Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F412 I2 R1
|
70 |
105.0 |
5244496 |
94.3% |
4945559 |
100.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,346,140 |
T→C |
F426L (TTT→CTT) |
nrdA → |
ribonucleoside‑diphosphate reductase 1, alpha subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,346,140 | 0 | T | C | 98.6%
| 253.6
/ NA
| 72 | F426L (TTT→CTT) | nrdA | ribonucleoside‑diphosphate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (37/34); minor base A (1/0); total (38/34) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GAAAGCCGTTGAGCTGTTCTCGCTGATGATGCAGGAACGTGCGTCTACCGGTCGTATCTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGTTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGCTGAACGACGTCAACGACGAGAACGGTG > NC_000913/2346043‑2346236
|
gAAAGCCGTTGAGCTGTTCTCGCTGATGATGCAGGAACGTGCGTCTACCGGTCGTATCTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGctt > 2:653240/1‑100 (MQ=255)
gAAAGCCGTTGAGCTGTTCTCGCTGATGATGCAGGAACGTGCGTCTACCGGTCGTATCTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGctt < 1:557202/100‑1 (MQ=255)
ttGAGCTGTTCTCGCTGATGATGCAGGAACGTGCGTCTACCGGTCGTATCTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGc < 2:2191006/100‑1 (MQ=255)
ttGAGCTGTTCTCGCTGATGATGCAGGAACGTGCGTCTACCGGTCGTATCTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGc < 2:2190393/100‑1 (MQ=255)
ttGAGCTGTTCTCGCTGATGATGCAGGAACGTGCGTCTACCGGTCGTATCTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGc < 2:150645/100‑1 (MQ=255)
ttGAGCTGTTCTCGCTGATGATGCAGGAACGTGCGTCTACCGGTCGTATCTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGc < 1:147586/100‑1 (MQ=255)
aGCTGTTCTCGCTGATGATGCAGGAACGTGCGTCTACCGGTCGTATCTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCAt > 2:1365823/1‑100 (MQ=255)
aGCTGTTCTCGCTGATGATGCAGGAACGTGCGTCTACCGGTCGTATCTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCAt > 2:1202142/1‑100 (MQ=255)
cTGTTCTCGCTGATGATGCAGGAACGTGCGTCTACCGGTCGTATCTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATcg < 2:2563141/100‑1 (MQ=255)
tctcGCTGATGATGCAGGAACGTGCGTCTACCGGTCGTATCTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGcc < 1:1175544/100‑1 (MQ=255)
tctcGCTGATGATGCAGGAACGTGCGTCTACCGGTCGTATCTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGcc > 2:997888/1‑100 (MQ=255)
tcGCTGATGATGCAGGAACGTGCGTCTACCGGTCGTATCTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAg > 2:812072/1‑100 (MQ=255)
cggttgatgCAGGAACGTGCTGCTACCTGTCGGATCCGGATTCAGAACGGTGACGAGTGCAATACCCACGGGCCGCTGGATCCGGCCATCGCGCCAGTGc < 2:516496/96‑1 (MQ=255)
gatgatGCAGGAACGTGCGTCTACCGGTCGTATCTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGt < 2:263917/100‑1 (MQ=255)
atgatgCAGGAACGTGCGTCTACCGGTCGTATCTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTc < 1:825512/100‑1 (MQ=255)
gatgCAGGAACGTGCGTCTACCGGTCGTATCTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAg < 1:823727/100‑1 (MQ=255)
gatgCAGGAACGTGCGTCTACCGGTCGTATCTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAg > 1:1233706/1‑100 (MQ=255)
gatgCAGGAACGTGCGTCTACCGGTCGTATCTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAg < 2:2522100/100‑1 (MQ=255)
tgCAGGAACGTGCGTCTACCGGTCGTATCTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTc > 1:1931652/1‑100 (MQ=255)
gCAGGAACGTGCGTCTACCGGTCGTATCTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCt > 1:34251/1‑100 (MQ=255)
cAGGAACGTGCGTCTACCGGTCGTATCTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTa > 1:2569947/1‑100 (MQ=255)
cAGGAACGTGCGTCTACCGGTCGTATCTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTa > 1:468668/1‑100 (MQ=255)
cAGGAACGTGCGTCTACCGGTCGTATCTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTa < 1:501697/100‑1 (MQ=255)
gAACGTGCGTCTACCGGTCGTATCTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGCGTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAAcc < 2:1825273/100‑1 (MQ=255)
aCGTGCGTCTACCGGTCGTATCTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCtg > 2:936435/1‑100 (MQ=255)
cGTGCGTCTACCGGTCGTATCTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCtgt > 1:2075002/1‑100 (MQ=255)
cGTGCGTCTACCGGTCGTATCTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCtgt > 1:882425/1‑100 (MQ=255)
gTGCGTCTACCGGTCGTATCTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTGGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCtgtg > 1:2525531/1‑100 (MQ=255)
tGCGTCTACCGGTCGTATCTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGc > 1:431814/1‑100 (MQ=255)
gCGTCTACCGGTCGTATCTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGcc < 1:528567/100‑1 (MQ=255)
gTCTACCGGTCGTATCTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTg > 1:1312378/1‑100 (MQ=255)
tACCGGTCGTATCTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGgag > 2:1982712/1‑100 (MQ=255)
tACCGGTCGTATCTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGgag > 2:1982711/1‑100 (MQ=255)
cGGTCGTATCTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATa > 2:674242/1‑100 (MQ=255)
tATCTATATTCAGAACGTTGACCACTGCAATACCCATGGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTg < 1:755632/100‑1 (MQ=255)
tccatatTCAGAATTTTGACCACTGCAATACCCACAGCCCGCGTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCGTGGAGATAGCCCGGcc < 2:916223/97‑1 (MQ=255)
tCTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGCGATAGCCCTGcc > 1:1482379/1‑100 (MQ=255)
tCTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGcc > 2:473029/1‑100 (MQ=255)
cTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCg < 2:351108/100‑1 (MQ=255)
tataTTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGa > 1:2223619/1‑100 (MQ=255)
tataTTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGa > 1:1245624/1‑100 (MQ=255)
atatTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGAc > 2:574137/1‑100 (MQ=255)
atTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCa < 1:1902755/100‑1 (MQ=255)
atTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCa > 1:2476511/1‑100 (MQ=255)
atTCAGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCa > 1:2506533/1‑100 (MQ=255)
ttCAGAACGTTGACCACTGCAATACCCATACCCCGCTTGATCCGGCCACCGCGCCAGTGCGTCAGTCTAACCTGTGTCTGGAGATAGCCCTGCCGACCaa < 1:2518166/100‑1 (MQ=255)
aGAACGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAAcc > 2:2517604/1‑100 (MQ=255)
aaCGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGc < 2:867472/100‑1 (MQ=255)
aaCGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGc < 1:1254591/100‑1 (MQ=255)
aCGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGCt < 1:1176469/100‑1 (MQ=255)
aCGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGCt < 1:1177023/100‑1 (MQ=255)
cGTTGTCCACTGCAATACCCATAGCCCGCTTGATCCGGCCAGCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGCTg < 2:1662822/100‑1 (MQ=255)
cGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGCTg < 2:8512/100‑1 (MQ=255)
cGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGCTg < 2:465115/100‑1 (MQ=255)
cGTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGCTg < 1:9721/100‑1 (MQ=255)
gTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCGCTGCCGACCCAACCGCTGa > 2:1867213/1‑100 (MQ=255)
gTTGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGCTGa > 1:50005/1‑100 (MQ=255)
ttGACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGCTGaa < 1:112749/100‑1 (MQ=255)
gACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGCTGAacg > 2:2153585/1‑100 (MQ=255)
gACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGCTGAacg > 2:532333/1‑100 (MQ=255)
gACCACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGCTGAacg < 1:2258062/100‑1 (MQ=255)
ccACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGCTGAacgac > 2:2018695/1‑100 (MQ=255)
cACTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGCTGAacgacn < 1:2094404/100‑2 (MQ=255)
aCTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGCTGAACGACGt < 1:1376163/100‑1 (MQ=255)
cTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGCTGAACGACGTc < 1:266609/100‑1 (MQ=255)
cTGCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGCTGAACGACGTc < 2:881392/100‑1 (MQ=255)
gCAATACCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGCTGAACGACGTCaa > 1:1634620/1‑100 (MQ=255)
tACCCATAGCCCGCTTGATCCGGCCATCGCGCCAATGCGTCAGTCTAACCTGTACCTGGAGATAGCCCTGCCGAACAAACCGCTGAACGACGTCAacgac > 1:2453252/1‑100 (MQ=255)
aCCCATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGCTGAACGACGTCAacgacg > 2:1536384/1‑100 (MQ=255)
cccATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTCACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGCTGAACGACGTCAAcgacga > 2:1508474/1‑100 (MQ=255)
cccATAGCCCGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGCTGAACGACGTCAAcgacga > 1:2440909/1‑100 (MQ=255)
cccGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGCTGAACGACGTCAACGACGAGAACGGt > 1:777201/1‑100 (MQ=255)
ccGCTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGCTGAACGACGTCAACGACGAGAACGGTg < 2:521534/100‑1 (MQ=255)
ccGATTGATCCGGCCATCGCGCCAGTGCGTCCGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGCTGAACGCCGTCAACGACGAGAACGGTg > 2:2383297/1‑100 (MQ=255)
|
GAAAGCCGTTGAGCTGTTCTCGCTGATGATGCAGGAACGTGCGTCTACCGGTCGTATCTATATTCAGAACGTTGACCACTGCAATACCCATAGCCCGTTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGCTGAACGACGTCAACGACGAGAACGGTG > NC_000913/2346043‑2346236
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
