Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F67 I0 R1 253 76.5 2897616 95.8% 2775916 135.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,178,500 C→T 47.5% R1075C (CGT→TGT)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,178,5000CT47.5% 20.4 / 191.1 122R1075C (CGT→TGT) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base C (33/31);  new base T (18/40);  total (51/71)
Fisher's exact test for biased strand distribution p-value = 2.76e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

AAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACC  >  CP009273/4178366‑4178637
                                                                                                                                      |                                                                                                                                         
aaGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCg                                                                                                                                      >  1:778676/1‑140 (MQ=255)
aaGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCg                                                                                                                                      >  1:957931/1‑140 (MQ=255)
aaGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCg                                                                                                                                      >  1:957937/1‑140 (MQ=255)
           tttGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGACAAt                                                                                                                           >  1:846575/1‑140 (MQ=255)
           tttGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAAt                                                                                                                           <  1:563884/140‑1 (MQ=255)
           tttGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAAt                                                                                                                           >  1:847484/1‑140 (MQ=255)
                   cTTTACTGACATGATCGACGGCCAGACCCTTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATg                                                                                                                   <  2:676016/140‑1 (MQ=255)
                   cTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATg                                                                                                                   <  2:1077682/140‑1 (MQ=255)
                   cTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATg                                                                                                                   <  2:1055623/140‑1 (MQ=255)
                    tttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGc                                                                                                                  >  2:52241/1‑140 (MQ=255)
                     ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCt                                                                                                                 <  2:429817/140‑1 (MQ=255)
                     ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCt                                                                                                                 <  1:989724/140‑1 (MQ=255)
                     ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCt                                                                                                                 <  1:378866/140‑1 (MQ=255)
                     ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCt                                                                                                                 <  2:815576/140‑1 (MQ=255)
                       aCTGACATGATCGACGGCCAGACCATGACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGACCGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCa                                                                                                               <  2:324547/140‑1 (MQ=255)
                          gACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAggg                                                                                                            <  2:1077432/140‑1 (MQ=255)
                          gACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAggg                                                                                                            <  1:325126/140‑1 (MQ=255)
                               gATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCTGTCTGTCTTCGCTGGTGGTTATGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGTCACTGAAAATCGTTGATGCTCAGGGTAacg                                                                                                       <  2:90262/140‑1 (MQ=255)
                               gATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacg                                                                                                       <  2:435265/140‑1 (MQ=255)
                               gATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacg                                                                                                       <  1:1279348/140‑1 (MQ=255)
                               gATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacg                                                                                                       <  2:1229939/140‑1 (MQ=255)
                               gATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTGGATGCTCAGGGTAacg                                                                                                       <  2:1149005/140‑1 (MQ=255)
                               gATCGACGGCCAGACCATTACGCGTCAGACCGACGAACGGACCGGTCTGTCTTCGATGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacg                                                                                                       <  2:755389/140‑1 (MQ=255)
                                aTCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGTTCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacga                                                                                                      >  1:636589/1‑140 (MQ=255)
                                aTCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacga                                                                                                      >  1:487027/1‑140 (MQ=255)
                                aTCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacga                                                                                                      >  1:1054486/1‑140 (MQ=255)
                                aTCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacga                                                                                                      >  1:935234/1‑140 (MQ=255)
                                    aCGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGtt                                                                                                  <  1:72298/140‑1 (MQ=255)
                                    aCGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGtt                                                                                                  <  1:568661/140‑1 (MQ=255)
                                     cGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTc                                                                                                 >  2:537244/1‑140 (MQ=255)
                                       gCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTg                                                                                               <  1:7635/140‑1 (MQ=255)
                                       gCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTg                                                                                               >  1:894845/1‑140 (MQ=255)
                                       gCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTg                                                                                               >  1:1056436/1‑140 (MQ=255)
                                       gCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTg                                                                                               >  1:619892/1‑140 (MQ=255)
                                       gCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTg                                                                                               <  1:174294/140‑1 (MQ=255)
                                           gACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATcc                                                                                           >  1:550342/1‑140 (MQ=255)
                                           gACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATcc                                                                                           >  1:1130416/1‑140 (MQ=255)
                                           gACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATcc                                                                                           >  1:1309335/1‑140 (MQ=255)
                                            aCCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATccc                                                                                          >  1:996001/1‑140 (MQ=255)
                                               aTTACGCGTCAGACCGCCGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGACAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAgg                                                                                       <  1:5288/140‑1 (MQ=255)
                                               aTTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAgg                                                                                       <  1:1194803/140‑1 (MQ=255)
                                                ttACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGt                                                                                      <  1:364317/140‑1 (MQ=255)
                                                      gTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAggg                                                                                                            <  2:1316655/112‑1 (MQ=255)
                                                      gTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAggg                                                                                                            >  1:1316655/1‑112 (MQ=255)
                                                        cAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCt                                                                                                                 >  1:248966/1‑105 (MQ=255)
                                                        cAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATGGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGatat                                                                              >  1:881853/1‑140 (MQ=255)
                                                             cgacgaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAg                                                                         <  1:911864/140‑1 (MQ=255)
                                                                cgaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCt                                                                                                                 <  2:326645/97‑1 (MQ=255)
                                                                cgaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCa                                                                                                               >  1:236697/1‑99 (MQ=255)
