Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A21 F66 I1 R1 35 99.0 4347662 95.6% 4156364 127.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA BS168_reference_genome 3,315,630 G→A T167I (ACC→ATC)  hom ← homoserine dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome3,315,6300GA98.9% 315.0 / ‑4.1 90T167I (ACC→ATC) homhomoserine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (39/50);  total (40/50)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01

GCGCGCCAATATCGCCATTTTCCGTGCGGCGTCAAGCCCTTCCACGTCAGAAGTCGGATCAGCTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGGTTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTAACGTGCGCAGAATCGGAATCCCGCCGGCAACACTGGCTTCAAAGTAGATATCGC  >  BS168_reference_genome/3315497‑3315759
                                                                                                                                     |                                                                                                                                 
gcgcgcCAATATCGCCATTTTCCGTGCGGCGTCAAGCCCTTCCACGTCAGAAGTCGGATCAGCTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGAtt                                                                                                                                 >  6:210121/1‑136 (MQ=255)
     ccAATCTCGCCATTTTCCGTGCGGCGTCAAGCCCTGCCACGTCAGAAGTCGGATCAGCTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACATCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATaaa                                                                                                                         <  4:46096/139‑1 (MQ=255)
     ccAATATCGCCATTTTCCGTGCGGCGTCAAGCCCTTCCACGTCAGAAGTCGGATCAGCTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATaaa                                                                                                                         <  8:28179/139‑1 (MQ=255)
           tCGCCATGTTCCGTGCGGCGTCAAGCCCTTCCACGTCAGAAGTCGGATCAGCTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTttg                                                                                                                    <  2:524127/138‑1 (MQ=255)
             gCCATTTTCCGTGCGGCGTCAAGCCCTTCCACGTCAGAAGTCGGATCAGCTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTttgtt                                                                                                                  <  4:501572/138‑1 (MQ=255)
                 ttttCCGTGCGGCGTCAAGCCCTTCCACGTCAGAAGTCGGATCAGCTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGcc                                                                                                             <  4:345502/139‑1 (MQ=255)
                  tttCCGTGCGGCGTCAAGCCCTTCCACGTCAGAAGTCGGATCAGCTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCg                                                                                                            >  4:524264/1‑139 (MQ=255)
                        tGCGGCGTCAAGCCCTTCCACGTCAGAAGTCGGATCAGCTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTcac                                                                                                       >  3:459198/1‑138 (MQ=255)
                        tGCGGCGTCAAGCCCTTCCACGTCAGAAGTCGGATCAGCTTCGGCAAAACCGACATCCTGCGCTTCCTAGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTcac                                                                                                       >  8:179080/1‑138 (MQ=255)
                                  aGCCCTTCCACGTCAGAAGTCGGATCAGCTTCGGCAAAACCGAGATCCTGCGCTTCCTTGCGCCCGTCCTCGGATGGGCTTTTCTCTTTGATCATTTTGATTAAGATTAAGTTTGTTGTGCCGTTCACAATTCCcataa                                                                                            >  6:179511/1‑137 (MQ=255)
                                   gCCCTTCCACGTCAGAAGTCGGATCAGCTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCcatcat                                                                                           <  6:284882/139‑1 (MQ=255)
                                       ttCCACGTCAGAAGTCGGATCAGCTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTtgtg                                                                                                               <  6:132242/115‑1 (MQ=255)
                                       ttCCACGTCAGAAGTCGGATCAGCTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTtgtg                                                                                                               >  5:132242/1‑115 (MQ=255)
                                       ttCCACGTCAGAAGTCGGATCAGCTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTTTGACGTTCACAAATCCCATCAttttt                                                                                       >  4:10573/1‑139 (MQ=255)
                                       ttCCACGTCAGAAGTCGGATCAGCTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCAttttt                                                                                       >  8:38793/1‑139 (MQ=255)
                                       ttCCACGTCAGAAGTCGGATCAGCTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCAttttt                                                                                       >  3:205802/1‑139 (MQ=255)
                                       ttCCACGTCAGAAGTCGGATCAGCTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCAttttt                                                                                       >  7:189601/1‑139 (MQ=255)
                                         ccACGTCAGAAGTCGGATCAGCTTCGGCAAAACCGAGCTCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTg                                                                                      >  3:526360/1‑138 (MQ=255)
                                          cACGTCAGAAGTCGGATCAGCTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTa                                                                                    <  7:38793/139‑1 (MQ=255)
                                                 gAAGTCGGATCAGCTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGAt                                                                             <  4:120615/139‑1 (MQ=255)
                                                 gAAGTCGGATCAGCTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGAt                                                                             <  5:253561/139‑1 (MQ=255)
                                                 gAAGTCGGATCAGCTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGAt                                                                             <  3:524264/139‑1 (MQ=255)
                                                 gAAGTCGGATCAGCTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGAt                                                                             >  5:311921/1‑139 (MQ=255)
                                                 gAAGTCGGATCAGCTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGAt                                                                             <  8:503951/139‑1 (MQ=255)
                                                  aaGTCGGATCAGCTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATc                                                                            >  8:156540/1‑139 (MQ=255)
                                                  aaGTCGGATCAGCTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATc                                                                            >  6:73538/1‑139 (MQ=255)
                                                   aGTCGGATCAGCTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCt                                                                           <  6:316603/139‑1 (MQ=255)
                                                         aTCAGCTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATgag                                                                     <  7:138919/139‑1 (MQ=255)
