Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F412 I2 R1 70 105.0 5244496 94.3% 4945559 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,900,732 T→C E285G (GAA→GGA)  yoaE ← putative membrane protein/conserved protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,900,7320TC98.0% 340.5 / ‑3.0 100E285G (GAA→GGA) yoaEputative membrane protein/conserved protein
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (44/54);  total (45/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 103 total aligned reads displayed.

GATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCCATCAAAC  >  NC_000913/1900637‑1900825
                                                                                               |                                                                                             
gaTTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGtccttc                                                                                           <  1:1794302/100‑1 (MQ=255)
 aTTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGtccttct                                                                                          >  1:1505887/1‑100 (MQ=255)
   tAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGtccttcttc                                                                                        >  2:850483/1‑100 (MQ=255)
    aGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCg                                                                                       >  2:1128787/1‑100 (MQ=255)
    aGCGTCAACCCAGCTTAGTTCACCGGGCGGCGTCATGATCCGGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTGATCATGTAACGTCCTTCTTCg                                                                                       <  2:1084860/100‑1 (MQ=255)
     gCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCgg                                                                                      <  1:1846369/100‑1 (MQ=255)
     gCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCgg                                                                                      <  2:733862/100‑1 (MQ=255)
     gCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCgc                                                                                      >  1:701553/1‑99 (MQ=255)
       gTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCa                                                                                    <  2:864875/100‑1 (MQ=255)
        tCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCaa                                                                                   <  2:1497516/100‑1 (MQ=255)
         cAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCaaa                                                                                  <  1:1005112/100‑1 (MQ=255)
           aCCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCACATg                                                                                >  2:2578469/1‑100 (MQ=255)
           aCCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATg                                                                                <  1:739003/100‑1 (MQ=255)
           aCCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATg                                                                                <  1:2556219/100‑1 (MQ=255)
            cccAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCTTTAATCATGTAACGTCCTTCTTCGGCAAATGc                                                                               <  2:201379/100‑1 (MQ=255)
            cccAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGc                                                                               <  1:1773019/100‑1 (MQ=255)
             ccAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCa                                                                              >  1:1761417/1‑100 (MQ=255)
              cAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCAc                                                                             <  2:395278/100‑1 (MQ=255)
               aGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCAcc                                                                            <  2:783377/100‑1 (MQ=255)
               aGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCAcc                                                                            <  2:579055/100‑1 (MQ=255)
               aGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCAcc                                                                            >  2:162252/1‑100 (MQ=255)
                gCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCt                                                                           >  2:2520976/1‑100 (MQ=255)
                   tATTGCACCGCGCGGCGGCAGTATTCCTCGCAGAGAACGCGACGCCAGCGTCAGTACTCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTcc                                                                        <  2:1958681/100‑1 (MQ=255)
                    aTTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCg                                                                       >  2:1538904/1‑100 (MQ=255)
                    aTTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCg                                                                       >  2:1391234/1‑100 (MQ=255)
                     tttCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCgg                                                                      >  2:529759/1‑100 (MQ=255)
                       tCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGa                                                                    >  2:1659295/1‑100 (MQ=255)
                       tCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGa                                                                    >  1:726088/1‑100 (MQ=255)
                        cACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGat                                                                   >  2:503295/1‑100 (MQ=255)
                          ccGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCATATGCACCTTCCCGGatcg                                                                 <  1:2203969/100‑1 (MQ=255)
                          ccGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGatcg                                                                 >  1:2377349/1‑100 (MQ=255)
                          ccGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGatcg                                                                 <  2:1761117/100‑1 (MQ=255)
                           cgcgcgGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGatcgg                                                                <  1:2563822/100‑1 (MQ=255)
                           cgcgcgGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGatcgg                                                                <  2:47428/100‑1 (MQ=255)
                                 gcgTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGatcggcatcgg                                                          <  2:822114/100‑1 (MQ=255)
                                  cgTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGatcggcatcggc                                                         <  2:1009969/100‑1 (MQ=255)
                                   gTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGAtcggcatcggca                                                        >  2:935607/1‑100 (MQ=255)
                                     cATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCggcatcggcatc                                                      >  1:13579/1‑100 (MQ=255)
                                     cATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTGCGGCAAATGCACCTTCCGGGATCggcatcggcatc                                                      <  2:1049145/100‑1 (MQ=255)
                                      aTGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGgcatcggcatcg                                                     >  2:1666812/1‑100 (MQ=255)
                                       tGATCCCGCGCAGAGACCGCGACGCGAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGcatcggcatcgg                                                    <  2:1772768/100‑1 (MQ=255)
                                       tGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGcatcggcatcgg                                                    >  1:1534186/1‑100 (MQ=255)
                                       tGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGcatcggcatcgg                                                    >  2:2463352/1‑100 (MQ=255)
                                       tGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGcatcggcatcgg                                                    >  2:1210650/1‑100 (MQ=255)
                                        gATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCggg                                                   <  1:193797/100‑1 (MQ=255)
                                        gATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGCATCGGCATCGGTATCggg                                                   <  1:2203831/100‑1 (MQ=255)
                                         aTCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGt                                                  >  2:622593/1‑100 (MQ=255)
                                         aTCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGt                                                  <  1:1781216/100‑1 (MQ=255)
                                          tCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGtt                                                 >  1:863211/1‑100 (MQ=255)
