Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F403 I0 R1	51	156.8	4085907	97.3%	3975587	179.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,151,689	G→T	35.9%	intergenic (+43/+22)	yegJ → / ← yegK	uncharacterized protein/ser/thr phosphatase‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,151,689	0	G	T	35.9%	13.0 / 10.3	40	intergenic (+43/+22)	yegJ/yegK	uncharacterized protein/ser/thr phosphatase‑related protein
Reads supporting (aligned to +/- strand): ref base G (18/6); new base T (9/5); total (27/13)
Fisher's exact test for biased strand distribution p-value = 7.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.64e-01

AATCAAGAAA‑GAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTAC‑GGCGGTTATACCATTGATCCTTT‑ATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCG‑GT‑TG‑AA‑TTGTTG‑TCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATC‑GA‑GTTGTTCCTGCGTTGCTGCCGCCAGT‑CCA‑TT‑GAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCAT  >  NC_000913/2151511‑2151916

aactaagaaaggAAGATGTTACAGTCGGGATGTATGCGCGGGGGAATAAAATTGAG‑GTGTGTAATA‑CATTTGTCCTTTAATTGCCCACCTAACAAAAAAAAAACCCCGCAGAGTTAAAAAAAAATTTGTTTCGCAAACCGGCCTTTATTTTTTTTTTTAAAA‑CGGGGG‑TT‑TA‑AT‑TTTTTG‑TCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCa                                                                                                                                                                                           <  2:1304544/223‑1 (MQ=255)

                        gcgtggtatataCGCGCGGGTAAAAAAATTACGGGCGGTTATACATTTCATCTTTT‑TTTGGCCACCAAAACAAAAAAAAAAGCCGACGATAAAAAAAAAAATTTAGTCCCTAAACCACCCTTTATTTTTATTTTTAAAC‑CGGGCG‑GT‑TA‑AT‑TTTTTG‑TCTTAAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGtt                                                                                                                                                                                      <  2:142996/201‑1 (MQ=255)

                                                                        gATCCTTT‑ATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCG‑GT‑TG‑AA‑TTTTTT‑TCTTTAACCGCCCTGAGAAACTCAATCTTCCCCCAACGCCCGTGCCAACGGTTTATCATCATcccttc                                                                                                                                                                      >  2:178162/1‑173 (MQ=255)

                                                                        gATCCTTT‑ATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCG‑GT‑TG‑AA‑TTTTTT‑TCTTCAACCGCCCTGTGAAAACCACCCTCCCCACAGCGCCAGGGGCCACGTTTTATCACTATTCCTAACCCCGCTTAACCCCCGGCTGGGGAAAAAATGTCTTAACAATTTCGGtatga                                                                                                                   >  2:712428/1‑225 (MQ=255)

                                                                         aTCCTTT‑ATTGGTCCCCTATCAAAAAGAAGAAGCCGACGAGATAAGAGCAAAATTAGTTCCTTAATCAGCCTTTATATTTTTTTTTTAAA‑CGGGGG‑TT‑TA‑AT‑TTTTTT‑TCTTAAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACg                                                                                                                                                                     <  1:178468/177‑1 (MQ=255)

                                                                                    ggTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCG‑GT‑TG‑AA‑TTGTTG‑TCTTCAACCGCCCTGAGAAACTCACTCTGCCCCCAGCGCCAGTGCCAACGTTTTTTCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATC‑GA‑gttgt                                                                                                      >  1:812693/1‑228 (MQ=255)

                                                                                     gTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCG‑GT‑TG‑AA‑TTTTTG‑TTTTCAACCGCCCTTAGAAAATCACCCTTCCCCCAACCCCAGGGCCAACGTTTTTTCATAATCCCTACCCCCGTTTTCCGCCCGGCCGGCCCAAAACTGCTTTTATAAATCAGGTAATAAATC‑GA‑gtt                                                                                                        >  2:251585/1‑225 (MQ=255)

                                                                                      tCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCG‑GT‑TG‑AA‑TTGTTG‑TCTTTCACCGCCCTGAGAAACTCACTCTTCCCCCAGCGCCCGTGCCCAAGTTTTTTTCTCATCCGTACGCCCGGTTTCCGCCGGGGTGGGCAAAAAATTGTTTTACAATTCACGTCATAAATC‑gcggtttt                                                                                                      >  2:1755559/1‑220 (MQ=255)

                                                                                            aTCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCG‑GT‑TG‑GA‑TTGTTT‑TTTTCCACCGCCCTGAGAAACTCACTCTGCCCACAACGCCAGTGCCCACGTTTTATCATCATCCGCAAGCTCCTTTACCGCCCGGCTGGACCAAAACTGCTTTTACCAATCAAGGAATTAATC‑GA‑GTTGTTCCTGccg                                                                                              >  2:909302/1‑226 (MQ=255)

