Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F412 I2 R1 70 105.0 5244496 94.3% 4945559 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 756,515 C→T G203G (GGC→GGT)  sdhA → succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913756,5150CT97.1% 333.8 / ‑0.1 106G203G (GGC→GGT) sdhAsuccinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base T (47/54);  total (49/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.38e-01

Only 100 of 110 total aligned reads displayed.

AAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGAT  >  NC_000913/756418‑756611
                                                                                                 |                                                                                                
acaaCCAGGATGGCGCGGTGGTGGGTTGTCCCGCACTGTGCACCGAAACCGCTGAAGTGGTTTATTTCCAAGCCCGCGCTACCGTGCTGGCGACtggtgg                                                                                                <  2:251619/98‑1 (MQ=255)
aaaaCCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACtggtgg                                                                                                <  1:493170/100‑1 (MQ=255)
 aagccAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGa                                                                                               <  2:1378231/97‑1 (MQ=255)
 aaaCCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGTGACTGGTGGa                                                                                               <  1:1938220/100‑1 (MQ=255)
 aaaCCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGa                                                                                               <  1:2356627/100‑1 (MQ=255)
  aaCCAGGATGGCGCGGTGGTGGGTTGTACCGCAGTGGGCATCGACAACGGAGAAATGGGTTAATTGAAAGCCGGCGCTACCGTGCTGGCGACTGGTGGAg                                                                                              >  2:593324/1‑100 (MQ=255)
  aaCCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAg                                                                                              >  1:1611787/1‑100 (MQ=255)
  aaCCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAg                                                                                              <  1:1244009/100‑1 (MQ=255)
   aCCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGc                                                                                             >  2:1404124/1‑100 (MQ=255)
     cAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAg                                                                                           <  1:1942344/100‑1 (MQ=255)
       ggATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAggg                                                                                         <  2:2314501/100‑1 (MQ=255)
       ggATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAggg                                                                                         <  1:2353470/100‑1 (MQ=255)
        gATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGc                                                                                        <  1:83696/100‑1 (MQ=255)
         aTGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGcn                                                                                       <  1:2104945/100‑2 (MQ=255)
         aTGGCGCGGTGGTGGGTTGTACCGCACCGTGCATTGAAACTGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCg                                                                                       <  2:2481906/100‑1 (MQ=255)
          tGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGt                                                                                      >  1:1781103/1‑100 (MQ=255)
          tGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGt                                                                                      >  2:1474514/1‑100 (MQ=255)
           ggCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTa                                                                                     >  1:1502389/1‑100 (MQ=255)
             cgcgGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTAtt                                                                                   <  2:570041/100‑1 (MQ=255)
             cgcgGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTAtt                                                                                   <  1:1104070/100‑1 (MQ=255)
              gcgGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTAttt                                                                                  <  1:799282/100‑1 (MQ=255)
                 gtggtgGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATc                                                                               <  1:96263/100‑1 (MQ=255)
                 gtggtgGGTAGTACCGCACTGAGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACAGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATc                                                                               <  2:2092422/100‑1 (MQ=255)
                   ggtggGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCan                                                                             <  1:29125/100‑2 (MQ=255)
                   ggtggGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAg                                                                             >  1:1126688/1‑100 (MQ=255)
                   ggtggGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAg                                                                             <  2:2122876/100‑1 (MQ=255)
                    gtggGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGt                                                                            >  2:1849583/1‑100 (MQ=255)
                     tggGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTc                                                                           >  1:2368782/1‑100 (MQ=255)
                      gggTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTcc                                                                          <  1:1473126/100‑1 (MQ=255)
                       ggTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTcca                                                                         >  1:1105262/1‑100 (MQ=255)
                         ttGTACCGCACTGTGCATCGAACCCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTCCCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTccacc                                                                       <  2:184915/100‑1 (MQ=255)
                         ttGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTccacc                                                                       >  1:926337/1‑100 (MQ=255)
                         ttGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTccacc                                                                       <  1:1186059/100‑1 (MQ=255)
                           gTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTccaccac                                                                     >  1:161549/1‑100 (MQ=255)
                            tACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTccaccacc                                                                    <  1:2569959/100‑1 (MQ=255)
                            tACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTccaccacc                                                                    <  1:2645210/100‑1 (MQ=255)
                            tACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTccaccacc                                                                    <  1:2193301/100‑1 (MQ=255)
                            tACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTccaccacc                                                                    <  1:2160543/100‑1 (MQ=255)
                             aCCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTccaccacca                                                                   >  2:1624055/1‑100 (MQ=255)
                             aCCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTccaccacca                                                                   >  1:2614808/1‑100 (MQ=255)
                              ccGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCaa                                                                  >  2:536301/1‑100 (MQ=255)
                              ccGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCaa                                                                  <  2:2439813/100‑1 (MQ=255)
                              ccGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCaa                                                                  >  2:515748/1‑100 (MQ=255)
                              ccGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCaa                                                                  <  2:1510525/100‑1 (MQ=255)
                                gCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACg                                                                <  1:420932/100‑1 (MQ=255)
                                    tgtgCCCCGAAACGGGTGAAGTGCTTTAGTTGAAAGCCGGCGCTAGCGTGCTGGCGACTGGGGGAGCAGGGCTTATTTAGCAGTCGACCACCAACGCCca                                                            <  2:2419832/100‑1 (MQ=255)
                                    tgtgCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCca                                                            <  1:1471735/100‑1 (MQ=255)
                                    tgtgCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCca                                                            >  2:2091517/1‑100 (MQ=255)
