Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F412 I2 R1
|
70 |
105.0 |
5244496 |
94.3% |
4945559 |
100.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,108,247 |
A→G |
I232T (ATT→ACT) |
opgC ← |
OPG biosynthetic transmembrane succinyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,108,247 | 0 | A | G | 98.6%
| 232.3
/ NA
| 70 | I232T (ATT→ACT) | opgC | OPG biosynthetic transmembrane succinyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (31/38); minor base T (0/1); total (31/39) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATAGCGCTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAATGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATGGGCGGATA > NC_000913/1108150‑1108341
|
aTAGCGCTTGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTg < 2:1413921/100‑1 (MQ=255)
gcgcTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGaaa > 2:863482/1‑100 (MQ=255)
gcgcTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGaaa > 2:1845992/1‑100 (MQ=255)
cgcTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAg > 2:987773/1‑100 (MQ=255)
cgcTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAg < 2:1177698/100‑1 (MQ=255)
cgcTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAg < 1:1738039/100‑1 (MQ=255)
gcTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGc > 1:86852/1‑100 (MQ=255)
ggTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAg > 1:404361/1‑100 (MQ=255)
gAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTgcg > 1:2435190/1‑100 (MQ=255)
aGTAAATAAGCGACAAACGCCAATGCTTCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGACTTTAAGATGAGGGAAAGTGAAAGACACTgcgc > 2:313128/1‑100 (MQ=255)
aGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTgcgc > 1:915966/1‑100 (MQ=255)
aGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTgcgc > 1:885488/1‑100 (MQ=255)
gTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGcc < 1:855994/100‑1 (MQ=255)
tAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCg > 2:2350197/1‑100 (MQ=255)
aaTAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAg < 2:2234657/100‑1 (MQ=255)
aTAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAgg > 2:242297/1‑100 (MQ=255)
aTAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAgg < 1:1396649/100‑1 (MQ=255)
tAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGa < 2:1476979/100‑1 (MQ=255)
aaGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGAt < 2:63155/100‑1 (MQ=255)
aGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATa > 1:1194538/1‑100 (MQ=255)
gACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAg < 2:79910/100‑1 (MQ=255)
aCAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGAGAAAGa < 2:1814210/100‑1 (MQ=255)
aaaCGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAAc < 2:154673/100‑1 (MQ=255)
aaCGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACg > 1:2617970/1‑100 (MQ=255)
aaCGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACg < 2:1871412/100‑1 (MQ=255)
aCGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACgg > 1:94938/1‑100 (MQ=255)
cGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGTCAGTGCGCCGAGGATAAAGAACGGc < 2:297528/100‑1 (MQ=255)
ccAATGCTGGTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCaa < 2:1330100/100‑1 (MQ=255)
ccAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCaa < 2:2410396/100‑1 (MQ=255)
ccAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCaa < 1:2323276/100‑1 (MQ=255)
tgctgcAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAAc < 1:2223125/100‑1 (MQ=255)
ctgcAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAg > 1:573121/1‑100 (MQ=255)
tgcAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGGACGGCACATAAAACAGc > 2:12176/1‑100 (MQ=255)
gcAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCg > 1:2019442/1‑100 (MQ=255)
aGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGttt > 1:989621/1‑100 (MQ=255)
aGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGttt < 2:227563/100‑1 (MQ=255)
gTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGCTGAGGGAAAGTGAACGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCa < 1:1724036/100‑1 (MQ=255)
gTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCa > 1:363078/1‑100 (MQ=255)
tACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCAt < 1:1563172/100‑1 (MQ=255)
aaaGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATg < 2:2242004/98‑1 (MQ=255)
aCAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATg > 2:1079972/1‑100 (MQ=255)
aCAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATg < 2:1267844/100‑1 (MQ=255)
gaGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGa > 2:515387/2‑100 (MQ=255)
aGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGAc < 2:1176791/100‑1 (MQ=255)
gaCGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAAt > 1:2483205/1‑100 (MQ=255)
gaCGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAAt < 1:582601/100‑1 (MQ=255)
cGTGGTAAACAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAAc < 1:1362635/100‑1 (MQ=255)
cGTGGTAAACAAGGCTTTAAGAAGAGGGAAAGTCAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTACATGACAATAAAATTGaag > 2:264967/1‑99 (MQ=255)
aaaCAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCa > 1:585019/1‑100 (MQ=255)
aaaCAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCa > 1:37824/1‑100 (MQ=255)
aaCAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCAt < 1:765935/100‑1 (MQ=255)
ataaGGCTTTAAGATGAGGAAAAGTGAACGCCAGTGCGCCGAGGATAAAGAACGGCAATTAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCAtt < 2:2072551/98‑1 (MQ=255)
cAAGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATCAAATTGAACATGCCATTa > 1:108579/1‑100 (MQ=255)
cAAGGCTTTAAGAAGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAAAAGCCAATAACACAGCGTTAGCATGACAATAAAATTGAAAATCCCATTa > 2:2147735/1‑100 (MQ=255)
aaGGCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTAc > 1:278627/1‑100 (MQ=255)
ggCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGGCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGATAATAAAATTGAACATGCCATTACTc < 2:2073877/100‑1 (MQ=255)
ggCTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTc < 2:1191261/100‑1 (MQ=255)
tcgctAAGATAAGGGAAATTGACAGACACTGCGCCGAGTATAAAGAACGGCAAATATAACATCGTTTGCATGACAATAAAATTGAACATGCCATTACTCa < 1:2135699/96‑1 (MQ=255)
cTTTAAGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAg > 1:253442/1‑100 (MQ=255)
aaGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATg > 1:1519439/1‑100 (MQ=255)
aaGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATg < 1:1386222/100‑1 (MQ=255)
aaGATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATg < 1:1385206/100‑1 (MQ=255)
aaGATCAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAAAAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATg > 2:2282124/1‑100 (MQ=255)
gATGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATggg < 1:2626710/100‑1 (MQ=255)
tGAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATGGGCg < 2:1512409/100‑1 (MQ=255)
gAGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATGGGCgg < 2:85551/100‑1 (MQ=255)
aGGGAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATGGGCGGa < 2:2528439/100‑1 (MQ=255)
aGGGAAAGTGAAAGCCAGGGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATATAATTGAACATGCCATTACTCAGAATGGGCGGa < 2:2039932/100‑1 (MQ=255)
ggAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATGGGCGGATa < 1:1910255/100‑1 (MQ=255)
ggAAAGTGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATGGGCGGATa < 1:2472337/100‑1 (MQ=255)
|
ATAGCGCTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAATGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATGGGCGGATA > NC_000913/1108150‑1108341
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