                                                                                             tCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCG‑GT‑TG‑AA‑TTTTTT‑TCTTCAACCCCCATGAGGAACTCACCCTGCCCACAACGCCCGTGGCAACGTTTTATCATCATCCGTACGCCCGGTTTTCGCCGGGCTGGGCAAAAAATGCCTTAAAAATTCCAGGAATAAATC‑GA‑GGTGTTTCTTCgg                                                                                              >  2:994434/1‑226 (MQ=255)

                                                                                             tCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCG‑GT‑TG‑AA‑TTGTTG‑TCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCCCGGTTACCGCCggg                                                                                                                                                     >  1:698200/1‑174 (MQ=255)

                                                                                                       gaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCG‑GT‑TG‑AA‑TTTTTG‑TCTTCAACCGc                                                                                                                                                                                                                               >  1:543144/1‑90 (MQ=255)

                                                                                                       gaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCG‑GT‑TG‑AA‑TTGTTG‑TCTTCAACCGCCATGAGAAAATCACTCTTCCCCCAAGGCCAGGGCCAACCTTTTATCATCATCCGTACGCCCGGTTACCGCCCGGCTGGGTAAAAAAAGGTTTTAACATTTAGGGAATTAATCAGA‑G‑TGTGTCTGCGTTGCAgccgccc                                                                                   >  2:922614/1‑227 (MQ=255)

                                                                                                       gaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCG‑GT‑TG‑AA‑TTGTTG‑TCTTCAACCGCCATGAGAAAATCACTCTGCCCCCAGCGCCAGTGCCAACGGTTTATCATCATCCGTACGCCCGTTTTCCCCCGGGGCGGGCAAAAAATGCTTTTACAATTCAGGGGATAAAAT‑CA‑GTTGTTTCTTCGGTGCTtccgccc                                                                                   >  2:300348/1‑227 (MQ=255)

                                                                                                        aagCCGAGGAGCAAAGACAAATATTAGTTCTCTATCCAGCTTTTATATTTTTTTTTTAAG‑CTGGGG‑GT‑TG‑AT‑TTTTTT‑TCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTc                                                                                                                           <  1:1323231/189‑1 (MQ=255)

                                                                                                                           aaaaTTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCG‑GT‑TG‑AA‑TTGTTG‑TTTTCAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGGCAAAAACTGCTTTTACAATTCAGGTTATAAAAC‑GA‑GATGTTCATGCGTTTCTGCCGGCCGT‑GCC‑TT‑Gaaaaaaaggggt                                                            >  2:1341281/1‑221 (MQ=255)

                                                                                                                            aaaTTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCG‑GT‑TG‑AA‑TTGTTT‑TTTTCAACCGCCATTAGAAACTCACTCTTCCCACAGCGCCCGTGGCAACGTTTTTTCATCATCCCTACGCCCCTTTACCCCCCGGCGGGGCAAAAAATTCTTTAAAAATTCAGGGAATAAATC‑GATGCGGTTCCTCCTTTCTTGCGGCC‑GT‑CCACTT‑GAAAAATgggg                                                              >  2:1487159/1‑226 (MQ=255)

                                                                                                                            aaaTTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCG‑GG‑TT‑AA‑TTTTTG‑TCTTCCACCGCCATTAGAAACTCACTCTACCCCCAGCGCCCGTGCCAACGGTTTTTCATCATCCGTACGCCCGTTTTCCGCCCGGGTGGACAAAAACAGCTTTAACAAATCTGGGAATAAATT‑GA‑AGTGTTTCCGCTGTGGTTTCGCCCGT‑TCA‑TT‑GGAAAACcgggga                                                            >  2:1619972/1‑225 (MQ=255)

                                                                                                                             aaTTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCG‑GT‑TG‑AA‑TTGTTG‑TCTTCAAACGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCCCGTTTACCGCCCGGCTGGACAAAAACTGCTTTAACAAATCAGGTAATAAATC‑GA‑GTTGTTCCTGCGTTGCTTCCGCCAGT‑CCA‑TT‑GGAAAACAGGGt                                                             >  2:624734/1‑225 (MQ=255)

                                                                                                                                  gTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCG‑GT‑TG‑AA‑TTGTTG‑TCTTTAAACCCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCCAAGGTTTATCATCATCCGTAAGCCCGTTTACCGCCGGGCCGGGCCAAAAATGCTTTTACAATTCAGGt                                                                                                                       >  2:566090/1‑167 (MQ=255)

                                                                                                                                   ttCTCTAACCACCCTTTATTTTTTTTTTTAAAC‑CGGGGG‑GT‑TA‑AA‑TTGTTG‑TCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGta                                                                                                                      <  1:567012/167‑1 (MQ=255)

