Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I51 R1 223 69.9 1534784 95.1% 1459579 214.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 924,571 T→G I436M (ATT→ATG)  clpA → ATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913924,5710TG97.6% 120.7 / ‑3.1 41I436M (ATT→ATG) clpAATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (19/21);  total (19/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.25e-01

AACCGAAGTATGAAGCGCACCACGACGTGCGTTATACCGCAAAAGCGGTGCGTGCGGCGGTAGAGCTGGCGGTGAAATACATTAACGATCGTCATCTGCCGGATAAAGCCATTGATGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGA‑GTCCGTGGTGGCCCGTATTGCACGCATTCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTG‑AAAATGCTGGTCTTCGGTCAGGATAAAG‑CCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGGTTCGTTCCTGTTTGCCGGCCCTACCGGGGTCGGGAAAACAGAGGTGACGGTACAGCTTTCGAAAGCTTTGGG  >  NC_000913/924348‑924801
                                                                                                                                                                                                                                |                                                                                                                                                                                                                                        
aaCCGAAGTATGAAGCGCCCCACGACGTGCGTTATACCGCAAAAGCGGTGCGTGCGGCGGTAGAGCTGGCGGTGAAATACATTAACGATCGTCATCTGCCGGATAAAGCCATTGATGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGA‑GTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAagag                                                                                                                                                                                                                               <  1:204115/235‑1 (MQ=255)
aaCCGAAGTATGAAGCGCACCACGACGTGCGTTATACCGCAAAAGCGGTGCGTGCGGCGGTAGAGCTGGCGGTGAAATACATTAACGATCGTCATCTGCCGGATAAAGCCATTGATGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGA‑GTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAgt                                                                                                                                                                                                                              <  2:3062/236‑1 (MQ=255)
   cGAAGTATGAAGCGCACCACGACGTGCGTTATACCGCAAAAGCGGTGCGTGCGGCGGTAGAGCTGGCGGTGAAATACATTAACGATCGTCATCTGCCGGATAAAGCCATTGATGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGA‑GTCCGTGGTGGCCCGTATTGCACGCATGCCAGagaaga                                                                                                                                                                                                                                <  1:470319/231‑1 (MQ=255)
                     acgacgTGCGTTATACCGCAAAAGCGGTTCGTGCGGCGGTAGAGCTGGCGGTGAAATACATTAACGATCGTCATCTGCCGGATAAAGCCATTGATGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGA‑GTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGa                                                                                                                                                                                                          <  1:175739/235‑1 (MQ=255)
                            gCGTTATACCGCAAAAGCGGTGCGTGCGGCGGTAGAGCTGGCGGTGAAATACATTAACGATCGTCATCTGCCGGATAAAGCCATTGATGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGA‑GTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTg                                                                                                                                                                                                           <  1:128233/227‑1 (MQ=255)
                                                 gcctgcgGCGGTAGAGCTGGCGGTGAAATACATTAACGGTCGTCATCTGCCGGATAAAGCCATTGATGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGA‑GTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCtt                                                                                                                                                                               <  1:664042/231‑1 (MQ=255)
                                                         cggTAAGGCTGGCGGTGAAATACATTAACGATCGTCATCTGCCGGATAAAGCCATTGATGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGA‑GTCCGTGGTGGCCCGTATTGCACGCATTCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTG‑AAAATGCt                                                                                                                                                                     <  1:334199/235‑1 (MQ=255)
                                                                   ggCGGTGAAATACATTAACGATCGTCATCTGCCGGATAAAGCCATTGATGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGA‑GTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCt                                                                                                                                                                                <  1:14279/215‑1 (MQ=255)
                                                                     cGGTGAAATACATTAACGATCGTCATCTGCCGGATAAAGCCATTGATGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGGAATTGAGGTCCG‑GGGGGGCCGGATTTCACGCAAGCCAGAGAAGAGTGTTTCTCACAGAGGTCGTGGTAACCTGGAAAACCCCTGCGATAGCGTGTAAAATCCTgtctttgggccg                                                                                                                                                         >  2:27180/1‑225 (MQ=255)
