=255)

                                                           cAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACaaa                                                                                  <  1:1520076/150‑1 (MQ=255)

                                                            aaTGGGACCGAAAGAATCTGATGATGTTTCGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAACAAGCCTGAAAT‑GTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACaaaa                                                                                 <  1:3555518/150‑1 (MQ=255)

                                                            aaTGGGACCGAAAGAATCTGATGATCTTTTGGTGTAAGACGGCTTACACTGGTGGAACATATAATAAGAAGTGAAAAGCC‑GAAAATGTTTAC‑AAGCAGTTTCGG‑CGTGTTTTCGCTGAACAAAGGTATAACAATCTCCTTAATAACcaaa                                                                                 >  2:542488/1‑150 (MQ=255)

                                                            aaTGGGACCGAAAGAATCTGATGACCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACaaaa                                                                                 <  2:1169117/150‑1 (MQ=255)

                                                              tGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACaaaa                                                                                 >  2:3693699/1‑148 (MQ=255)

                                                              tGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACaaaa                                                                                 <  1:3693699/148‑1 (MQ=255)

                                                              tGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAAcc                                                                               <  1:1617349/150‑1 (MQ=255)

                                                               gggACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACCAAAccc                                                                              >  2:631933/1‑150 (MQ=255)

                                                               gggACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAAccc                                                                              >  2:1013323/1‑150 (MQ=255)

                                                                 gACCGAAGGTATCTGTTGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAAccccc                                                                            <  2:342379/150‑1 (MQ=255)

                                                                 gACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAAccccc                                                                            <  1:976778/150‑1 (MQ=255)

                                                                 gACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAAccccc                                                                            <  1:2700233/150‑1 (MQ=255)

                                                                     gAAGGAACCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAg                                                                        <  2:684486/150‑1 (MQ=255)

                                                                      aaaGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGCAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGc                                                                       >  2:3844324/1‑150 (MQ=255)

                                                                       aaGATTCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCt                                                                      <  1:1229022/150‑1 (MQ=255)

                                                                       aaGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCAATTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCt                                                                      >  2:1370828/1‑150 (MQ=255)

                                                                        aGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCtt                                                                     <  2:246613/150‑1 (MQ=255)

                                                                          gaTCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTaa                                                                   <  1:2090908/149‑1 (MQ=255)

                                                                           aTCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAag                                                                  >  2:3276225/1‑150 (MQ=255)

                                                                              tgatgaTCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGc                                                               >  1:876405/1‑150 (MQ=255)

                                                                              tgatgaTCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGc                                                               >  2:1629258/1‑150 (MQ=255)

                                                                               gatgatCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCt                                                              >  1:3494314/1‑150 (MQ=255)

                                                                                atgatCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCtt                                                             >  2:2847846/1‑150 (MQ=255)

                                                                                 tgacCTTTTGGGGAAAGACGGGTTCCTCTGGTGGAACATATAATAAGAAGAAGAAAGCC‑GAAAATGTATAC‑AAGCATCTTCGG‑CTTTTTTACGCTGAACAAAGGTGTAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTc                                                            <  2:1108437/150‑1 (MQ=255)

                                                                                    tCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCggg                                                         <  2:501227/150‑1 (MQ=255)

                                                                                     tttttGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAGAGCC‑GAAAATGTTT‑CCAAGCATTTTCGG‑CTTTTTTACTCTGAACAAAGGTATAACAATCACATTAATATCAAAACCCCCAAAGCTCAAGAGCTTCgggg                                                        <  1:1369973/149‑1 (MQ=255)

                                                                                      ttttGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCggggg                                                       >  2:3245243/1‑150 (MQ=255)

                                                                                       tttGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGt                                                      <  2:3045863/150‑1 (MQ=255)

                                                                                          ggTGAAAGACGGCTTACACTGGTGGAACAAATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATAGT‑GGGCTTTTTTACTCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAACAGCTTCGGGGGTTTc                                                   >  2:2219227/1‑150 (MQ=255)

                                                                                              aaaGACGGCTTACCCTGGTGAAACATATAATAAGAAGAAAAAAGCC‑GACACTGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACCAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTAtt                                               <  2:3391828/150‑1 (MQ=255)

                                                                                              aaaGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTAtt                                               >  2:2757852/1‑150 (MQ=255)

                                                                                                agccGGCTTACACTGGTGGAACATATAAGAAGAAGAAAAAAGCG‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGa                                             <  1:2551352/147‑1 (MQ=255)

                                                                                                   cGGCTTACACTGGTGGAACATATAATAAGAAGAAACAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTa                                          <  1:1393181/150‑1 (MQ=255)

                                                                                                    ggCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTAc                                         <  1:2547446/150‑1 (MQ=255)

                                                                                                      cctagtcTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCCTTTTCTGT‑TTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACtt                                       <  1:1494132/144‑1 (MQ=255)

                                                                                                       ttACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTAAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTc                                      <  1:417572/150‑1 (MQ=255)

                                                                                                        tACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCa                                     >  2:3516514/1‑150 (MQ=255)

                                                                                                          cacTGGTGGAACATAAAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATc                                   <  1:914121/150‑1 (MQ=255)

                                                                                                            cTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCa                                 <  2:876405/150‑1 (MQ=255)

                                                                                                             tggtggAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTATTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGATTCTATTGATTCCTTCATCCAt                                >  1:3904084/1‑150 (MQ=255)

                                                                                                              ggtggAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                               >  1:1767116/1‑150 (MQ=255)

                                                                                                               gtggAACATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGGTTCTATTGATTACTTCATCCATTc                              >  2:3609157/1‑150 (MQ=255)

                                                                                                                    aCAAATAATAAGAAGAAAAAGGCC‑GAAATTGTTTAC‑AAGCATTTTCTG‑CTTTTTTACGCAGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTAt                         <  1:3854157/150‑1 (MQ=255)

                                                                                                                     cATATAATAAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCAACCATTCAGTATg                        >  2:2100302/1‑150 (MQ=255)

                                                                                                                      ataaAATAGGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTAACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATgg                       <  1:3284206/150‑1 (MQ=255)

                                                                                                                       tataATAAGAAGAAATAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGa                      <  1:77041/150‑1 (MQ=255)

                                                                                                                        ataataAGAAGAAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGCGTTTCTATTGATTACTTCATCCATTCAGTATGGaa                     >  2:1095516/1‑150 (MQ=255)

                                                                                                                            taaGAAGAAAAAAGCC‑GAAATTGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATg                 <  2:1861348/150‑1 (MQ=255)

                                                                                                                              agaagaAAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGcc               >  2:32120/1‑150 (MQ=255)

                                                                                                                               gaagaaAAAAGCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCct              <  2:2344508/150‑1 (MQ=255)

                                                                                                                                     tacaGCC‑GAAAATGATTAC‑AAGCATTTTCGG‑CCAATTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTg        >  2:2609983/4‑150 (MQ=255)

                                                                                                                                         gCC‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAg    >  2:1813211/1‑150 (MQ=255)

                                                                                                                                          cc‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGGTGCCTTCTTTGTCAGt   >  1:2741481/1‑150 (MQ=255)

                                                                                                                                           c‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGGGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTa  >  2:1530648/1‑150 (MQ=255)

                                                                                                                                           c‑GAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTa  >  2:148145/1‑150 (MQ=255)

||

CTCTATTTCTGAAACATGGGCAGCAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCC‑GAAATAGTTTACTAAGCATTTTCGGTCTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTA  >  BS168_reference_genome/2480502‑2480792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A