                                                              cTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAAt                                                                                  <  2:442876/121‑1 (MQ=255)
                                                              cTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAAt                                                                                  >  1:442876/1‑121 (MQ=255)
                                                              cTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGcc                                                                <  7:179080/139‑1 (MQ=255)
                                                              cTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGcc                                                                <  5:351915/139‑1 (MQ=255)
                                                              cTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGcc                                                                <  1:222711/139‑1 (MQ=255)
                                                              cTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGcc                                                                <  5:181838/139‑1 (MQ=255)
                                                              cTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGcc                                                                <  1:8087/139‑1 (MQ=255)
                                                              cTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGcc                                                                <  8:324442/139‑1 (MQ=255)
                                                              cTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGcc                                                                <  4:459198/139‑1 (MQ=255)
                                                              cTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGcc                                                                <  2:358336/139‑1 (MQ=255)
                                                              cTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGcc                                                                <  6:523419/139‑1 (MQ=255)
                                                              cTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGcc                                                                <  4:143081/139‑1 (MQ=255)
                                                              cTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGcc                                                                <  7:415042/139‑1 (MQ=255)
                                                              cTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGcc                                                                <  7:359203/139‑1 (MQ=255)
                                                                 cGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGCCTTc                                                             >  2:424960/1‑139 (MQ=255)
                                                                   gCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGcc                                                                                                             <  2:330946/89‑1 (MQ=255)
                                                                   gCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGcc                                                                                                             >  1:330946/1‑89 (MQ=255)
                                                                        aCCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTAAc                                                      >  3:357138/1‑139 (MQ=255)
                                                                             gaTCCTGCTCTTCCTTGAGCACTTCCTCGTATTGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTATCGTgcg                                                 <  8:301135/139‑1 (MQ=255)
                                                                             gaTCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTAACGTgcg                                                 <  1:224008/139‑1 (MQ=255)
                                                                             gaTCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTAACGTgcg                                                 <  4:261015/139‑1 (MQ=255)
                                                                              aTCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTAACGTgcg                                                 >  7:81983/1‑138 (MQ=255)
                                                                              aTCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTAACGTgcg                                                 >  7:497511/1‑138 (MQ=255)
                                                                              aTCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCACATCATTTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTAACGTgcg                                                 >  7:16123/1‑138 (MQ=255)
                                                                              aTCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTAGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGCATTCCTCTAACGTgcg                                                 >  5:42815/1‑138 (MQ=255)
                                                                              aTCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGGTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTAACGTgcg                                                 <  8:81983/138‑1 (MQ=255)
                                                                               tCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTAACGTgcgc                                                <  1:424960/138‑1 (MQ=255)
                                                                               tCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTAACGTGCGCa                                               <  4:526360/139‑1 (MQ=255)
                                                                                  tGCGCTTCCTTGAGCACTTCCTCGTTTGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGAt                                                                             >  4:197615/1‑106 (MQ=255)
                                                                                  tGCGCTTCCTTGAGCACTTCCTCGTTTGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGAt                                                                             <  3:197615/106‑1 (MQ=255)
                                                                                   gcgcTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTAACGTGCGCAGAAt                                           >  4:237636/1‑139 (MQ=255)
                                                                                    cgcTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTAACGTGCGCAGAATc                                          >  5:93933/1‑139 (MQ=255)
                                                                                             gAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTAACGTGCGCAGAATCGGAATccc                                  <  6:311921/138‑1 (MQ=255)
                                                                                             gAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTAACGTGCGCAGAATCGGAATccc                                  <  5:73538/138‑1 (MQ=255)
