Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F63 I1 R1	6	130.4	4552742	98.9%	4502661	135.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	4,178,500	C→T	R1075C (CGT→TGT)	rpoC →	RNA polymerase, beta prime subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,178,500	0	C	T	99.4%	611.8 / NA	155	R1075C (CGT→TGT)	rpoC	RNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (55/99); minor base G (0/1); total (55/100)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 155 total aligned reads displayed.

AAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACAC  >  CP009273/4178366‑4178636

aaGTAAGCGGTTTTGTACGCTTTACTGACCTGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCg                                                                                                                                     >  2:641603/1‑140 (MQ=255)

   tAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCa                                                                                                                                  <  2:1812695/140‑1 (MQ=255)

   tAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCa                                                                                                                                  <  2:684972/140‑1 (MQ=255)

     aGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACt                                                                                                                                >  1:1401864/1‑140 (MQ=255)

     aGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACt                                                                                                                                >  2:612622/1‑140 (MQ=255)

     aGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACt                                                                                                                                >  2:1075912/1‑140 (MQ=255)

                aCGCTTTACTGACATGCTCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTg                                                                                                                     <  1:2200194/140‑1 (MQ=255)

                   cTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATg                                                                                                                  <  2:1994674/140‑1 (MQ=255)

                   cTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATg                                                                                                                  <  2:47666/140‑1 (MQ=255)

                   cTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATg                                                                                                                  <  2:419001/140‑1 (MQ=255)

                    tttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGc                                                                                                                 >  1:1444589/1‑140 (MQ=255)

                     ttACTGACATGATCGACGGCCAGCCGATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCGGCAGCACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAACATCGTTGATGCt                                                                                                                <  2:367042/140‑1 (MQ=255)

                     ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCt                                                                                                                <  2:217231/140‑1 (MQ=255)

                     ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCt                                                                                                                <  2:1032955/140‑1 (MQ=255)

                     ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCt                                                                                                                <  2:477988/140‑1 (MQ=255)

                     ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCt                                                                                                                <  2:679120/140‑1 (MQ=255)

                     ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCt                                                                                                                <  2:1260009/140‑1 (MQ=255)

                      tACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCTGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGGTGATGCTc                                                                                                               <  1:473316/140‑1 (MQ=255)

                      tACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTc                                                                                                               >  1:935038/1‑140 (MQ=255)

                               gATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacg                                                                                                      <  1:616242/140‑1 (MQ=255)

                               gATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacg                                                                                                      <  1:2230384/140‑1 (MQ=255)

                               gATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacg                                                                                                      <  1:73268/140‑1 (MQ=255)

                                aTCGACGGCCAGACCATTACGCGTCAGACCGACGACCTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacga                                                                                                     >  1:1916780/1‑140 (MQ=255)

                                aTCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCt                                                                                                                <  1:196561/129‑1 (MQ=255)

                                aTCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCt                                                                                                                >  2:2239191/1‑129 (MQ=255)

                                aTCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacga                                                                                                     >  2:1903474/1‑140 (MQ=255)

                                aTCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacga                                                                                                     >  2:230465/1‑140 (MQ=255)

                                aTCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacga                                                                                                     >  2:1416284/1‑140 (MQ=255)

                                aTCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacga                                                                                                     >  1:1826801/1‑140 (MQ=255)

                                aTCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacga                                                                                                     >  1:1668458/1‑140 (MQ=255)

                                aTCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacga                                                                                                     >  1:1223434/1‑140 (MQ=255)

                                     cGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTc                                                                                                >  1:1867296/1‑140 (MQ=255)

                                     cGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTc                                                                                                >  1:1107594/1‑140 (MQ=255)

                                     cGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTc                                                                                                >  1:716443/1‑140 (MQ=255)

                                     cGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTc                                                                                                >  1:1599599/1‑140 (MQ=255)

                                     cGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTc                                                                                                >  1:553865/1‑140 (MQ=255)

                                          aGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATc                                                                                           >  1:136616/1‑140 (MQ=255)

                                           gACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATcc                                                                                          >  1:145542/1‑140 (MQ=255)

                                           gACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATcc                                                                                          >  1:145844/1‑140 (MQ=255)

                                           gACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATcc                                                                                          <  2:62367/140‑1 (MQ=255)

                                            aCCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATccc                                                                                         <  1:1011623/140‑1 (MQ=255)

                                                ttACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGt                                                                                     <  1:2003037/140‑1 (MQ=255)

                                                ttACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGt                                                                                     <  1:612622/140‑1 (MQ=255)

                                                ttACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGt                                                                                     <  1:1075912/140‑1 (MQ=255)

                                                     cgTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGa                                                                                <  2:1599599/140‑1 (MQ=255)

                                                     cgTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGa                                                                                <  2:1223434/140‑1 (MQ=255)

                                                     cgTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGa                                                                                <  2:1668458/140‑1 (MQ=255)

                                                     cgTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGa                                                                                <  2:805676/140‑1 (MQ=255)

                                                      gTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGat                                                                               >  2:291853/1‑140 (MQ=255)

