Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F412 I2 R1 70 105.0 5244496 94.3% 4945559 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 681,304 T→C W365R (TGG→CGG)  djlC → J domain‑containing HscC co‑chaperone; Hsc56

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913681,3040TC99.1% 365.6 / ‑4.5 107W365R (TGG→CGG) djlCJ domain‑containing HscC co‑chaperone; Hsc56
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (50/56);  total (51/56)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01

Only 100 of 114 total aligned reads displayed.

CGGCTGTTATGGGCGGAGATTTTACATAGCGGAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGTGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCC  >  NC_000913/681208‑681398
                                                                                                |                                                                                              
cgGCTGTTATGGGCGGAGATTTTACATAGCGGAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCgcggt                                                                                             >  2:423887/1‑100 (MQ=255)
cgGCTGTTATGGGCGGAGATTTTACATAGCGGAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCgcggt                                                                                             >  1:1729989/1‑100 (MQ=255)
cgGCTGTTATGGGCGGAGATTTTACATAGCGGAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCgcggt                                                                                             >  1:1727271/1‑100 (MQ=255)
cgGCTGTTATGGGCGGAGATTTTACATAGCGGAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCgcggt                                                                                             >  1:2623907/1‑100 (MQ=255)
  gCTGTTATGGGCGGAGATTTTACATAGCGGAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGcggtgg                                                                                           <  2:2229583/100‑1 (MQ=255)
  gCTGTTATGGGCGGAGATTTTACATAGCGGAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGcggtgg                                                                                           >  2:592966/1‑100 (MQ=255)
  gCTGTTATGGGCGGAGATTTTACATAGCGGAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGcggtgg                                                                                           <  1:2425112/100‑1 (MQ=255)
   cTGTTATGGGCGGAGATTTTACATAGCGGAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGa                                                                                          <  1:2425883/100‑1 (MQ=255)
   cTGTTATGGGCGGAGATTTTACATAGCGGAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGa                                                                                          >  1:1240168/1‑100 (MQ=255)
   cTGTTATGGGCGGAGATTTTACATAGCGGAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGa                                                                                          >  1:1240421/1‑100 (MQ=255)
      ttATGGGCGGAGATTTTACATAGCGGAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAAt                                                                                       <  1:1561034/100‑1 (MQ=255)
       tATGGGCGGAGATTTTACATAGCGGAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATc                                                                                      <  1:1850768/100‑1 (MQ=255)
       tATGGGCGGAGATTTTACATAGCGGAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATc                                                                                      <  2:1918096/100‑1 (MQ=255)
        aTGGGCGGAGATTTTACATAGCGGAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCg                                                                                     >  1:493519/1‑100 (MQ=255)
         tGGGCGGAGATTTTACATAGCGGAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCga                                                                                    >  1:1501916/1‑100 (MQ=255)
         tGGGCGGAGATTTTACATAGCGGAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCACGGGCGCGGTGGAAATCga                                                                                    <  2:715626/100‑1 (MQ=255)
          gggCGGAGATTTTACATAGCGGAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCgag                                                                                   <  1:32231/100‑1 (MQ=255)
          gggCGGAGATTTTACATAGCGGAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCgag                                                                                   <  1:1430192/100‑1 (MQ=255)
          gggCGGAGATTTTACATAGCGGAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCgag                                                                                   >  1:117298/1‑100 (MQ=255)
          gggCGGAGATTTTACATAGCGGAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCgag                                                                                   <  1:2323114/100‑1 (MQ=255)
           ggCGGAGATTTTACATAGCGGAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCgaga                                                                                  >  1:2185204/1‑100 (MQ=255)
            gCGGAGATTTTACATAGCGGAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGagag                                                                                 <  1:554500/100‑1 (MQ=255)
             cGGAGATTTTACATAGCGGAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGt                                                                                >  1:2595534/1‑100 (MQ=255)
             cGGAGATTTTACATAGCGGAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGt                                                                                >  2:92215/1‑100 (MQ=255)
               gagaTTTTACATAGCGGAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCg                                                                              <  2:673486/100‑1 (MQ=255)
                  aTTTTACATAGCGGAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTa                                                                           <  2:1769923/100‑1 (MQ=255)
                  aTTTTACATAGCGGAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTa                                                                           <  1:2546719/100‑1 (MQ=255)
                   ttttACATAGCGGAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAg                                                                          <  1:2091332/100‑1 (MQ=255)
                     ttACATAGCGGAAAATTACTGCCGTTAGGTCAATTGTTTATTGAGTAGTTATTCGTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCAGTAGCg                                                                        <  2:2339254/100‑1 (MQ=255)