                                                                                              aGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTAACGTGCGCAGAATCGGAATCccgc                                >  1:389190/1‑139 (MQ=255)
                                                                                              aGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTAACGTGCGCAGAATCGGAATCccgc                                <  2:124397/139‑1 (MQ=255)
                                                                                                   ttCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGcc                                                                >  4:290687/1‑102 (MQ=255)
                                                                                                   ttCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGcc                                                                <  3:290687/102‑1 (MQ=255)
                                                                                                   ttCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTAACGTGCGCAGAATCGGAATccc                                  <  8:49242/132‑1 (MQ=255)
                                                                                                   ttCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTAACGTGCGCAGAATCGGAATccc                                  >  7:49242/1‑132 (MQ=255)
                                                                                                   ttCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTAACGTGCGCAGAATCGGAATCCCGCCGGc                            >  5:290095/1‑138 (MQ=255)
                                                                                                   ttCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTAACGTGCGCAGAATCGGAATCCCGCCGGc                            >  2:84268/1‑138 (MQ=255)
                                                                                                   ttCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTAACGTGCGCAGAATCGGAATCCCGCCGGc                            >  6:476526/1‑138 (MQ=255)
                                                                                                   ttCCTCGTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTAACGTGCGCAGAATCGGAATCCCGCCGGCa                           >  2:527908/1‑139 (MQ=255)
                                                                                                         gTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTAACGTGCGCAGAATCGGAATCCCGCCGGCAACACt                      <  6:123643/138‑1 (MQ=255)
                                                                                                         gTATGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTAACGTGCGCAGAATCGGAATCCCGCCGGCAACACt                      <  8:189601/138‑1 (MQ=255)
                                                                                                            tGGGCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTAACGTGCGCAGAATCGGAATCCCGCCGGCAACACTGGCt                  <  8:539421/139‑1 (MQ=255)
                                                                                                             gggCTTTTCTCTTTGATCATTTTGGTTAAGATAAAGTTTGTTGTGACGTTCACTATTCCCATCATTTTTGTAATTAGATATGATGAGATGCCTTCCTATAACTTGCGCAGAATCGGAATCCCGCCGGGAACACTGGcct                 >  2:272392/1‑137 (MQ=255)
                                                                                                              ggCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTAACGTGCGCAGAATCGGAATCCCGCCGGCAACACTGGCtt                 <  4:205802/138‑1 (MQ=255)
                                                                                                               gCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCTTTTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTAACGTGCGCAGAATCGGAATCCCGCCGGCACCACTGGCTTc                >  5:504456/1‑138 (MQ=255)
                                                                                                               gCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTAACGTGCGCAGAATCGGAATCCCGCCGGCAACACTGGCTTc                >  4:312955/1‑138 (MQ=255)
                                                                                                               gCTTTTCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTAACGTGCGCAGAATCGGAATCCCGCCGGCAACACTGGCTTCa               <  3:237636/139‑1 (MQ=255)
                                                                                                                   ttCTCTTTGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTAACGTGCGCAGAATCGGAATCCCGCCGGCAACACTGGCTTCAAAg            >  8:142238/1‑138 (MQ=255)
                                                                                                                        tttGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTAACGTGCGCAGAATCGGAATCCCGCCGGCAACACTGGCTTCAAAGTAg         <  8:497511/136‑1 (MQ=255)
                                                                                                                        tttGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTAACGTGCGCAGAATCGGAATCCCGCCGGCAACACTGGCTTCAAAGTAGat       >  7:196271/1‑138 (MQ=255)
                                                                                                                        tttGATCATTTTGATTAAGATAAAGTTTGTTGTCCCGTTCACAATTCCCCTCATTTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTAACGTGCGCAGAATCGGAATCCCGCCGGCAACACTGGCTTCAAAGTAGa        <  2:359357/137‑1 (MQ=255)
                                                                                                                         ttGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGcc                                                                <  4:208160/80‑1 (MQ=255)
                                                                                                                         ttGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGcc                                                                >  3:208160/1‑80 (MQ=255)
                                                                                                                         ttGATCATTTTGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTATTGTAATTCGATCTGATGAGAGGCCTTCCTCTAACGTGCGCAGAATCGGAATCCCGCCGGCAACACTGGCTTCAAAGTAGatat     >  7:12963/1‑139 (MQ=255)
                                                                                                                             tCATTTTGATTAAGATAAAGGCTGTTGAGCCGTTCACAATTCCCATCAATTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTAACGTGCGCAGAATCGGAATCCCGCCGGCAACACTGGCTTCAAAGTAGATATCGc  <  2:484773/138‑1 (MQ=255)
                                                                                                                                  ttGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTAACGTGCGCAGAATCGGAATCCCGCCGGCAacac                       <  7:524734/112‑1 (MQ=255)
                                                                                                                                  ttGATTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTAACGTGCGCAGAATCGGAATCCCGCCGGCAacac                       >  8:524734/1‑112 (MQ=255)
                                                                                                                                     |                                                                                                                                 
GCGCGCCAATATCGCCATTTTCCGTGCGGCGTCAAGCCCTTCCACGTCAGAAGTCGGATCAGCTTCGGCAAAACCGAGATCCTGCGCTTCCTTGAGCACTTCCTCGTATGGGCTTTTCTCTTTGATCATTTTGGTTAAGATAAAGTTTGTTGTGCCGTTCACAATTCCCATCATTTTTGTAATTCGATCTGATGAGAGGCCTTCCTCTAACGTGCGCAGAATCGGAATCCCGCCGGCAACACTGGCTTCAAAGTAGATATCGC  >  BS168_reference_genome/3315497‑3315759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A