Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F94 I1 R1	22	49.8	1741778	94.3%	1642496	141.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	3,089,163	G→C	V294L (GTG→CTG)	galP →	D‑galactose transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	3,089,163	0	G	C	95.2%	128.3 / 0.2	42	V294L (GTG→CTG)	galP	D‑galactose transporter
Reads supporting (aligned to +/- strand): ref base G (1/1); new base C (18/22); total (19/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.86e-01

ACAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCG  >  NC_000913_3_hsa_tpiA/3089017‑3089304

aCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGCACCCGCGGGTTACAGCAATCCTAACGAGCAAATGTGGGGGACCCTg                                                                                                                                             <  2:646144/149‑1 (MQ=255)

  aGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCCTGAt                                                                                                                                           >  1:430538/1‑149 (MQ=255)

   gCAACTGCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCCGGTAATGCAGCAATTCGCCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGCACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCCTGAtt                                                                                                                                          <  2:831532/149‑1 (MQ=255)

      aCTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCCTGATTGTc                                                                                                                                       <  2:12758/149‑1 (MQ=255)

           cgctgggcgGTGTTCCTTGGCGGACTGTTGCAGGTAATGCAGTAATTCACTGGCATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGGGGGTTATACTAACACTACCGTGCAAATGTGGGGGACCCTGATTGTCGGCCt                                                                                                                                  <  2:361408/143‑1 (MQ=255)

               gcgcgGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCCTGATTGTCGGCCTGAcc                                                                                                                              >  1:761349/1‑149 (MQ=255)

                     tgtTCCTTGGCGTACTGTTGGAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCCTGATTGTCGGCCTGACCAACTTa                                                                                                                        <  2:495195/149‑1 (MQ=255)

                        tCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCCTGATTGTCGGCCTGACCAACGTACtt                                                                                                                     <  1:749935/149‑1 (MQ=255)

                                                 gcagcaATTCACCGTGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCCTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCttg                                                                                            <  1:444065/149‑1 (MQ=255)

                                                  cagcaATTCACCGGGATGAACGTCCTCGTGCAACACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCCTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCttgt                                                                                           >  2:840627/1‑149 (MQ=255)

                                                     caATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTAGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCCTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGa                                                                                        >  1:574322/1‑149 (MQ=255)

                                                            ccGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCCTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTgg                                                                                 <  2:522636/149‑1 (MQ=255)

                                                               ggATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTAATACCAACACTACCGAGCAAATGTGGGTGACCCTGATTGTACGCCTGACCAACGTACGTTCAACATTTATAGCAATCTGCCTTGTTGCCCGCTgtaga                                                                              >  2:362808/1‑145 (MQ=255)

                                                                gATGAACGTCATCATGGATTACGCGCCTAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAACTGTGGGGGACCCTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTACCGCAATCGGCCTTGTTGACCGCTGGGGAc                                                                             <  1:470014/149‑1 (MQ=255)

                                                                  tGAACGCCATCATGGATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCCTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGt                                                                           <  1:809894/149‑1 (MQ=255)

                                                                    aaCGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCCTGATTGTCGGCCTGACCAACGTACTTGCCCCCTTTATCGCAATCGGCCTTGTTGACGGCTGGGGACGTaa                                                                         >  1:839466/1‑149 (MQ=255)

                                                                    aaCGGCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCCTGCTTGTCGGCCTGACCGACGAACTTGCCACCTTTATCGCAGTCGGCCTTATTAACCGCTGGGGGCGtca                                                                         >  2:68638/1‑147 (MQ=255)

                                                                              tGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCCTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCaacgcta                                                               >  1:778547/1‑149 (MQ=255)

                                                                                 aTTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACACTGATTGCCGGCCTGACCCACGTACTTGCCCCCTGTATCGCAATCCGCCTTGTTGACCGCTGCGGACCTACACCaacgctaacg                                                            >  2:138977/1‑149 (MQ=255)

                                                                                          cGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCCTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTc                                                   >  1:393457/1‑149 (MQ=255)

