Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F65 I0 R1 39 317.7 11131448 98.2% 10931081 135.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,178,209 C→T 26.9% R978C (CGT→TGT)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,178,2090CT26.9% 578.8 / 267.6 322R978C (CGT→TGT) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base C (99/133);  new base T (45/41);  total (148/174)
Fisher's exact test for biased strand distribution p-value = 1.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.03e-01

Only 100 of 335 total aligned reads displayed.

ATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGCACAC  >  CP009273/4178071‑4178348
                                                                                                                                          |                                                                                                                                           
aTGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCACGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCg                                                                                                                                            <  2:2097874/140‑1 (MQ=255)
aTGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCg                                                                                                                                            <  2:4295126/140‑1 (MQ=255)
aTGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCg                                                                                                                                            <  1:3226694/140‑1 (MQ=255)
  gCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTa                                                                                                                                          >  2:4719169/1‑140 (MQ=255)
  gCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTa                                                                                                                                          >  2:2067963/1‑140 (MQ=255)
  gCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCATa                                                                                                                                          >  2:3494771/1‑140 (MQ=255)
    gTACGTTCCACATCGGTGGGGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTGATCACTTCCCGTAAt                                                                                                                                        >  2:599539/1‑140 (MQ=255)
     tACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATa                                                                                                                                       <  1:957030/140‑1 (MQ=255)
     tACGTTCCACATCGCTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATa                                                                                                                                       <  2:3271700/140‑1 (MQ=255)
      aCGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATac                                                                                                                                      <  2:1332583/140‑1 (MQ=255)
         ttCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTACACTGGTTATCACTTCCCGTAATactga                                                                                                                                   >  2:3727361/1‑140 (MQ=255)
         ttCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATactga                                                                                                                                   >  1:477050/1‑140 (MQ=255)
         ttCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTACTacgga                                                                                                                                   >  2:4975388/1‑140 (MQ=255)
          tCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATactgaa                                                                                                                                  <  1:5295930/140‑1 (MQ=255)
            cacaTCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATactgaact                                                                                                                                >  1:3557865/1‑140 (MQ=255)
               aTCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATActgaactgaa                                                                                                                             >  2:3891451/1‑140 (MQ=255)
                  ggtggtGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGaactgaaact                                                                                                                          >  2:5278742/1‑140 (MQ=255)
                  ggtggtGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGaactgaaact                                                                                                                          <  1:4711726/140‑1 (MQ=255)
                   gtggtgCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGaactgaaactg                                                                                                                         >  1:3469476/1‑140 (MQ=255)
                    tggtgCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGaactgaaactga                                                                                                                        >  1:5376539/1‑140 (MQ=255)
                    tggtgCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGaactgaaactga                                                                                                                        >  2:2532193/1‑140 (MQ=255)
                      gtgCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGGAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATc                                                                                                                      >  2:5513240/1‑140 (MQ=255)
                         cGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATcgac                                                                                                                   <  1:2238100/140‑1 (MQ=255)
                            cATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGaa                                                                                                                <  1:2723383/140‑1 (MQ=255)
                                 cGTGCGGGTGCTTAATCCAGCTTCAAAGTGAAATAGAAAGGTTGCATCAAGCTCAGCAACTTGAAGTCGGTTGTTAACTCGAGCTGTAAACTGGTTATCACTTCCCGTAATACTTAACTGAAACTGATCGACGAATTCgg                                                                                                           <  2:1119595/140‑1 (MQ=255)
                                 cGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCgg                                                                                                           <  2:1100066/140‑1 (MQ=255)
                                 cGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCgg                                                                                                           <  1:4981761/140‑1 (MQ=255)
                                 cGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCgg                                                                                                           <  2:4107157/140‑1 (MQ=255)
                                 cGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCATAATACTGAACTGAAACTGATCGACGAATTCgg                                                                                                           >  1:4845019/1‑140 (MQ=255)
                                   tGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTACTACTGAACTGAAACTGATCGACGAATTggggc                                                                                                         >  1:5181119/1‑138 (MQ=255)
                                   tGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTc                                                                                                         <  2:1409212/140‑1 (MQ=255)
                                   tGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTc                                                                                                         >  1:4089646/1‑140 (MQ=255)
                                     cGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGt                                                                                                       >  2:3006087/1‑140 (MQ=255)
                                      ggCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGGACTGAAACTGATCGACGAATTCGGTCGTa                                                                                                      >  2:267437/1‑140 (MQ=255)
                                        ctgctgAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACt                                                                                                    >  2:4241796/1‑140 (MQ=255)
                                             gAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTaaaga                                                                                               <  1:3028249/140‑1 (MQ=255)
                                              aaTCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTaaagaa                                                                                              >  1:1807256/1‑140 (MQ=255)
                                              aaTCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTaaagaa                                                                                              >  1:4349056/1‑140 (MQ=255)
                                              aaTCCAGCATACAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCATAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTaaagaa                                                                                              <  2:2972306/140‑1 (MQ=255)
                                                tCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTaaagaaag                                                                                            <  2:1316108/140‑1 (MQ=255)
                                                tCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTaaagaaag                                                                                            <  1:5278742/140‑1 (MQ=255)
                                                tCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTaaagaaag                                                                                            <  2:2311158/140‑1 (MQ=255)
                                                     cATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACa                                                                                       <  1:2382604/140‑1 (MQ=255)
                                                     cATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACa                                                                                       <  1:376871/140‑1 (MQ=255)
                                                     cATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACa                                                                                       <  2:3098878/140‑1 (MQ=255)
                                                     cATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACa                                                                                       >  1:4109568/1‑140 (MQ=255)
                                                     cATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACa                                                                                       <  1:554700/140‑1 (MQ=255)
                                                     cATCCAAGTGAAAAACAAAGGGAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACa                                                                                       <  1:3136706/140‑1 (MQ=255)