                        cATAGCGGAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCgaga                                                                     >  1:2449247/1‑100 (MQ=255)
                          tAGCGGAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACa                                                                   >  1:740146/1‑100 (MQ=255)
                           aggcgAAAATTACCGCCGTTAGGTCAAACCTTTATTGGGGCGTTATGCGTCAAGCGCAAAGCAGGGGCCCGGTGGAAATCGAGAGTCGGTAGCGAGACag                                                                  <  2:2547573/96‑1 (MQ=255)
                           aGCGGAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACag                                                                  >  1:584109/1‑100 (MQ=255)
                           aGCGGAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACag                                                                  <  1:2218546/100‑1 (MQ=255)
                            gCGGAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATTGAGAGTCGGTAGCGAGACaga                                                                 >  2:2416432/1‑100 (MQ=255)
                             cGGAAAATTATCGCCGTTAGGACAATCGTCTATTGCGTCGCTATTCTTCAAGCGCAAAGCATGGGCACGGTGGAGATCTAGAGTCGGTAGCGAGACagcg                                                                >  2:2453964/1‑98 (MQ=255)
                             cGGAAAACTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATGCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACagag                                                                <  1:2432284/100‑1 (MQ=255)
                              ggAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGc                                                               >  1:1820579/1‑100 (MQ=255)
                              ggAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGc                                                               <  1:1606880/100‑1 (MQ=255)
                               gAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCa                                                              <  1:432964/100‑1 (MQ=255)
                               gAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCa                                                              >  2:2069517/1‑100 (MQ=255)
                                 aaaTTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAg                                                            >  1:1610159/1‑100 (MQ=255)
                                   aTTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGAt                                                          <  1:2379401/100‑1 (MQ=255)
                                        cGCCGTTAGGTCAATCGGTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCAcc                                                     <  1:2555209/100‑1 (MQ=255)
                                         gCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCg                                                    >  1:2018443/1‑100 (MQ=255)
                                         gCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCg                                                    >  1:2018412/1‑100 (MQ=255)
                                          ccGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCGAGATTCACCGt                                                   >  2:1101521/1‑100 (MQ=255)
                                           cGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGtt                                                  <  1:1198405/100‑1 (MQ=255)
                                             ttAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTcc                                                <  1:283734/100‑1 (MQ=255)
                                              tAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCt                                               >  2:423765/1‑100 (MQ=255)
                                               aGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTg                                              <  2:714612/100‑1 (MQ=255)
                                               aGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTg                                              <  2:1390489/100‑1 (MQ=255)
                                               aGGTCAATCGTTTATTGAGTCGTTATTCTGCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGAGAGAGCAAGATGCACCGTTCCTg                                              <  2:444115/100‑1 (MQ=255)
                                                 gTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGa                                            >  2:278564/1‑100 (MQ=255)
                                                 gTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGa                                            >  1:575298/1‑100 (MQ=255)
                                                   cAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGAtt                                          >  1:2390429/1‑100 (MQ=255)
                                                     aTCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTg                                        <  2:2099386/100‑1 (MQ=255)
                                                     aTCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTg                                        <  1:1254096/100‑1 (MQ=255)
                                                     aTCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTg                                        <  1:1075613/100‑1 (MQ=255)
                                                     aTCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTg                                        <  1:1501896/100‑1 (MQ=255)
                                                     aTCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTg                                        >  1:1993257/1‑100 (MQ=255)
                                                        gTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTAt                                     >  1:363863/1‑100 (MQ=255)
                                                         tttATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATc                                    <  1:387834/100‑1 (MQ=255)
                                                         tttATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATc                                    <  2:315072/100‑1 (MQ=255)
                                                           tATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCgg                                  >  1:865828/1‑100 (MQ=255)
                                                              tGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTa                               >  2:1664157/1‑100 (MQ=255)