                                                                cgaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCa                                                                                                               <  2:236697/99‑1 (MQ=255)
                                                                 gaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTTGTAAAGATCTGTGTCCGGCACTTAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCa                                                                     <  1:466552/140‑1 (MQ=255)
                                                                 gaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCa                                                                     <  1:996516/140‑1 (MQ=255)
                                                                 gaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCa                                                                     <  1:907618/140‑1 (MQ=255)
                                                                 gaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCa                                                                     <  1:1198950/140‑1 (MQ=255)
                                                                 gaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCa                                                                     <  1:386976/140‑1 (MQ=255)
                                                                 gaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCa                                                                     <  1:637003/140‑1 (MQ=255)
                                                                        ccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTc                                                              <  2:563917/140‑1 (MQ=255)
                                                                        ccGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTc                                                              <  2:468226/140‑1 (MQ=255)
                                                                           gtctgtctTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTg                                                           <  1:881289/140‑1 (MQ=255)
                                                                           gtctgtctTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTg                                                           <  2:105455/140‑1 (MQ=255)
                                                                                          tggtTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGt                                                                                      <  2:906354/98‑1 (MQ=255)
                                                                                          tggtTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGt                                                                                      <  2:894905/98‑1 (MQ=255)
                                                                                          tggtTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGt                                                                                      >  1:894905/1‑98 (MQ=255)
                                                                                          tggtTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCTCAGTACTTCCTGCCGGGTAAAGCGAtt                                            >  2:523792/1‑140 (MQ=255)
                                                                                             ttgttgtttCCGGAGAACGTTCGGCTGGTGGTTAAGTTCTGTGTCCGGCAATGCAAATCGTTGTTGATCAGGGTAACGAGGTTCTGATCCCAGGTACCGATATGGCAGGTCTGTAATTCATTGCGGGTTATGCGATtttt                                         <  1:697894/133‑1 (MQ=255)
                                                                                                     tCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGa                                 <  2:646731/140‑1 (MQ=255)
                                                                                                     tCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGa                                 <  2:638377/140‑1 (MQ=255)
                                                                                                     tCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGa                                 <  1:709883/140‑1 (MQ=255)
                                                                                                     tCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGa                                 <  2:558240/140‑1 (MQ=255)
                                                                                                     tCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGa                                 <  2:480394/140‑1 (MQ=255)
                                                                                                     tCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGa                                 <  1:491583/140‑1 (MQ=255)
                                                                                                     tCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGa                                 <  2:1138770/140‑1 (MQ=255)
                                                                                                     tCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGa                                 <  2:1198354/140‑1 (MQ=255)
                                                                                                          aGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTggttgttg                            <  2:270970/140‑7 (MQ=255)
                                                                                                          aGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTACAGCTGGAAGATg                            <  2:860619/140‑1 (MQ=255)
                                                                                                          aGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATg                            <  2:1383841/140‑1 (MQ=255)
                                                                                                          aGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATg                            <  1:590113/140‑1 (MQ=255)
                                                                                                          aGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATg                            <  1:827432/140‑1 (MQ=255)
                                                                                                            aaCGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGc                          >  2:873264/1‑140 (MQ=255)
                                                                                                            aaCGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGc                          >  1:998180/1‑140 (MQ=255)
                                                                                                            aaCGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGt                                                     >  1:694468/1‑113 (MQ=255)
                                                                                                            aaCGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACGGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCACCGCAGTACTTCCTGCCGGGTAAAGCGATGGTTCAGCTGGAAGATGGc                          >  2:1359229/1‑140 (MQ=255)
                                                                                                             aCGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGtt                                         >  2:291127/1‑124 (MQ=255)
                                                                                                             aCGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGtt                                         <  1:291127/124‑1 (MQ=255)
                                                                                                               gTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTa                       <  1:505445/140‑1 (MQ=255)
                                                                                                               gTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTa                       <  1:1190030/140‑1 (MQ=255)
                                                                                                                 aCCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGc                                                                            >  2:662372/1‑85 (MQ=255)
                                                                                                                 aCCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGc                                                                            <  1:662372/85‑1 (MQ=255)
                                                                                                                      aGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAggg                                                                                                            <  2:1160469/48‑1 (MQ=255)
                                                                                                                      aGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAggg                                                                                                            >  1:1160469/1‑48 (MQ=255)
                                                                                                                         tggtAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGct             <  1:1318964/140‑1 (MQ=255)
                                                                                                                          tgtAAATATCTGCGTCCGGAACTGAAAATCGTTGATGCTCAGTGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGctc            <  1:28245/139‑1 (MQ=255)
                                                                                                                          ggtAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGctc            <  1:568470/140‑1 (MQ=255)
                                                                                                                             aaaGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTgg         >  1:196043/1‑140 (MQ=255)
                                                                                                                             aaaGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTgg         >  1:515517/1‑140 (MQ=255)
                                                                                                                                  tCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGaca    <  1:1252791/140‑1 (MQ=255)
                                                                                                                                  tCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGaca    >  2:1040134/1‑140 (MQ=255)
                                                                                                                                    tGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACAcc  >  2:741484/1‑140 (MQ=255)
                                                                                                                                    tGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACAcc  >  2:359575/1‑140 (MQ=255)
                                                                                                                                    tGCGTCCGGCACGGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACAcc  >  2:741447/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                         
AAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACC  >  CP009273/4178366‑4178637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A