Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F65 I0 R1 70 466.8 16344998 98.3% 16067133 135.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,178,501 G→T 37.8% R1075L (CGT→CTT) ‡ rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,178,5010GT37.8% 505.9 / inf 637R1075L (CGT→CTT) ‡rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base G (154/241);  new base T (89/153);  total (243/394)
Fisher's exact test for biased strand distribution p-value = 6.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 642 total aligned reads displayed.

ACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCT  >  CP009273/4178362‑4178639
                                                                                                                                           |                                                                                                                                          
aCCGAAGTAAGCGGTGTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGGTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCg                                                                                                                                            >  2:3007056/1‑140 (MQ=255)
    aaGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCg                                                                                                                                        >  1:1849110/1‑140 (MQ=255)
    aaGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCg                                                                                                                                        >  1:4156891/1‑140 (MQ=255)
     aGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCgg                                                                                                                                       <  2:7573664/140‑1 (MQ=255)
          gCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTg                                                                                                                                  >  1:6992574/1‑140 (MQ=255)
            ggTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGaa                                                                                                                                <  1:1572696/140‑1 (MQ=255)
            ggTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGaa                                                                                                                                <  1:4003537/140‑1 (MQ=255)
               tttGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAAt                                                                                                                             <  1:3172718/140‑1 (MQ=255)
                        tttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGc                                                                                                                    >  1:1372802/1‑140 (MQ=255)
                        tttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGGCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGc                                                                                                                    >  1:2151787/1‑140 (MQ=255)
                         ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGTATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCt                                                                                                                   <  1:6754623/140‑1 (MQ=255)
                         ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGACCGTACCGCAGGTGGTAACGATCTGCGTCCGGCACTGAAAATCGTTGATGCt                                                                                                                   <  1:1707917/140‑1 (MQ=255)
                         ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCt                                                                                                                   <  1:5434982/140‑1 (MQ=255)
                         ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCt                                                                                                                   <  2:1512132/140‑1 (MQ=255)
                         ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCt                                                                                                                   <  1:4933133/140‑1 (MQ=255)
                         ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCt                                                                                                                   <  2:1708200/140‑1 (MQ=255)
                         ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCt                                                                                                                   <  2:7048632/140‑1 (MQ=255)
                         ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCt                                                                                                                   <  2:6417411/140‑1 (MQ=255)
                         ttACTGACAGGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGGGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCt                                                                                                                   <  2:3664568/140‑1 (MQ=255)
                         ttACGGACATGATCGACGGCCAGACCATTACCCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCt                                                                                                                   <  2:2134838/140‑1 (MQ=255)
                          tACTGACATGATCGACGGCCAGACGATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGTGTCTGGATTCCGCAGAACGTACCGCCGGTGGTAAAGATCTGCGTCCGGCACTGAAAATAGTTGATGCTc                                                                                                                  >  1:2415861/1‑140 (MQ=255)
                           aCTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGACAATCGTTGATGCTCa                                                                                                                 >  1:6453638/1‑140 (MQ=255)
                                  tGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGACCGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAac                                                                                                          >  2:4041135/1‑140 (MQ=255)
                                  tGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAac                                                                                                          >  1:7304791/1‑140 (MQ=255)
                                  tGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAac                                                                                                          >  1:8043690/1‑140 (MQ=255)
                                   gATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacg                                                                                                         <  2:7466818/140‑1 (MQ=255)
                                   gATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacg                                                                                                         <  2:6728018/140‑1 (MQ=255)
                                    aTCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacga                                                                                                        >  2:1040150/1‑140 (MQ=255)
                                    aTCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacga                                                                                                        >  1:5761969/1‑140 (MQ=255)
                                    aTCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacga                                                                                                        >  1:3580558/1‑140 (MQ=255)
                                        aCGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGtt                                                                                                    <  2:2816040/140‑1 (MQ=255)
                                              aGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATc                                                                                              <  1:604153/140‑1 (MQ=255)
                                               gACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTg                                                                                                                                  <  2:4147549/103‑1 (MQ=255)
                                               gACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATcc                                                                                             <  1:7779843/140‑1 (MQ=255)
                                                    ttACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGt                                                                                        <  1:2759962/140‑1 (MQ=255)
                                                    ttACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGt                                                                                        <  2:380894/140‑1 (MQ=255)
                                                     tACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTa                                                                                       >  2:4570796/1‑140 (MQ=255)
                                                         cgTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGa                                                                                   <  2:4753480/140‑1 (MQ=255)
                                                          gTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGtt                                                                                                    >  2:667389/1‑122 (MQ=255)
                                                          gTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCt                                                                                                                   <  1:330851/107‑1 (MQ=255)
                                                          gTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGat                                                                                  >  1:4589711/1‑140 (MQ=255)
                                                           tCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGata                                                                                 <  2:5704514/140‑1 (MQ=255)
                                                            cAGACCGACGAACTGACGGGTCGGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGatat                                                                                >  1:1090832/1‑140 (MQ=255)
                                                            cAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGatat                                                                                >  2:3295170/1‑140 (MQ=255)
                                                              gACCGACGAACTGACCGGTCTGTCTTCTCTGGTGGTTGTGGATTCCGCAGAAAGTACCGCAGGTGGTAAAGATCTGCTTCCGTCACTGAAAATCGTTGATGCTCATGGTAACGACGTTCTGATCCCAGGTACCGATATGc                                                                              <  2:7836214/140‑1 (MQ=255)
                                                              gACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGc                                                                              <  2:1188156/140‑1 (MQ=255)
                                                              gACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGc                                                                              <  2:1818190/140‑1 (MQ=255)
                                                              gACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGc                                                                              <  1:1999904/140‑1 (MQ=255)
                                                                     gaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGCTCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCa                                                                       <  2:6269167/140‑1 (MQ=255)