                                                                                             aaaccctCGAACTTGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCCTGATTGTCGGCCTGACCAACGTACTTGCCACCTCCATAGCATTCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTg                                                <  1:248181/143‑1 (MQ=255)

                                                                                               aTCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCCTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGt                                              <  2:430538/149‑1 (MQ=255)

                                                                                                tCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCCTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTg                                             <  1:319270/149‑1 (MQ=255)

                                                                                                 cTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCCTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGa                                            >  2:502922/1‑149 (MQ=255)

                                                                                                     gAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCCTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGAt                                           <  2:710612/146‑1 (MQ=255)

                                                                                                     gAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCCTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGAt                                           >  1:710612/1‑146 (MQ=255)

                                                                                                         tcgCGGGTTATCCCAACACTACCGAGCAAATGTGGGGGACCCTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGgctgct                                    <  1:749258/147‑1 (MQ=255)

                                                                                                               gTTATACCAACACTACCGAGCAAATGTGGGGGACCCTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAACCAACCCCGTTGACCGCGGGGGACGTAAACCAACGCTAACGCGGGGCATCCTGGGGGTGGCTGCTGGCATg                              >  1:202953/1‑149 (MQ=255)

                                                                                                                ttATACCAACACTACCGAGCAAATGTGGGGGACCCTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTTGTGATGGCTGCTGGCATgg                             <  2:734406/149‑1 (MQ=255)

                                                                                                                  ataCCAACACTACCGAGCAAATGTGGGGGACCCTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGc                           <  1:422967/149‑1 (MQ=255)

                                                                                                                      cAACACTACCGAGCAAATGTGGGGGACCCTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTAc                       >  1:433434/1‑149 (MQ=255)

                                                                                                                         cacTCCCGTGTAAATGTGGGTGACTCTCATGGGCGGCCTGACCAACGTACTGGGCACCTTTATCGCAATCGGCCTTGTTGAGCGCTGGGCACGTAAACCAACGCTAACGGTGGGCTTCCTGGTGATGGCTTCTGGCATGGGCGTACTCg                    <  2:641824/149‑1 (MQ=255)

                                                                                                                            tACCGAGCAAATGTGGGGGACCCTGATTGTCGGCCTGCCCAACGTACTTGCCACCTTTATCGCAATCGGACTTATTCAACGCTGGAGACGTAAACCAACGCTAACGCTGGGCTGCCTAGGTATGGCTGCTGGCATGGGCGGAccccgtt                 >  2:352693/1‑143 (MQ=255)

                                                                                                                            tACCGAGCAAATGTGGGGGACCCTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTa                 <  2:778547/149‑1 (MQ=255)

                                                                                                                            tACCGAGCAAATGTGGGGGACCCCGCTTGTCGGCCTGACCACCGTAACGTCTACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTa                 <  1:417813/149‑1 (MQ=255)

                                                                                                                              ccGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAAc                                                                       <  1:273315/93‑1 (MQ=255)

                                                                                                                              ccGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAAc                                                                       >  2:273315/1‑93 (MQ=255)

                                                                                                                               cGAGCAAATGTGGGGGACCCTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACaa              >  2:500769/1‑149 (MQ=255)

                                                                                                                                   cAAATGTGGGGGACCCTGATTGTCGGCCTGACCAACGTCCTTGCCACCTTTATCGCAATCGGCCTTGTTGCCCGCTGGGGACGTAAACCAACGCTAAGGCTCGGCTTCCGGGTGATGACTGCTGGAATGGGGGGAGTCGGTagcaggtg          >  2:237691/1‑142 (MQ=255)

                                                                                                                                           gggtACCCTGATTGTCGGCCTGACCATCGTACTTGCCGCCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGCGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCg  <  2:317711/149‑1 (MQ=255)

                                                                                                                                           ggggACCCTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCg  <  1:130883/149‑1 (MQ=255)

                                                                                                                                           ggggACCCTGATTGGCGGCCTGACGAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGGAAGAGCGTACTCGGTACAATGATGCATATCg  >  1:752933/1‑149 (MQ=255)

ACAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCG  >  NC_000913_3_hsa_tpiA/3089017‑3089304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A