                                                                                                                                   ttCGCTAATCCCCCTTTATTTTTTTTTTTAAAC‑CTGGGG‑GT‑TA‑AA‑TTTTTT‑TCTTCAACCGCCATGAGAAactcact                                                                                                                                                                                                                <  1:11147/77‑1 (MQ=255)

                                                                                                                                   ttCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCG‑GT‑TT‑AA‑TTGTTG‑TCTTCAACCGCCCTTAGAAACTTACTCTTCCCCCCGCGCCAGTGCCAACGGTTTATCATCATCCGTACGCTCGTTTTCCGCCGGGCTGGGCAAAAACTGCTTTTACAATTCAGGGAATTAAAC‑GA‑GGTGTTTCTGCGGTTCTTCCCCCAGT‑CCA‑TT‑GGAAAACAGGGGAAAGAAAgg                                                    >  2:1710121/1‑228 (MQ=255)

                                                                                                                                       gataaccggctttATTTTTTTTTTTTAAACCGGGGG‑GT‑TA‑AT‑TTGTTT‑TCTAAACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATC‑GA‑GTTGTTCCTGCGTTGCTGCCGCCAGT‑CCA‑TT‑GAAAAACGGGGTAAAGAAAGGcac                                                 <  1:1343686/217‑1 (MQ=255)

                                                                                                                                         gaTCAGCC‑TTTTATTTTTTTTTTAAA‑CCGGGGCGT‑TT‑AATTTGTTT‑CCCACACCCGCCATGAGAAACTCCCTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATC‑GA‑GTTGTTCCTGCGTTGCTGCCGCCAGT‑CCA‑TT‑GAAAAACGGGGTAAAGAAAGGCACAt                                               <  2:811298/227‑1 (MQ=255)

                                                                                                                                            tcaagtttATTTTTTTTGTATAAG‑CGGGGG‑GT‑TA‑AA‑TTTTTC‑TCTTCAACCGCCATGAGAAACTCCCTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATC‑GA‑GTTGTTCCTGCGTTGCTGCCGCCAGT‑CCA‑TT‑GAAAAACGGGGTAAAGAAAGGCACATgag                                            <  1:234680/221‑1 (MQ=255)

                                                                                                                                            gccgccttAAATTTATGTGTTAAA‑GCGGGG‑GT‑TGAAA‑TTTTTC‑TCTACAACCGCCATGAGAAACTCCCTCTGCCCACAGCGCCAGGGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATC‑GA‑GTTGTTCCTGCGTTGCTGCCGCCAGT‑CCA‑TT‑GAAAAACGGGGTAAAGAAAGGCACATgag                                            <  1:1489817/222‑1 (MQ=255)

                                                                                                                                             aGCCGTTATATTTATTGTTTAAA‑CTGGCG‑GT‑TG‑AA‑TTGTTG‑TCTTCAACCGCCATTAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGGACGCTCGTTTACCGCCGGGGTGGGCAAAAAATGCTTTAACAATTc                                                                                                                           >  1:1175442/1‑152 (MQ=255)

                                                                                                                                              gCCTTTATTTTTTTTTTTAAAA‑CTGGCG‑TT‑TA‑AA‑TTTTTG‑TCTTCACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCa                                                                                                                          <  2:1173337/152‑1 (MQ=255)

                                                                                                                                                  ttATATTTATTGTTTAAA‑CTGGCG‑GT‑TG‑AA‑TTTTTT‑TCTTCAAACGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGGTTTATCATCATCCGTACGCTCGTTTACCGCCCGGCTGGGCAAAAAATGCTTTAACAATTTAGGTAATAAATC‑GA‑GTTGTTTCTGCGTTGCTGTCGCCAGG‑CCC‑TT‑GAAAAAACGGGTTAAGAAAGGCACATGAGGAGaaat                                     >  2:664043/1‑226 (MQ=255)

                                                                                                                                                  ttATATTTATTGTTTAAA‑CTGGCG‑GT‑TG‑AA‑TTGTTG‑TCTTCAACCGCCATGAGAAACTCACTCTGCCCACAACGCCAGTGCCAAAGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAAAAAtt                                                                                                                            >  1:205367/1‑146 (MQ=255)

                                                                                                                                                            tttttAAA‑CCGGGG‑GTATA‑AT‑TTGTTG‑TCTAAAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTCCGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATC‑GA‑GTTGTTCCTGCGTTGCTGCCGCCAGT‑CCA‑TT‑GAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGc                              <  1:1758661/224‑1 (MQ=255)