                                                                     gaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCa                                                                       <  1:681886/140‑1 (MQ=255)
                                                                     gaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCa                                                                       <  2:7324417/140‑1 (MQ=255)
                                                                      aaCTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAg                                                                      <  2:1935182/140‑1 (MQ=255)
                                                                                tctgtctTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGc                                                            <  1:7536527/140‑1 (MQ=255)
                                                                                  tgtctTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCg                                                          >  2:6083843/1‑140 (MQ=255)
                                                                                         gCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGc                                                                              >  2:3415784/1‑113 (MQ=255)
                                                                                              tggtTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACAGAAAAACGTTGATGCTCACGGTAACGACGTTCTGATCCCAGGTCCCGATATGCCAGCGCAGTACTACCTGACGGGTCAAACGAtt                                              >  2:5641920/1‑140 (MQ=255)
                                                                                              tggtTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAggg                                                                                                              >  1:4087990/1‑76 (MQ=255)
                                                                                              tggtTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAggg                                                                                                              >  1:2333351/1‑76 (MQ=255)
                                                                                              tggtTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGAtt                                              >  2:2584663/1‑140 (MQ=255)
                                                                                               ggtTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTg                                             <  2:8017299/140‑1 (MQ=255)
                                                                                                    tGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAg                                        <  2:2345297/140‑1 (MQ=255)
                                                                                                         tCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGa                                   <  2:5019649/140‑1 (MQ=255)
                                                                                                         tCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGa                                   <  1:713245/140‑1 (MQ=255)
                                                                                                         tCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGa                                   <  1:2527731/140‑1 (MQ=255)
                                                                                                           cGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGa                                                                                   <  1:835418/90‑1 (MQ=255)
                                                                                                              aGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTCCTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATg                              <  1:7957277/140‑1 (MQ=255)
                                                                                                              aGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATg                              <  1:7057633/140‑1 (MQ=255)
                                                                                                              aGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATg                              <  1:5877476/140‑1 (MQ=255)
                                                                                                              aGAACGTACCGCAGGTGGTAAAGATCTGCGTCCTGCACTGAAAATCGTTGATGCTCAGGTTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATg                              <  1:6664905/140‑1 (MQ=255)
                                                                                                              aGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATg                              <  1:5341377/140‑1 (MQ=255)
                                                                                                                   gTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTa                         <  1:4771179/140‑1 (MQ=255)
                                                                                                                   gTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTa                         <  2:7703785/140‑1 (MQ=255)
                                                                                                                   gTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTa                         <  1:7737636/140‑1 (MQ=255)
                                                                                                                   gTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTa                         <  1:4355090/140‑1 (MQ=255)
                                                                                                                   gTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTa                         <  1:6285498/140‑1 (MQ=255)
                                                                                                                   gTACCGCAGGTGGAAAAGATCTGTGTCCGGCACTGAAAATCGCTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCACTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTa                         <  2:2093171/140‑1 (MQ=255)
                                                                                                                        gCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGAt                    <  2:7135442/140‑1 (MQ=255)
                                                                                                                        gCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATAGTTGATTCTCAGGGTAACGACGTTCTGATGCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGTGTAAAGCGATTGTTCAGCTGGAAGATGGCTTACAGAt                    <  2:4833771/140‑1 (MQ=255)
                                                                                                                        gCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGAt                    <  2:5906867/140‑1 (MQ=255)
                                                                                                                        gCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGAt                    <  2:8096761/140‑1 (MQ=255)
                                                                                                                        gCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGAt                    <  1:5125371/140‑1 (MQ=255)
                                                                                                                          aGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCa                  >  1:553822/1‑140 (MQ=255)
                                                                                                                            gtggtAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGc                >  1:1028212/1‑140 (MQ=255)
                                                                                                                            gtggtAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGc                >  1:2635591/1‑140 (MQ=255)
                                                                                                                             tggtAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGct               <  1:3380584/140‑1 (MQ=255)
                                                                                                                              ggtAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGctc              <  1:3503375/140‑1 (MQ=255)
                                                                                                                              ggtAAAGATCTGCGTCCGGCACTGACAATCGCTGATGCTCAGGGTACCGCCGTTCTGCTCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGctc              <  1:387773/140‑1 (MQ=255)
                                                                                                                               gtAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGa                                                                                   >  1:5253983/1‑70 (MQ=255)
                                                                                                                               gtAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGa                                                                                   <  2:5253983/70‑1 (MQ=255)
                                                                                                                                    gATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGa        <  2:2409358/140‑1 (MQ=255)
                                                                                                                                        tGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGa                                   <  2:5352410/109‑1 (MQ=255)
                                                                                                                                        tGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGa                                   >  1:2956141/1‑109 (MQ=255)
                                                                                                                                        tGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACAcc    >  1:3706583/1‑140 (MQ=255)
                                                                                                                                        tGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGAGTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACAcc    >  1:4245782/1‑140 (MQ=255)
                                                                                                                                          tgTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCt  >  1:6225523/2‑140 (MQ=255)
                                                                                                                                          cGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTACAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCt  >  2:6521654/1‑140 (MQ=255)
                                                                                                                                          cGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCt  <  1:7438123/140‑1 (MQ=255)
                                                                                                                                          cGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCt  >  2:350261/1‑140 (MQ=255)
                                                                                                                                          cGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGAGATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCt  >  2:3866485/1‑140 (MQ=255)
                                                                                                                                          cGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGAGATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCt  <  2:4314453/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
ACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCT  >  CP009273/4178362‑4178639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A