Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F61 I0 R1 274 47.1 1889746 95.1% 1797148 135.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,034,757 C→A 21.3% A13S (GCC→TCC)  mobB ← molybdopterin‑guanine dinucleotide biosynthesis protein B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,034,7570CA21.3% 67.2 / 18.0 38A13S (GCC→TCC) mobBmolybdopterin‑guanine dinucleotide biosynthesis protein B
Reads supporting (aligned to +/- strand):  ref base C (21/8);  new base A (4/4);  total (26/12)
Fisher's exact test for biased strand distribution p-value = 3.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

CATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCAAGCTCCTCTGGCGTATTCACGTTAACAAATGCATCTTTATGATCGCTGAAATCAACCGCATGACCGCCAGCCAGACGCATAAATACCATTAC  >  CP009273/4034619‑4034891
                                                                                                                                          |                                                                                                                                      
cATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCa                                                                                                                                       <  1:166022/140‑1 (MQ=255)
        tCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAg                                                                                                                               >  1:890449/1‑140 (MQ=255)
        tCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGAACAGGCGAg                                                                                                                               >  1:901074/1‑140 (MQ=255)
        tCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGAAAAGGCGAg                                                                                                                               >  1:930155/1‑140 (MQ=255)
        tCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGAAAAGGCGAg                                                                                                                               >  1:696009/1‑140 (MQ=255)
        tCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGAAAAGGCGAg                                                                                                                               >  1:654288/1‑140 (MQ=255)
             gCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACg                                                                                                                          >  1:487512/1‑140 (MQ=255)
                            aTCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTc                                                                                                           >  2:4884/1‑140 (MQ=255)
                             tCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCTTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATAGTTTTTcc                                                                                                          >  1:381711/1‑140 (MQ=255)
                             tCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTcc                                                                                                          >  2:171594/1‑140 (MQ=255)
                             tCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTcc                                                                                                          >  2:117302/1‑140 (MQ=255)
                             tCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTcc                                                                                                          >  1:464998/1‑140 (MQ=255)
                                    cATGGTGCGTATGCTTAATCAGCCCTGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATc                                                                                                   <  1:204123/140‑1 (MQ=255)
                                         tGCGTATGCTTAATCAGCCCTGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTACCGGTATCATCGTTTTTCCTGCCATCGGGCa                                                                                              >  1:549419/1‑140 (MQ=255)
                                             tATGCTTAATCAGCCCTGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCAAGct                                                                                          >  1:421320/1‑140 (MQ=255)
                                                          cccGGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCACGCTCCTCTGGCGTAtt                                                                             >  2:595898/1‑140 (MQ=255)
                                                                 cGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCAAGCTCCTCTGGCGTATTCACGTTa                                                                      <  2:101131/140‑1 (MQ=255)
                                                                 cGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGAAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCAAGCTCCTCTGGCGTATTCACGTTa                                                                      <  2:654288/140‑1 (MQ=255)
                                                                 cGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGAAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCAAGCTCCTCTGGCGTATTCACGTTa                                                                      <  2:696009/140‑1 (MQ=255)