                                                                                                                                                            tGTTTAAA‑CTGGCG‑GT‑TG‑AA‑TTTTTG‑TCTTTAACCGCCCTGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGGTTTTTCATCATCCGTACGCTCCTTTACCCCCGGGCTGGACAAAAAATGCTTTAACAATTCAGGGAATAAAAC‑GA‑GGTGTTCCTGCGGTGGTTCCGCCAGT‑CCA‑TT‑GGAAAACCGGGTAAAGAAAGGGACATGAGGAGAATTAACCAGcctc                           >  2:1684113/1‑225 (MQ=255)

                                                                                                                                                            tGTTTAAA‑CTGGCG‑GT‑TG‑AA‑TTGTTT‑TTTTCAACCGCCATGAGAAACTCACTCTGCCCCCAGCGCCCGTGCCCACGTTTTTTTATCATCCGTACGCTCGTTTTCCGCCCGGGTGGGCCAAAAATGCTTTTACAATTTCGGGAATAAAAC‑CG‑GGTGTTCCCTCGTTTCTTCCGCCCGG‑CCA‑TT‑TAAAAAAGGGGGAAAGAAAGGCACATTAGGGGAAATTTcccatg                             >  2:234284/1‑222 (MQ=255)

                                                                                                                                                            tGTTTAAA‑CTGGCG‑GG‑TG‑AA‑TTGTTG‑TCTTCAACCGCCCTGAGAAAATCACTCTTCCCCCAGCGCCAGTGGCAAAGGTTTTTCATCATACCTAAGCTCGTTTTACGCCGGGCTGGACAAAAACTGCTTTAAAAATTCAGGGAATAAAAC‑GA‑GTTGTTTCTGCGTTGCTGCCGCCAGT‑CCA‑TTGGAAAAACGGGG‑AAAGAAAAGAGCATGAGGAGAACTATCACGCata                           >  2:1808128/1‑228 (MQ=255)

                                                                                                                                                               ttAAA‑CTGGCG‑GT‑TG‑AA‑TTGTTT‑TCTTCAACCCCCATGAGAAAATCACTCTTCCCACAACGCCAGTGCCCACGTTTTATCATCATCCCTACGCCCGTTTACCGCCGGGCCGGACAAAAACTGCTTTTACAATTCCGGTAATAAATT‑GG‑GGTGTTTCCGCGTTGTTGCCCCCCGTCCCT‑TT‑TAAAAAAGGGGTAAGAAAAGCGACATAGGGGGATATAT‑CAGCATAtt                         >  2:1840091/1‑227 (MQ=255)

                                                                                                                                                               ttAAA‑CTGGCG‑GT‑TG‑AA‑TTGTTG‑TCTTCAAACGCCATGAGAAAATCACTCTGCCCACAGGGGCAGTGcc                                                                                                                                                                                            >  1:459908/1‑69 (MQ=255)

                                                                                                                                                                tAAA‑CTGGCG‑GT‑TG‑AA‑TTGTTG‑TCTTCAACCGCCCTGAGAAACTCACTCTTCCCACAACGCCAGTGCCAACGTTTTATCATCATCCGTAAGCCCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATC‑GA‑GTTGTTCCTGCGTTGCTGCCGCCAGT‑CCC‑TT‑TAAAAAAGGGGTAAAGAAAGGCACATCAGGGGAATTTTCCAGCCAATTTa                       >  2:154254/1‑228 (MQ=255)

                                                                                                                                                                         cG‑GT‑TG‑AA‑TTGTTT‑TCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCCGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATC‑GC‑GTTGTTCCTTCGTTGCTGCCGCCAGT‑CCA‑TT‑GAAAAAAGGGGTAAAGAAAAGACCATGAGGGGAATTATTCAGCATAATTactgttctc               >  2:561032/1‑220 (MQ=255)

                                                                                                                                                                            gT‑TT‑AA‑TTTTTGCTCTCCACCCGCCATGAGAACCTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATC‑GA‑GTTGTTCCTGCGTTGCTGCCGCCAGT‑CCA‑TT‑GAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATc                                  <  1:561947/208‑1 (MQ=255)

                                                                                                                                                                                          g‑tCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATC‑GA‑GTTGTTCCTGCGTTGCTGCCGCCAGT‑CCA‑TT‑GAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATAATTAACGCCAGTCGCTGGATACCAt  >  1:892131/1‑228 (MQ=255)

AATCAAGAAA‑GAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTAC‑GGCGGTTATACCATTGATCCTTT‑ATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCG‑GT‑TG‑AA‑TTGTTG‑TCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATC‑GA‑GTTGTTCCTGCGTTGCTGCCGCCAGT‑CCA‑TT‑GAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCAT  >  NC_000913/2151511‑2151916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 6 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A