Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F412 I2 R1 70 105.0 5244496 94.3% 4945559 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,968,770 G→A R199C (CGT→TGT)  cheR ← chemotaxis regulator, protein‑glutamate methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,968,7700GA98.9% 297.4 / ‑4.4 87R199C (CGT→TGT) cheRchemotaxis regulator, protein‑glutamate methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (46/40);  total (46/41)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01

TTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGAT  >  NC_000913/1968673‑1968866
                                                                                                 |                                                                                                
ttACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGacaca                                                                                                <  2:2015703/100‑1 (MQ=255)
 tACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGacacac                                                                                               >  2:1273841/1‑100 (MQ=255)
   cGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGacacacgc                                                                                             >  2:2040650/1‑100 (MQ=255)
   cGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGacacacgc                                                                                             >  1:804535/1‑100 (MQ=255)
    gACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACAcgcg                                                                                            >  2:333260/1‑100 (MQ=255)
      cAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTa                                                                                          <  2:19736/100‑1 (MQ=255)
       agaagaTCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTAc                                                                                         >  1:664927/1‑100 (MQ=255)
        gaagaTCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTAcc                                                                                        >  1:792916/1‑100 (MQ=255)
        gaagaTCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTAcc                                                                                        <  1:439585/100‑1 (MQ=255)
          agaTCGCATCAAACGGCCCTGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAg                                                                                      >  2:2462789/1‑100 (MQ=255)
             tCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGccc                                                                                   >  1:424343/1‑100 (MQ=255)
              cGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCt                                                                                  >  2:2533475/1‑100 (MQ=255)
               gCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCtt                                                                                 <  1:2321169/100‑1 (MQ=255)
                 aTCAAACGGCCCCGGCACGGTGTCCTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCa                                                                               <  2:980890/100‑1 (MQ=255)
                 aTCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCa                                                                               >  2:2171277/1‑100 (MQ=255)
                    aaaCGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGc                                                                            >  1:618646/1‑100 (MQ=255)
                     aaCGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCg                                                                           <  1:2549303/100‑1 (MQ=255)
                     aaCGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCg                                                                           <  1:1487782/100‑1 (MQ=255)
                      aCGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCgg                                                                          <  1:1863896/100‑1 (MQ=255)
                       cGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGc                                                                         >  2:1886081/1‑100 (MQ=255)
                       cGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGc                                                                         <  1:2375623/100‑1 (MQ=255)
                        ggCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGcc                                                                        <  2:20676/100‑1 (MQ=255)
                        ggCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGcc                                                                        <  1:592808/100‑1 (MQ=255)
                         gCCCCGGTACGGTGTACAGTTTCGCCAGTAGATTCAGCGGGGCATAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGccc                                                                       <  1:2428656/100‑1 (MQ=255)
                          ccccGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGcccc                                                                      >  2:2226534/1‑100 (MQ=255)
                          ccccGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGcccc                                                                      >  2:16698/1‑100 (MQ=255)
                          ccccGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGcccc                                                                      >  1:2059714/1‑100 (MQ=255)
                           cccGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCg                                                                     <  2:1473783/100‑1 (MQ=255)
                           cccGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCg                                                                     >  1:1867011/1‑100 (MQ=255)
                           cccGGCACGGTGTACTGTTACGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCg                                                                     <  1:873664/100‑1 (MQ=255)
                                 aCGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTc                                                               >  1:1016097/1‑100 (MQ=255)
                                   ggTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGc                                                             <  1:971096/100‑1 (MQ=255)
                                   ggTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGc                                                             >  1:1784953/1‑100 (MQ=255)
                                   ggTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGc                                                             <  1:1614112/100‑1 (MQ=255)
                                     tgtACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCAt                                                           <  1:642704/100‑1 (MQ=255)
                                      gtACTGTTTCGCCCGTAGATTCAGCGGACCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATg                                                          >  1:2324639/1‑100 (MQ=255)
                                      gtACTGTTTCGCCAGTAGCTTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATCCGGCCCCGTCCCCCCCATg                                                          >  2:2314372/1‑100 (MQ=255)
                                      gtACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATg                                                          >  2:209390/1‑100 (MQ=255)
                                       tACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGa                                                         <  2:1928093/100‑1 (MQ=255)
                                         cTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGaaa                                                       >  2:174311/1‑100 (MQ=255)
                                          tGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAAt                                                      >  1:1830380/1‑100 (MQ=255)
                                                gCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGtt                                                >  1:1797684/1‑100 (MQ=255)
                                                    gTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAg                                            <  1:2623388/100‑1 (MQ=255)
                                                    gTAGATTCAGCGGGACAAAATGAACATAGTTCGCCAGCTCCTGACACACGCGTAACAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAg                                            >  2:697960/1‑100 (MQ=255)
                                                       gATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTtg                                         >  2:2273751/1‑100 (MQ=255)