                                          tCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGtn                                                 <  1:2099621/100‑2 (MQ=255)
                                           cccGCGCAGAGAGCGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTa                                                >  1:2274272/1‑100 (MQ=255)
                                           cccGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTa                                                >  2:846692/1‑100 (MQ=255)
                                           cccGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTa                                                <  1:1738786/100‑1 (MQ=255)
                                             cgcgCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATc                                              >  1:624256/1‑100 (MQ=255)
                                              gcgcAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCg                                             >  1:761731/1‑100 (MQ=255)
                                               cgcAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCgg                                            <  1:1542282/100‑1 (MQ=255)
                                                  agagAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCAt                                         <  1:490844/100‑1 (MQ=255)
                                                  agagAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCAt                                         <  1:70294/100‑1 (MQ=255)
                                                  agagAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCAt                                         >  1:543000/1‑100 (MQ=255)
                                                   gagaACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATc                                        >  1:2022662/1‑100 (MQ=255)
                                                    agaACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCg                                       <  1:709909/100‑1 (MQ=255)
                                                       aCGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGc                                    <  1:1648691/100‑1 (MQ=255)
                                                       aCGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGc                                    <  2:1604178/100‑1 (MQ=255)
                                                       aCGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCCTGc                                    <  2:2381994/100‑1 (MQ=255)
                                                        cgcgACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCt                                   >  2:1508609/1‑100 (MQ=255)
                                                        cgcgACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCt                                   >  1:1641988/1‑100 (MQ=255)
                                                          cgACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGa                                 >  1:1371428/1‑100 (MQ=255)
                                                          cgACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGa                                 <  1:1560351/100‑1 (MQ=255)
                                                          cgACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGa                                 >  2:859302/1‑100 (MQ=255)
                                                          cgACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGa                                 <  1:2567646/100‑1 (MQ=255)
                                                              gCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCTCCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACAt                             <  2:538057/100‑1 (MQ=255)
                                                              gCCAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACAt                             <  1:2483433/100‑1 (MQ=255)
                                                               ccAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCCTCGGGTTATCGGCATCGTGCTGAACAtt                            >  2:2164355/1‑100 (MQ=255)
                                                               ccAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACAtt                            <  1:1493554/100‑1 (MQ=255)
                                                                cAGCGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACATTg                           <  2:124982/100‑1 (MQ=255)
                                                                   cGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGcc                        >  1:26168/1‑100 (MQ=255)
                                                                   cGTCAGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGcc                        <  1:2002295/100‑1 (MQ=255)
                                                                   cGTCAGTACGCCGTTAATCATGTAACGTCCTGCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGcc                        <  1:1020782/100‑1 (MQ=255)
                                                                       aGTACGCCGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGTCATCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGCCTGAc                    >  2:642104/1‑100 (MQ=255)
                                                                            gCCGTTAATGATGTAACGTCCTGCTTCTGCAAATGCAGCTTTCTGGATCGGCATCGTCATCGGGTTATCGTCATCGTGCTGAACATTGGCGTTACGTTTc               <  2:2143188/100‑1 (MQ=255)
                                                                             ccGTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGCCTGACGTTTcc              <  2:2108829/100‑1 (MQ=255)
                                                                               gTTAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCg            >  1:616574/1‑100 (MQ=255)
                                                                                ttAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGc           <  2:1565799/100‑1 (MQ=255)
                                                                                 tAATCATGTAACGTTCTTCTTCGGCAAATGCACCCTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGcc          >  2:2088887/1‑100 (MQ=255)
                                                                                 tAATCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGcc          <  1:394107/100‑1 (MQ=255)
                                                                                  aaTCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGccc         <  1:2568108/100‑1 (MQ=255)
                                                                                  aaTCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGccc         <  2:1135041/100‑1 (MQ=255)
                                                                                   aTCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCCa        <  1:41015/100‑1 (MQ=255)
                                                                                   aTCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCCa        >  2:1564557/1‑100 (MQ=255)
                                                                                   aTCATGTAACGTCCTTCTTCGGCAAATGCAACTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCCa        >  1:508575/1‑100 (MQ=255)
                                                                                    tCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCCAt       >  2:1000697/1‑100 (MQ=255)
                                                                                    tCATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCCAt       <  1:1068792/100‑1 (MQ=255)
                                                                                     cATGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCCATc      <  1:1396287/100‑1 (MQ=255)
                                                                                      aTGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCCATCa     >  2:362770/1‑100 (MQ=255)
                                                                                      aTGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCCATCa     <  1:2335935/100‑1 (MQ=255)
                                                                                      aTGTAACGTCCTTCTACGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCCCTCa     >  2:1976115/1‑100 (MQ=255)
                                                                                       tGTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCCATCaa    <  2:1066374/100‑1 (MQ=255)
                                                                                        gTAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCCATCaaa   >  2:1593439/1‑100 (MQ=255)
                                                                                         tAACGTTCTTCTTCGGCAAATGCACCTTCCGGGATCGGCAGCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGCCTGACGTTGCCCGCCCATCAAAc  <  2:1848063/100‑1 (MQ=255)
                                                                                         tAACGTCCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCCATCAAAc  >  2:147993/1‑100 (MQ=255)
                                                                                               |                                                                                             
GATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCCATCAAAC  >  NC_000913/1900637‑1900825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A