                                                       tCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACaaa                                                                                     <  2:2271174/140‑1 (MQ=255)
                                                       tCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACaaa                                                                                     <  1:84055/140‑1 (MQ=255)
                                                       tCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACaaa                                                                                     <  2:1807256/140‑1 (MQ=255)
                                                       tCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACaaa                                                                                     <  2:2391075/140‑1 (MQ=255)
                                                         cAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGt                                                                                   <  1:291542/140‑1 (MQ=255)
                                                               aaaaaCAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTa                                                                             <  1:3863590/140‑1 (MQ=255)
                                                               aaaaaCAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTa                                                                             <  1:2481855/140‑1 (MQ=255)
                                                                 aaaCAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACg                                                                           >  2:1582141/1‑140 (MQ=255)
                                                                 aaaCAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACg                                                                           >  1:2093076/1‑140 (MQ=255)
                                                                    cAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTg                                                                        <  2:5376539/140‑1 (MQ=255)
                                                                    cAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTg                                                                        <  1:3654947/140‑1 (MQ=255)
                                                                    cAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTg                                                                        <  2:167558/140‑1 (MQ=255)
                                                                         gTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAACCTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTa                                                                   <  2:5535408/140‑1 (MQ=255)
                                                                         gTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTa                                                                   <  1:2551882/140‑1 (MQ=255)
                                                                            gCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTg                                                                <  1:1327282/140‑1 (MQ=255)
                                                                             cATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTgg                                                               >  1:5226698/1‑140 (MQ=255)
                                                                               tCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCg                                                             >  1:5491122/1‑140 (MQ=255)
                                                                               tCAAGCTCAGCAACGTCAAGTCGGTTGTGAACTCCAGCGTTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTTATCGACGAATTCTGTCGTACTAAAGAAAGCTACAAAGTACATTACGGTGCGGTACTGGCg                                                             >  1:4744260/1‑140 (MQ=255)
                                                                                   gCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAg                                                         >  2:4428419/1‑140 (MQ=255)
                                                                                    cTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAgg                                                        <  2:3995400/140‑1 (MQ=255)
                                                                                       aGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATCCTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGa                                                     >  2:2340733/1‑140 (MQ=255)
                                                                                       aGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGa                                                     >  2:4843673/1‑140 (MQ=255)
                                                                                       aGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGa                                                     >  2:1941559/1‑140 (MQ=255)
                                                                                            cGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGc                                                 >  2:2648367/1‑139 (MQ=255)
                                                                                            cGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGc                                                 <  1:2648367/139‑1 (MQ=255)
                                                                                            cGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCg                                                >  1:4822360/1‑140 (MQ=255)
                                                                                            cGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCg                                                                      >  2:760505/1‑118 (MQ=255)
                                                                                            cGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCg                                                                      <  1:760505/118‑1 (MQ=255)
                                                                                            cGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTAATCACTTCCTGTAATAGTGAACTGAAACTGATCGACGACTTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCg                                                >  1:3909515/1‑140 (MQ=255)
                                                                                              tGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGaa                                              >  2:1851282/1‑140 (MQ=255)
                                                                                              tGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGaa                                              >  1:5063131/1‑140 (MQ=255)
                                                                                               gAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAAc                                             <  2:5125621/140‑1 (MQ=255)
                                                                                                 aGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTa                                                                             >  1:3114358/1‑106 (MQ=255)
                                                                                                       ttGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCt                                     >  1:2283107/1‑140 (MQ=255)
                                                                                                        tgtgAACTCCAGCGGTAAACTGGTTATCACTTCCTGTACTACTGAACTGAACCTGATCGACGACTTCGGTCGTACTAAAGAAAGCTACAACGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTg                                    <  2:1499204/140‑1 (MQ=255)
                                                                                                                 cAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGaaa                           >  2:1225232/1‑140 (MQ=255)
                                                                                                                      gTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGtt                      >  1:160264/1‑140 (MQ=255)
                                                                                                                       tAAACTGGTTATCACTTCCCGTAAGACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTg                     <  2:3571078/140‑1 (MQ=255)
                                                                                                                         aaCTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCa                   >  1:597844/1‑140 (MQ=255)
                                                                                                                             gggtATCACTTCCTGTAATACTGAACTGAAACTGCTCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACt               <  1:4532947/137‑1 (MQ=255)
                                                                                                                             ggTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACt               <  1:1713890/140‑1 (MQ=255)
                                                                                                                              gTTATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTg              >  2:894877/1‑140 (MQ=255)
                                                                                                                              gTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTg              >  1:4256983/1‑140 (MQ=255)
                                                                                                                              gTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTg              >  1:1522500/1‑140 (MQ=255)
                                                                                                                               ttATCACTTCCTGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTgg             <  1:199128/140‑1 (MQ=255)
                                                                                                                               ttATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTgg             <  1:2771141/140‑1 (MQ=255)
                                                                                                                               ttATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTgg             <  1:1379089/140‑1 (MQ=255)
                                                                                                                               ttATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTgg             <  1:119330/140‑1 (MQ=255)
                                                                                                                               ttATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAAGTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTgg             <  1:4425657/140‑1 (MQ=255)
                                                                                                                               ttATCACTTCCCATAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTgg             <  2:3630508/140‑1 (MQ=255)
                                                                                                                               ttATCACTTCCCATAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTgg             <  2:2838259/140‑1 (MQ=255)
                                                                                                                                        cccGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGcac    >  1:1291017/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                           
ATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGCACAC  >  CP009273/4178071‑4178348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A