                                                       gATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTtg                                         >  2:1774514/1‑100 (MQ=255)
                                                            aGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTgcg                                    <  1:531499/100‑1 (MQ=255)
                                                            aGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTgcg                                    >  1:891961/1‑100 (MQ=255)
                                                            aGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTgcg                                    <  2:790173/100‑1 (MQ=255)
                                                             gCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTgcgg                                   >  1:1513898/1‑100 (MQ=255)
                                                              cGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTgcggc                                  <  2:616126/100‑1 (MQ=255)
                                                               ggggCTAAATCAACATAGTTCGCCAACCCCTGACACACGCGTACCAGCACTTCATGCGTCCCCCTCACTCGCTTGAAATACCGTTGCAGTTGCTgcggcg                                 >  2:106809/1‑100 (MQ=255)
                                                               ggggCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTgcggcg                                 <  1:2183668/100‑1 (MQ=255)
                                                               ggggCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTgcggcg                                 <  1:1401063/100‑1 (MQ=255)
                                                                 ggCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTc                               >  2:1945678/1‑100 (MQ=255)
                                                                 ggCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTc                               >  2:694010/1‑100 (MQ=255)
                                                                 ggCAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTc                               >  1:1288215/1‑100 (MQ=255)
                                                                   cAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAg                             >  1:1497794/1‑100 (MQ=255)
                                                                   cAAAATCAACATAGTTCGCCAGCTCCTGACACACGCGCACCAGCCCCTCATGCGGCCCCGTCCCTTGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAg                             <  1:2456006/100‑1 (MQ=255)
                                                                    aaaaTCAACATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAgg                            >  1:600495/1‑100 (MQ=255)
                                                                           aCATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAGGTTTTTCa                     <  1:839204/100‑1 (MQ=255)
                                                                           aCATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAGGTTTTTCa                     <  2:686971/100‑1 (MQ=255)
                                                                            cATAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAGGTTTTTCaa                    >  2:1240792/1‑100 (MQ=255)
                                                                             aTAGTTCGCCAGCTCCTGACACACTCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAGGTTTTTCAAc                   <  1:1202680/100‑1 (MQ=255)
                                                                             aTAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAGGTTTTTCaan                   <  1:2097850/100‑2 (MQ=255)
                                                                             aTAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAGGTTTTTCAAc                   >  1:1971165/1‑100 (MQ=255)
                                                                             aTAGTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAGGTTTTTCAAc                   <  2:1170163/100‑1 (MQ=255)
                                                                              tAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAGGTTTTTCAAct                  <  1:390915/100‑1 (MQ=255)
                                                                              tAGTTCGCCAGCTCCTGACACACGCGAACCAGCCCTTCATGCGGCCCCGCCCCTCGCATGAACTACCGTTGCAGTTGCTCCGGCGTCAGGTTTTTCAAct                  >  2:309923/1‑100 (MQ=255)
                                                                                gTTCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAGGTTTTTCAActct                >  2:668086/1‑100 (MQ=255)
                                                                                 ttCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCtt               <  1:1925059/100‑1 (MQ=255)
                                                                                  tCGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTc              >  1:1582488/1‑100 (MQ=255)
                                                                                   cGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCa             >  2:2595292/1‑100 (MQ=255)
                                                                                   cGCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCa             >  1:1318716/1‑100 (MQ=255)
                                                                                    gCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCAt            >  1:372290/1‑100 (MQ=255)
                                                                                    gCCAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCAt            <  1:1661017/100‑1 (MQ=255)
                                                                                     ccAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATg           >  2:306520/1‑100 (MQ=255)
                                                                                     ccAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATg           >  1:664549/1‑100 (MQ=255)
                                                                                     ccAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATg           >  2:1992479/1‑100 (MQ=255)
                                                                                     ccAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTACTGCGGCGTCAGGTTTTTCAACTCTTCATg           >  2:1132070/1‑100 (MQ=255)
                                                                                      cAGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTCCTGCGGCGTCAGGTTTTTCAACTCTTCATgg          <  2:1069847/100‑1 (MQ=255)
                                                                                       aGCTCCTGACACACGCGTTCCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGc         <  1:2478428/100‑1 (MQ=255)
                                                                                       aGCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGc         <  1:2569038/100‑1 (MQ=255)
                                                                                        gCTCCTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATCCCGTTGCAGTTGCTGCGGCGTCAGGGCTTTCAACTCTTCATGGCg        >  2:1602474/1‑100 (MQ=255)
                                                                                           ccTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCgata     <  1:2013320/100‑1 (MQ=255)
                                                                                           ccTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCgata     <  1:1040510/100‑1 (MQ=255)
                                                                                            cTGACACGCGGGTACCAGACCTTCAGGCGGCCCTGTCCCTCGCATGAAATCCCGTTGCAGTTGCTGAGGCGTCAGGTTTTTCCACTCTTCATGGCgatag    <  2:247802/100‑1 (MQ=255)
                                                                                            cTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCgatag    <  1:1878268/100‑1 (MQ=255)
                                                                                            cTGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCgatag    <  1:1765963/100‑1 (MQ=255)
                                                                                             tGACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCgataga   <  1:1701574/100‑1 (MQ=255)
                                                                                              gACACACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCgatagat  >  1:1264500/1‑100 (MQ=255)
                                                                                                 |                                                                                                
TTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAAATACCGTTGCAGTTGCTGCGGCGTCAGGTTTTTCAACTCTTCATGGCGATAGAT  >  NC_000913/1968673‑1968866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A