                                                                              aCATTAACGATCGTCATCTGCCGGATAAAGCCATTGATGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGA‑GTCCGGGGGGGCCCGGATTGCACGCATGCCCGAGAAGAGTGTTTCTCAGAGTGAGCGGGATACCCTGAAAAACCTCTGCGATCGCTTG‑TAAATGCTGGGCTTCGTGCAGGGTTAAgac                                                                                                                                               >  2:395790/1‑234 (MQ=255)
                                                                                aTTAACGATCGTCATCTGCCGGATAAAGCCATTGATGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGA‑GTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGGGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTG‑GAAATGCTGGTCTTCGGTCAGGATAAAG‑CCAt                                                                                                                                            >  1:542418/1‑236 (MQ=255)
                                                                                       aTCGTCATCTGCCGGATAAAGCCATTGATGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGA‑GTCCGTGGTGGCCCGTATTGCACGCATGCCACAGAAGAGTGTTTTTCAGAGTGATCGTGATACCCTGTAAAACCCCGGCGATCGCTTG‑TAAATGCTGGGCTTCGGTCAGGGTACAG‑CCACTgt                                                                                                                                         >  2:77956/1‑231 (MQ=255)
                                                                                         cGTCATCTGCCGGATAAAGCCATTGATGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGA‑GTCCGTGGTGGCCCGTATTTCACGCATGCCAGAGAAGAGTGGTTCTCAAAGTGATCGTGATACCCTGAAAAACCTCGGCGACCGCTTT‑ACAATGCTGGTCTTCGGGCAGGATACAG‑CTATTGAgggg                                                                                                                                     >  2:544844/1‑232 (MQ=255)
                                                                                              tCTGCCGGATAAAGCCATTGATGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGA‑GTCCGTGGTGGCCCGGATTGCACGCATGGCCGAGAAAAGTGTTTTTCAGAGTGATCGTGATACCCCGAAAAAACACGCCGATCGCTTG‑TAAATGCCTGTCTTTGGGCAGGCTAAAGCCCATTAAGgcgcgacc                                                                                                                                >  2:284061/1‑231 (MQ=255)
                                                                                               cTGCCGGATAAAGCCATTGATGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGA‑GTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTG‑AAAATGCTGGTCTTCGGTCAGGATAAAG‑CCATTGAGGCGctgactga                                                                                                                             <  1:395790/236‑1 (MQ=255)
                                                                                                               ttGATGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGA‑GTCCGTGGTGGCCCCTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAAACTCGGCGATAGCTTG‑TAAATGCTGGGTTTCGGTCAGGATAAAG‑CCATTTAGGCGCTGCCTGAAGCCATTTAGATgtgg                                                                                                             >  2:32223/1‑233 (MQ=255)
                                                                                                                   tGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGA‑GTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTG‑AAAATGCTGGTCTTCGGTCAGGATAAAG‑CCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTg                                                                                                         <  1:83367/236‑1 (MQ=255)
                                                                                                                                       cTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGA‑GTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTG‑AAAATGCTGGTCTTCGGTCAGGATAAAG‑CCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACa                                                                                      <  1:284061/235‑1 (MQ=255)
                                                                                                                                         cgcgCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGA‑GTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTG‑AAAATGCTGGTCTTCGGTCAGGATAAAG‑CCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATa                                                                                    <  1:32223/235‑1 (MQ=255)
                                                                                                                                                            tAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGA‑GTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTG‑AAAATGCTGGTCTTCGGTCAGGATAAAG‑CCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGgttc                                                                       >  1:653925/1‑229 (MQ=255)
