Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F75 I1 R1	21	60.9	2110098	94.8%	2000372	141.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	3,486,397	G→C	C93S (TGT→TCT)	crp →	cAMP‑activated global transcription factor, mediator of catabolite repression

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	3,486,397	0	G	C	100.0%	130.9 / NA	41	C93S (TGT→TCT)	crp	cAMP‑activated global transcription factor, mediator of catabolite repression
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (20/21); total (20/21)

CTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGA  >  NC_000913_3_hsa_tpiA/3486257‑3486540

ctctGTGGCAGTGCTGATCAAAGACGAAGAGGGGAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTCTGAAGTgg                                                                                                                                         <  2:141356/149‑1 (MQ=255)

   tgtgGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCAAAAGCCGCCTCTGAAGTGGCTg                                                                                                                                      >  2:467594/1‑149 (MQ=255)

                aTCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTCTGAAGTGGCTGAAATTTCGTACaa                                                                                                                         >  1:655988/1‑149 (MQ=255)

                    aaGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGCACGTAGCGCATGGGTACGTGCGAAAACCGCCTCTGAAGTGGCTGAAATTGCGTACaacaaa                                                                                                                     >  1:1051180/1‑149 (MQ=255)

                         gAAGAGGGTAAAGAAATGATACTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTCGGCCTGTTTGAAGATCGCCACGTTCATAGATCATGGGTTCGTTCGAAAACCGCCTCTGAAGTGGCTGAAATTTCGTACAAAAAATTTCg                                                                                                                >  2:906675/1‑149 (MQ=255)

                                gTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGACCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTCTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTg                                                                                                         >  2:135502/1‑149 (MQ=255)

                                  aaaGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTCTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGAt                                                                                                       <  1:853200/149‑1 (MQ=255)

                                   aaGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTCTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGAtt                                                                                                      <  1:792699/149‑1 (MQ=255)

                                     gAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCATGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTCTGAAGTGGCTGAAATTTCGTCCAAAAAATTTCCCCACttcattca                                                                                                    >  2:442732/1‑149 (MQ=255)

                                      aaaTGATTCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTCTGAAGTGGCTGAAATTTCGTACAAAACAGGCAGCTAATTGATTCAg                                                                                                   <  2:655988/149‑1 (MQ=255)

                                          gATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTCTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTaa                                                                                               >  1:444608/1‑149 (MQ=255)

                                                ctcCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTCTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCgg                                                                                         <  1:1019962/149‑1 (MQ=255)

                                                      tCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTCTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTc                                                                                   >  1:88391/1‑149 (MQ=255)

                                                       cTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTCTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCt                                                                                  <  1:8719/149‑1 (MQ=255)

                                                            tctgggTGATTTTATTTGCGAACTGGGCGTGTGTGAAGAGGGTCAGGAACGTCGCGCATGGGTACGTGCGAAAACCGCCTCTGAAGTGTCTGAAATTTCGTACAAAAGATTTCGCCCATTGATTCAGGTAAACCCGGACATTCTGATGc                                                                             <  2:122809/146‑1 (MQ=255)

                                                            tCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTCTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGc                                                                             >  1:895566/1‑149 (MQ=255)

                                                            tCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTCTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGc                                                                             >  2:976342/1‑149 (MQ=255)

                                                            tCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTCTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGc                                                                             >  1:894574/1‑149 (MQ=255)

                                                                     ttttATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTCTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTg                                                                    >  1:871899/1‑149 (MQ=255)

                                                                           tGGCGAACTGGGCTGGTTGGAAAAGGGCAAGGAATGTAGCGCATGGGTACGTGCGAAAACCGCCTCTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGa                                                              <  2:256021/149‑1 (MQ=255)

                                                                            agCGAACGTGGCCTGTTGGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTCTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGAt                                                             <  2:1051180/148‑1 (MQ=255)

                                                                                 aCTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTCTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGgcgc                                                        <  2:871899/149‑1 (MQ=255)

                                                                                     ggCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTCTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGcgtcg                                                    >  1:295450/1‑149 (MQ=255)

                                                                                       gcTGTTTGAAGAGAGCCAGGAACGTAGCGCATGGGTACGGGGGAAAACCGCCTCTGAAGTGGCTGAAAAAAAAAAAAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCgtcgtc                                                  <  2:399428/148‑1 (MQ=255)

                                                                                         tGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTCTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTg                                                >  1:481902/1‑149 (MQ=255)

                                                                                          gTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTCTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGc                                               >  2:823396/1‑149 (MQ=255)

                                                                                                   gggCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTCTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACtt                                      <  1:823396/149‑1 (MQ=255)

                                                                                                     gcaaGGAACGTAGCGCATGGGGACGTGCGAAAACCGCCTCTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTcc                                    <  2:186087/146‑2 (MQ=255)

                                                                                                     gCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTCTGAAGTGGCTGAAATTTCGTACAAAAAATTTc                                                                                                                 >  2:146986/1‑72 (MQ=255)

                                                                                                     gCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTCTGAAGTGGCTGAAATTTCGTACAAAAAATTTc                                                                                                                 <  1:146986/72‑1 (MQ=255)

                                                                                                     gCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTCTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCa                                    <  2:894574/149‑1 (MQ=255)

                                                                                                     gCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTCTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCa                                    <  2:895566/149‑1 (MQ=255)

                                                                                                       cAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTCTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTATCTGCACAGATGGCGCGTCGCCTCCAACTCACTTCcga                                  >  2:985442/1‑149 (MQ=255)

                                                                                                               tAGCGCATGGGTACGTGCGAAAACCGCCTCTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGCTGGAACGTCTGCTGCAAGTCACTTCAGAGAAAGTgg                          <  2:496918/149‑1 (MQ=255)

                                                                                                               tAGCGCATGGGGACGTGCAAAAACCGCCTCTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAGAGtcg                          <  2:710484/149‑3 (MQ=255)

                                                                                                                   gcATGGGTACGTGCGAAAACCGCCTCCGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAATGGGCaa                      >  2:821866/1‑149 (MQ=255)

                                                                                                                       gggTACTTGCGAAAACCGCCTCTGAAGTGGCTTACATTTCCGATATAATAGTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTg                  <  1:985442/149‑1 (MQ=255)

                                                                                                                             gTGCGAAAACCGCCTCTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTc            >  1:226744/1‑149 (MQ=255)

                                                                                                                                  aaaaCCGCCTCTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGa       <  1:976342/149‑1 (MQ=255)

                                                                                                                                   aaaCCGCCTCTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGAc      <  2:88391/149‑1 (MQ=255)

                                                                                                                                       cGCCTCTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGCTTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGa  >  2:965314/1‑149 (MQ=255)

CTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGA  >  NC_000913_3_hsa_tpiA/3486257‑3486540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A