                                        cATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACAtt                                                        <  1:1118820/100‑1 (MQ=255)
                                        cATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACAtt                                                        >  1:838503/1‑100 (MQ=255)
                                         aTCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTTCCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTa                                                       <  2:1228529/100‑1 (MQ=255)
                                         aTCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTa                                                       <  2:224073/100‑1 (MQ=255)
                                          tCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTaa                                                      >  1:852320/1‑100 (MQ=255)
                                           cGACACCGGTGAAGTGGTTTCTTACAAAACCCGCGCTACCGTGCTGGCGACGGGTAGCGCGGGGCGTACTTATCAGTCCACCACCAACGCCCACATTAac                                                     >  2:1465310/1‑100 (MQ=255)
                                           cGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAac                                                     <  1:244597/100‑1 (MQ=255)
                                             aaaCCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAacac                                                   >  2:1762151/1‑100 (MQ=255)
                                              aaCCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACAcc                                                  >  1:977303/1‑100 (MQ=255)
                                              aaCCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACAcc                                                  >  1:1796451/1‑100 (MQ=255)
                                              aaCCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACAcc                                                  >  1:1944987/1‑100 (MQ=255)
                                                 cGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGc                                               >  1:550350/1‑100 (MQ=255)
                                                   gTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGa                                             >  2:2102100/1‑100 (MQ=255)
                                                     gAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACg                                           >  1:1160935/1‑100 (MQ=255)
                                                       aGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGgt                                         >  1:2603326/1‑100 (MQ=255)
                                                        gTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGgtg                                        <  2:949342/100‑1 (MQ=255)
                                                        gTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGgtg                                        <  1:1536094/100‑1 (MQ=255)
                                                           gTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTCACACCGGCGACGGTGTCg                                     >  2:1039181/1‑100 (MQ=255)
                                                           gTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCg                                     >  1:977345/1‑100 (MQ=255)
                                                            tttATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCgg                                    <  2:448478/100‑1 (MQ=255)
                                                            tttATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCgg                                    <  2:448643/100‑1 (MQ=255)
                                                            tttATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCgg                                    <  2:2291529/100‑1 (MQ=255)
                                                              tATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCa                                  >  2:2375453/1‑100 (MQ=255)
                                                              tATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCATCACCAACGCCCACATTAACACCGGCGACGGTGTCGGcc                                  <  2:2580602/100‑2 (MQ=255)
                                                               aTTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCAt                                 >  1:608513/1‑100 (MQ=255)
                                                                tttCAAAGCCCGCGCTCCCCTGCTGGCGACTGGTGCAGCAGGGCATATTTATCCGTCCACCACCAACGCCCAAATTAAAACCGAAAACGGTGTCGGCATg                                <  2:1403946/100‑1 (MQ=255)
                                                                tttCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATg                                <  1:107098/100‑1 (MQ=255)
                                                                tttCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATg                                >  1:2022833/1‑100 (MQ=255)
                                                                 ttCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGTATgg                               <  1:1495117/100‑1 (MQ=255)
                                                                 ttCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATgg                               <  2:501786/100‑1 (MQ=255)
                                                                  tCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGc                              <  2:1441194/100‑1 (MQ=255)
                                                                  tCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGc                              <  1:29912/100‑1 (MQ=255)
                                                                  tCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGc                              <  1:1876771/100‑1 (MQ=255)
                                                                  tCAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGc                              <  2:265282/100‑1 (MQ=255)
                                                                   cAAAGCCCGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCt                             >  1:1947609/1‑100 (MQ=255)
                                                                         ccGCGCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGt                       >  1:2316264/1‑100 (MQ=255)
                                                                          cgcgCTACCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTg                      >  1:1022918/1‑100 (MQ=255)
                                                                                aCCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCg                >  2:656507/1‑100 (MQ=255)
                                                                                aCCGTGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCg                >  1:473037/1‑100 (MQ=255)
                                                                                    tGCTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTAcc            <  2:2382523/100‑1 (MQ=255)
                                                                                      cTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCgg          <  2:981490/100‑1 (MQ=255)
                                                                                      cTGGCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCgg          <  1:1866239/100‑1 (MQ=255)
                                                                                        ggCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTg        <  1:2043624/100‑1 (MQ=255)
                                                                                        ggCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTg        <  1:1404970/100‑1 (MQ=255)
                                                                                         gCGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACGCCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGc       <  1:44783/100‑1 (MQ=255)
                                                                                          cGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTGCCGGTGCa      >  2:1913225/1‑100 (MQ=255)
                                                                                          cGACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCa      >  2:223860/1‑100 (MQ=255)
                                                                                           gACTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAg     >  1:508389/1‑100 (MQ=255)
                                                                                             cTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGa   >  2:2541890/1‑100 (MQ=255)
                                                                                             cTGGTGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGa   >  1:1115385/1‑100 (MQ=255)
                                                                                              tggtggAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGat  <  1:1988710/100‑1 (MQ=255)
                                                                                              tggtggAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGat  >  1:2052265/1‑100 (MQ=255)
                                                                                                 |                                                                                                
AAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGAT  >  NC_000913/756418‑756611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A