                                                                                                                                                                    cGCAAGAAAACCGTTAATGTGGCGGATATTGA‑GTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTG‑AAAATGCTGGTCTTCGGTCAGGATAAAG‑CCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGGTTCGTTCCTGTTTGCCgg                                                        <  2:653925/236‑1 (MQ=255)
                                                                                                                                                                               cGTTAATGTGGCGGATATTGA‑GTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTG‑AAAATGCTGGTCTTCGGTCAGGATAAAG‑CCATTGAGgcg                                                                                                                                     >  1:409597/1‑148 (MQ=255)
                                                                                                                                                                               cGTTAATGTGGCGGATATTGA‑GTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTG‑AAAATGCTGGTCTTCGGTCAGGATAAAG‑CCATTGAGgcg                                                                                                                                     <  2:409597/148‑1 (MQ=255)
                                                                                                                                                                                         gCGGATATTGA‑GTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTG‑AAAATGCTGGTCTTCGGt                                                                                                                                                           >  1:537573/1‑117 (MQ=255)
                                                                                                                                                                                         gCGGATATTGA‑GTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTG‑AAAATGCTGGTCTTCGGt                                                                                                                                                           <  2:537573/117‑1 (MQ=255)
                                                                                                                                                                                             ataattggctCCGTTGTCGCACGTATTGACCGCATGCCAGAGAGGAGGTTTTCTCAGAATGATCGTGATGCCCTGAAAAACCTCGGCGATCGCTTG‑AAAAGGCTGGTCTGCGGTCAGGATAAAG‑CCATTGAGGCGGTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGGTGGTTCGTTCCTGTTTGCCGGCCCTACCGGGGTCGGGAAAACaga                                <  2:531244/227‑1 (MQ=255)
                                                                                                                                                                                                ttGA‑GTCCGTGGTGGCCCGTATTGCACGCATGCCAGATAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTG‑AAAATGCTGGTCTTCGGTCAGGATAAAG‑CCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGGTTCGTTCCTGTTTGCCGGCCCTACCGGGGTCGGGAAAACAGAGGt                             <  1:192548/235‑1 (MQ=255)
                                                                                                                                                                                                 tGA‑GTCAGTGTGGGCCCGTATTGCCCGCATGCAAGAGAGGATTGTTTCTCAGAGTGATCGTGATACCCTGAAAACCCTCGGCGATCGCTTG‑AAAATGCTGGTCTTCGGTCAGGATAAAG‑CCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGGTTCGTTCCTGTTTGCCGGCCCTACCGGGGTCGGGAAAACAGAGGTGa                           <  2:425777/236‑1 (MQ=255)
                                                                                                                                                                                                   a‑GTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTG‑AAAATGCTGGTCTTCGGTCAGGATAAAG‑CCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGGTTCGTTCCTGCTTGCCGGCCCTACCGGGGTCGGGAAAACAGAGGTGACg                         >  2:187466/1‑236 (MQ=255)
                                                                                                                                                                                                     gTCCGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTG‑AAAATGCTGGTCTTCGGTCAGGATAAAG‑CCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGGGTCGTTCCTGTTTGCCGGCCCTACCGGGGTCGGGAAAAAAAGGGTGACgg                        >  2:410116/1‑236 (MQ=255)
                                                                                                                                                                                                       ccGTGGTGGCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTG‑AAAATGCTGGTCTTCGGTCAGGATAAAG‑CCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGGTTCGTTCCTGTTTGCCGGCCCTACCGGGGTCGGGAAAACAGAGGTGACGGTa                      <  1:77956/236‑1 (MQ=255)
                                                                                                                                                                                                           ggtggCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTG‑AAAATGCTGGTCTTCGGTCAGGATAAAG‑CCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGATTCGTTCCTGTTTGCCGGCCCTACCGGGGTCGGGAAAACAGAGGTGACGGTACAg                   <  2:696509/235‑1 (MQ=255)