                                                                 gTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCg                            >  2:2563215/1‑100 (MQ=255)
                                                                 gTCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCg                            <  2:2345619/100‑1 (MQ=255)
                                                                  tCGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCgg                           >  2:830501/1‑100 (MQ=255)
                                                                   cGTTATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGa                          >  1:296721/1‑100 (MQ=255)
                                                                      tATTCTTCCAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGaaga                       >  2:1508045/1‑98 (MQ=255)
                                                                      tATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACa                       >  2:1476533/1‑100 (MQ=255)
                                                                      tATTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACa                       >  1:1307704/1‑100 (MQ=255)
                                                                       aTTCTTCAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAg                      <  1:1161892/100‑1 (MQ=255)
                                                                        ttcttcAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAgg                     >  2:412644/1‑100 (MQ=255)
                                                                         tcttcAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGgt                    <  2:793528/100‑1 (MQ=255)
                                                                         tcttcAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGgt                    >  1:1578271/1‑100 (MQ=255)
                                                                         tcttcAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGgt                    <  2:1674644/100‑1 (MQ=255)
                                                                           ttcAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGgtag                  >  2:1723511/1‑100 (MQ=255)
                                                                            tcAAGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGgtagt                 <  1:906764/100‑1 (MQ=255)
                                                                              aaGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTAc               <  1:1867953/100‑1 (MQ=255)
                                                                               aGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACt              >  1:1638339/1‑100 (MQ=255)
                                                                               aGCGCAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACt              <  2:585740/100‑1 (MQ=255)
                                                                                 cgcAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGTGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTg            <  2:1369127/100‑1 (MQ=255)
                                                                                 cgcAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTg            >  2:1505566/1‑100 (MQ=255)
                                                                                 cgcAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTg            >  2:299893/1‑100 (MQ=255)
                                                                                 cgcAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTg            <  1:339643/100‑1 (MQ=255)
                                                                                   cAAAGCATGGGCGCGGTGGAAATGGAGATTCGGTAGCGAGACAGAGTAAGATTAATCTTACCTGGATGTGTATCGGGTAGCGGAACAGGTAGTATTTGaa          >  2:382633/1‑100 (MQ=255)
                                                                                   cAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCTAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGaa          <  2:2357292/100‑1 (MQ=255)
                                                                                   cAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGaa          >  1:495917/1‑100 (MQ=255)
                                                                                   cAAAGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGaa          <  1:609422/100‑1 (MQ=255)
                                                                                     aaGCATGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGc        <  2:2137956/100‑1 (MQ=255)
                                                                                         aTGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGttt    >  1:648536/1‑100 (MQ=255)
                                                                                          tGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTc   <  1:1808405/100‑1 (MQ=255)
                                                                                          tGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTc   >  2:16320/1‑100 (MQ=255)
                                                                                          tGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTc   <  1:2058417/100‑1 (MQ=255)
                                                                                          tGGGCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTc   >  2:16775/1‑100 (MQ=255)
                                                                                           gggCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTcc  <  2:1481228/100‑1 (MQ=255)
                                                                                           gggCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTcc  <  2:1483547/100‑1 (MQ=255)
                                                                                           gggCGCGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTcc  <  2:726199/100‑1 (MQ=255)
                                                                                                |                                                                                              
CGGCTGTTATGGGCGGAGATTTTACATAGCGGAAAATTATCGCCGTTAGGTCAATCGTTTATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGTGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCC  >  NC_000913/681208‑681398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A