                                                        cAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGatat                                                                             >  1:1575714/1‑140 (MQ=255)

                                                          gACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGc                                                                           <  2:1162749/140‑1 (MQ=255)

                                                          gACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGc                                                                           <  2:800475/140‑1 (MQ=255)

                                                          gACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGc                                                                           <  1:818389/140‑1 (MQ=255)

                                                          gACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGc                                                                           <  2:412446/140‑1 (MQ=255)

                                                          gACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGc                                                                           <  1:2232049/140‑1 (MQ=255)

                                                          gACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGc                                                                           <  2:2156857/140‑1 (MQ=255)

                                                             cgacgaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAg                                                                        <  2:1495777/140‑1 (MQ=255)

                                                               acgaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAgcg                                                                      <  1:2110200/140‑1 (MQ=255)

                                                               acgaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAgcg                                                                      <  1:1664658/140‑1 (MQ=255)

                                                               acgaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAgcg                                                                      <  1:1732604/140‑1 (MQ=255)

                                                                cgaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAgcgc                                                                     >  1:2271917/1‑140 (MQ=255)

                                                                 gaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCa                                                                    <  2:1401888/140‑1 (MQ=255)

                                                                 gaACTGACCGGTCTGTCTTCGCTGGGGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTGCTGATCCCAGGTACCGATATGCCAGCGCa                                                                    <  1:1018190/140‑1 (MQ=255)

                                                                  aaCTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAg                                                                   <  1:388676/140‑1 (MQ=255)

                                                                      gACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACt                                                               >  1:200024/1‑140 (MQ=255)

                                                                      gACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACt                                                               <  1:2129939/140‑1 (MQ=255)

                                                                           gtctgtctTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTg                                                          <  1:1254034/140‑1 (MQ=255)

                                                                           gtctgtctTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTg                                                          <  1:2166077/140‑1 (MQ=255)

                                                                            tctgtctTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGc                                                         <  1:500066/140‑1 (MQ=255)

                                                                              tgtctTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCg                                                       <  2:145542/140‑1 (MQ=255)

                                                                              tgtctTCGCTGGTGGTTATGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCg                                                       <  2:1066981/140‑1 (MQ=255)

                                                                                          tggtTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGAtt                                           >  2:1526727/1‑140 (MQ=255)

                                                                                             ttCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGtt                                        <  1:1230216/140‑1 (MQ=255)

                                                                                              tCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTc                                       <  2:1886790/140‑1 (MQ=255)

                                                                                                tGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAg                                     <  2:1444589/140‑1 (MQ=255)

                                                                                                tGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAg                                     <  2:46640/140‑1 (MQ=255)

                                                                                                     tCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGa                                <  2:1243589/140‑1 (MQ=255)

                                                                                                     tCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGa                                <  1:1960766/140‑1 (MQ=255)

                                                                                                     tCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGa                                <  2:1375619/140‑1 (MQ=255)

                                                                                                     tCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGa                                <  2:225145/140‑1 (MQ=255)

                                                                                                        gCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGa                             <  2:553865/140‑1 (MQ=255)

                                                                                                        gCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGa                             <  2:1083227/140‑1 (MQ=255)

                                                                                                               gTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTa                      <  2:935038/140‑1 (MQ=255)

                                                                                                               gTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTa                      <  2:1916780/140‑1 (MQ=255)

                                                                                                                 aCCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCt                                                                                                                <  2:138842/48‑1 (MQ=255)

                                                                                                                 aCCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCt                                                                                                                >  1:138842/1‑48 (MQ=255)

                                                                                                                    gCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGAt                 <  2:200024/140‑1 (MQ=255)

                                                                                                                      aGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGa                             <  1:2213914/126‑1 (MQ=255)

                                                                                                                        gtggtAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAACGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGc             >  2:1122029/1‑140 (MQ=255)

                                                                                                                        gtggtAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGc             <  2:2020794/140‑1 (MQ=255)

                                                                                                                        gtggtAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGc             >  2:1574624/1‑140 (MQ=255)

                                                                                                                        gtggtAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGc             >  1:989501/1‑140 (MQ=255)

                                                                                                                        gtggtAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGc             >  1:1908736/1‑140 (MQ=255)

                                                                                                                         tggtAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGct            <  1:149093/140‑1 (MQ=255)

                                                                                                                         tggtAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGct            <  1:230465/140‑1 (MQ=255)

                                                                                                                          ggtAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGctc           <  1:1289349/140‑1 (MQ=255)

                                                                                                                               aGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTg      <  1:2246374/140‑1 (MQ=255)

                                                                                                                                   cTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGc                                                                           >  1:235718/1‑67 (MQ=255)

                                                                                                                                   cTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGc                                                                           <  2:235718/67‑1 (MQ=255)

                                                                                                                                   cTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGGTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGGAACGCGATTGTTCAGCTGGAAGATGGCGTCCAGATCAGCTCTGGTGacac  >  1:510908/1‑140 (MQ=255)

AAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACAC  >  CP009273/4178366‑4178636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A