                                                                                                                                                                                                              ggCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAGAACCTCGGCGATCGCTTG‑AAAATGCTGGTCTTCGGTCAGGATAAAG‑CCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGGTTCGTTCCTGTTTGCCGGCCCTACCGGGGTCGGGAAAACAGAGGTGACGGTACAGCttt               >  1:388240/1‑236 (MQ=255)
                                                                                                                                                                                                              ggCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTG‑AAAATGCTGGTCTTCGGTCAGGATAAAG‑CCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGGTTCGTTCCTGTTTGCCGGCCCTACCGGGGTCGGGGAACCCGAGGGGACGGTACAGCttt               >  2:630536/1‑236 (MQ=255)
                                                                                                                                                                                                              ggCCCGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTG‑AAAATGCTGGTCTTCGGTCAGGATAAAG‑CCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGGTTCGTTCCTGTTTGCCGGCCCTACCGGGGTCGGGAAAACATAGGGTAAGGTAACGCttt               >  2:143547/1‑236 (MQ=255)
                                                                                                                                                                                                                cccGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTG‑AAAATGCTGGTCTTCGGTCAGGATAAAG‑CCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGGTTCGTTCCTGTTTGCCGGCCCTACCGGGGCTGGGAAACCAGAGGTGACGGTACacacttcg             >  2:746190/1‑229 (MQ=255)
                                                                                                                                                                                                                 ccGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAGCCTCGGCGATCGCTTG‑AAAATGCTGGTCTTCGGTCAGGATAAAG‑CCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGGTTCGTTCCTGTTTGCCGGCCCTACCGGGGTCGGGAAAACAGAGGTGACGGTACAGCTTTCGa            <  1:143547/236‑1 (MQ=255)
                                                                                                                                                                                                                 ccGTATTGCACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTG‑AAAATGCTGGTCTTCGGTCAGGATAAAG‑CCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGGTTCGTTCCTGTTTGCCGGCCCTACCGGGGTCGGGAAAACAGAGGTGACGGTACAGCTTTCGa            <  1:630536/236‑1 (MQ=255)
                                                                                                                                                                                                                         cACGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTG‑AAAATGCTGGTCTTCGGTCAGGATAAAG‑CCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGGTTCGTTCCTGTTTGCCGGCCCTACCGGGGTCGGGAAAACAGAGGTGACGGTACAGCTTTCGAAAGCTTTg    >  1:343357/1‑236 (MQ=255)
                                                                                                                                                                                                                           cGCATGCCAGAGAAGAGTGTTTCTCAGCGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTG‑AAAATGCTGGTCTTCGGTCAGGATAAAG‑CCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGGTTCGTTCCTGTTTGCCGGCCCTACCGGGTCCGGGAAAACAGAGTGTCCGGTACAGCTTTCTACAGCTTTggg  >  1:376546/1‑236 (MQ=255)
                                                                                                                                                                                                                           cGCATGCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTG‑AAAATGCTGGTCTTCGGTCAGGATAAAG‑CCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGGTTCGTTCCTGTTTGCCGGCCCTACCGGGGTCGGGAAAACAGAGGTGACGGTACAGCTTTCGAAAGCTTTggg  >  1:750114/1‑236 (MQ=255)
                                                                                                                                                                                                                                |                                                                                                                                                                                                                                        
AACCGAAGTATGAAGCGCACCACGACGTGCGTTATACCGCAAAAGCGGTGCGTGCGGCGGTAGAGCTGGCGGTGAAATACATTAACGATCGTCATCTGCCGGATAAAGCCATTGATGTTATCGACGAAGCGGGCGCTCGCGCACGCCTGATGCCGGTAAGCAAACGCAAGAAAACCGTTAATGTGGCGGATATTGA‑GTCCGTGGTGGCCCGTATTGCACGCATTCCAGAGAAGAGTGTTTCTCAGAGTGATCGTGATACCCTGAAAAACCTCGGCGATCGCTTG‑AAAATGCTGGTCTTCGGTCAGGATAAAG‑CCATTGAGGCGCTGACTGAAGCCATTAAGATGGCGCGTGCAGGTTTAGGTCACGAACATAAACCGGTTGGTTCGTTCCTGTTTGCCGGCCCTACCGGGGTCGGGAAAACAGAGGTGACGGTACAGCTTTCGAAAGCTTTGGG  >  NC_000913/924348‑924801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A