                                                                 cGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGAAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCAAGCTCCTCTGGCGTATTCACGTTa                                                                      <  2:901074/140‑1 (MQ=255)
                                                                 cGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGAAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCAAGCTCCTCTGGCGTATTCACGTTa                                                                      <  2:930155/140‑1 (MQ=255)
                                                                    aTCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCAAGCTCCTCTGGCGTATTCACGTTAACa                                                                   >  1:205532/1‑140 (MQ=255)
                                                                                       ccGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCAAGCTCCTCTGGCGTATTCCCGTTAACAAATGCATCTTTATGATCGc                                                >  2:97972/1‑140 (MQ=255)
                                                                                            aTCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCAAGCTCCTCTGGCGTATTCACGTTAACAAATGCATCTTTATGATCGCTGaaa                                           >  2:903376/1‑140 (MQ=255)
                                                                                               aGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCAAGCTCCTCTGGCGTATTCACGTTAACAAATGCATCTTTATGATCGCTGAAATCa                                        >  2:664138/1‑140 (MQ=255)
                                                                                               aGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCAAGCTCCTCTGGCGTATTCACGTTAACAAATGCATCTTTATGATCGCTGAAACCa                                        >  2:183918/1‑140 (MQ=255)
                                                                                                    tttCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCAAGCTCCTCTGGCGTATTCACGTTAACAAATGCATCTTTATGATCGCTGAAATCAACCGc                                   >  1:622156/1‑140 (MQ=255)
                                                                                                     ttCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCAAGCTCCTCTGGCGTATTCACGTTAACAAATGCATCTTTATGATCGCTGAAATCAACCGCa                                  >  2:274390/1‑140 (MQ=255)
                                                                                                      tCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCAAGCTCCTCTGGCGTATTCACGTTAACAAATGCATCTTTATGATCGCTGAAATCAACCGCAt                                 <  1:171594/140‑1 (MQ=255)
                                                                                                      tCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCAAGCTCCTCTGGCGTATTCACGTTAACAAATGCATCTTTATGATCGCTGAAATCAACCGCAt                                 <  1:117302/140‑1 (MQ=255)
                                                                                                           aGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCAAGCTCCTCTGGCGTATTCACGTTAACAAATGCATCTTTATGATCGCTGAAATCAACCGCATGACCg                            >  2:601173/1‑140 (MQ=255)
                                                                                                                    tttCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCAAGCTCCTCTGGCGTATTCACGTTAACAAATGCATCTTTATGATCGCTGAAATCAACCGCATGACCGCCAGCCAGa                   <  1:408270/140‑1 (MQ=255)
                                                                                                                     ttCCTGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCAAGCTCCTCTGGCGTATTCACGTTAACAAATGCATCTTTATGATCGCTGAAATCAACCGCATGACCGCCAGCCAGAc                  <  1:171749/140‑1 (MQ=255)
                                                                                                                            aCCACTCCACGCGGCAAAGGCGAGTACCGGTATCATCGTTTTTCCTGCCATCGGGCAAGCTCCTCTGGCGTATTCCCGTTAACAAATGCATCTTTATGATCGCTGAAATCAACCCCCTGACCGCCAGCCAGACGCATaaa           <  1:97972/140‑1 (MQ=255)
                                                                                                                                cTCCACGCGGTAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCAAGCTCCTCTGGCGTATTCACGTTAACAAATGCATCTTTATGATCGCTGAAATCAACCGCATGACCGCCAGCCAGACGCATAAATAcc       >  2:326742/1‑140 (MQ=255)
                                                                                                                                cTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCAAGCTCCTCTGGCGTATTCACGTTAACAAATGCATCTTTATGATCGCTGAAATCAACCGCATGACCGCCAGCCAGACGCATAAATAcc       >  2:902737/1‑140 (MQ=255)
                                                                                                                                cTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCAAGCTCCTCTGGCGTATTCACGTTAACAAATGAATCTTTATGATCGCTGAAATCAACCGCATGACCGCCAGCCAGACGCATAAATAcc       >  2:724237/1‑140 (MQ=255)
                                                                                                                                     cgcgGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCAAGCTCCTCTGGCGTATTCACGTTAACAAATGCATCTTTATGATCGCTGAAATCAACCGCATGACCGCCAGCCAGACGCATAAATACCATTAc  >  1:716815/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                      
CATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAAAAGCGTAGTTTTTCCGGTACCACTCCACGCGGCAAAGGCGAGTAACGGTATCATCGTTTTTCCTGCCATCGGGCAAGCTCCTCTGGCGTATTCACGTTAACAAATGCATCTTTATGATCGCTGAAATCAACCGCATGACCGCCAGCCAGACGCATAAATACCATTAC  >  CP009273/4034619‑4034891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A