Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A30 F19 I2 R1
|
13 |
31.9 |
1110306 |
92.5% |
1027033 |
144.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913_3_vch_pgi |
4,343,923 |
G→A |
L184L (CTG→CTA) |
melB → |
melibiose:sodium symporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913_3_vch_pgi | 4,343,923 | 0 | G | A | 96.7%
| 92.7
/ NA
| 30 | L184L (CTG→CTA) | melB | melibiose:sodium symporter |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (13/16); minor base C (1/0); total (14/16) |
| Fisher's exact test for biased strand distribution p-value = 4.67e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGATGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCG‑TCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTAAAGCCATCGTTGCGCTAATTTATAAAAACGA > NC_000913_3_vch_pgi/4343778‑4344063
|
tCGATAAACGTGAGCGCGAACAACTGGTTCCTTTTCCGCGTTATAGAATATGGCTTGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGATGTTCACTCTAGTa < 1:404910/149‑1 (MQ=255)
tAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGATGTTCACTCTAGTACTGa < 2:407732/149‑1 (MQ=255)
caacTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGATGTTCACTCTAGTACTGATCGCCTTTTTTATTGt < 1:387186/149‑1 (MQ=255)
acTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGATGTTCACTCTAGTACTGATCGCCTTTTTTATTGttt < 2:434801/149‑1 (MQ=255)
cTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAGTTATGTCGGCGGTGGCGCTCGGGGATTTGGCTTTCAGATGTTCACTCTCGTACTGATCGCCTTTTTTATTGTTTc > 1:29000/1‑149 (MQ=255)
gTTCCTTCTCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGTAGGTGGCGATCGGGGCTTTGGCTTTTAGATGTTCCCTCTAGTACTGATCGCCTTTTTTATTGTTTCAAc < 2:189680/149‑1 (MQ=255)
tCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGATGTTCACTCTAGTACTGATCGCCTTTTTTATTGTTTCAACca < 1:328708/149‑1 (MQ=255)
tCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGATGTTCACTCTAGTACTGATCGCCTTTTTTATTGTTTCAACCAtcatca > 1:401097/1‑149 (MQ=255)
gCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGATGTTCACTCTAGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAAtgt < 1:507191/149‑1 (MQ=255)
tttGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGATGTTCACTCTAGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTc > 2:184989/1‑149 (MQ=255)
ttACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGATGTTCACTCTAGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCG‑TCa < 1:135333/149‑1 (MQ=255)
gCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGATGTTCACTCTAGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCG‑TCAGACaa > 1:149476/1‑149 (MQ=255)
cAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGATGTTCACTCTAGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCG‑TCAGACAAt > 1:92165/1‑149 (MQ=255)
cAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGCCACTACGGGATTTGGAACTCACCTGTTGACTCCAGTACTGGTCGCCTATTTTTTTTTTTCAGCCATGTTCAGTCTTTGCAAGCTTCATGAAGTCTTTTCG‑TCAGCCAAt > 2:188361/1‑149 (MQ=255)
gCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGATGTTCACGCTAGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCG‑TCAGACAATCAACCGTctg > 1:436235/1‑149 (MQ=255)
tACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGATGTTCACTCTAGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCG‑TCAGACAATCAACCGTctgct < 1:329221/149‑1 (MQ=255)
tACCATTTGATAAGCATGGGTACGGTGGCGATCGTTGATTTGGCCTTCAGATGTTCACTCTAGTACTGAGCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCG‑TCAGACAATCAACCGTctgct > 2:467106/1‑149 (MQ=255)
aCCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGATGTTCACTCTAGTACTGATCGCCTTTTTTTTTTTTTTATCCATCATCACTCTGCGCAATGTGAATGAAGTCTTTTCG‑TCATACAATCAACCGTctactg > 2:514127/1‑149 (MQ=255)
tGTCGGCGGTGGCGATTGGGGATTTGGCTTTCAGATGTTCACTCTAGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCG‑TCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGa < 2:405379/149‑1 (MQ=255)
gggcggTGGGGATCGGGGATTTGGCTTTCAGATGTTCACTCCAGTACTGATCGTCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCG‑TCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGacac < 2:227971/148‑1 (MQ=255)
gcggTGGCGATCGGGGATTTGGCTTTCAGATGTTCACTCTAGTACTGATCTCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCG‑TCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACtt < 2:92165/149‑1 (MQ=255)
gTGCCGATCGGGGATTTAGCTTTCAGATGTTCACTCTAGTTCTTCTCGACTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCG‑TCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTaaa < 1:120923/149‑1 (MQ=255)
gATCGGGGATTTGGCTTTCAGATGTTCACTCTAGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCG‑TCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTAAAGCCAt > 1:299950/1‑149 (MQ=255)
aTTTGGTGTTCAGGTGTTCACTCTAGTACTGATTGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTTCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTAAAGCCATCGTTgcg < 2:376209/149‑1 (MQ=255)
tCAGATGTTCACTCTAGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCAt > 2:427839/1‑70 (MQ=255)
tCAGATGTTCACTCTAGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCAt < 1:427839/70‑1 (MQ=255)
aTGTTCACTCTAGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCG‑TCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTAAAGCCATCGTTGCGCTAATTTATaaaaa < 1:112140/149‑1 (MQ=255)
ttCACTCTAGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCG‑TCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTAAAGCCATCGTTGCGCTAATTTATAAAAACGa > 1:514252/1‑149 (MQ=255)
ttCACTCTAGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCG‑TCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTAAAGCCATCGTTGCGCTAATTTATAAAAACGa > 2:465940/1‑149 (MQ=255)
ttCACTCTAGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCG‑TCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTAAAGCCATCGTTGCACTAATTTATAAAAACGa > 1:394276/1‑149 (MQ=255)
|
TCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCAGGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGATGTTCACTCTGGTACTGATCGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCG‑TCAGACAATCAACCGTCTGCTGAAGGAAGCCATCTGACACTTAAAGCCATCGTTGCGCTAATTTATAAAAACGA > NC_000913_3_vch_pgi/4343778‑4344063
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
